BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	608,688	G→A	10.3%	T522T (ACC→ACT)	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	608,688	0	G	A	10.3%	243.2 / 15.1	87	T522T (ACC→ACT)	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand): ref base G (37/41); new base A (4/5); total (41/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01

TTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTA  >  minE/608617‑608756

ttAACTTTCGCATATTTGATTGCAGACA‑GGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATg                                                                       >  1:3265956/1‑71 (MQ=255)

 tAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATg                                                                       >  1:3740986/1‑71 (MQ=255)

 tAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATg                                                                       >  1:3395898/1‑71 (MQ=255)

 tAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATg                                                                       >  1:1896349/1‑71 (MQ=255)

   aCTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGt                                                                     <  1:1033046/71‑1 (MQ=255)

   aCTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGt                                                                     <  1:1313648/71‑1 (MQ=255)

   aCTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGt                                                                     <  1:2688667/71‑1 (MQ=255)

   aCTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGt                                                                     <  1:1340474/71‑1 (MQ=255)

   aCTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGt                                                                     >  1:2796511/1‑71 (MQ=255)

    cTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTg                                                                    <  1:3548789/71‑1 (MQ=255)

    cTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTg                                                                    <  1:1379505/71‑1 (MQ=255)

       tCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGt                                                                     >  1:335214/1‑67 (MQ=255)

       gggCATATTTGATGGCAGACAGGGAGTCCGTCGCCACCGACAGGCCCGCGATGCCGCATGCCATAGTGCga                                                                 <  1:97846/69‑2 (MQ=255)

          aatatTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTg                                                                    <  1:409848/64‑1 (MQ=255)

           atatTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGt                                                                     <  1:1801504/63‑1 (MQ=255)

           atatTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGt                                                                     <  1:1796937/63‑1 (MQ=255)

           atatTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCg                                                                  <  1:1283174/66‑1 (MQ=255)

           atatTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCg                                                                  >  1:3663084/1‑66 (MQ=255)

             atTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTg                                                                    <  1:3112556/62‑1 (MQ=255)

             atTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGc                                                                   >  1:2808678/1‑63 (MQ=255)

              tttGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGAGGATa‑a                                                            >  1:131469/1‑68 (MQ=255)

               ttGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACg                                                          <  1:549217/69‑1 (MQ=255)

                 gATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATa‑a                                                            >  1:3247054/1‑65 (MQ=255)

                 gATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCAc                                                      >  1:880896/1‑71 (MQ=255)

                  aTTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACg                                                     <  1:1839862/71‑1 (MQ=255)

                     gCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGa                                                                >  1:517001/1‑58 (MQ=255)

                     gCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGt                                                   <  1:882219/70‑1 (MQ=255)

                     gCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTc                                                  >  1:3093960/1‑71 (MQ=255)

                        gACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTg                                               >  1:989717/1‑71 (MQ=255)

                         aCAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGt                                                         >  1:3476678/1‑60 (MQ=255)

                         aCAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTg                                               >  1:1112320/1‑70 (MQ=255)

                         aCAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTg                                               >  1:827740/1‑70 (MQ=255)

                         aCAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTg                                               >  1:3782036/1‑70 (MQ=255)

                             ggAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAgcg                                          >  1:2005090/1‑71 (MQ=255)

                             ggAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAgcg                                          <  1:3705642/71‑1 (MQ=255)

                              gAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCg                                                                  <  1:3402155/47‑1 (MQ=255)

                               aGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATa                                                              <  1:1920507/50‑1 (MQ=255)

                                gTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCa                                       <  1:2950475/71‑1 (MQ=255)

                                gTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCa                                       <  1:3249682/71‑1 (MQ=255)

                                 tCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑Ac                                                           >  1:3074245/1‑50 (MQ=255)

                                 tCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑Ac                                                           >  1:3078752/1‑50 (MQ=255)

                                 tCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCa                                       <  1:1277/70‑1 (MQ=255)

                                 tCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCAt                                      >  1:3428425/1‑71 (MQ=255)

                                 tCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCAt                                      <  1:609911/71‑1 (MQ=255)

                                   agcagcAATGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCa                                    <  1:98677/71‑1 (MQ=255)

                                   agcagcAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑Ac                                                           <  1:2761886/48‑1 (MQ=255)

                                   agcagcAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCa                                    <  1:2589152/71‑1 (MQ=255)

                                     cagcaACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAgcg                                          <  1:3737942/63‑1 (MQ=255)

                                     cagcaACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCaga                                  <  1:1218174/71‑1 (MQ=255)

                                      agcaACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGcc                                        <  1:895464/64‑1 (MQ=255)

                                      agcaACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCagag                                 <  1:3678985/71‑1 (MQ=255)

                                       gcaACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCa                                    >  1:220826/1‑67 (MQ=255)

                                         aaCGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCa                                             <  1:2887690/56‑1 (MQ=255)

                                           ccgACAGGCCCGCGATGCCGCATGCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCt                            <  1:1898037/69‑1 (MQ=255)

                                           cGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCt                            >  1:3421578/1‑71 (MQ=255)

                                            ggACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCag                                   >  1:1146727/1‑63 (MQ=255)

                                            cgACAGGCCCGCGATGCCGCATGCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCtt                           >  1:150386/2‑71 (MQ=255)

                                             gACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTg                                               >  1:1831780/1‑50 (MQ=255)

                                              aCAGGCCCGCGATGCCGCATGCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCg                         >  1:943171/1‑71 (MQ=255)

                                              aCAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCg                         >  1:1107471/1‑71 (MQ=255)

                                              aCAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCg                         >  1:3372418/1‑71 (MQ=255)

                                              aCAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCg                         >  1:3799173/1‑71 (MQ=255)

                                               cAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCg                         >  1:3149032/1‑70 (MQ=255)

                                               cAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGt                        <  1:3694058/71‑1 (MQ=255)

                                                aGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCg                         >  1:96270/1‑69 (MQ=255)

                                                 gACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCGGAGAGGCTTCGTa                       <  1:215811/70‑1 (MQ=255)

                                                 gACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTa                       >  1:3193277/1‑70 (MQ=255)

                                                  aCCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGc                     <  1:1685785/71‑1 (MQ=255)

                                                    cAGCGATACCACACGCCATGGTGCGGATA‑ACGTCa                                                       >  1:25645/1‑35 (MQ=255)

                                                    cAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACgg                                                    <  1:2526365/38‑1 (MQ=255)

                                                    cAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCa                                             <  1:2533402/45‑1 (MQ=255)

                                                      gCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGt                  >  1:2710663/1‑70 (MQ=255)

                                                      gCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTa                 >  1:194067/1‑71 (MQ=255)

                                                      gCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTa                 >  1:1630226/1‑71 (MQ=255)

                                                       cGATGCCGCATGCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTAc                <  1:2629928/71‑1 (MQ=255)

                                                        gATGCCGCATGCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTAc                <  1:3079912/70‑1 (MQ=255)

                                                         aTACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTg                   <  1:1217645/66‑1 (MQ=255)

                                                         aTACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACtt              <  1:1694886/71‑1 (MQ=255)

                                                          tACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTg             >  1:1711142/1‑71 (MQ=255)

                                                           aCCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGt            >  1:411857/1‑71 (MQ=255)

                                                           aCCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGt            >  1:1712925/1‑71 (MQ=255)

                                                            ccACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTc           <  1:3614236/71‑1 (MQ=255)

                                                             cacaCGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCg          <  1:256898/71‑1 (MQ=255)

                                                               catgCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTGTCGt         <  1:1649123/67‑1 (MQ=255)

                                                               cacGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGt            >  1:1554954/1‑67 (MQ=255)

                                                                 tgCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCa      >  1:2448498/2‑71 (MQ=255)

                                                                 tgCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCa      >  1:514557/2‑71 (MQ=255)

                                                                 cGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTa                 <  1:1576949/60‑1 (MQ=255)

                                                                     aTGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATg    >  1:3881228/1‑69 (MQ=255)

                                                                     aTGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTa  <  1:448813/71‑1 (MQ=255)

                                                                     aTGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTa  <  1:1979514/71‑1 (MQ=255)

TTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTA  >  minE/608617‑608756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`