| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 1,270,387 | +G | coding (638/816 nt) | yedP → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,270,384 | 1 | . | G | 76.3% | 323.6 / 93.2 | 114 | coding (635/816 nt) | yedP | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (0/27); new base G (0/87); total (0/114) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 120 total aligned reads displayed.
GCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑T‑GGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGA > minE/1270323‑1270426 | gCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑t > 1:115736/1‑62 (MQ=255)gCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑t > 1:121417/1‑62 (MQ=255)gCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑t > 1:221121/1‑62 (MQ=255)gCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑t > 1:586972/1‑62 (MQ=255) ctttCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:168713/59‑1 (MQ=255) cTATCAACAATTGTCGGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:1123339/62‑1 (MQ=255) cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATTGGCCAAACGAt < 1:1000987/62‑1 (MQ=255) cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:213373/62‑1 (MQ=255) cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:221809/62‑1 (MQ=255) cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:544628/62‑1 (MQ=255) cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:263134/62‑1 (MQ=255) cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:275655/62‑1 (MQ=255) cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:276004/62‑1 (MQ=255) cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:281159/62‑1 (MQ=255) cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:708829/62‑1 (MQ=255) cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:315786/62‑1 (MQ=255) cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:194640/62‑1 (MQ=255) cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:326240/62‑1 (MQ=255) cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:36094/62‑1 (MQ=255) cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:366731/62‑1 (MQ=255) cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:386029/62‑1 (MQ=255) cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:664954/62‑1 (MQ=255) cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:427744/62‑1 (MQ=255) cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:437950/62‑1 (MQ=255) cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:446596/62‑1 (MQ=255) cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:647481/62‑1 (MQ=255) cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:607142/62‑1 (MQ=255) cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:566712/62‑1 (MQ=255) cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:49500/62‑1 (MQ=255) cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:501681/62‑1 (MQ=255) cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:1021059/62‑1 (MQ=255) cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:879721/62‑1 (MQ=255) cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:1087068/62‑1 (MQ=255) cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:109084/62‑1 (MQ=255) cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:854898/62‑1 (MQ=255) cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:109628/62‑1 (MQ=255) cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:810419/62‑1 (MQ=255) cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:1053403/62‑1 (MQ=255) cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:1112745/62‑1 (MQ=255) cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:112073/62‑1 (MQ=255) cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:1131718/62‑1 (MQ=255) cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:1135178/62‑1 (MQ=255) cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:794403/62‑1 (MQ=255) cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:787859/62‑1 (MQ=255) cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:1192860/62‑1 (MQ=255) cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:1020673/62‑1 (MQ=255) cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:769925/62‑1 (MQ=255) cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:905840/62‑1 (MQ=255) cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:138572/62‑1 (MQ=255) cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:170213/62‑1 (MQ=255) cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:764659/62‑1 (MQ=255) tATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:983482/61‑1 (MQ=255) tATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:547108/61‑1 (MQ=255) tATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:879791/61‑1 (MQ=255) tATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:684798/61‑1 (MQ=255) tATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:80246/61‑1 (MQ=255) tATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:697792/61‑1 (MQ=255) tATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:712559/61‑1 (MQ=255) tATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:715955/61‑1 (MQ=255) tATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:744355/61‑1 (MQ=255) tATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:766002/61‑1 (MQ=255) tATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:1008893/61‑1 (MQ=255) tATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:490829/61‑1 (MQ=255) tATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:1190574/61‑1 (MQ=255) tATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:1135616/61‑1 (MQ=255) tATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:179532/61‑1 (MQ=255) tATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:1104415/61‑1 (MQ=255) tATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:1056971/61‑1 (MQ=255) tATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:309582/61‑1 (MQ=255) tATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:1065463/61‑1 (MQ=255) tATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:1095788/61‑1 (MQ=255) tATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:40386/61‑1 (MQ=255) tATCAACAATTGTCAGACAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:837092/61‑1 (MQ=255) tATCAACAATTGTCACGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:121188/61‑1 (MQ=255) aTCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:273976/60‑1 (MQ=255) tCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:608257/59‑1 (MQ=255) gTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt < 1:951989/50‑1 (MQ=255) ccAAACGCCCAACCACACTTGGCCGG‑GGGCGATGGGCCAAACGAt < 1:298294/44‑1 (MQ=255) aaCGCCCAACCACACTTGGCCGG‑GGGCGATGGGCCAAACGAt < 1:496333/42‑1 (MQ=255) cGCCCAACCATACTTGGCC‑T‑GGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGa < 1:636999/62‑1 (MQ=255) cGCCCAACCACACTTGGCC‑T‑GGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGa < 1:241620/62‑1 (MQ=255) cGCCCAACCACACTTGGCC‑T‑GGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGa < 1:819919/62‑1 (MQ=255) cGCCCAACCACACTTGGCC‑T‑GGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGa < 1:1107999/62‑1 (MQ=255) cGCCCAACCACACTTGGCC‑T‑GGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGa < 1:1012164/62‑1 (MQ=255) cGCCCAACCACACTTGGCC‑T‑GGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGa < 1:906473/62‑1 (MQ=255) cGCCCAACCACACTTGGCC‑T‑GGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGa < 1:170475/62‑1 (MQ=255) cGCCCAACCACACTTGGCC‑T‑GGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGa < 1:765493/62‑1 (MQ=255) cGCCCAACCACACTTGGCC‑T‑GGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGa < 1:268539/62‑1 (MQ=255) cGCCCAACCACACTTGGCC‑T‑GGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGa < 1:70652/62‑1 (MQ=255) cGCCCAACCACACTTGGCC‑T‑GGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGa < 1:401922/62‑1 (MQ=255) cGCCCAACCACACTTGGCC‑T‑GGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGa < 1:681190/62‑1 (MQ=255) cGCCCAACCACACTTGGCC‑T‑GGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGa < 1:661590/62‑1 (MQ=255) cGCCCAACCACACTTGGCC‑T‑GGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGa < 1:466292/62‑1 (MQ=255) cGCCCAACCACACTTGGCC‑T‑GGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGa < 1:631065/62‑1 (MQ=255) cGCCCAACCACACTTGGCC‑T‑GGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGa < 1:477627/62‑1 (MQ=255) cGCCCAACCACACTTGGCC‑T‑GGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGa < 1:580042/62‑1 (MQ=255) cGCCCAACCACACTTGGCC‑T‑GGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGa < 1:556569/62‑1 (MQ=255) cGCCCAACCACACTTGGCC‑G‑GGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGa < 1:808970/62‑1 (MQ=255) gCCCAACCACACTTGGCC‑T‑GGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGa < 1:489765/61‑1 (MQ=255) ttGGCC‑T‑GGGCGATGGGCCAAACGGTGCGCCCTTACTGGAGGTAATGGa < 1:944690/49‑1 (MQ=255) | GCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑T‑GGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGA > minE/1270323‑1270426 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |