| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,474,835 | C→T | 16.2% | intergenic (+133/‑101) | nrdA → / → nrdB | ribonucleoside diphosphate reductase 1, alpha subunit/ribonucleoside diphosphate reductase 1, beta subunit, ferritin‑like |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,474,835 | 0 | C | T | 16.2% | 96.1 / 8.4 | 37 | intergenic (+133/‑101) | nrdA/nrdB | ribonucleoside diphosphate reductase 1, alpha subunit/ribonucleoside diphosphate reductase 1, beta subunit, ferritin‑like |
| Reads supporting (aligned to +/- strand): ref base C (13/18); new base T (3/3); total (16/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TAAGACGCGCCAGCGTCGCATCAGGCTCCGGGTGCCGGATGCAGCGTGAACGCCTTATCCGGCCTAC‑‑‑‑GGCTCG‑G‑‑A‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATGCGGCGTAAAATGCCTTAT > minE/1474766‑1474905 | tAAGACGCGCCAGCGTCGCATCAGGCTCCGGGTGCCGGATGCAGCGTGAACGCCTTATCCGGCCTAC‑‑‑‑GGCt > 1:1044833/1‑71 (MQ=255)tAAGACGCGCCAGCGTCGCATCAGGCTCCGGGTGCCGGATGCAGCGTGAACGCCTTATCCGGCCTAC‑‑‑‑GGCt > 1:619846/1‑71 (MQ=255) aGACGCGCCAGCGTCGCATCAGGCTCCGGGTGCCGGATGCAGCGTGAACGCCTTATCCGGCCTAC‑‑‑‑GGCTCg < 1:3726169/71‑1 (MQ=255) gTCGCATCAGGCTCCGGGTGCCGGATGCAGCGTGAACGCCTTATCCGGCCTAC‑‑‑‑GGCTCG‑G‑‑A‑TTTGTAGGcc > 1:3649567/1‑71 (MQ=255) cATCAGGCTCCGGGTGCCGGATGCAGCGTGAACGCCTTATCCGGCCTAC‑‑‑‑GGCTCG‑G‑‑A‑TTTGTAGGCCTGAt < 1:563601/71‑1 (MQ=255) cATCAGGCTCCGGGTGCCGGATGCAGCGTGAACGCCTTATCCGGCCTAC‑‑‑‑GGCTCG‑G‑‑A‑TTTGTAGGCCTGAt < 1:1550958/71‑1 (MQ=255) tCAGGCTCCGGGTGCCGGATGCAGCGTGAACGCCTTATCCGGCCTAC‑‑‑‑GGCTCG‑G‑‑A‑TTTGTAGGCCTGAt > 1:1891044/1‑69 (MQ=255) aGGCTCCGGGTGCCGGATGCAGCGTGAACGCCTTATCCGGCCTAC‑‑‑‑GGCTCG‑G‑‑A‑TTTGTAGGCCTGATAAg < 1:278203/70‑1 (MQ=255) ggCTCCGGGTGCCGGATGCAGCGTGAACGCCTTATCCGGCCTAC‑‑‑‑GGCTCG‑G‑‑A‑TTTGTAGGCCTGATAAGAc > 1:549119/1‑71 (MQ=255) cTCCGGGTGCCGGATGCAGCGTGAACGCCTTATCCGGCCTAC‑‑‑‑GGCTCG‑G‑‑A‑TTTGTAGGCCTGATAAGAcgc < 1:1673135/71‑1 (MQ=255) gTGCCGGATGCAGCGTGAACGCCTTATCCGGCCTAC‑‑‑‑GGCTCG‑G‑‑A‑TTTGTAGGCCTGATa < 1:844341/59‑1 (MQ=255) gTGCCGGATGCAGCGTGAACGCCTTATCCGGCCTAC‑‑‑‑GGCTCG‑G‑‑A‑TTTGTAGGCCTGATAAGACGCGCCAGc > 1:139728/1‑71 (MQ=255) gTGCCGGATGCAGCGTGAACGCATTATCCGGCCTAC‑‑‑‑GGCTCG‑G‑‑A‑TTTGTAGGCCTGATAAGACGCGCCAGc > 1:1755111/1‑71 (MQ=255) ggATGCAGCGTGAACGCCTTATCCGGCCTAC‑‑‑‑GGCTCG‑G‑‑A‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCg > 1:2861943/1‑70 (MQ=255) gATGCAGCGTGAACGCCTTATCCGGCCTAC‑‑‑‑GGCTCG‑G‑‑A‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCa < 1:2643893/71‑1 (MQ=255) gATGCAGCGTGAACGCCTTATCCGGCCTAC‑‑‑‑GGCTCG‑G‑‑A‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCa > 1:3642708/1‑71 (MQ=255) aTGCAGCGTGAACGCCTTATCCGGCCTAC‑‑‑‑GGCTCG‑G‑‑A‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCAt < 1:1398706/71‑1 (MQ=255) aGCGTAAACGCCTGATCCGTCCAAC‑‑‑‑GGTTCG‑G‑‑ATTTTGTAGGCCTGATAAGAcgcg > 1:3524888/1‑56 (MQ=2) aGCGTAAACGCCTGATCCGTCCAAC‑‑‑‑GGTTCG‑G‑‑ATTTTGTAGGCCTGATAAGACGCGGTAGCGTCGCATCAg > 1:3457726/1‑71 (MQ=2) gCGTGAACGCCTTATCCGGCCTACGGATGGCGCG‑GGAA‑TTTGTAGGCCTGATAAGACGCGTTAGCGTCGCa < 1:2546502/71‑1 (MQ=0) cGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGA‑A‑TTTGTAGGCCTGATAAGACGCGTTAGCGTCGCAt > 1:3206446/1‑71 (MQ=0) taaaCGCCTGATCCGTCCAAC‑‑‑‑GGTTCG‑G‑‑ATTTTGTAGGCCTGATAAGACGCGGTAGCGTCGCATCAGGcat < 1:3519389/69‑2 (MQ=2) aaCGCCTTATCCGGCCTAC‑‑‑‑GGCTCG‑G‑‑A‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAgg < 1:1702689/71‑1 (MQ=255) aaCGCCTTATCCGGCCTAC‑‑‑‑GGCTCG‑G‑‑A‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAg < 1:2833867/70‑1 (MQ=255) aaCGCCTTATCCGGCCTAC‑‑‑‑GGCTCG‑G‑‑A‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAg < 1:3225702/70‑1 (MQ=255) aaCGCCTGATCCGTCCAAC‑‑‑‑GGTTCG‑G‑‑ATTTTGTAGGCCTGATAAGACGCGGTAGCGTCGCATCAGGcatct < 1:1790819/71‑4 (MQ=2) aaCGCCTGATCCGTCCAAC‑‑‑‑GGTTCG‑G‑‑ATTTTGTAGGCCTGATAAGACGCGGTAGCGTCGCATCAGGcatc < 1:3415668/70‑3 (MQ=2) aCGCCTGATCCGTCCAAC‑‑‑‑GGTTCG‑G‑‑ATTTTGTAGGCCTGATAAGACGCGGTAGCGTCGCAt > 1:2284845/1‑61 (MQ=2) gCCTTATCCGGCCTAC‑‑‑‑GGCTCG‑G‑‑A‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATg < 1:3217889/71‑1 (MQ=255) gCCTTATCCGGCCTAC‑‑‑‑GGCTCG‑G‑‑A‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATg < 1:2401244/71‑1 (MQ=255) tATCCGGCCTAC‑‑‑‑GGCTCG‑G‑‑A‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATgcggc > 1:3370307/1‑71 (MQ=255) tATCCGGCCTAC‑‑‑‑GGCTCG‑G‑‑A‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATgcggc > 1:1032332/1‑71 (MQ=255) gCCTAC‑‑‑‑GGCTCG‑G‑‑A‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATg < 1:2240725/61‑1 (MQ=255) ccTAC‑‑‑‑GGCTCG‑G‑‑A‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATGCGGCGTAAAAt > 1:237384/1‑71 (MQ=255) cTAC‑‑‑‑GGCTCG‑G‑‑A‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATGCGGCGTAAAATg < 1:2909558/71‑1 (MQ=255) tAC‑‑‑‑GGCTCG‑G‑‑A‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATGCGGCGTaa < 1:1571474/66‑1 (MQ=255) aC‑‑‑‑GGCTCG‑G‑‑A‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAg < 1:3692041/53‑1 (MQ=255) cTCG‑G‑‑A‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATGCGGCGTAAAATGCCTTAt < 1:2901458/71‑1 (MQ=255) | TAAGACGCGCCAGCGTCGCATCAGGCTCCGGGTGCCGGATGCAGCGTGAACGCCTTATCCGGCCTAC‑‑‑‑GGCTCG‑G‑‑A‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATGCGGCGTAAAATGCCTTAT > minE/1474766‑1474905 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |