| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,045,818 | C→T | 10.4% | intergenic (+580/+215) | yqiK → / ← rfaE | conserved hypothetical protein/fused heptose 7‑phosphate kinase and heptose 1‑phosphate adenyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,045,818 | 0 | C | T | 10.4% | 143.2 / 5.7 | 48 | intergenic (+580/+215) | yqiK/rfaE | conserved hypothetical protein/fused heptose 7‑phosphate kinase and heptose 1‑phosphate adenyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (15/28); new base T (2/3); total (17/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.75e-01 | |||||||||||
AGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGT > minE/2045752‑2045887 | aGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTcc > 1:637868/1‑71 (MQ=255) cATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTccc < 1:2102473/70‑1 (MQ=255) aTTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCga < 1:3295006/71‑1 (MQ=255) cgGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGaagaag < 1:3649451/70‑1 (MQ=255) ggggTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAg‑gg < 1:3065948/71‑1 (MQ=255) gggTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAg‑gg < 1:904446/70‑1 (MQ=255) gccGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAG‑GGGCCt < 1:753594/70‑1 (MQ=255) cgcTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGtat < 1:2460908/71‑1 (MQ=255) cgcTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGta > 1:934496/1‑70 (MQ=255) gcTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGtata < 1:1240967/71‑1 (MQ=255) gcTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGtata < 1:893500/71‑1 (MQ=255) tcatcaACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGtat < 1:3810546/68‑1 (MQ=255) atcaACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGta > 1:299217/1‑65 (MQ=255) atcaACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAgg > 1:1848227/1‑70 (MQ=255) tcaACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAG‑GGGc < 1:3191778/58‑1 (MQ=255) caacaaTCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGa > 1:383422/1‑69 (MQ=255) gggggCAGCAAGGGGCGGAAACGGGAAAGCCCCTCCCGAAGAAG‑GGGCCtt < 1:2321566/51‑1 (MQ=255) ggggCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAg‑ggg < 1:10232/46‑1 (MQ=255) ggggCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGtat < 1:379648/54‑1 (MQ=255) ggggCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTatgat < 1:2822319/71‑1 (MQ=255) ggCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTAtgatga < 1:2250737/71‑1 (MQ=255) gcagcaAGGGGCTGAAACGGGAAAGCCCCTCCCgaaga < 1:424134/38‑1 (MQ=255) gcaAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCt > 1:3826505/1‑71 (MQ=255) caAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAg > 1:1959571/1‑68 (MQ=255) aaGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGaaa > 1:2838940/1‑53 (MQ=255) aaGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCg > 1:1376259/1‑71 (MQ=255) acgCTGAAACGGGAAAGCCTCTCCCGGAGAAGAGGGCTTTTAATAAGGAAAGGGTTATGATGAAGCACGt > 1:1708430/3‑70 (MQ=0) ggCTGAAACGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGtcat < 1:1936945/71‑1 (MQ=255) gCTGAAACGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTatgatg < 1:3100332/59‑1 (MQ=255) tGAAACGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCg < 1:748236/64‑1 (MQ=255) gAAACGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAgg > 1:3395419/1‑47 (MQ=255) aaaCGTGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTcatcat < 1:3550215/69‑1 (MQ=255) aaaCGGGAAAGCCTCTCCCGGAGAAGAGGGCTTTTAATAAGGAAAGGGTTAtgatga > 1:116466/1‑57 (MQ=0) aaaCGGGAAAGCCCCTCCCGAGGAAG‑GGGCCATAAATAAGGAAAGGGTCATGATGAAGCTACTcatcat < 1:3128594/69‑1 (MQ=11) aaaCGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGaaa < 1:2098056/44‑1 (MQ=255) aaaCGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTat < 1:3155389/51‑1 (MQ=255) aaaCGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTcatcat < 1:2923315/69‑1 (MQ=255) aaaCGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATAc > 1:673150/1‑71 (MQ=255) aaCGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATAc < 1:2633942/70‑1 (MQ=255) aCGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTTTATAAg > 1:3420149/1‑38 (MQ=255) aCGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTc > 1:3282549/1‑59 (MQ=255) aCGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTc > 1:2261239/1‑59 (MQ=255) gggAAAGCCTCTCCCGGAGAAGAGGGCTTTTAATAAGGAAAGGGTTATGATGAAGCACGTCATCATACTgg < 1:1049747/71‑1 (MQ=11) gggAAAGCCTCTCCCGGAGAAGAGGGCTTTTAATAAGGAAAGGGTTATGATGAAGCACGTCATCATACTgg < 1:3376571/71‑1 (MQ=11) ggAAAGCCTCTCCCGGAGAAGAGGGCTTTTAATAAGGAAAGGGTTa < 1:3541859/46‑1 (MQ=0) gAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgttg > 1:2125140/1‑71 (MQ=255) aaaGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgttgt < 1:484967/71‑1 (MQ=255) aGCCCCTCCCGAGGAAG‑GGGCCATAAATAAGGAAAGGGTCATGATGAAGCTACTcatcatc < 1:1334196/61‑2 (MQ=9) cccTCCCGAGGAAG‑GGGCCATAAATAAGGAAAGGGTCATGATGAAGCTACTcatcatcg < 1:994508/59‑3 (MQ=255) cccTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAg < 1:273649/46‑1 (MQ=255) cccTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgttgtgttgt < 1:3639705/71‑1 (MQ=255) | AGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGT > minE/2045752‑2045887 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |