Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 385,231 G→T 9.6% L147L (CTG→CTT)  cstA → carbon starvation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE385,2310GT 9.6% 197.4 / 4.7 62L147L (CTG→CTT) cstAcarbon starvation protein
Reads supporting (aligned to +/- strand):  ref base G (25/31);  new base T (4/2);  total (29/33)
Fisher's exact test for biased strand distribution p-value = 4.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCTTTATGA  >  minE/385164‑385298
                                                                    |                                                                   
ttCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTg                                                                     <  1:2357919/68‑1 (MQ=255)
ttCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGt                                                                  <  1:1984576/71‑1 (MQ=255)
  ctcGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTggg                                                                   >  1:223380/1‑68 (MQ=255)
  ctcGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGt                                                                  >  1:1024244/1‑69 (MQ=255)
  ctcGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGt                                                                  >  1:1782952/1‑69 (MQ=255)
  ctcGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGt                                                                  >  1:2163028/1‑69 (MQ=255)
  ctcGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTg                                                                 <  1:1044657/70‑1 (MQ=255)
     gCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGa                                                                <  1:1268447/68‑1 (MQ=255)
     gCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGa                                                                <  1:3016120/68‑1 (MQ=255)
     gCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGa                                                                <  1:1010090/68‑1 (MQ=255)
      ccGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTg                                                            >  1:1857168/1‑71 (MQ=255)
       cggtgcggtgCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTgg                                                           <  1:3503223/71‑1 (MQ=255)
                gcAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAaag                                                     >  1:3469037/1‑68 (MQ=255)
                 cAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAagaaga                                                 <  1:3604802/71‑1 (MQ=255)
                 cAGGACTTTATGGTGCTGTTTATCTCCTCTCGCCGTAATGG‑CGCATCTCTTGGTGAGATGATCAaagaa                                                   >  1:2566236/1‑69 (MQ=255)
                     aTTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATg                                             >  1:1667077/1‑71 (MQ=255)
                      tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGTGCTGGTCAAAGAAGAGATgg                                            >  1:2983340/1‑71 (MQ=255)
                      tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                                            <  1:3428842/71‑1 (MQ=255)
                      tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                                            <  1:2382705/71‑1 (MQ=255)
                      tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                                            <  1:1753496/71‑1 (MQ=255)
                      tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                                            <  1:150903/71‑1 (MQ=255)
                         taTGGTGCTGTTTATCTCCTCTCGCCGTAATGG‑CGCATCTCTTGGTGAGATGATCAAAGAAGAGATgggac                                         >  1:3188782/2‑69 (MQ=255)
                         taTGGTGCTGTTTATCTCCTCTCGCCGTAATGG‑CGCATCTCTTGGTGAGATGATCAAAGAAGAGATgggac                                         >  1:1700810/2‑69 (MQ=255)
                         taTGGTGCTGTTTATCTCCTCTCGCCGTAATGG‑CGCATCTCTTGGTGAGATGATCAAAGAAGAGATggg                                           <  1:122186/68‑1 (MQ=255)
                         cATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGc                                         >  1:724233/1‑71 (MQ=255)
                           tGGTGCTGTTTATCTCCTCTCGCCGTAATGG‑CGCATCTCTTGGTGAGATGATCAAAGAAGAGATGGGAcc                                        <  1:766422/70‑1 (MQ=255)
                              tGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                                            <  1:2645659/63‑1 (MQ=255)
                              tGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATg                                             <  1:38885/62‑1 (MQ=255)
                                cTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCTCTGGGTGAGCTGGTCAAAGAAGAGATg                                             <  1:3191507/60‑1 (MQ=255)
                                cTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATg                                             <  1:3880871/60‑1 (MQ=255)
                                cTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAAccgc                                  >  1:1328731/1‑71 (MQ=255)
                                 tgtttgttTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAagaaga                                                 >  1:3225604/1‑55 (MQ=255)
                                  gtttgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAAccgccg                                >  1:2727909/1‑71 (MQ=255)
                                   tttgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAgaga                                               >  1:737161/1‑55 (MQ=255)
                                   tttgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                                            <  1:2236217/58‑1 (MQ=255)
                                   tttgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAAccgcc                                 <  1:3240845/69‑1 (MQ=255)
                                   tttgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCgg                               <  1:1378872/71‑1 (MQ=255)
                                     tgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATggg                                           >  1:3492533/1‑57 (MQ=255)
                                     tgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATggg                                           >  1:2850384/1‑57 (MQ=255)
                                     tgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCgggg                             >  1:3356221/1‑71 (MQ=255)
                                     tgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCgggg                             >  1:1070097/1‑71 (MQ=255)
                                       tttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGt                            <  1:3695223/70‑1 (MQ=255)
                                         tCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGAt                                              <  1:729648/50‑1 (MQ=255)
                                         tCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCa                                       >  1:2277568/1‑57 (MQ=255)
                                         tCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGa                          <  1:2329850/70‑1 (MQ=255)
                                          cTACGCGCCGTGACGGTCGC‑TCGCTTGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGAtt                        >  1:1979833/1‑71 (MQ=255)
                                          cTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGAtt                        >  1:1375815/1‑71 (MQ=255)
                                          cTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGAtt                        >  1:2331255/1‑71 (MQ=255)
                                          cTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGAt                         <  1:3189982/70‑1 (MQ=255)
                                            aCGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATg                                             <  1:851634/48‑1 (MQ=255)
                                             cgcgCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTgc                      <  1:548553/70‑1 (MQ=255)
                                             cgcgCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTgc                      <  1:1671666/70‑1 (MQ=255)
                                                 ccGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCtgg                 <  1:1977237/71‑1 (MQ=255)
                                                     gACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAgaga                                               >  1:2444047/1‑37 (MQ=255)
                                                     gACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGAt                                              <  1:1411758/38‑1 (MQ=255)
                                                      aCGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGcc            >  1:383128/1‑71 (MQ=255)
                                                          tcgc‑tcgcTGGGTGAGCTGGTCAAAGAAGAGATgggg                                          >  1:3600125/1‑37 (MQ=255)
                                                          tcgc‑tcgcTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCt        >  1:1313037/1‑71 (MQ=255)
                                                          tcgc‑tcgcTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCt        <  1:2502337/71‑1 (MQ=255)
                                                            gc‑tcgctGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCttt      <  1:1056735/71‑1 (MQ=255)
                                                            gc‑tcgctGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCttt      <  1:1779906/71‑1 (MQ=255)
                                                             c‑tcgctGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGAtt                        <  1:1563325/52‑1 (MQ=255)
                                                                 gctGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGAt                         >  1:490806/1‑48 (MQ=255)
                                                                 gctGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGTTGGCCTGCTTTATGa  >  1:2309060/1‑71 (MQ=255)
                                                                    |                                                                   
TTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCTTTATGA  >  minE/385164‑385298

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑