| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,997,363:1 | +C | 100% | coding (122/795 nt) | ygeQ ← | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,997,363 | 1 | . | C | 75.0% | 37.7 / 8.7 | 16 | coding (122/795 nt) | ygeQ | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (1/3); new base C (3/9); total (4/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch. | |||||||||||
AGAAGACGATAATTAGGATACTTATCTGGATTTAACGATAAATAACTTTTATCTGTTGCTG‑CCCCCGTGGAATTAAGAATGTTAAATACAGATTCACTCGTCACTTCTGCCCCTGTC > W3110S.gb/2997303‑2997419 | agaagaCGATAATTAGGATACTTATCTGGATTTAACGATAAATAACTTTTATCTGTTGCTG‑CCCCCGTgg > 1:347364/1‑70 (MQ=255) cGATAATTAGGATACTTATCTGGATTTAACGATAAATAACTTTTATCTGTTGCTG‑CCCCCGTGGAATTAAg < 1:1082108/71‑1 (MQ=255) aTCTGGATTTAACGATAAATAACTTTTATCTGTTGCTGCCCCCCGTGGAATTAAGAATGTTAAATACAGa < 1:1308637/70‑1 (MQ=255) aTCTGGATTTAACGATAAATAACTTTTATCTGTTGCTGCCCCCCGTGGAATTAAGAATGTTAAATACAGa < 1:153950/70‑1 (MQ=255) aTCTGGATTTAACGATAAATAACTTTTATCTGTTGCTGCCCCCCGTGGAATTAAGAATGTTAAATACAGa < 1:1661665/70‑1 (MQ=255) aTCTGGATTTAACGATAAATAACTTTTATCTGTTGCTGCCCCCCGTGGAATTAAGAATGTTAAATACAGa < 1:1828607/70‑1 (MQ=255) aTCTGGATTTAACGATAAATAACTTTTATCTGTTGCTGCCCCCCGTGGAATTAAGAATGTTAAATACAGa < 1:832541/70‑1 (MQ=255) gATTTAACGATAAATAACTTTTATCTGTTGCTG‑CCCCCGTGGAATTAAGAATGTTAAATACAGATTCa < 1:196476/68‑1 (MQ=255) aTTTAACGATAAATAACTTTTATCTGTTGCTG‑CCCCCGTGGAATTAAGAATGTTAAATACAGATTCa < 1:1321155/67‑1 (MQ=255) tAACGATAAATAACTTTTATCTGTTGCTGCCCCCCGTGGAATTAAGAATGTTAAATACAGATTCACTCGt < 1:1863050/70‑1 (MQ=255) aCGATAAATAACTTTTATCTGTTGCTGCCCCCCGTGGAATTAAGAATGTTAAATACAGATTCACTCGTCAc < 1:107043/71‑1 (MQ=255) aCGATAAATAACTTTTATCTGTTGCTGCCCCCCGTGGAATTAAGAATGTTAAATACAGATTCACTCGTCAc < 1:701885/71‑1 (MQ=255) aataaCTTTTATCTGTTGCTGCCCCCCGTGGAATTAAGAATGTTAAATACAGATTCACTCGTCACTTCTGc < 1:1650881/71‑1 (MQ=255) tttATCTGTTGCTGCCCCCCGTGGAATTAAGAATGTTAAATACAGATTCACTCGTCACTTCTGCCCCTGt > 1:1812673/1‑70 (MQ=255) tttATCTGTTGCTGCCCCCCGTGGAATTAAGAATGTTAAATACAGATTCACTCGTCACTTCTGCCCCTGt > 1:831032/1‑70 (MQ=255) tttATCTGTTGCTGCCCCCCGTGGAATTAAGAATGTTAAATACAGATTCACTCGTCACTTCTGCCCCTGTc > 1:419887/1‑71 (MQ=255) | AGAAGACGATAATTAGGATACTTATCTGGATTTAACGATAAATAACTTTTATCTGTTGCTG‑CCCCCGTGGAATTAAGAATGTTAAATACAGATTCACTCGTCACTTCTGCCCCTGTC > W3110S.gb/2997303‑2997419 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |