Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,920,214	+C	coding (686/1194 nt)	ygeD ←	predicted inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,920,210	1	.	C	100.0%	123.0 / NA	34	G230G (GGA→GGG)	ygeD	predicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (0/34);  total (0/34)

CCGCT‑CCCCAGAATAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAG  >  minE/1920206‑1920266      |                                                         ccGCTCCCCCAGAATAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAg  <  1:842067/62‑1 (MQ=255) ccGCTCCCCCAGAATAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAg  <  1:589340/62‑1 (MQ=255) ccGCTCCCCCAGAATAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAg  <  1:596137/62‑1 (MQ=255) ccGCTCCCCCAGAATAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAg  <  1:61503/62‑1 (MQ=255) ccGCTCCCCCAGAATAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAg  <  1:654519/62‑1 (MQ=255) ccGCTCCCCCAGAATAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAg  <  1:695448/62‑1 (MQ=255) ccGCTCCCCCAGAATAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAg  <  1:70715/62‑1 (MQ=255) ccGCTCCCCCAGAATAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAg  <  1:723627/62‑1 (MQ=255) ccGCTCCCCCAGAATAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAg  <  1:840437/62‑1 (MQ=255) ccGCTCCCCCAGAATAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAg  <  1:57159/62‑1 (MQ=255) ccGCTCCCCCAGAATAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAg  <  1:85973/62‑1 (MQ=255) ccGCTCCCCCAGAATAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAg  <  1:885710/62‑1 (MQ=255) ccGCTCCCCCAGAATAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAg  <  1:887306/62‑1 (MQ=255) ccGCTCCCCCAGAATAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAg  <  1:931336/62‑1 (MQ=255) ccGCTCCCCCAGAATAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAg  <  1:942319/62‑1 (MQ=255) ccGCTCCCCCAGAATAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAg  <  1:946478/62‑1 (MQ=255) ccGCTCCCCCAGAATAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAg  <  1:966533/62‑1 (MQ=255) ccGCTCCCCCAGAATAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAg  <  1:288813/62‑1 (MQ=255) ccGCTCCCCCAGAATAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAg  <  1:1053291/62‑1 (MQ=255) ccGCTCCCCCAGAATAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAg  <  1:1160107/62‑1 (MQ=255) ccGCTCCCCCAGAATAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAg  <  1:1186325/62‑1 (MQ=255) ccGCTCCCCCAGAATAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAg  <  1:1192605/62‑1 (MQ=255) ccGCTCCCCCAGAATAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAg  <  1:142312/62‑1 (MQ=255) ccGCTCCCCCAGAATAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAg  <  1:197950/62‑1 (MQ=255) ccGCTCCCCCAGAATAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAg  <  1:240740/62‑1 (MQ=255) ccGCTCCCCCAGAATAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAg  <  1:284143/62‑1 (MQ=255) ccGCTCCCCCAGAATAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAg  <  1:1000917/62‑1 (MQ=255) ccGCTCCCCCAGAATAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAg  <  1:31377/62‑1 (MQ=255) ccGCTCCCCCAGAATAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAg  <  1:397998/62‑1 (MQ=255) ccGCTCCCCCAGAATAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAg  <  1:398191/62‑1 (MQ=255) ccGCTCCCCCAGAATAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAg  <  1:444258/62‑1 (MQ=255) ccGCTCCCCCAGAATAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAg  <  1:496727/62‑1 (MQ=255) ccGCTCCCCCAGAATAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAg  <  1:519864/62‑1 (MQ=255) ccGCTCCCCCAGAATAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAg  <  1:543115/62‑1 (MQ=255)      |                                                         CCGCT‑CCCCAGAATAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAG  >  minE/1920206‑1920266

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑