Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	608,700	G→A	10.1%	D518D (GAC→GAT)	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	608,700	0	G	A	10.1%	224.3 / 12.8	79	D518D (GAC→GAT)	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand):  ref base G (33/38);  new base A (4/4);  total (37/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

TTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCA  >  minE/608632‑608770                                                                      |                                                                       ttGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACg                                                                        <  1:549217/69‑1 (MQ=255)   gATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCAc                                                                    >  1:880896/1‑71 (MQ=255)    aTTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACg                                                                   <  1:1839862/71‑1 (MQ=255)       gCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGt                                                                 <  1:882219/70‑1 (MQ=255)       gCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTc                                                                >  1:3093960/1‑71 (MQ=255)          gACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTg                                                             >  1:989717/1‑71 (MQ=255)           aCAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGt                                                                       >  1:3476678/1‑60 (MQ=255)           aCAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTg                                                             >  1:1112320/1‑70 (MQ=255)           aCAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTg                                                             >  1:827740/1‑70 (MQ=255)           aCAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTg                                                             >  1:3782036/1‑70 (MQ=255)               ggAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAgcg                                                        >  1:2005090/1‑71 (MQ=255)               ggAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAgcg                                                        <  1:3705642/71‑1 (MQ=255)                  gTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCa                                                     <  1:3249682/71‑1 (MQ=255)                  gTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCa                                                     <  1:2950475/71‑1 (MQ=255)                   tCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCa                                                     <  1:1277/70‑1 (MQ=255)                   tCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCAt                                                    <  1:609911/71‑1 (MQ=255)                   tCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCAt                                                    >  1:3428425/1‑71 (MQ=255)                     agcagcAATGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCa                                                  <  1:98677/71‑1 (MQ=255)                     agcagcAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCa                                                  <  1:2589152/71‑1 (MQ=255)                       cagcaACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAgcg                                                        <  1:3737942/63‑1 (MQ=255)                       cagcaACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCaga                                                <  1:1218174/71‑1 (MQ=255)                        agcaACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGcc                                                      <  1:895464/64‑1 (MQ=255)                        agcaACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCagag                                               <  1:3678985/71‑1 (MQ=255)                         gcaACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCa                                                  >  1:220826/1‑67 (MQ=255)                           aaCGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCa                                                           <  1:2887690/56‑1 (MQ=255)                             ccgACAGGCCCGCGATGCCGCATGCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCt                                          <  1:1898037/69‑1 (MQ=255)                             cGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCt                                          >  1:3421578/1‑71 (MQ=255)                              ggACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCag                                                 >  1:1146727/1‑63 (MQ=255)                              cgACAGGCCCGCGATGCCGCATGCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCtt                                         >  1:150386/2‑71 (MQ=255)                               gACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTg                                                             >  1:1831780/1‑50 (MQ=255)                                aCAGGCCCGCGATGCCGCATGCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCg                                       >  1:943171/1‑71 (MQ=255)                                aCAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCg                                       >  1:3799173/1‑71 (MQ=255)                                aCAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCg                                       >  1:3372418/1‑71 (MQ=255)                                aCAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCg                                       >  1:1107471/1‑71 (MQ=255)                                 cAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCg                                       >  1:3149032/1‑70 (MQ=255)                                 cAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGt                                      <  1:3694058/71‑1 (MQ=255)                                  aGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCg                                       >  1:96270/1‑69 (MQ=255)                                   gACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCGGAGAGGCTTCGTa                                     <  1:215811/70‑1 (MQ=255)                                   gACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTa                                     >  1:3193277/1‑70 (MQ=255)                                    aCCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGc                                   <  1:1685785/71‑1 (MQ=255)                                      cAGCGATACCACACGCCATGGTGCGGATA‑ACGTCa                                                                     >  1:25645/1‑35 (MQ=255)                                      cAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACgg                                                                  <  1:2526365/38‑1 (MQ=255)                                      cAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCa                                                           <  1:2533402/45‑1 (MQ=255)                                        gCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGt                                >  1:2710663/1‑70 (MQ=255)                                        gCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTa                               >  1:1630226/1‑71 (MQ=255)                                        gCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTa                               >  1:194067/1‑71 (MQ=255)                                         cGATGCCGCATGCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTAc                              <  1:2629928/71‑1 (MQ=255)                                          gATGCCGCATGCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTAc                              <  1:3079912/70‑1 (MQ=255)                                           aTACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTg                                 <  1:1217645/66‑1 (MQ=255)                                           aTACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACtt                            <  1:1694886/71‑1 (MQ=255)                                            tACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTg                           >  1:1711142/1‑71 (MQ=255)                                             aCCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGt                          >  1:411857/1‑71 (MQ=255)                                             aCCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGt                          >  1:1712925/1‑71 (MQ=255)                                              ccACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTc                         <  1:3614236/71‑1 (MQ=255)                                               cacaCGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCg                        <  1:256898/71‑1 (MQ=255)                                                 catgCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTGTCGt                       <  1:1649123/67‑1 (MQ=255)                                                 cacGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGt                          >  1:1554954/1‑67 (MQ=255)                                                   tgCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCa                    >  1:2448498/2‑71 (MQ=255)                                                   tgCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCa                    >  1:514557/2‑71 (MQ=255)                                                   cGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTa                               <  1:1576949/60‑1 (MQ=255)                                                       aTGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATg                  >  1:3881228/1‑69 (MQ=255)                                                       aTGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTa                <  1:1979514/71‑1 (MQ=255)                                                       aTGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTa                <  1:448813/71‑1 (MQ=255)                                                          gTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGt              <  1:1766988/70‑1 (MQ=255)                                                          gTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTg             <  1:2598374/71‑1 (MQ=255)                                                           tGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTgg            <  1:3700027/71‑1 (MQ=255)                                                           tGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTgg            <  1:693798/71‑1 (MQ=255)                                                             cGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTc                                        >  1:617567/1‑41 (MQ=255)                                                             cGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCTTGCATGTAGTGga           <  1:180471/70‑1 (MQ=255)                                                             cGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGga           <  1:3045120/70‑1 (MQ=255)                                                             cGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGga           <  1:115108/70‑1 (MQ=255)                                                              ggATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGgatg         <  1:1051507/71‑1 (MQ=255)                                                              ggATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGgat          >  1:2498510/1‑70 (MQ=255)                                                              ggATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGgat          >  1:1712621/1‑70 (MQ=255)                                                               gATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGgatga        <  1:1510895/71‑1 (MQ=255)                                                                 tA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGatgatg      <  1:2111386/71‑1 (MQ=255)                                                                  a‑aCGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGt     >  1:2848019/1‑71 (MQ=255)                                                                  a‑aCGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGt     >  1:1109247/1‑71 (MQ=255)                                                                    aCGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTc                                        <  1:2784786/35‑1 (MQ=255)                                                                    aCGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGt     <  1:284503/70‑1 (MQ=255)                                                                      gTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTca  <  1:3765323/71‑1 (MQ=255)                                                                      |                                                                       TTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCA  >  minE/608632‑608770

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑