Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,571,326 T→C 15.5% intergenic (+94/‑1685) yibH → / → ysaB hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,571,3260TC15.5% 173.5 / 22.2 71intergenic (+94/‑1685)yibH/ysaBhypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (21/39);  new base C (8/3);  total (29/42)
Fisher's exact test for biased strand distribution p-value = 4.13e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01

CTTTGCTCCTTGTAAGGACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGC  >  minE/2571256‑2571392
                                                                       |                                                                   
cTTTGCTCCTTGTAAGGACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATAtt                                                                     <  1:3917052/71‑1 (MQ=255)
   tGCTCCTTGTAAGGACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTc                                                                    <  1:3390868/69‑1 (MQ=255)
   tGCTCCTTGTAAGGACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCa                                                                   >  1:3033259/1‑70 (MQ=255)
   tGCTCCTTGTAAGGACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCAt                                                                  >  1:232100/1‑71 (MQ=255)
   tGCTCCTTGTAAGGACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCAt                                                                  >  1:1758303/1‑71 (MQ=255)
     cTCCTTGTAAGGACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTa                                                                <  1:737063/71‑1 (MQ=255)
        cTTGTAAGGACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCAtt                                                                 <  1:848762/67‑1 (MQ=255)
        cTTGTAAGGACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCAt                                                                  <  1:2139544/66‑1 (MQ=255)
           gTAAGGACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCa                                                                   <  1:1386381/62‑1 (MQ=255)
           gTAAGGACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTtaa                                                          >  1:450382/1‑71 (MQ=255)
            tAAGGACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTtaa                                                          <  1:921862/70‑1 (MQ=255)
             aaGGACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTtaat                                                         <  1:3318810/70‑1 (MQ=255)
              aGGACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTtaataa                                                       <  1:2593480/71‑1 (MQ=255)
               ggACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAg                                                      <  1:78859/71‑1 (MQ=255)
                gACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGt                                                     <  1:1057921/71‑1 (MQ=255)
                gACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGt                                                     <  1:707929/71‑1 (MQ=255)
                 aCTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGt                                                     <  1:707223/70‑1 (MQ=255)
                   tAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATAtt                                                                     <  1:2904910/52‑1 (MQ=255)
                   tAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTtaa                                                          >  1:3075837/1‑63 (MQ=255)
                     attattTTTGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTtaata                                                        >  1:106744/1‑63 (MQ=255)
                     attattTTTGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑Tg                                                <  1:2119564/70‑1 (MQ=255)
                      ttattTTTGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGt                                                             >  1:477513/1‑57 (MQ=255)
                      ttattTTTGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGa                                              <  1:1232140/71‑1 (MQ=255)
                      ttattTTTGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGa                                              <  1:2225942/71‑1 (MQ=255)
                       tattTTTGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGAt                                             >  1:85200/1‑71 (MQ=255)
                       tattTTTGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGAt                                             >  1:418124/1‑71 (MQ=255)
                       tattTTTGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGAt                                             >  1:2182597/1‑71 (MQ=255)
                        attTTTGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGAt                                             >  1:2254309/1‑70 (MQ=255)
                         tttttGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATg                                            <  1:3533469/70‑1 (MQ=255)
                           tttGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAg                                          <  1:2502571/70‑1 (MQ=255)
                             tGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAgtg                                        <  1:2389877/70‑1 (MQ=255)
                              gCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAgtgt                                       >  1:1360047/1‑70 (MQ=255)
                               cATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTc                                                                    <  1:20619/41‑1 (MQ=255)
                                atatTACTTCATTAGCTTCGATTATTTTT‑CTTTAATATTCATTATGTTAATAAGtt                                                    >  1:1502246/1‑56 (MQ=255)
                                atatTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTAct                                    <  1:2694549/71‑1 (MQ=255)
                                 tatTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCAt                                                                  <  1:1662302/41‑1 (MQ=255)
                                  atTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTtaata                                                        <  1:752619/50‑1 (MQ=255)
                                   ttACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGctct                                  <  1:175902/70‑1 (MQ=255)
                                     aCTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCa                                <  1:1985376/70‑1 (MQ=255)
                                     aCTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCa                                <  1:3027968/70‑1 (MQ=255)
                                      cTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTtaataa                                                       >  1:1194867/1‑47 (MQ=255)
                                      cTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCAtt                              <  1:2485622/71‑1 (MQ=255)
                                         cATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGa                                              >  1:1637292/1‑52 (MQ=255)
                                         cATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTg                             <  1:2992931/69‑1 (MQ=255)
                                         cATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGa                           >  1:2008979/1‑71 (MQ=255)
                                          aTTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGGGTGCTCTCATTGGAt                          <  1:2791507/71‑1 (MQ=255)
                                           ttAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAg                                                      <  1:2789876/43‑1 (MQ=255)
                                           ttAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑Tg                                                <  1:1884285/48‑1 (MQ=255)
                                           ttAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑Tg                                                <  1:909931/48‑1 (MQ=255)
                                             aGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGa                                              >  1:2105731/1‑48 (MQ=255)
                                               cTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGAtttttt                     <  1:1410050/71‑1 (MQ=255)
                                               cTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGAtttttt                     <  1:1809107/71‑1 (MQ=255)
                                               cTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGAtttttt                     <  1:51191/71‑1 (MQ=255)
                                                 tCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAgt                                         >  1:1241951/1‑49 (MQ=255)
                                                 tCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAgt                                         >  1:2625243/1‑49 (MQ=255)
                                                 gtgatgatTTTTCCTTTAATATCCAGCATGTTAAGAAGTTC‑t                                                 >  1:1733264/3‑42 (MQ=12)
                                                 gtgatgatTTTTCCTTTAATATCCAGCATGTTAAGAAGTTC‑Tg                                                >  1:2571275/3‑43 (MQ=16)
                                                  cGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATc                  >  1:466854/1‑71 (MQ=255)
                                                   gatgatTTTTCCTTTAATATCCAGCATGTAAAGAAGTTc                                                   >  1:2962167/1‑39 (MQ=9)
                                                    atgatTTTT‑CTTTAATATTCATTATGTTAATAAGtt                                                    <  1:1363014/36‑1 (MQ=255)
                                                    atgatTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCg                <  1:1035998/71‑1 (MQ=255)
                                                     tgatTTTTCCTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATcc                 >  1:602030/1‑71 (MQ=255)
                                                      gatTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTc              >  1:334079/1‑71 (MQ=255)
                                                       atTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTc              <  1:833991/70‑1 (MQ=255)
                                                            tccTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTc              >  1:1663272/3‑67 (MQ=255)
                                                            tccTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTg           <  1:1699436/68‑1 (MQ=255)
                                                             ccTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTga          <  1:2002593/69‑2 (MQ=255)
                                                              cTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTACCCGTCTTGATc        <  1:681628/71‑1 (MQ=255)
                                                              atttAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTGATc        >  1:1204721/2‑71 (MQ=255)
                                                                ttAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTGATCTg      >  1:1385251/1‑71 (MQ=255)
                                                                 tAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTGATCTgt     >  1:3859165/1‑70 (MQ=255)
                                                                    tatTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGAt                          <  1:2954658/46‑1 (MQ=255)
                                                                    tatTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTAACCGTCTTGTTCTGCCGc  <  1:1658843/70‑1 (MQ=255)
                                                                       |                                                                   
CTTTGCTCCTTGTAAGGACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGC  >  minE/2571256‑2571392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑