Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	608,676	A→G	6.8%	G526G (GGT→GGC)	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	608,676	0	A	G	6.8%	249.5 / 7.4	88	G526G (GGT→GGC)	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand):  ref base A (42/40);  new base G (2/4);  total (44/44)
Fisher's exact test for biased strand distribution p-value = 6.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.99e-01

ACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGT  >  minE/608607‑608746                                                                      |                                                                        aCGAATCGTTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATAc                                                                        >  1:2262224/1‑71 (MQ=255) aCGAATCGTTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATAc                                                                        >  1:2257497/1‑71 (MQ=255)       cGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCacac                                                                   <  1:2421784/70‑1 (MQ=255)        ggTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACcac                                                                     >  1:2081700/1‑67 (MQ=255)        ggTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCacac                                                                   <  1:2426413/69‑1 (MQ=255)        ggTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGc                                                                 >  1:2124819/1‑71 (MQ=255)          tttAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCa                                                               <  1:2145518/71‑1 (MQ=255)           ttAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCAt                                                              >  1:3191784/1‑71 (MQ=255)           ttAACTTTCGCATATTTGATTGCAGACA‑GGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATg                                                             >  1:3265956/1‑71 (MQ=255)            tAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATg                                                             >  1:3395898/1‑71 (MQ=255)            tAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATg                                                             >  1:1896349/1‑71 (MQ=255)            tAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATg                                                             >  1:3740986/1‑71 (MQ=255)              aCTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGt                                                           <  1:1033046/71‑1 (MQ=255)              aCTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGt                                                           <  1:2688667/71‑1 (MQ=255)              aCTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGt                                                           >  1:2796511/1‑71 (MQ=255)              aCTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGt                                                           <  1:1313648/71‑1 (MQ=255)              aCTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGt                                                           <  1:1340474/71‑1 (MQ=255)               cTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATa                                                                         >  1:242166/1‑56 (MQ=255)               cTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTg                                                          <  1:3548789/71‑1 (MQ=255)               cTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTg                                                          <  1:1379505/71‑1 (MQ=255)                  tCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGt                                                           >  1:335214/1‑67 (MQ=255)                  gggCATATTTGATGGCAGACAGGGAGTCCGTCGCCACCGACAGGCCCGCGATGCCGCATGCCATAGTGCga                                                       <  1:97846/69‑2 (MQ=255)                     aatatTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTg                                                          <  1:409848/64‑1 (MQ=255)                      atatTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGt                                                           <  1:1796937/63‑1 (MQ=255)                      atatTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGt                                                           <  1:1801504/63‑1 (MQ=255)                      atatTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCg                                                        >  1:3663084/1‑66 (MQ=255)                      atatTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCg                                                        <  1:1283174/66‑1 (MQ=255)                       tatTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATAcc                                                                       <  1:1631657/50‑1 (MQ=255)                        atTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTg                                                          <  1:3112556/62‑1 (MQ=255)                        atTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGc                                                         >  1:2808678/1‑63 (MQ=255)                         tttGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGAGGATa‑a                                                  >  1:131469/1‑68 (MQ=255)                          ttGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACg                                                <  1:549217/69‑1 (MQ=255)                            gATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATa                                                                         <  1:809871/43‑1 (MQ=255)                            gATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATa                                                                         <  1:3543919/43‑1 (MQ=255)                            gATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATa                                                                         <  1:3795398/43‑1 (MQ=255)                            gATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATa‑a                                                  >  1:3247054/1‑65 (MQ=255)                            gATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCAc                                            >  1:880896/1‑71 (MQ=255)                             aTTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACg                                           <  1:1839862/71‑1 (MQ=255)                                gCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGa                                                      >  1:517001/1‑58 (MQ=255)                                gCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGt                                         <  1:882219/70‑1 (MQ=255)                                gCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTc                                        >  1:3093960/1‑71 (MQ=255)                                   gACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTg                                     >  1:989717/1‑71 (MQ=255)                                    aCAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGt                                               >  1:3476678/1‑60 (MQ=255)                                    aCAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTg                                     >  1:1112320/1‑70 (MQ=255)                                    aCAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTg                                     >  1:3782036/1‑70 (MQ=255)                                    aCAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTg                                     >  1:827740/1‑70 (MQ=255)                                        ggAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAgcg                                <  1:3705642/71‑1 (MQ=255)                                        ggAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAgcg                                >  1:2005090/1‑71 (MQ=255)                                         gAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCg                                                        <  1:3402155/47‑1 (MQ=255)                                          aGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATa                                                    <  1:1920507/50‑1 (MQ=255)                                           gTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCa                             <  1:3249682/71‑1 (MQ=255)                                           gTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCa                             <  1:2950475/71‑1 (MQ=255)                                            tCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑Ac                                                 >  1:3078752/1‑50 (MQ=255)                                            tCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑Ac                                                 >  1:3074245/1‑50 (MQ=255)                                            tCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCa                             <  1:1277/70‑1 (MQ=255)                                            tCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCAt                            >  1:3428425/1‑71 (MQ=255)                                            tCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCAt                            <  1:609911/71‑1 (MQ=255)                                              agcagcAATGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCa                          <  1:98677/71‑1 (MQ=255)                                              agcagcAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑Ac                                                 <  1:2761886/48‑1 (MQ=255)                                              agcagcAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCa                          <  1:2589152/71‑1 (MQ=255)                                                cagcaACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAgcg                                <  1:3737942/63‑1 (MQ=255)                                                cagcaACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCaga                        <  1:1218174/71‑1 (MQ=255)                                                 agcaACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGcc                              <  1:895464/64‑1 (MQ=255)                                                 agcaACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCagag                       <  1:3678985/71‑1 (MQ=255)                                                  gcaACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCa                          >  1:220826/1‑67 (MQ=255)                                                    aaCGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCa                                   <  1:2887690/56‑1 (MQ=255)                                                      ccgACAGGCCCGCGATGCCGCATGCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCt                  <  1:1898037/69‑1 (MQ=255)                                                      cGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCt                  >  1:3421578/1‑71 (MQ=255)                                                       ggACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCag                         >  1:1146727/1‑63 (MQ=255)                                                       cgACAGGCCCGCGATGCCGCATGCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCtt                 >  1:150386/2‑71 (MQ=255)                                                        gACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTg                                     >  1:1831780/1‑50 (MQ=255)                                                         aCAGGCCCGCGATGCCGCATGCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCg               >  1:943171/1‑71 (MQ=255)                                                         aCAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCg               >  1:1107471/1‑71 (MQ=255)                                                         aCAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCg               >  1:3799173/1‑71 (MQ=255)                                                         aCAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCg               >  1:3372418/1‑71 (MQ=255)                                                          cAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCg               >  1:3149032/1‑70 (MQ=255)                                                          cAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGt              <  1:3694058/71‑1 (MQ=255)                                                           aGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCg               >  1:96270/1‑69 (MQ=255)                                                            gACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCGGAGAGGCTTCGTa             <  1:215811/70‑1 (MQ=255)                                                            gACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTa             >  1:3193277/1‑70 (MQ=255)                                                             aCCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGc           <  1:1685785/71‑1 (MQ=255)                                                               cAGCGATACCACACGCCATGGTGCGGATA‑ACGTCa                                             >  1:25645/1‑35 (MQ=255)                                                               cAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACgg                                          <  1:2526365/38‑1 (MQ=255)                                                               cAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCa                                   <  1:2533402/45‑1 (MQ=255)                                                                 gCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGt        >  1:2710663/1‑70 (MQ=255)                                                                 gCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTa       >  1:1630226/1‑71 (MQ=255)                                                                 gCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTa       >  1:194067/1‑71 (MQ=255)                                                                  cGATGCCGCATGCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTAc      <  1:2629928/71‑1 (MQ=255)                                                                   gATGCCGCATGCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTAc      <  1:3079912/70‑1 (MQ=255)                                                                    aTACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTg         <  1:1217645/66‑1 (MQ=255)                                                                    aTACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACtt    <  1:1694886/71‑1 (MQ=255)                                                                     tACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTg   >  1:1711142/1‑71 (MQ=255)                                                                      aCCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGt  >  1:1712925/1‑71 (MQ=255)                                                                      aCCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGt  >  1:411857/1‑71 (MQ=255)                                                                      |                                                                        ACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGT  >  minE/608607‑608746

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑