| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,474,821 | T→G | 17.2% | intergenic (+119/‑115) | nrdA → / → nrdB | ribonucleoside diphosphate reductase 1, alpha subunit/ribonucleoside diphosphate reductase 1, beta subunit, ferritin‑like |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,474,821 | 0 | T | G | 17.2% | 73.7 / 13.1 | 35 | intergenic (+119/‑115) | nrdA/nrdB | ribonucleoside diphosphate reductase 1, alpha subunit/ribonucleoside diphosphate reductase 1, beta subunit, ferritin‑like |
| Reads supporting (aligned to +/- strand): ref base T (13/16); new base G (3/3); total (16/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.84e-01 | |||||||||||
TTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCTCCGGGTGCCGGATGCAGCGTGAACGCCTTATCCGGCCTAC‑‑‑‑GGCTCG‑G‑‑A‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATGCGGC > minE/1474754‑1474891 | ttGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCTCCGGGTGCCGGATGCAGCGTGAACGCCTTATc > 1:2745955/1‑71 (MQ=255) ggCCTGATAAGACGCGCCAGCGTCGCATCAGGCTCCGGGTGCCGGATGCAGCGTGAACGCCTTATCCGGc < 1:879386/70‑1 (MQ=255) gCCTGATAAGACGCGCCAGCGTCGCATCAGGCTCCGGGTGCCGGATGCAGCGTGAACGCCTTATCCGGcc > 1:3216386/1‑70 (MQ=255) ccTGATAAGACGCGCCAGCGTCGCATCAGGCTCCGGGTGCCGGATGCAGCGTGAACGCCTTATCCGGCCTa < 1:1978563/71‑1 (MQ=255) gATAAGACGCGCCAGCGTCGCATCAGGCTCCGGGTGCCGGATGCAGCGTGAACGCCTTATCCGGCCTAC‑‑‑‑g > 1:1061637/1‑70 (MQ=255) tAAGACGCGCCAGCGTCGCATCAGGCTCCGGGTGCCGGATGCAGCGTGAACGCCTTATCCGGCCTAC‑‑‑‑GGCt > 1:1044833/1‑71 (MQ=255) tAAGACGCGCCAGCGTCGCATCAGGCTCCGGGTGCCGGATGCAGCGTGAACGCCTTATCCGGCCTAC‑‑‑‑GGCt > 1:619846/1‑71 (MQ=255) aGACGCGCCAGCGTCGCATCAGGCTCCGGGTGCCGGATGCAGCGTGAACGCCTTATCCGGCCTAC‑‑‑‑GGCTCg < 1:3726169/71‑1 (MQ=255) gTCGCATCAGGCTCCGGGTGCCGGATGCAGCGTGAACGCCTTATCCGGCCTAC‑‑‑‑GGCTCG‑G‑‑A‑TTTGTAGGcc > 1:3649567/1‑71 (MQ=255) cATCAGGCTCCGGGTGCCGGATGCAGCGTGAACGCCTTATCCGGCCTAC‑‑‑‑GGCTCG‑G‑‑A‑TTTGTAGGCCTGAt < 1:563601/71‑1 (MQ=255) cATCAGGCTCCGGGTGCCGGATGCAGCGTGAACGCCTTATCCGGCCTAC‑‑‑‑GGCTCG‑G‑‑A‑TTTGTAGGCCTGAt < 1:1550958/71‑1 (MQ=255) tCAGGCTCCGGGTGCCGGATGCAGCGTGAACGCCTTATCCGGCCTAC‑‑‑‑GGCTCG‑G‑‑A‑TTTGTAGGCCTGAt > 1:1891044/1‑69 (MQ=255) aGGCTCCGGGTGCCGGATGCAGCGTGAACGCCTTATCCGGCCTAC‑‑‑‑GGCTCG‑G‑‑A‑TTTGTAGGCCTGATAAg < 1:278203/70‑1 (MQ=255) ggCTCCGGGTGCCGGATGCAGCGTGAACGCCTTATCCGGCCTAC‑‑‑‑GGCTCG‑G‑‑A‑TTTGTAGGCCTGATAAGAc > 1:549119/1‑71 (MQ=255) cTCCGGGTGCCGGATGCAGCGTGAACGCCTTATCCGGCCTAC‑‑‑‑GGCTCG‑G‑‑A‑TTTGTAGGCCTGATAAGAcgc < 1:1673135/71‑1 (MQ=255) gTGCCGGATGCAGCGTGAACGCCTTATCCGGCCTAC‑‑‑‑GGCTCG‑G‑‑A‑TTTGTAGGCCTGATa < 1:844341/59‑1 (MQ=255) gTGCCGGATGCAGCGTGAACGCCTTATCCGGCCTAC‑‑‑‑GGCTCG‑G‑‑A‑TTTGTAGGCCTGATAAGACGCGCCAGc > 1:139728/1‑71 (MQ=255) gTGCCGGATGCAGCGTGAACGCATTATCCGGCCTAC‑‑‑‑GGCTCG‑G‑‑A‑TTTGTAGGCCTGATAAGACGCGCCAGc > 1:1755111/1‑71 (MQ=255) ggATGCAGCGTGAACGCCTTATCCGGCCTAC‑‑‑‑GGCTCG‑G‑‑A‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCg > 1:2861943/1‑70 (MQ=255) gATGCAGCGTGAACGCCTTATCCGGCCTAC‑‑‑‑GGCTCG‑G‑‑A‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCa < 1:2643893/71‑1 (MQ=255) gATGCAGCGTGAACGCCTTATCCGGCCTAC‑‑‑‑GGCTCG‑G‑‑A‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCa > 1:3642708/1‑71 (MQ=255) aTGCAGCGTGAACGCCTTATCCGGCCTAC‑‑‑‑GGCTCG‑G‑‑A‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCAt < 1:1398706/71‑1 (MQ=255) aGCGTAAACGCCTGATCCGTCCAAC‑‑‑‑GGTTCG‑G‑‑ATTTTGTAGGCCTGATAAGAcgcg > 1:3524888/1‑56 (MQ=2) aGCGTAAACGCCTGATCCGTCCAAC‑‑‑‑GGTTCG‑G‑‑ATTTTGTAGGCCTGATAAGACGCGGTAGCGTCGCATCAg > 1:3457726/1‑71 (MQ=2) gCGTGAACGCCTTATCCGGCCTACGGATGGCGCG‑GGAA‑TTTGTAGGCCTGATAAGACGCGTTAGCGTCGCa < 1:2546502/71‑1 (MQ=0) cGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGA‑A‑TTTGTAGGCCTGATAAGACGCGTTAGCGTCGCAt > 1:3206446/1‑71 (MQ=0) taaaCGCCTGATCCGTCCAAC‑‑‑‑GGTTCG‑G‑‑ATTTTGTAGGCCTGATAAGACGCGGTAGCGTCGCATCAGGcat < 1:3519389/69‑2 (MQ=2) aaCGCCTTATCCGGCCTAC‑‑‑‑GGCTCG‑G‑‑A‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAgg < 1:1702689/71‑1 (MQ=255) aaCGCCTTATCCGGCCTAC‑‑‑‑GGCTCG‑G‑‑A‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAg < 1:2833867/70‑1 (MQ=255) aaCGCCTTATCCGGCCTAC‑‑‑‑GGCTCG‑G‑‑A‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAg < 1:3225702/70‑1 (MQ=255) aaCGCCTGATCCGTCCAAC‑‑‑‑GGTTCG‑G‑‑ATTTTGTAGGCCTGATAAGACGCGGTAGCGTCGCATCAGGcatct < 1:1790819/71‑4 (MQ=2) aaCGCCTGATCCGTCCAAC‑‑‑‑GGTTCG‑G‑‑ATTTTGTAGGCCTGATAAGACGCGGTAGCGTCGCATCAGGcatc < 1:3415668/70‑3 (MQ=2) aCGCCTGATCCGTCCAAC‑‑‑‑GGTTCG‑G‑‑ATTTTGTAGGCCTGATAAGACGCGGTAGCGTCGCAt > 1:2284845/1‑61 (MQ=2) gCCTTATCCGGCCTAC‑‑‑‑GGCTCG‑G‑‑A‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATg < 1:3217889/71‑1 (MQ=255) gCCTTATCCGGCCTAC‑‑‑‑GGCTCG‑G‑‑A‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATg < 1:2401244/71‑1 (MQ=255) tATCCGGCCTAC‑‑‑‑GGCTCG‑G‑‑A‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATgcggc > 1:3370307/1‑71 (MQ=255) tATCCGGCCTAC‑‑‑‑GGCTCG‑G‑‑A‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATgcggc > 1:1032332/1‑71 (MQ=255) | TTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCTCCGGGTGCCGGATGCAGCGTGAACGCCTTATCCGGCCTAC‑‑‑‑GGCTCG‑G‑‑A‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATGCGGC > minE/1474754‑1474891 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |