| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,941,704 | C→A | 25.9% | intergenic (+143/+53) | ygfZ → / ← yqfA | predicted folate‑dependent regulatory protein/predicted oxidoreductase, inner membrane subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,941,704 | 0 | C | A | 25.9% | 163.3 / 38.9 | 77 | intergenic (+143/+53) | ygfZ/yqfA | predicted folate‑dependent regulatory protein/predicted oxidoreductase, inner membrane subunit |
| Reads supporting (aligned to +/- strand): ref base C (24/33); new base A (10/10); total (34/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.06e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.29e-01 | |||||||||||
ATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGC‑TGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATA > minE/1941639‑1941772 | aTCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATg < 1:346469/70‑1 (MQ=255)aTCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGc > 1:2374481/1‑71 (MQ=255) tCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCg < 1:2804215/66‑1 (MQ=255) acaacaGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTg < 1:3901166/71‑1 (MQ=255) caacaGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTg < 1:2732458/70‑1 (MQ=255) acaGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATg > 1:3508359/1‑71 (MQ=255) caGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGc < 1:3592745/71‑1 (MQ=255) gggACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGa < 1:3009107/71‑1 (MQ=255) ggACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGa < 1:1252176/65‑1 (MQ=255) ggACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGa < 1:3084413/65‑1 (MQ=255) ggatcggtaggccggataaggcgtttatgccgcATCCGGCAGTTATGCGCAGATGCCTGATGCGACGC‑Tgg > 1:3895929‑M2/34‑71 (MQ=255) ggCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGC‑TGgc < 1:214341/71‑1 (MQ=255) gatcggtaggccggataaggcgtttatgccgcATCCGGCAGTTATGCGCAGATGCCTGATGCGACGC‑TGgc > 1:1389578‑M2/33‑71 (MQ=255) gatcggtaggccggataaggcgtttatgccgcATCCGGCAGTTATGCGCAGATGCCTGATGCGACGC‑TGgc < 1:1573900‑M2/39‑1 (MQ=255) gatcggtaggccggataaggcgtttatgccgcATCCGGCAGTTATGCGCAGATGCCTGATGCGACGC‑TGgc < 1:3416521‑M2/39‑1 (MQ=255) cccGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGC‑TGgcgc < 1:1035159/71‑1 (MQ=255) cGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGC‑TGgc > 1:721399/1‑67 (MQ=255) gtaggccggataaggcgtttatgccgcATCCGGCAGTTATGCGCAGATGCCTGATGCGACGC‑TGGCGCGTc < 1:2360204‑M2/44‑1 (MQ=255) gTAGATCGGATAAGGCTTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGC‑TGGCGCGTc < 1:680415/71‑1 (MQ=255) gTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGC‑Tg > 1:1105797/1‑64 (MQ=255) gTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGC‑TGgcgc > 1:1006972/1‑68 (MQ=255) gTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGC‑TGGCGCGTc > 1:3656583/1‑71 (MQ=255) tAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGC‑TGGCGCGTCt > 1:1806659/1‑71 (MQ=255) tAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGC‑TGGCGCGTCt > 1:3580549/1‑71 (MQ=255) aGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGC‑TGGCGCGTCt < 1:1056558/70‑1 (MQ=255) aGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGC‑TGGCGCGTCt > 1:343487/1‑70 (MQ=255) aGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGC‑TGGCGCGTCt > 1:870230/1‑70 (MQ=255) ggccggataaggcgttcacgccgcATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGC‑TAACGCGTCtt < 1:133151‑M2/46‑1 (MQ=255) gATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGC‑TGGCGCGTCtt < 1:3064611/70‑1 (MQ=255) gacggataaggcgtttacgccgcATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGC‑TGGCGCGTCTTAt < 1:1662265‑M2/48‑1 (MQ=255) gacggataaggcgtttacgccgcATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGC‑TGGCGCGTCTTAt < 1:2368052‑M2/48‑1 (MQ=255) ccggaaaaggcgttcacgccgcATCCGGCAGTCGTGCGCAGATGcc > 1:648346‑M2/23‑46 (MQ=255) cggataaggcgtttatgccgcATCCGGCAGTTATGCGCAGATGCCTGATGCGACGC‑TGGCGCGTCTTATCa > 1:987682‑M2/22‑71 (MQ=255) cggataaggcgttcacgccgcATCCGGCAGTCGTGCGCC‑ATGCCTGATGCGACGCTTTGCGCGTCTTATc < 1:2904295‑M2/49‑1 (MQ=255) cGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGC‑TGgc < 1:2766836/60‑1 (MQ=255) ggataaggcgttcacgccgcATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGC‑TAACGCGTCTTATCAg < 1:1554587‑M2/51‑1 (MQ=255) ggATAAGGTGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGC‑TGGCGCGTCTTATCAg > 1:301015/1‑71 (MQ=255) ggATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGC‑TGGCGCGTCTTATCAg > 1:3441355/1‑71 (MQ=255) ggATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGC‑TGGCGCGTCTTATCAg < 1:101681/71‑1 (MQ=255) gataaggcgtttatgccgcATCCGGCAGTTATGCGCAGATGCCTGATGCGACGC‑TGGCGCGTCTTAtcatg < 1:1401336‑M2/52‑3 (MQ=255) gataaggcgtttatgccgcATCCGGCAGTTATGCGCAGATGCCTGATGCGACGC‑TGGCGCGTCTTAtcatg > 1:3861018‑M2/20‑69 (MQ=255) gATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGC‑TTGCGCGTCTTATCAgg > 1:3149341/1‑71 (MQ=255) ataaggcgttcacgccgcATCCGGCAGTTGTGCACCAATgct < 1:3276618‑M2/24‑2 (MQ=255) aTAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGC‑TGGCGCGTCTTATCAGGc > 1:2086588/1‑71 (MQ=255) taaggcgttcacgccgcATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGC‑t > 1:283239‑M2/18‑53 (MQ=255) taaggcgttcacgccgcATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGC‑TAACGCGTCTTATCAGAcc < 1:3457494‑M2/54‑1 (MQ=255) aaggcgttcacgccgcATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGC‑TAACGCGTCTTATCAGAcc > 1:230984‑M2/17‑70 (MQ=255) ggCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGC‑TGGCGCGTCTTATCAGGCCTa > 1:3348871/1‑70 (MQ=255) gcgttcacgccgcATCCGGCAGTCGTGCGCC‑ATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTAc < 1:877651‑M2/57‑1 (MQ=255) cgttcacgccgcATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGC‑TAACGCGTCTTATCAGGCCTACaa < 1:1261151‑M2/59‑1 (MQ=255) cGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGC‑TGGCGCGTCTTATCAGGCCTACaa < 1:803310/71‑1 (MQ=255) cGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGC‑TGGCGCGTCTTATCAGGCCTACaa < 1:2028191/71‑1 (MQ=255) gTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGC‑TGGCGCGTCTTATCAGGCCTACaaa < 1:225786/71‑1 (MQ=255) gTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGC‑TGGCGCGTCTTATCAGGCCTACa > 1:1005245/1‑69 (MQ=255) ttCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGC‑TGGCGCGTCTTATCAGGCCTACAAAg < 1:1800337/71‑1 (MQ=255) ttCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGC‑TGGCGCGTCTTATCAGGCCTACAAAg > 1:3858277/1‑71 (MQ=255) tcacgccgcATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGC‑TAACGCGTCTTATCAGACCTacaaatt > 1:3835603‑M2/10‑69 (MQ=255) tCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGAt < 1:1322856/37‑1 (MQ=255) cACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGC‑TGGCGCGTCTTATCAGGCCTACAAAgg > 1:335590/1‑70 (MQ=255) cACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGC‑TGGCGCGTCTTATCAGGCCTACAAAgg > 1:370796/1‑70 (MQ=255) aCGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGC‑TGGCGCGTCTTATCAGGCCTACAAAGGCa > 1:2875286/1‑71 (MQ=255) ccgcATCCGGCAGTTATGCGCAGATGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTAc > 1:876036‑M2/5‑61 (MQ=255) ccGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGC‑TGGCGCGTCTTATCAGGCCTACAAAGGCATAc > 1:2790125/1‑71 (MQ=255) cgcATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTacg > 1:3914912‑M2/4‑60 (MQ=255) cGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGC‑TGGCGCGTCTTATCAGGCCTACAAAGGCATAcc > 1:3800541/1‑71 (MQ=255) aaTCCGGCAGTTGTGCTCCGATGCCTGATGCGACGC‑TGGCGCGTCTTATCAGGCCTACAAAGGCATACCCa > 1:2383334/1‑71 (MQ=255) tCCGGCAGTTGTGCTCCGATGCCTGATGCGACGC‑TGGCGCGTCTTATCAGGCCTACAAAGGCATACCCAtt < 1:1176943/71‑1 (MQ=255) cGGCAGTTGTGCTCCGATGCCTGATGTGACGC‑TGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTAc < 1:413252/71‑1 (MQ=255) cGGCAGTTGTGCTCCGATGCCTGATGCGACGC‑TGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTAc < 1:2323387/71‑1 (MQ=255) cGGCAGTTGTGCTCCGATGCCTGATGCGACGC‑TGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTAc < 1:3625777/71‑1 (MQ=255) cAGTTGTGCTCCGATGCCTGATGCGACGC‑TGGCGCGTCTTATCAGGCCTACAAAGGCCTACCCATTa > 1:584861/1‑67 (MQ=255) ttGTGCTCCGATGCCTGATGCGACGC‑TGGCGCGTCTTATCAGGCCTACaa < 1:1915082/50‑1 (MQ=255) gtgCTCCGATGCCTGATGCGACGC‑TGGCGCGTCTTATCAGGCCTACAAAGGCATAc < 1:1648832/56‑1 (MQ=255) gtgCTCCGATGCCTGATGCGACGC‑TGGCGCGTCTTATCAGGCCTACAAAGGCATAc < 1:61827/56‑1 (MQ=255) tgCTCCGATGCCTGATGCGACGC‑TGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCa < 1:579702/71‑1 (MQ=255) cTCCGATGCCTGATGCGACGC‑TGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCaa < 1:2030895/70‑1 (MQ=255) cTCCGATGCCTGATGCGACGC‑TGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCaa < 1:2755114/70‑1 (MQ=255) ccGATGCCTGATGCGACGC‑TGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAata > 1:1217115/1‑70 (MQ=255) cGATGCCTGATGCGACGC‑TGGCGCGTCTTATCAGGCCTACa > 1:2476518/1‑41 (MQ=32) | ATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGC‑TGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATA > minE/1941639‑1941772 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |