Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,571,330 T→C 17.2% intergenic (+98/‑1681) yibH → / → ysaB hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,571,3300TC17.2% 145.1 / 22.4 64intergenic (+98/‑1681)yibH/ysaBhypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (18/35);  new base C (8/3);  total (26/38)
Fisher's exact test for biased strand distribution p-value = 3.91e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.80e-01

CTCCTTGTAAGGACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGA  >  minE/2571261‑2571399
                                                                      |                                                                      
cTCCTTGTAAGGACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTa                                                                       <  1:737063/71‑1 (MQ=255)
   cTTGTAAGGACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCAtt                                                                        <  1:848762/67‑1 (MQ=255)
      gTAAGGACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTtaa                                                                 >  1:450382/1‑71 (MQ=255)
       tAAGGACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTtaa                                                                 <  1:921862/70‑1 (MQ=255)
        aaGGACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTtaat                                                                <  1:3318810/70‑1 (MQ=255)
         aGGACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTtaataa                                                              <  1:2593480/71‑1 (MQ=255)
          ggACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAg                                                             <  1:78859/71‑1 (MQ=255)
           gACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGt                                                            <  1:1057921/71‑1 (MQ=255)
           gACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGt                                                            <  1:707929/71‑1 (MQ=255)
            aCTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGt                                                            <  1:707223/70‑1 (MQ=255)
              tAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTtaa                                                                 >  1:3075837/1‑63 (MQ=255)
                attattTTTGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTtaata                                                               >  1:106744/1‑63 (MQ=255)
                attattTTTGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑Tg                                                       <  1:2119564/70‑1 (MQ=255)
                 ttattTTTGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGt                                                                    >  1:477513/1‑57 (MQ=255)
                 ttattTTTGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGa                                                     <  1:1232140/71‑1 (MQ=255)
                 ttattTTTGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGa                                                     <  1:2225942/71‑1 (MQ=255)
                  tattTTTGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGAt                                                    >  1:418124/1‑71 (MQ=255)
                  tattTTTGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGAt                                                    >  1:85200/1‑71 (MQ=255)
                  tattTTTGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGAt                                                    >  1:2182597/1‑71 (MQ=255)
                   attTTTGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGAt                                                    >  1:2254309/1‑70 (MQ=255)
                    tttttGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATg                                                   <  1:3533469/70‑1 (MQ=255)
                      tttGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAg                                                 <  1:2502571/70‑1 (MQ=255)
                        tGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAgtg                                               <  1:2389877/70‑1 (MQ=255)
                         gCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAgtgt                                              >  1:1360047/1‑70 (MQ=255)
                           atatTACTTCATTAGCTTCGATTATTTTT‑CTTTAATATTCATTATGTTAATAAGtt                                                           >  1:1502246/1‑56 (MQ=255)
                           atatTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTAct                                           <  1:2694549/71‑1 (MQ=255)
                             atTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTtaata                                                               <  1:752619/50‑1 (MQ=255)
                              ttACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGctct                                         <  1:175902/70‑1 (MQ=255)
                                aCTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCa                                       <  1:3027968/70‑1 (MQ=255)
                                aCTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCa                                       <  1:1985376/70‑1 (MQ=255)
                                 cTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTtaataa                                                              >  1:1194867/1‑47 (MQ=255)
                                 cTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCAtt                                     <  1:2485622/71‑1 (MQ=255)
                                    cATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGa                                                     >  1:1637292/1‑52 (MQ=255)
                                    cATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTg                                    <  1:2992931/69‑1 (MQ=255)
                                    cATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGa                                  >  1:2008979/1‑71 (MQ=255)
                                     aTTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGGGTGCTCTCATTGGAt                                 <  1:2791507/71‑1 (MQ=255)
                                      ttAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAg                                                             <  1:2789876/43‑1 (MQ=255)
                                      ttAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑Tg                                                       <  1:1884285/48‑1 (MQ=255)
                                      ttAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑Tg                                                       <  1:909931/48‑1 (MQ=255)
                                        aGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGa                                                     >  1:2105731/1‑48 (MQ=255)
                                          cTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGAtttttt                            <  1:1809107/71‑1 (MQ=255)
                                          cTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGAtttttt                            <  1:1410050/71‑1 (MQ=255)
                                          cTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGAtttttt                            <  1:51191/71‑1 (MQ=255)
                                            tCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAgt                                                >  1:1241951/1‑49 (MQ=255)
                                            tCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAgt                                                >  1:2625243/1‑49 (MQ=255)
                                            gtgatgatTTTTCCTTTAATATCCAGCATGTTAAGAAGTTC‑t                                                        >  1:1733264/3‑42 (MQ=12)
                                            gtgatgatTTTTCCTTTAATATCCAGCATGTTAAGAAGTTC‑Tg                                                       >  1:2571275/3‑43 (MQ=16)
                                             cGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATc                         >  1:466854/1‑71 (MQ=255)
                                              gatgatTTTTCCTTTAATATCCAGCATGTAAAGAAGTTc                                                          >  1:2962167/1‑39 (MQ=9)
                                               atgatTTTT‑CTTTAATATTCATTATGTTAATAAGtt                                                           <  1:1363014/36‑1 (MQ=255)
                                               atgatTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCg                       <  1:1035998/71‑1 (MQ=255)
                                                tgatTTTTCCTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATcc                        >  1:602030/1‑71 (MQ=255)
                                                 gatTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTc                     >  1:334079/1‑71 (MQ=255)
                                                  atTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTc                     <  1:833991/70‑1 (MQ=255)
                                                       tccTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTc                     >  1:1663272/3‑67 (MQ=255)
                                                       tccTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTg                  <  1:1699436/68‑1 (MQ=255)
                                                        ccTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTga                 <  1:2002593/69‑2 (MQ=255)
                                                         cTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTACCCGTCTTGATc               <  1:681628/71‑1 (MQ=255)
                                                         atttAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTGATc               >  1:1204721/2‑71 (MQ=255)
                                                           ttAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTGATCTg             >  1:1385251/1‑71 (MQ=255)
                                                            tAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTGATCTgt            >  1:3859165/1‑70 (MQ=255)
                                                               tatTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGAt                                 <  1:2954658/46‑1 (MQ=255)
                                                               tatTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTAACCGTCTTGTTCTGCCGc         <  1:1658843/70‑1 (MQ=255)
                                                                    aTTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGAt    <  1:3677991/70‑1 (MQ=255)
                                                                    aTTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGAt    <  1:2701468/70‑1 (MQ=255)
                                                                     ttATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATga  <  1:2009661/71‑1 (MQ=255)
                                                                      tATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTc               >  1:779794/1‑57 (MQ=255)
                                                                      tATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGa     >  1:2418661/1‑67 (MQ=255)
                                                                      |                                                                      
CTCCTTGTAAGGACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGA  >  minE/2571261‑2571399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑