Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	608,682	C→T	6.9%	A524A (GCG→GCA)	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	608,682	0	C	T	6.9%	263.6 / 6.2	86	A524A (GCG→GCA)	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand):  ref base C (41/39);  new base T (2/4);  total (43/43)
Fisher's exact test for biased strand distribution p-value = 6.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

CGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCG  >  minE/608613‑608748                                                                      |                                                                    cGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCacac                                                                     <  1:2421784/70‑1 (MQ=255)  ggTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCacac                                                                     <  1:2426413/69‑1 (MQ=255)  ggTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGc                                                                   >  1:2124819/1‑71 (MQ=255)    tttAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCa                                                                 <  1:2145518/71‑1 (MQ=255)     ttAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCAt                                                                >  1:3191784/1‑71 (MQ=255)     ttAACTTTCGCATATTTGATTGCAGACA‑GGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATg                                                               >  1:3265956/1‑71 (MQ=255)      tAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATg                                                               >  1:1896349/1‑71 (MQ=255)      tAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATg                                                               >  1:3395898/1‑71 (MQ=255)      tAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATg                                                               >  1:3740986/1‑71 (MQ=255)        aCTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGt                                                             <  1:1033046/71‑1 (MQ=255)        aCTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGt                                                             <  1:2688667/71‑1 (MQ=255)        aCTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGt                                                             >  1:2796511/1‑71 (MQ=255)        aCTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGt                                                             <  1:1340474/71‑1 (MQ=255)        aCTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGt                                                             <  1:1313648/71‑1 (MQ=255)         cTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTg                                                            <  1:1379505/71‑1 (MQ=255)         cTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTg                                                            <  1:3548789/71‑1 (MQ=255)            tCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGt                                                             >  1:335214/1‑67 (MQ=255)            gggCATATTTGATGGCAGACAGGGAGTCCGTCGCCACCGACAGGCCCGCGATGCCGCATGCCATAGTGCga                                                         <  1:97846/69‑2 (MQ=255)               aatatTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTg                                                            <  1:409848/64‑1 (MQ=255)                atatTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGt                                                             <  1:1801504/63‑1 (MQ=255)                atatTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGt                                                             <  1:1796937/63‑1 (MQ=255)                atatTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCg                                                          <  1:1283174/66‑1 (MQ=255)                atatTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCg                                                          >  1:3663084/1‑66 (MQ=255)                  atTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTg                                                            <  1:3112556/62‑1 (MQ=255)                  atTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGc                                                           >  1:2808678/1‑63 (MQ=255)                   tttGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGAGGATa‑a                                                    >  1:131469/1‑68 (MQ=255)                    ttGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACg                                                  <  1:549217/69‑1 (MQ=255)                      gATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATa‑a                                                    >  1:3247054/1‑65 (MQ=255)                      gATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCAc                                              >  1:880896/1‑71 (MQ=255)                       aTTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACg                                             <  1:1839862/71‑1 (MQ=255)                          gCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGa                                                        >  1:517001/1‑58 (MQ=255)                          gCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGt                                           <  1:882219/70‑1 (MQ=255)                          gCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTc                                          >  1:3093960/1‑71 (MQ=255)                             gACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTg                                       >  1:989717/1‑71 (MQ=255)                              aCAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGt                                                 >  1:3476678/1‑60 (MQ=255)                              aCAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTg                                       >  1:3782036/1‑70 (MQ=255)                              aCAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTg                                       >  1:1112320/1‑70 (MQ=255)                              aCAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTg                                       >  1:827740/1‑70 (MQ=255)                                  ggAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAgcg                                  >  1:2005090/1‑71 (MQ=255)                                  ggAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAgcg                                  <  1:3705642/71‑1 (MQ=255)                                   gAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCg                                                          <  1:3402155/47‑1 (MQ=255)                                    aGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATa                                                      <  1:1920507/50‑1 (MQ=255)                                     gTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCa                               <  1:2950475/71‑1 (MQ=255)                                     gTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCa                               <  1:3249682/71‑1 (MQ=255)                                      tCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑Ac                                                   >  1:3074245/1‑50 (MQ=255)                                      tCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑Ac                                                   >  1:3078752/1‑50 (MQ=255)                                      tCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCa                               <  1:1277/70‑1 (MQ=255)                                      tCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCAt                              >  1:3428425/1‑71 (MQ=255)                                      tCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCAt                              <  1:609911/71‑1 (MQ=255)                                        agcagcAATGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCa                            <  1:98677/71‑1 (MQ=255)                                        agcagcAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑Ac                                                   <  1:2761886/48‑1 (MQ=255)                                        agcagcAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCa                            <  1:2589152/71‑1 (MQ=255)                                          cagcaACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAgcg                                  <  1:3737942/63‑1 (MQ=255)                                          cagcaACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCaga                          <  1:1218174/71‑1 (MQ=255)                                           agcaACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGcc                                <  1:895464/64‑1 (MQ=255)                                           agcaACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCagag                         <  1:3678985/71‑1 (MQ=255)                                            gcaACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCa                            >  1:220826/1‑67 (MQ=255)                                              aaCGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCa                                     <  1:2887690/56‑1 (MQ=255)                                                ccgACAGGCCCGCGATGCCGCATGCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCt                    <  1:1898037/69‑1 (MQ=255)                                                cGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCt                    >  1:3421578/1‑71 (MQ=255)                                                 ggACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCag                           >  1:1146727/1‑63 (MQ=255)                                                 cgACAGGCCCGCGATGCCGCATGCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCtt                   >  1:150386/2‑71 (MQ=255)                                                  gACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTg                                       >  1:1831780/1‑50 (MQ=255)                                                   aCAGGCCCGCGATGCCGCATGCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCg                 >  1:943171/1‑71 (MQ=255)                                                   aCAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCg                 >  1:3799173/1‑71 (MQ=255)                                                   aCAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCg                 >  1:1107471/1‑71 (MQ=255)                                                   aCAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCg                 >  1:3372418/1‑71 (MQ=255)                                                    cAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCg                 >  1:3149032/1‑70 (MQ=255)                                                    cAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGt                <  1:3694058/71‑1 (MQ=255)                                                     aGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCg                 >  1:96270/1‑69 (MQ=255)                                                      gACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCGGAGAGGCTTCGTa               <  1:215811/70‑1 (MQ=255)                                                      gACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTa               >  1:3193277/1‑70 (MQ=255)                                                       aCCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGc             <  1:1685785/71‑1 (MQ=255)                                                         cAGCGATACCACACGCCATGGTGCGGATA‑ACGTCa                                               >  1:25645/1‑35 (MQ=255)                                                         cAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACgg                                            <  1:2526365/38‑1 (MQ=255)                                                         cAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCa                                     <  1:2533402/45‑1 (MQ=255)                                                           gCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGt          >  1:2710663/1‑70 (MQ=255)                                                           gCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTa         >  1:194067/1‑71 (MQ=255)                                                           gCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTa         >  1:1630226/1‑71 (MQ=255)                                                            cGATGCCGCATGCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTAc        <  1:2629928/71‑1 (MQ=255)                                                             gATGCCGCATGCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTAc        <  1:3079912/70‑1 (MQ=255)                                                              aTACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTg           <  1:1217645/66‑1 (MQ=255)                                                              aTACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACtt      <  1:1694886/71‑1 (MQ=255)                                                               tACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTg     >  1:1711142/1‑71 (MQ=255)                                                                aCCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGt    >  1:1712925/1‑71 (MQ=255)                                                                aCCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGt    >  1:411857/1‑71 (MQ=255)                                                                 ccACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTc   <  1:3614236/71‑1 (MQ=255)                                                                  cacaCGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCg  <  1:256898/71‑1 (MQ=255)                                                                    cacGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGt    >  1:1554954/1‑67 (MQ=255)                                                                      cGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTa         <  1:1576949/60‑1 (MQ=255)                                                                      |                                                                    CGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCG  >  minE/608613‑608748

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑