| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,919,937:1 | +C | 81.8% | coding (714/2634 nt) | yebT → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,919,937 | 1 | . | C | 81.8% | 56.9 / 6.6 | 22 | coding (714/2634 nt) | yebT | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (2/2); new base C (10/8); total (12/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGGTAGCCGTTTCTGGAACGTTTCCGGCGTTGATGCCAACGTCAGTATCAGTGGCGCGAAGGT‑G‑AAACTGGAAAGCCTGGCGGCACTGGTTAACGGTGCGATTGCCTTCGATTCACCAGAA > W3110S.gb/1919874‑1919994 | aGGTAGCCGTTTCTGGAACGTTTCCGGCGTTGATGCCAACGTCAGTATCAGTGGCGCGAAGGT‑GCAAACt > 1:309635/1‑70 (MQ=255) gCCGTTTCTGGAACGTTTCCGGCGTTGATGCCAACGTCAGTATCAGTGGCGCGAAGGT‑GCAAACTGGaaa < 1:540927/70‑1 (MQ=255) ccGTTTCTGGAACGTTTCCGGCGTTGATGCCAACGTCAGTATCAGTG‑‑GCGAAGGT‑GCAAACTGGAAAGCCt < 1:2122112/71‑1 (MQ=255) tttCTGGAACGTTTCCGGCGTTGATGCCAACGTCAGTATCAGTGGCGCGAAGGT‑GCAAACTg < 1:36523/62‑1 (MQ=255) tttCTGGAACGTTTCCGGCGTTGATGCCAACGTCAGTATCAGTGGCGCGAAGGT‑GCAAACTg < 1:1985731/62‑1 (MQ=255) tttCTGGAACGTTTCCGGCGTTGATGCCAACGTCAGTATCAGTGGCGCGAAGGT‑GCAAACTg < 1:1876713/62‑1 (MQ=255) tCTGGAACGTTTCCGGAGTTGATGCCAACGTCAGTATCAGTGGCGCGAAtgt‑g > 1:2476420/1‑53 (MQ=255) gTTGATGCCAACGTCAGTATCAGTGGCGCGAAGGT‑G‑AAACTGGAAAGCCTGGCGGCACTGGTTAACGGTGc < 1:133464/71‑1 (MQ=255) gATGCCAACGTCAGTATCAGTGGCGCGAAGGT‑GCAAACTGGAAAGCCTGGCGGCACTGGTTAACGGTGCGa > 1:2473841/1‑71 (MQ=255) gATGCCAACGTCAGTATCAGTGGCGCGAAGGT‑GCAAACTGGAAAGCCTGGCGGCACTGGTTAACGGTGCGa > 1:1492136/1‑71 (MQ=255) gATGCCAACGTCAGTATCAGTGGCGCGAAGGT‑GCAAACTGGAAAGCCTGGCGGCACTGGTTAACGGTGCGa > 1:2653395/1‑71 (MQ=255) gATGCCAACGTCAGTATCAGTGGCGCCAAGGT‑GCAAACTGGAAAGCCTGGCGGCACTGGTTAACgg > 1:1133569/1‑66 (MQ=255) ccAACGTCAGTATCAGTGGCGCGAAGGT‑GCAAACTGGAAAGCCTGGCGGCACt < 1:1430911/53‑1 (MQ=39) tCAGTATCAGTGGCGCGAAGGT‑GCAAACTGGAAAGCCTGGCGGCACTGGTTAACGGTGCGATTGCCTTCGa > 1:1349735/1‑71 (MQ=255) tCAGTATCAGTGGCGCGAAGGT‑GCAAACTGGAAAGCCTGGCGGCACTGGTTAACGGTGCGATTGCCTTCGa > 1:435048/1‑71 (MQ=255) aGTATCAGTGGCGCGAAGGT‑GCAAACTGGAAAGCCTGGCGGCACTGGTTAACGGTGCGa > 1:1756198/1‑59 (MQ=39) aTCAGTGGCGCGAAGGTGG‑AAACTGGAAAGCCTGGCGGCACTGGTTAACGGTGCGATTGCCTTCGATTCAc > 1:289086/1‑71 (MQ=255) tCAGTGGCGCGAAGGT‑GCAAACTGGAAAGCCTGGCGGCACTGGTTAACGGTGCGATTGCCTTCGATTCAc > 1:1778074/1‑70 (MQ=255) tCAGTGGCGCGAAGGT‑G‑AAACTGGAAAGCCTGGCGGCACTGGTTAACGGTGCGATTGCCTTCGATTCAc > 1:175292/1‑69 (MQ=255) aGTGGCGCGAAGGT‑GCAAACTGGAAAGCCTGGCGGCACTGGTTAACGGTGCGATTGCCTTCGATTCAc > 1:816511/1‑68 (MQ=255) aGTGGCGCGAAGGT‑GCAAACTGGAAAGCCTGGCGGCACTGGTTAACGGTGCGATTGCCTTCGATTCACCag < 1:1625003/71‑1 (MQ=255) tGGCGCGAAGGT‑GCAAACTGGAAAGCCTGGCGGCACTGGTTAACGGTGCGATTGCCTTCGATTCACCagaa < 1:322501/71‑1 (MQ=255) aGGT‑G‑AAACTGGAAAGCCTGGCGGCACTGGTTAACGGTGCGATTGCCTTCGAtt < 1:81859/54‑1 (MQ=255) | AGGTAGCCGTTTCTGGAACGTTTCCGGCGTTGATGCCAACGTCAGTATCAGTGGCGCGAAGGT‑G‑AAACTGGAAAGCCTGGCGGCACTGGTTAACGGTGCGATTGCCTTCGATTCACCAGAA > W3110S.gb/1919874‑1919994 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |