| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,276,691 | Δ1 bp | 100% | coding (994/1815 nt) | yejA → | predicted oligopeptide transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,276,691 | 0 | T | . | 83.3% | 121.5 / 25.6 | 42 | coding (994/1815 nt) | yejA | predicted oligopeptide transporter subunit |
| Reads supporting (aligned to +/- strand): ref base T (7/0); new base . (20/15); total (27/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.82e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch. | |||||||||||
GGCTGGCGTTTAATATCCAACGTCCGGTATTCAGCGATCGCCGGGTCCGGGAAGCTATCACTCTCGCCTTTGACTTTGAATGGATGAACAAGGCGTTGTTTTACAATGCCTGGAGTCGCACGAACAGTTA > W3110S.gb/2276623‑2276752 | ggctggcGTTTAATATCCAACGTCCGGTATTCAGCGATCGCCGGGTCCGGGAAGCTATCACTCTCGCct > 1:3031700/1‑69 (MQ=255) ggcGTTTAATATCCAACGTCCGGTATTCAGCGATCGCCGGGTCCGGGAAGCTATCACTCTCGCcttgac > 1:2407974/1‑66 (MQ=255) tttAATATCCAACGTCCGGTATTCAGCGATCGCCGGGTCCGGGAAGCTATCACTCTCGcc‑ttgactttg < 1:3357699/69‑1 (MQ=255) tttAATATCCAACGTCCGGTATTCAGCGATCGCCGGGTCCGGGAAGCTATCACTCTCGCctttgactt > 1:679978/1‑68 (MQ=255) ttAATATCCAACGTCCGGTATTCAGCGATCGCCGGGTCCGGGAAGCTATCACTCTCGCctttgactttg < 1:2555683/69‑1 (MQ=255) ttAATATCCAACGTCCGGTATTCAGCGATCGCCGGGTCCGGGAAGCTATCACTCTCGCctttgactttg < 1:698727/69‑1 (MQ=255) tAATATCCAACGTCCGGTATTCAGCGATCGCCGGGTCCGGGAAGCTATCACTCTCGCctttgactttga > 1:3110170/1‑69 (MQ=255) cAACGTCCGGTATTCAGCGCTCGCCGGGTCCGGGAAGCTATCACTCTCGCC‑TTGACTTTGAATGGATg > 1:3026049/1‑68 (MQ=255) cAACGTCCGGTATTCAGCGATCGCCGGGTCCGGGAAGCTATCACTCTCGcc‑ttgactttg < 1:345840/60‑1 (MQ=255) cAACGTCCGGTATTCAGCGATCGCCGGGTCCGGGAAGCTATCACTCTCGcc‑ttgactttg < 1:2171321/60‑1 (MQ=255) cAACGTCCGGTATTCAGCGATCGCCGGGTCCGGGAAGCTATCACTCTCGcc‑ttgactttg < 1:507824/60‑1 (MQ=255) cAACGTCCGGTATTCAGCGATCGCCGGGTCCGGGAAGCTATCACTCTCGCC‑TTGACTTTGAATGGATGa < 1:1169708/69‑1 (MQ=255) gTCGGGTATTCAGCGATCGCCGGGTCCGGGAAGCTATCACTCTCGCC‑TTGACTTTGAATg < 1:2049372/60‑1 (MQ=255) cGGTATTCAGCGATCGCCGGGTCCGGGAAGCTATCACTCTCGCCTTTGACTTTGAATGGATGAACAAgg > 1:681056/1‑69 (MQ=255) ttCAGCGATCGCCGGGTCCGGGAAGCTATCACTCTCGCCTTTGACTTTGAATGGATGAACAAGGCgttg > 1:3275206/1‑69 (MQ=255) ttCAGCGATCGCCGGGTCCGGGAAGCTATCACTCTCGCCTTTGACTTTGAATGGATGAACAAGGCgttg > 1:3686479/1‑69 (MQ=255) tCAGCGATCGCCGGGTCCGTGAAGCTATCACTCTCGCC‑TTGACTTTGAATGGATGAACAAGGCgttgt < 1:310238/68‑1 (MQ=255) tCAGCGATCGCCGGGTCCGGGAAGCTATCACTCTCGCC‑TTGACTTTGAATGGATGAACAAGGCgttgt < 1:1865787/68‑1 (MQ=255) tCAGCGATCGCCGGGTCCGGGAAGCTATCACTCTCGCC‑TTGACTTTGAATGGATGAACAAGGCgttgt < 1:1484622/68‑1 (MQ=255) cAGCGATCGCCGGGTCCGGGAAGCTATCACTCTCGCctttgact > 1:3630403/1‑44 (MQ=255) cAGCGATCGCCGGGTCCGGGAAGCTATCACTCTCGCctttgact > 1:2797118/1‑44 (MQ=255) cAGCGATCGCCGGGTCCGGGAAGCTATCACTCTCGCCTTTGACTTTGAATGGATGAACa > 1:2519247/1‑59 (MQ=255) cAGCGATCGCCGGGTCCGGGAAGCTATCACTCTCGCCTTTGACTTTGAATGGATGAACAAGGCgttgt > 1:2241904/1‑68 (MQ=255) cAGCGATCGCCGGGTCCGGGAAGCTATCACTCTCGCC‑TTGACTTTGAATGTATGAACAAGGCGTTGttt < 1:488967/69‑1 (MQ=255) cAGCGATCGCCGGGTCCGGGAAGCTATCACTCTCGCC‑TTGACTTTGAATGGATGAACAAGGCgttgtt > 1:3536052/1‑68 (MQ=255) cAGCGATCGCCGGGTCCGGGAAGCTATCACTCTCGCC‑TTGACTTTGAATGGATGAACAAGGCGTTGttt > 1:3018183/1‑69 (MQ=255) cGATCGCCGGGTCCGTGAAGCTATCACTCTCGCC‑TTGACTTTGAATGGATGAACAAGGCGTTGTTTTAc < 1:360635/69‑1 (MQ=255) cGATCGCCGGGTCCGGGAAGCTATCACTCTCGCC‑TTGACTTTGAATGTATGAACAAGGCGTTGTTTTa < 1:2017163/68‑1 (MQ=255) cGATCGCCGGGTCCGGGAAGCTATCACTCTCGCC‑TTGACTTTGAATGGGTGAACAAGGCGTTGTTTTAc < 1:718055/69‑1 (MQ=255) cGATCGCCGGGTCCGGGAAGCTATCACTCTCGCC‑TTGACTTTGAATGGATGAACAAGGCGTTGTTTTa < 1:244780/68‑1 (MQ=255) cGATCGCCGGGTCCGGGAAGCTATCACTCTCGCC‑TTGACTTTGAATGGATGAACAAGGCGTTGTTTTAc < 1:2233826/69‑1 (MQ=255) cGATCGCCGGGTCCGGGAAGCTATCACTCTCGCC‑TTGACTTTGAATGGATGAACAAGGCGTTGTTTTAc < 1:1051932/69‑1 (MQ=255) cGCCGGGTCCGGGAAGCTATCACTCTCGcc‑ttgactttg > 1:2092730/1‑39 (MQ=38) cGCCGGGTCCGGGAAGCTATCACTCTCGcc‑ttgactttg < 1:148395/39‑1 (MQ=38) cGCCGGGTCCGGGAAGCTATCACTCTCGcc‑ttgactttg < 1:876148/39‑1 (MQ=38) cGCCGGGTCCGGGAAGCTATCACTCTCGCC‑TTGACTTTGAATGGATGAACAAGGCGTTGTTTTACAAt > 1:1880477/1‑68 (MQ=255) cGCCGGGTCCGGGAAGCTATCACTCTCGCC‑TTGACTTTGAATGGATGAACAAGGCGTTGTTTTACAAt > 1:1567966/1‑68 (MQ=255) cGCCGGGTCCGGGAAGCTATCACTCTCGCC‑TTGACTTTGAATGGATGAACAAGGCGTTGTTTTACAATg > 1:1068319/1‑69 (MQ=255) cGCCGGGTCCGGGAAGCTATCACTCTCGCC‑TTGACTTTGAATGGATGAACAAGGCGTTGTTTTACAATg > 1:742372/1‑69 (MQ=255) cGCCGGGTCCGGGAAGCTATCACTCTCGCC‑TTGACTTTGAATGGATGAACAAGGCGTTGTTTTACAATg > 1:838375/1‑69 (MQ=255) cGCCGGGTCCGGGAAGCTATCACTCTCGCC‑TTGACTTTGAATGGATGAACAAGGCGTTGTTTTACAATg > 1:3337334/1‑69 (MQ=255) gggTCCGGGAAGCTATCACTCTCGCC‑TTGACTTTGAATGGATGAACAAGGCGTTGTTTTACAATGcc > 1:3606685/1‑67 (MQ=255) ccGGGAAGCTATCACTCTCGCC‑TTGACTTTGAATGGATGAACAAGGCGTTGTTTTACAATGCCTGGAGt > 1:712166/1‑69 (MQ=255) gggAAGCTATCACTCTCGCC‑TTGACTTTGAATGGATGAACAAGGCGTTGTTTTACAATGCCTGGAGTCg > 1:1154456/1‑69 (MQ=255) gggAAGCTATCACTCTCGCC‑TTGACTTTGAATGGATGAACAAGGCGTTGTTTTACAATGCCTGGAGTCg > 1:2526450/1‑69 (MQ=255) gggAAGCTATCACTCTCGCC‑TTGACTTTGAATGGATGAACAAGGCGTTGTTTTACAATGCCTGGAGTCg > 1:830565/1‑69 (MQ=255) cTATCACTCTCGCC‑TTGACTTTGAATGGATGAACAAGGCGTTGTTTTACAATGCCTGGAGTCGCACGaa < 1:3007162/69‑1 (MQ=255) cTATCACTCTCGCC‑TTGACTTTGAATGGATGAACAAGGCGTTGTTTTACAATGCCTGGAGTCGCACGaa < 1:1317528/69‑1 (MQ=255) aCTCTCGCCTTTGACTTTGAATGGATGAACAAGGCGTTGTTTTACAATGCCTg > 1:2167467/1‑53 (MQ=255) aCTCTCGCC‑TTGACTTTGAATGGATGAACAAGGCGTTGttt > 1:3659011/1‑41 (MQ=38) aCTCTCGCC‑TTGACTTTGAATGGATGAACAAGGCGTTGttt > 1:2128058/1‑41 (MQ=38) aCTCTCGCC‑TTGACTTTGAATGGATGAACAAGGCGTTGttt > 1:1632618/1‑41 (MQ=38) aCTCTCGCC‑TTGACTTTGAATGGATGAACAAGGCGTTGTTTTACAATGCCTGGAGTCGCACGAACAGtt > 1:977366/1‑69 (MQ=255) aCTCTCGCC‑TTGACTTTGAATGGATGAACAAGGCGTTGTTTTACAATGCCTGGAGTCGCACGAACAGtt > 1:1898150/1‑69 (MQ=255) aCTCTCGCC‑TTGACTTTGAATGGATGAACAAGGCGTTGTTTTACAATGCCTGGAGTCGCACGAACAGtt > 1:3207543/1‑69 (MQ=255) ctctCGCCTTTGACTTTGAATGGATGAACAAGGCGTTGTTTTACAATGCCTGGAGTCGCACGAACAGtt > 1:1387241/1‑69 (MQ=255) tctcGCC‑TTGACTTTGAATGGATGAACAAGGCGTTGTTTTACAATGCCTGGAGTCGCACGAACAGTTa < 1:1140433/68‑1 (MQ=255) tttgactttgaATGGATGAACAAGGCGTTGTTTTAc < 1:390546/36‑1 (MQ=255) | GGCTGGCGTTTAATATCCAACGTCCGGTATTCAGCGATCGCCGGGTCCGGGAAGCTATCACTCTCGCCTTTGACTTTGAATGGATGAACAAGGCGTTGTTTTACAATGCCTGGAGTCGCACGAACAGTTA > W3110S.gb/2276623‑2276752 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |