Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	712,019	G→A	intergenic (‑132/+8)	fldA ← / ← ybfE	flavodoxin 1/LexA regulated protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	712,019	0	G	A	100.0%	167.4 / NA	53	intergenic (‑132/+8)	fldA/ybfE	flavodoxin 1/LexA regulated protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (46/7);  total (46/7)

CAGTCTTGCGGAATCAGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACATCTC  >  W3110S.gb/711967‑712077                                                     |                                                           cAGTCTTGCGGAATCAGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAATGGAAATTTaa                                                 >  1:1789382/1‑64 (MQ=255) cAGTCTTGCGGAATCAGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAATGGAAATTTaa                                                 >  1:2768035/1‑64 (MQ=255)                  cAAACGAGCAGGACTGCACACTGTGCTACATGAAAATGGAAATTTAAACGATGCCCTGACTACg                                <  1:793318/64‑1 (MQ=255)                  cAAACGAGCAGGACTGCACACTGTGCTACATGAAAATGGAAATTTAAACGATGCCCTGACTACg                                <  1:1839217/64‑1 (MQ=255)                  cAAACGAGCAGGACTGCACACTGTGCTACATGAAAATGGAAATTTAAACGATGCCCTGACTACg                                <  1:688645/64‑1 (MQ=255)                  cAAACGAGCAGGACTGCACACTGTGCTACATGAAAATGGAAATTTAAACGATGCCCTGACTACg                                <  1:1610988/64‑1 (MQ=255)                  cAAACGAGCAGGACTGCACACTGTGCTACATGAAAATGGAAATTTAAACGATGCCCTGACTACg                                <  1:3104305/64‑1 (MQ=255)                  cAAACGAGCAGGACTGCACACTGTGCTACATGAAAATGGAAATTTAAACGATGCCCTGACTACg                                <  1:2210597/64‑1 (MQ=255)                  cAAACGAGCAGGACTGCACACTGTGCTACATGAAAATGGAAATTTAAACGATGCCCTGACTACg                                <  1:2404923/64‑1 (MQ=255)                                      cTGTGCTACATGAAAATGGAAGTTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCa            >  1:430940/1‑64 (MQ=255)                                      cTGTGCTACATGAAAATGGAAATTTAAACGATGCCCt                                       >  1:1440538/1‑37 (MQ=255)                                      cTGTGCTACATGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCa            >  1:1154555/1‑64 (MQ=255)                                      cTGTGCTACATGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCa            >  1:2821125/1‑64 (MQ=255)                                      cTGTGCTACATGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCa            >  1:2878938/1‑64 (MQ=255)                                      cTGTGCTACATGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCa            >  1:2067499/1‑64 (MQ=255)                                      cTGTGCTACATGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCa            >  1:37796/1‑64 (MQ=255)                                      cTGTGCTACATGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCa            >  1:2002442/1‑64 (MQ=255)                                      cTGTGCTACATGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCa            >  1:610492/1‑64 (MQ=255)                                      cTGTGCTACATGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCa            >  1:713922/1‑64 (MQ=255)                                      cTGTGCTACATGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCa            >  1:1834767/1‑64 (MQ=255)                                      cTGTGCTACATGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCa            >  1:1820513/1‑64 (MQ=255)                                      cTGTGCTACATGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCa            >  1:1079417/1‑64 (MQ=255)                                      cTGTGCTACATGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCa            >  1:1226118/1‑64 (MQ=255)                                      cTGTGCTACATGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCa            >  1:1421601/1‑64 (MQ=255)                                      cTGTGCTACATGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCa            >  1:1472579/1‑64 (MQ=255)                                      cTGTGCTACATGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCAAGTTGCTGCa            >  1:2565536/1‑64 (MQ=255)                                      cTGTGCTACATGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCAGCCAGTtgctgc             >  1:521159/1‑63 (MQ=255)                                               aTGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGcgccgc                       >  1:1799103/1‑44 (MQ=255)                                               aTGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACAtctc  >  1:1412181/1‑65 (MQ=255)                                               aTGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACAtctc  >  1:970051/1‑65 (MQ=255)                                               aTGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACAtctc  >  1:1869645/1‑65 (MQ=255)                                               aTGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACAtctc  >  1:1910892/1‑65 (MQ=255)                                               aTGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACAtctc  >  1:1245108/1‑65 (MQ=255)                                               aTGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACAtctc  >  1:1248670/1‑65 (MQ=255)                                               aTGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACAtctc  >  1:192836/1‑65 (MQ=255)                                               aTGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACAtctc  >  1:453144/1‑65 (MQ=255)                                               aTGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACAtctc  >  1:1288257/1‑65 (MQ=255)                                               aTGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACAtctc  >  1:415944/1‑65 (MQ=255)                                               aTGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACAtctc  >  1:1311473/1‑65 (MQ=255)                                               aTGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACAtctc  >  1:1408328/1‑65 (MQ=255)                                               aTGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACAtctc  >  1:3103101/1‑65 (MQ=255)                                               aTGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACAtctc  >  1:2098368/1‑65 (MQ=255)                                               aTGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACAtctc  >  1:1628466/1‑65 (MQ=255)                                               aTGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACAtctc  >  1:2802929/1‑65 (MQ=255)                                               aTGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACAtctc  >  1:278447/1‑65 (MQ=255)                                               aTGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACAtctc  >  1:1582860/1‑65 (MQ=255)                                               aTGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACAtctc  >  1:2699556/1‑65 (MQ=255)                                               aTGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACAtctc  >  1:2670563/1‑65 (MQ=255)                                               aTGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACAtctc  >  1:2094178/1‑65 (MQ=255)                                               aTGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACAtctc  >  1:1520612/1‑65 (MQ=255)                                               aTGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACAtctc  >  1:2396189/1‑65 (MQ=255)                                               aTGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACAtctc  >  1:1540271/1‑65 (MQ=255)                                               aTGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACAtctc  >  1:2102359/1‑65 (MQ=255)                                                     |                                                           CAGTCTTGCGGAATCAGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACATCTC  >  W3110S.gb/711967‑712077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑