Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 2,866,848:1 +T 40.7% coding (562/1140 nt) nlpD ← predicted outer membrane lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,866,8481.T40.7% 24.0 / 101.9 54coding (562/1140 nt)nlpDpredicted outer membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base . (20/12);  new base T (14/8);  total (34/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

CAGAATACGTAATTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCC‑TTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCGGAAGCATTACC  >  W3110S.gb/2866779‑2866914
                                                                      |                                                                  
cAGAATACGTAATTGTCGGTTGCAACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCC‑t                                                                   >  1:1148487/1‑71 (MQ=255)
 aGAATACGTAATTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCC‑tt                                                                  >  1:597195/1‑71 (MQ=255)
       cGTAATTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCCTTTGctct                                                             >  1:2623054/1‑71 (MQ=255)
       cGTAATTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCCTTTGctct                                                             >  1:263602/1‑71 (MQ=255)
       cGTAATTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCCTTTGctct                                                             >  1:1580530/1‑71 (MQ=255)
       cGTAATTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCCTTTGctct                                                             >  1:1521023/1‑71 (MQ=255)
       cGTAATTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCC‑TTGctct                                                             <  1:399425/70‑1 (MQ=255)
       cGTAATTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCC‑TTGCTctg                                                            <  1:856038/71‑1 (MQ=255)
       cGTAATTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCC‑TTGCTctg                                                            <  1:478401/71‑1 (MQ=255)
         tAATTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCC‑TTGct                                                               >  1:1310697/1‑66 (MQ=255)
           aTTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCCTTTGCTctgctg                                                         <  1:2603326/71‑1 (MQ=255)
           aTTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCCTTTGCTctgctg                                                         >  1:880719/1‑71 (MQ=255)
           aTTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCCTTTGCTctgctg                                                         >  1:914956/1‑71 (MQ=255)
           aTTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCCTTTGCTctgctg                                                         >  1:2265367/1‑71 (MQ=255)
           aTTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCCTTTGCTctgct                                                          <  1:2225957/70‑1 (MQ=255)
           aTTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCC‑TTGCTCtgctgc                                                        >  1:57879/1‑71 (MQ=255)
            ttGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCC‑TTGCTctgc                                                           >  1:1654308/1‑67 (MQ=255)
            ttGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACGCC‑TTGCTctgc                                                           >  1:2553697/1‑67 (MQ=255)
             tGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCC‑TTGCTCTGCTGCGt                                                      >  1:1729639/1‑71 (MQ=255)
              gTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCCTTTGCTCTGCTGCGt                                                      >  1:1384591/1‑71 (MQ=255)
              gTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCC‑t                                                                   >  1:1732512/1‑57 (MQ=255)
              gTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCC‑TTGCTCTGCTGCGt                                                      >  1:933373/1‑70 (MQ=255)
                 ggTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCCTTTGCTCTGCTGCGTCgg                                                   <  1:700690/71‑1 (MQ=255)
                     gCGACGCAACAGCAACGGTGTAATTTTGTGCAGGCTTGATCACAACTCCTTTGCTCTGCTGCGTCGGCCTg                                               >  1:2052981/1‑71 (MQ=255)
                     gCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCCTTTGCTCTGCTGCGt                                                      >  1:864685/1‑64 (MQ=255)
                     gCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCC‑tttg                                                                >  1:221788/1‑51 (MQ=255)
                             aCAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCCTTTGCTCTGCTGCGTCGGCCTggg                                             <  1:917987/65‑1 (MQ=255)
                             aCAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCC‑TTGCTCTGCTGCGTCGGCCTGGGTAATGGc                                      <  1:1128059/71‑1 (MQ=255)
                             aCAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCC‑TTGCTCTGCTGCGTCGGCCTGGGTAATGGc                                      <  1:1147352/71‑1 (MQ=255)
                                gCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCC‑TTGCTCTGCTGCGTCGGCCTGGGTAATGGCAtt                                   >  1:2564855/1‑71 (MQ=255)
                                gCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCC‑TTGCTCTGCTGCGTCGGCCTGGGTAATGGCAt                                    >  1:2660870/1‑70 (MQ=255)
                                  aaCGGTGGAATTTTGTGCAGGCTTGATCACAACTCC‑t                                                                   >  1:1470557/1‑37 (MQ=255)
                                   aCGGTGGAATTTTGTGCAGGCTTGATCACAACTCC‑TTGCTCTGCTGCGTCGGCCTGGGt                                            <  1:795552/59‑1 (MQ=255)
                                     ggTGGAATTTTGTGCAGGCTTGATCACAACTCC‑TTGCTCTGCTGCGTCGGCCTGGGTaa                                          >  1:160570/1‑59 (MQ=255)
                                       tGGAATTTTGTGCAGGCTTGATCACAACTCCTTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGc                              <  1:2723406/70‑1 (MQ=255)
                                         gAATTTTGTGCAGGCTTGATCACAACTCC‑TTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGt                          <  1:1904300/71‑1 (MQ=255)
                                         gAATTTTGTGCAGGCTTGATCACAACTCC‑TTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGt                          <  1:2537410/71‑1 (MQ=255)
                                         gAATTTTGTGCAGGCTTGATCACAACTCC‑TTGCTCTGCTGCGTCGGCCT‑GGTAATGGCATTTCCGCCAGTg                         <  1:2381135/71‑1 (MQ=255)
                                           aTTTTGTGCAGGCTTGATCACAACTCC‑TTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGa                        >  1:337196/1‑71 (MQ=255)
                                            ttttGTGCAGGCTTGATCACAACTCCTTTGCTCTGCTGCGTCGGCCTGGGTaa                                          >  1:1642350/1‑53 (MQ=255)
                                            ttttGTGCAGGCTTGATCACAACTCCTTTCCTCTGCTGCGTCGGCCTGGGt                                            >  1:175392/1‑51 (MQ=255)
                                              ttGTGCAGGCTTGATCACAACTCC‑TTGCTCTGCTGCGTCGGCCTGGGTaa                                          >  1:944543/1‑50 (MQ=255)
                                              ttGTGCAGGCTTGATCACAACTCC‑TTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTc                                 >  1:1113182/1‑59 (MQ=255)
                                              ttGTGCAGGCTTGATCACAACTCC‑TTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTc                                 >  1:1346376/1‑59 (MQ=255)
                                                 tgCAGGCTTGATCACAACTCC‑TTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCg                  <  1:781871/71‑1 (MQ=255)
                                                       cTTGATCACAACTCCTTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCg             >  1:1913166/1‑71 (MQ=255)
                                                        ttGATCACAACTCC‑TTGCTCTGCTGCGTCGGCCTGGGTata                                         >  1:493947/1‑39 (MQ=255)
                                                        ttGATCACAACTCC‑TTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTa                >  1:2728153/1‑66 (MQ=255)
                                                        ttGATCACAACTCC‑TTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTa                >  1:2167257/1‑66 (MQ=255)
                                                           aTCACAACTCCTTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCGGAAg         <  1:1662872/71‑1 (MQ=255)
                                                           aTCACAACTCCTTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCGGAAg         <  1:1144405/71‑1 (MQ=255)
                                                           aTCACAACTCCTTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCGGAAg         <  1:1143000/71‑1 (MQ=255)
                                                             cacaACTCCTTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCGGaa          >  1:1654783/1‑68 (MQ=255)
                                                              acaACTCC‑TTGCTCTGCTGCGTCGGCCTGGGTAATGGTATTTCC‑CCAGTGATTGGCGTACCGGAAGCATTa    >  1:2482711/1‑71 (MQ=255)
                                                              acaACTCC‑TTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTc                                 >  1:427030/1‑43 (MQ=255)
                                                              acaACTCC‑TTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTc                                 >  1:1219768/1‑43 (MQ=255)
                                                                 aCTCC‑TTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCGGAAGc        <  1:628368/65‑1 (MQ=255)
                                                                 aCTCC‑TTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCGGAAGCATTAcc  >  1:1822136/1‑71 (MQ=255)
                                                                  cTCC‑TTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCGGAAGc        <  1:1176687/64‑1 (MQ=255)
                                                                      |                                                                  
CAGAATACGTAATTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCC‑TTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCGGAAGCATTACC  >  W3110S.gb/2866779‑2866914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑