Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,571,352 G→C 21.2% intergenic (+120/‑1659) yibH → / → ysaB hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,571,3520GC21.2% 94.8 / 18.9 47intergenic (+120/‑1659)yibH/ysaBhypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (11/26);  new base C (5/5);  total (16/31)
Fisher's exact test for biased strand distribution p-value = 2.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCG  >  minE/2571283‑2571420
                                                                       |                                                                    
tttGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAg                                                                      <  1:2502571/70‑1 (MQ=255)
  tGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAgtg                                                                    <  1:2389877/70‑1 (MQ=255)
   gCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAgtgt                                                                   >  1:1360047/1‑70 (MQ=255)
     atatTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTAct                                                                <  1:2694549/71‑1 (MQ=255)
        ttACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGctct                                                              <  1:175902/70‑1 (MQ=255)
          aCTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCa                                                            <  1:3027968/70‑1 (MQ=255)
          aCTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCa                                                            <  1:1985376/70‑1 (MQ=255)
           cTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCAtt                                                          <  1:2485622/71‑1 (MQ=255)
              cATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTg                                                         <  1:2992931/69‑1 (MQ=255)
              cATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGa                                                       >  1:2008979/1‑71 (MQ=255)
               aTTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGGGTGCTCTCATTGGAt                                                      <  1:2791507/71‑1 (MQ=255)
                    cTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGAtttttt                                                 <  1:51191/71‑1 (MQ=255)
                    cTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGAtttttt                                                 <  1:1410050/71‑1 (MQ=255)
                    cTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGAtttttt                                                 <  1:1809107/71‑1 (MQ=255)
                      tCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAgt                                                                     >  1:2625243/1‑49 (MQ=255)
                      tCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAgt                                                                     >  1:1241951/1‑49 (MQ=255)
                       cGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATc                                              >  1:466854/1‑71 (MQ=255)
                         atgatTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCg                                            <  1:1035998/71‑1 (MQ=255)
                          tgatTTTTCCTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATcc                                             >  1:602030/1‑71 (MQ=255)
                           gatTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTc                                          >  1:334079/1‑71 (MQ=255)
                            atTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTc                                          <  1:833991/70‑1 (MQ=255)
                                 tccTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTc                                          >  1:1663272/3‑67 (MQ=255)
                                 tccTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTg                                       <  1:1699436/68‑1 (MQ=255)
                                  ccTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTga                                      <  1:2002593/69‑2 (MQ=255)
                                   cTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTACCCGTCTTGATc                                    <  1:681628/71‑1 (MQ=255)
                                   atttAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTGATc                                    >  1:1204721/2‑71 (MQ=255)
                                     ttAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTGATCTg                                  >  1:1385251/1‑71 (MQ=255)
                                      tAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTGATCTgt                                 >  1:3859165/1‑70 (MQ=255)
                                         tatTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGAt                                                      <  1:2954658/46‑1 (MQ=255)
                                         tatTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTAACCGTCTTGTTCTGCCGc                              <  1:1658843/70‑1 (MQ=255)
                                              aTTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGAt                         <  1:2701468/70‑1 (MQ=255)
                                              aTTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGAt                         <  1:3677991/70‑1 (MQ=255)
                                               ttATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATga                       <  1:2009661/71‑1 (MQ=255)
                                               gcaTGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTa                                                <  1:3193494/45‑1 (MQ=255)
                                               gcaTGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTa                                                <  1:3041316/45‑1 (MQ=255)
                                                tATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTc                                    >  1:779794/1‑57 (MQ=255)
                                                tATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGa                          >  1:2418661/1‑67 (MQ=255)
                                                 aTGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATgag                      <  1:3209317/70‑1 (MQ=255)
                                                  tGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGAc                    >  1:355609/1‑71 (MQ=255)
                                                    ttAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACt                   <  1:3269259/70‑1 (MQ=255)
                                                    ttAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACt                   <  1:209001/70‑1 (MQ=255)
                                                      aataaGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACtat                 <  1:257301/70‑1 (MQ=255)
                                                       ataaGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACtata                <  1:2815788/70‑1 (MQ=255)
                                                        taaGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACa              >  1:13632/1‑71 (MQ=255)
                                                         aaGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACAt             >  1:3522116/1‑71 (MQ=255)
                                                           gTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACAtcg           <  1:1944225/71‑1 (MQ=255)
                                                                tGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGa                          >  1:506791/1‑52 (MQ=255)
                                                                  gATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATga                       <  1:3675681/53‑1 (MQ=255)
                                                                  gATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACtat                 >  1:3271316/1‑59 (MQ=255)
                                                                  gATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCgag      <  1:2084695/70‑1 (MQ=255)
                                                                  gATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCga       <  1:3808151/69‑1 (MQ=255)
                                                                     gAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCg  >  1:869814/1‑71 (MQ=255)
                                                                       |                                                                    
TTTGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCG  >  minE/2571283‑2571420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑