| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 724,481:1 | +C | 10.0% | coding (287/960 nt) | rluC → | 23S rRNA pseudouridylate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 724,481 | 1 | . | C | 10.0% | 204.0 / 25.7 | 60 | coding (287/960 nt) | rluC | 23S rRNA pseudouridylate synthase |
| Reads supporting (aligned to +/- strand): ref base . (31/23); new base C (2/4); total (33/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.94e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01 | |||||||||||
CTGAGCGGGAAGAAGAGGCGGTTTCGCCACATCTGCAAAAGGTGGCGGCGCTGGCGGACGTCATCTT‑ATATGAAGATGATCACATCCTGGTGCTGAATAAACCTTCCGGTACGGCGGTACATGGCGGCAGTGGTT > minE/724415‑724549 | cTGAGCGGGAAGAAGAGGCGGTTTCGCCACATCTGCAAAAGGTGGCGGCGCTGGCGGACGTCATCTt‑ata < 1:2703634/70‑1 (MQ=255)cTGAGCGGGAAGAAGAGGCGGTTTCGCCACATCTGCAAAAGGTGGCGGCGCTGGCGGACGTCATCTT‑atat > 1:1062477/1‑71 (MQ=255) gAGCGGGAAGAAGAGGCGGTTTCGCCACATCTGCAAAAGGTGGCGGCGCTGGCGGACGTCATCTT‑ATATg > 1:3487787/1‑70 (MQ=255) gAGCGGGAAGAAGAGGCGGTTTCGCCACATCTGCAAAAGGTGGCGGCGCTGGCGGACGTCATCTT‑ATATg > 1:1433102/1‑70 (MQ=255) aGCGGGAAGAAGAGGCGGTTTCGCCACATCTGCAAAAGGTGGCGGCGCTGGCGGACGTCATCTT‑ATATg > 1:1121806/1‑69 (MQ=255) gggAAGAAGAGGCGGTTTCGCCACATCTGCAAAAGGTGGCGGCGCTGGCGGACGTCATCTT‑ATATGAAgat < 1:2564688/71‑1 (MQ=255) agaagaGGCGGTTTCGCCACATCTGCAAAAGGTGGCGGCGCTGGCGGACGTCATCTT‑ATATGaa < 1:2075886/64‑1 (MQ=255) agaagaGGCGGTTTCGCCACATCTGCAAAAGGTGGCGGCGCTGGCGGACGTCATCTT‑ATATGaa < 1:95637/64‑1 (MQ=255) agaagaGGCGGTTTCGCCACATCTGCAAAAGGTGGCGGCGCTGGCGGACGTCATCTT‑ATATGAAga > 1:736010/1‑66 (MQ=255) agaagaGGCGGTTTCGCCACATCTGCAAAAGGTGGCGGCGCTGGCGGACGTCATCTT‑ATATGAAGATGATc < 1:652954/71‑1 (MQ=255) agaagaGGCGGTTTCGCCACATCTGCAAAAGGTGGCGGCGCTGGCGGACGTCATCTT‑ATATGAAGATGATc < 1:2876918/71‑1 (MQ=255) agaagaGGCGGTTTCGCCACATCTGCAAAAGGTGGCGGCGCTGGCGGACGTCATCTT‑ATATGAAGATGATc < 1:2351795/71‑1 (MQ=255) agaagaGGCGGTTTCGCCACATCTGCAAAAGGTGGCGGCGCTGGCGGACGGCATCTT‑ATATGAAGATGATc < 1:1153155/71‑1 (MQ=255) gagGCGGTTTCGCCACATCTGCAAAAGGTGGCGGCGCTGGCGGACGTCATCTt‑ata > 1:2688952/1‑56 (MQ=255) gagGCGGTTTCGCCACATCTGCAAAAGGTGGCGGCGCTGGCGGACGTCATCTt‑ata > 1:2609385/1‑56 (MQ=255) gagGCGGTTTCGCCACATCTGCAAAAGGTGGCGGCGCTGGCGGACGTCATCTt‑ata > 1:2281560/1‑56 (MQ=255) agGCGGTTTCGCCACATCTGCAAAAGGTGGCGGCGCTGGCGGACGTCATCTT‑ATATGAAGATGATcac > 1:3728835/1‑68 (MQ=255) agGCGGTTTCGCCACATCTGCAAAAGGTGGCGGCGCTGGCGGACGTCATCTT‑ATATGAAGATGATcac > 1:3675809/1‑68 (MQ=255) ggCGGTTTCGCCACATCTGCAAAAGGTGGCGGCGCTGGCGGACGTCATCTT‑ATATGAAGATGATCACATcc > 1:510292/1‑71 (MQ=255) gCGGTTTCGCCACATCTGCAAAAGGTGGCGGCGCTGGCGGACGTCATCTT‑ATATGAAGATGATCACATc > 1:3578577/1‑69 (MQ=255) gCGGTTTCGCCACATCTGCAAAAGGTGGCGGCGCTGGCGGACGTCATCTT‑ATATGAAGATGATCACATc > 1:2354177/1‑69 (MQ=255) ggTTTCGCCACATCTGCAAAAGGTGGCGGCGCTGGCGGACGTCATCTT‑ATATGAAGATGATCACATCCTgg > 1:2423243/1‑71 (MQ=255) cGCCACATCTGCAAAAGGTGGCGGCGCTGGCGGACGTCATCTT‑ATATGAAGATGATCACATCCTGGTGCt < 1:1946543/70‑1 (MQ=255) gCCACATCTGCAAAAGGTGGCGGCGCTGGCGGACGTCATCTT‑ATATGAAGATGATCACATCCTGGTg < 1:3399475/67‑1 (MQ=255) gCCACATCTGCAAAAGGTGGCGGCGCTGGCGGACGTCATCTT‑ATATGAAGATGATCACATCCTGGTGCTg < 1:192704/70‑1 (MQ=255) ccACATCTGCAAAAGGTGGCGGCGCTGGCGGACGTCATCTT‑ATATGAAGATGATCAAATCCTGGTGCTGa > 1:361702/1‑70 (MQ=255) cacaTCTGCAAAAGGTGGCGGCGCTGGCGGACGTCATCTT‑ATATGAAGATGATCACATCCTGGTGCTGAAt < 1:2924118/71‑1 (MQ=255) caTCTGCAAAAGGTGGCGGCGCTGGCGGACGTCATCTT‑ATATGAAGATGATc < 1:1821595/52‑1 (MQ=255) tCTGCAAAAGGTGGCGGCGCTGGCGGACGTCATCTT‑ATATGAAGATGATCACATCCTgg < 1:302095/59‑1 (MQ=255) tCTGCAAAAGGTGGCGGCGCTGGCGGACGTCATCTT‑ATATGAAGATGATCACATCCTGGTGCTGAATAAAc > 1:252207/1‑71 (MQ=255) cAAAAGGTGGCGGCGCTGGCGGACGTCATCTT‑ATATGAAGATGATCACATCCTGGt > 1:274405/1‑56 (MQ=255) cAAAAGGTGGCGGCGCTGGCGGACGTCATCTT‑ATATGAAGATGATCACATCCTGGTGCTGAATAAAc < 1:223058/67‑1 (MQ=255) cAAAAGGTGGCGGCGCTGGCGGACGTCATCTT‑ATATGAAGATGATCACATCCTGGTGCTGAATAAAc > 1:1761470/1‑67 (MQ=255) cAAAAGGTGGCGGCGCTGGCGGACGTCATCTT‑ATATGAAGATGATCACATCCTGGTGCTGAATAAACCTTc > 1:159988/1‑71 (MQ=255) aaaGGTGGCGGCGCTGGCGGACGTCATCTT‑ATATGAAGATGATCACATCCTGGTGCTGAATAAACCTTcc > 1:2677790/1‑70 (MQ=255) aaaGGTGGCGGCGCTGGCGGACGTCATCTT‑ATATGAAGATGATCACATCCTGGTGCTGAATAAACCTTCCg > 1:2381953/1‑71 (MQ=255) aaaGGTGGCGGCGCTGGCGGACGTCATCTT‑ATATGAAGATGATCACATCCTGGTGCTGAATAAACCTTCCg < 1:3425753/71‑1 (MQ=255) aaaGGTGGCGGCGCTGGCGGACGTCATCTT‑ATATGAAGATGATCACATCCTGGTGCTGAATAAACCTTCCg < 1:3111083/71‑1 (MQ=255) aaaGGTGGCGGCGCTGGCGGACGTCATCTT‑ATATGAAGATGATCACATCCTGGTGCTGAATAAACCTTCCg > 1:704455/1‑71 (MQ=255) aaaGGTGGCGGCGCTGGCGGACGTCATCTT‑ATATGAAGATGATCACATCCTGGTGCTGAATAAACCTTCCg > 1:2039763/1‑71 (MQ=255) aaaGGTGGCGGCGCTGGCGGACGTCATCTT‑ATATGAAGATGATCACATCCTGGTGCTGAATAAACCTTCCg < 1:1620963/71‑1 (MQ=255) aaGGTGGCGGCGCTGGCGGACGTCATCTT‑ATATGAAGATGATCACATCCTGGTGCTGAATAAACCTTCCgg > 1:544815/1‑71 (MQ=255) aGGTGGCGGCGCTGGCGGACGTCATCTT‑ATATGAAGATGATca > 1:1730416/1‑43 (MQ=255) tGGCGGCGCTGGCGGACGTCATCTT‑ATATGAAGATGATCACATCCTGGTGCTGAATAAACCTTCCGGTAcg > 1:1448961/1‑71 (MQ=255) tGGCGGCGCTGGCGGACGTCATCTT‑ATATGAAGATGATCACATCCTGGTGCTGAATAAACCTTCCGGTAcg > 1:1412349/1‑71 (MQ=255) ggcggcGCTGGCGGACGTCATCTT‑ATATGAAGATGATCACATCCTGGTGCTGAATa < 1:2571812/56‑1 (MQ=255) gcggcgCTGGCGGACGTCATCTT‑ATATGAAGATGATCACATCCTGGTGCTGAATAAACCTTCCGGTAcggc > 1:2116782/1‑71 (MQ=255) tGGCGGACGTCATCTT‑ATATGAAGATGATCACATCCTGGTGCTGAATAAACCTTCCGGTACGGCGGTACAt > 1:476555/1‑71 (MQ=255) gCGGACGTCATCTT‑ATATGAAGATGATCACATCCTGGTGCTGAATAAACCTTCCGGTACGGCGGTACAt < 1:2002386/69‑1 (MQ=255) gCGGACGTCATCTT‑ATATGAAGATGATCACATCCTGGTGCTGAATAAACCTTCCGGTACGGCGGTACATgg < 1:1449965/71‑1 (MQ=255) gatCATCTTCATAT‑AAGATGACCACATCCTGGTGCTGAATAAACCTTCCGGTACGGCGGTACATggcg < 1:506781/66‑1 (MQ=37) gatCATCTTCATAT‑AAGATGACCACATCCTGGTGCTGAATAAACCTTCCGGTACGGCGGTACATggcg < 1:1100379/66‑1 (MQ=37) gatCATCTTCATAT‑AAGATGACCACATCCTGGTGCTGAATAAACCTTCCGGTACGGCGGTACATggcg < 1:1712763/66‑1 (MQ=37) cGTCATCTT‑ATATGAAGATGATCACATCCTGGTGCTGAATAAACCTTCCGGTACGGCGGTACATggcgg > 1:1483304/1‑69 (MQ=255) gTCATCTT‑ATATGAAGATGATCACATCCTGGTGCTGAATAAACCTTCCGGTACGGCGGTACATGGCGGCAg < 1:3715022/71‑1 (MQ=255) gTCATCTT‑ATATGAAGATGATCACATCCTGGTGCTGAATAAACCTTCCGGTACGGCGGTACATGGCGGCAg > 1:3051149/1‑71 (MQ=255) atCATCTTCATAT‑AAGATGATCACATCCTGGTGCTGAATAAACCTTCCGGTACGGCGGTACATggcgg > 1:864379/2‑68 (MQ=38) atCATCTTCATAT‑AAGATGATCACATCCTGGTGCTGAATAAACCTTCCGGTACGGCGGTACATggcgg > 1:2017459/2‑68 (MQ=38) atCATCTTCATAT‑AAGATGACCACATCCTGGTGCTGAATAAACCTTCCGGTACGGCGGTACATggcg < 1:634243/66‑1 (MQ=37) tCATCTT‑ATATGAAGATGATCACATCCTGGTGCTGAATAAACCTTCCGGTACGGCGGTACATgg < 1:1492587/64‑1 (MQ=255) tCATCTT‑ATATGAAGATGATCACATCCTGGTGCTGAATAAACCTTCCGGTACGGCGGTACATgg < 1:2645892/64‑1 (MQ=255) cATCTT‑ATATGAAGATGATCACATCCTGGTGCTGAATAAACCTTCCg > 1:2077664/1‑47 (MQ=255) cATCTT‑ATATGAAGATGATCACATCCTGGTGCTGAATAAACCTTCCGGTACGGCGGTACa > 1:404687/1‑60 (MQ=255) tCTT‑ATATGAAGATGATCACATCCTGGTGCTGAATAAACCTTCCGGTACGGCGGTACATggcgg > 1:1870086/1‑64 (MQ=255) tCTT‑ATATGAAGATGATCACATCCTGGTGCTGAATAAACCTTCCGGTACGGCGGTACATGGCGGCAGTGGt > 1:1575678/1‑71 (MQ=255) tt‑ATATGAAGATGATCACATCCTGGTGCTGAATAAACCTTCCGGTACGGCGGTACATGGCGGCAGTGGtt > 1:1208846/1‑70 (MQ=255) tt‑ATAAGAAGATGATCACATCCTGGTGCTGAATAAACCTTCCGGTACGGCGGTAc > 1:1608227/1‑55 (MQ=255) | CTGAGCGGGAAGAAGAGGCGGTTTCGCCACATCTGCAAAAGGTGGCGGCGCTGGCGGACGTCATCTT‑ATATGAAGATGATCACATCCTGGTGCTGAATAAACCTTCCGGTACGGCGGTACATGGCGGCAGTGGTT > minE/724415‑724549 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |