Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 385,210 G→T 9.9% T140T (ACG→ACT) ‡ cstA → carbon starvation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE385,2100GT 9.9% 190.9 / 5.2 60T140T (ACG→ACT) ‡cstAcarbon starvation protein
Reads supporting (aligned to +/- strand):  ref base G (23/31);  new base T (3/3);  total (26/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01

CTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGC  >  minE/385144‑385278
                                                                  |                                                                     
cTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGcc                                                                   >  1:1292221/1‑71 (MQ=255)
   gctgctCGCTGGGGGGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTg                                                                <  1:1496482/71‑1 (MQ=255)
    ctgctgGCGGGGGTCGTGCTGGCCGGTGCGGTTCAGGACTTTATGGTGCTGTTTATCTCCTCTCGCCGtaa                                                               <  1:1499100/71‑3 (MQ=255)
    ctgctCGCTGGGGTGGTTCTCGCCGGGGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGa                                                               <  1:541963/71‑1 (MQ=255)
          gCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGtc                                                          <  1:1018140/70‑1 (MQ=255)
          gCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGtc                                                          >  1:1387997/1‑70 (MQ=255)
              gggTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGtcgc‑tc                                                     <  1:3735801/70‑1 (MQ=255)
                gtggtTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTcgc‑tcgct                                                  <  1:2587752/71‑1 (MQ=255)
                    ttCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGt                                                           <  1:1524803/59‑1 (MQ=255)
                    ttCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTg                                                 <  1:2357919/68‑1 (MQ=255)
                    ttCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGt                                              <  1:1984576/71‑1 (MQ=255)
                      ctcGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTggg                                               >  1:223380/1‑68 (MQ=255)
                      ctcGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGt                                              >  1:2163028/1‑69 (MQ=255)
                      ctcGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGt                                              >  1:1782952/1‑69 (MQ=255)
                      ctcGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGt                                              >  1:1024244/1‑69 (MQ=255)
                      ctcGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTg                                             <  1:1044657/70‑1 (MQ=255)
                         gCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGa                                            <  1:1268447/68‑1 (MQ=255)
                         gCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGa                                            <  1:1010090/68‑1 (MQ=255)
                         gCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGa                                            <  1:3016120/68‑1 (MQ=255)
                          ccGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTg                                        >  1:1857168/1‑71 (MQ=255)
                           cggtgcggtgCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTgg                                       <  1:3503223/71‑1 (MQ=255)
                                  gtgcAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGtcgc‑tc                                                     <  1:73588/50‑1 (MQ=255)
                                    gcAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAaag                                 >  1:3469037/1‑68 (MQ=255)
                                     cAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAagaaga                             <  1:3604802/71‑1 (MQ=255)
                                     cAGGACTTTATGGTGCTGTTTATCTCCTCTCGCCGTAATGG‑CGCATCTCTTGGTGAGATGATCAaagaa                               >  1:2566236/1‑69 (MQ=255)
                                         aTTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATg                         >  1:1667077/1‑71 (MQ=255)
                                          tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGTGCTGGTCAAAGAAGAGATgg                        >  1:2983340/1‑71 (MQ=255)
                                          tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                        <  1:3428842/71‑1 (MQ=255)
                                          tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                        <  1:2382705/71‑1 (MQ=255)
                                          tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                        <  1:150903/71‑1 (MQ=255)
                                          tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                        <  1:1753496/71‑1 (MQ=255)
                                             taTGGTGCTGTTTATCTCCTCTCGCCGTAATGG‑CGCATCTCTTGGTGAGATGATCAAAGAAGAGATgggac                     >  1:1700810/2‑69 (MQ=255)
                                             taTGGTGCTGTTTATCTCCTCTCGCCGTAATGG‑CGCATCTCTTGGTGAGATGATCAAAGAAGAGATgggac                     >  1:3188782/2‑69 (MQ=255)
                                             taTGGTGCTGTTTATCTCCTCTCGCCGTAATGG‑CGCATCTCTTGGTGAGATGATCAAAGAAGAGATggg                       <  1:122186/68‑1 (MQ=255)
                                             cATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGc                     >  1:724233/1‑71 (MQ=255)
                                               tGGTGCTGTTTATCTCCTCTCGCCGTAATGG‑CGCATCTCTTGGTGAGATGATCAAAGAAGAGATGGGAcc                    <  1:766422/70‑1 (MQ=255)
                                                  tGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                        <  1:2645659/63‑1 (MQ=255)
                                                  tGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATg                         <  1:38885/62‑1 (MQ=255)
                                                    cTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCTCTGGGTGAGCTGGTCAAAGAAGAGATg                         <  1:3191507/60‑1 (MQ=255)
                                                    cTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATg                         <  1:3880871/60‑1 (MQ=255)
                                                    cTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAAccgc              >  1:1328731/1‑71 (MQ=255)
                                                     tgtttgttTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAagaaga                             >  1:3225604/1‑55 (MQ=255)
                                                      gtttgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAAccgccg            >  1:2727909/1‑71 (MQ=255)
                                                       tttgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAgaga                           >  1:737161/1‑55 (MQ=255)
                                                       tttgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                        <  1:2236217/58‑1 (MQ=255)
                                                       tttgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAAccgcc             <  1:3240845/69‑1 (MQ=255)
                                                       tttgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCgg           <  1:1378872/71‑1 (MQ=255)
                                                         tgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATggg                       >  1:2850384/1‑57 (MQ=255)
                                                         tgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATggg                       >  1:3492533/1‑57 (MQ=255)
                                                         tgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCgggg         >  1:1070097/1‑71 (MQ=255)
                                                         tgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCgggg         >  1:3356221/1‑71 (MQ=255)
                                                           tttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGt        <  1:3695223/70‑1 (MQ=255)
                                                             tCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGAt                          <  1:729648/50‑1 (MQ=255)
                                                             tCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCa                   >  1:2277568/1‑57 (MQ=255)
                                                             tCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGa      <  1:2329850/70‑1 (MQ=255)
                                                              cTACGCGCCGTGACGGTCGC‑TCGCTTGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGAtt    >  1:1979833/1‑71 (MQ=255)
                                                              cTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGAtt    >  1:1375815/1‑71 (MQ=255)
                                                              cTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGAtt    >  1:2331255/1‑71 (MQ=255)
                                                              cTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGAt     <  1:3189982/70‑1 (MQ=255)
                                                                aCGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATg                         <  1:851634/48‑1 (MQ=255)
                                                                 cgcgCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTgc  <  1:548553/70‑1 (MQ=255)
                                                                 cgcgCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTgc  <  1:1671666/70‑1 (MQ=255)
                                                                  |                                                                     
CTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGC  >  minE/385144‑385278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑