| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 863,791 | A→C | 12.3% | Y31S (TAT→TCT) ‡ | yciQ → | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 863,791 | 0 | A | C | 12.3% | 129.1 / 16.6 | 57 | Y31S (TAT→TCT) ‡ | yciQ | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (24/26); new base C (4/3); total (28/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.06e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.93e-01 | |||||||||||
TTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAG > minE/863722‑863859 | ttttGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑At > 1:623836/1‑71 (MQ=255)ttttGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑At > 1:3597436/1‑71 (MQ=255)ttttGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑At > 1:1377647/1‑71 (MQ=255) ttGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATg < 1:906306/70‑1 (MQ=255) ttGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATg < 1:3208089/70‑1 (MQ=255) cTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATgaaga < 1:1704669/71‑1 (MQ=255) cTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATgaag < 1:2672846/70‑1 (MQ=255) cTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATgaag < 1:206318/70‑1 (MQ=255) cTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATgaag < 1:1658272/70‑1 (MQ=255) gTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑a > 1:1817839/1‑63 (MQ=255) ttGATAGCAGGGCTG‑TTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAgg < 1:274555/70‑1 (MQ=255) gATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAggg < 1:1510241/70‑1 (MQ=255) gATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGa > 1:1151319/1‑71 (MQ=255) aGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGc > 1:981965/1‑71 (MQ=255) aGGGCTGTTTGTATCATCTCTAAGTTACGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGc > 1:68991/1‑71 (MQ=255) gggCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGc > 1:2622985/1‑70 (MQ=255) tttGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑CATaagaag < 1:94091/55‑1 (MQ=37) tttGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑CATaagaag < 1:1533414/55‑1 (MQ=37) tttGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑CATaagaag < 1:63038/55‑1 (MQ=37) tttGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCttt < 1:1239832/69‑1 (MQ=255) tGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑CATAAGAAggg > 1:2685518/1‑55 (MQ=25) tGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGaagaag > 1:1158005/1‑53 (MQ=255) tGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATg > 1:3725011/1‑71 (MQ=255) tGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATg > 1:3652887/1‑71 (MQ=255) tcatcTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑CATAAGAAgg > 1:1453542/1‑50 (MQ=25) tcatcTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTg < 1:1254451/70‑1 (MQ=255) tcatcTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTg < 1:3172613/70‑1 (MQ=255) tcatcTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTg < 1:3011424/70‑1 (MQ=255) tcatcTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGa < 1:1534084/71‑1 (MQ=255) atcTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGa < 1:1063348/50‑1 (MQ=255) atcTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGc > 1:2076942/1‑56 (MQ=255) tctAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCa > 1:1435742/1‑71 (MQ=255) aaGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCActc > 1:1687068/1‑71 (MQ=255) aaGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCActc > 1:1966979/1‑71 (MQ=255) aGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATgaa > 1:2963731/1‑36 (MQ=255) aGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCAct < 1:2537342/69‑1 (MQ=255) gTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTg < 1:955882/71‑1 (MQ=255) gTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTg < 1:1871918/71‑1 (MQ=255) ttATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGc < 1:24186/71‑1 (MQ=255) aTGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGa > 1:2490978/1‑58 (MQ=255) aTGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGa > 1:750182/1‑58 (MQ=255) aaaCACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGAt < 1:2855137/70‑1 (MQ=255) aaaCACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATg < 1:2327904/71‑1 (MQ=255) aaaCACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATg > 1:1223584/1‑71 (MQ=255) cacGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATgggg < 1:2454551/71‑1 (MQ=255) cGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTg > 1:2795908/1‑56 (MQ=255) cGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTg > 1:839341/1‑71 (MQ=255) gagaTCCCTTCTT‑ATGAATAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTg < 1:3678386/54‑1 (MQ=255) aTCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGa > 1:1588448/1‑71 (MQ=255) cccTTCTTCATG‑AGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACa > 1:3352957/1‑71 (MQ=38) cccTTCTT‑CATAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCaa > 1:2137300/1‑52 (MQ=25) cccTTCTT‑CATAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCaa > 1:865887/1‑52 (MQ=25) cccTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATg < 1:3712868/35‑1 (MQ=255) ttctt‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGaa < 1:2655560/37‑1 (MQ=255) ttctt‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGaa < 1:3412391/37‑1 (MQ=255) ttctt‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGaa < 1:3028768/37‑1 (MQ=255) ttctt‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATc > 1:164709/1‑70 (MQ=255) t‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAg > 1:2284058/1‑70 (MQ=255) aTGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAAc < 1:231805/45‑1 (MQ=255) | TTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAG > minE/863722‑863859 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |