Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,330,087	(G)5→4	coding (1270/1434 nt)	dacB →	D‑alanyl‑D‑alanine carboxypeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,330,083	0	G	.	100.0%	148.0 / NA	37	coding (1266/1434 nt)	dacB	D‑alanyl‑D‑alanine carboxypeptidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (16/21);  total (16/21)

TGCAGTACCGTGCAGGTCTGCATCAGGCGGGCGTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCAGGGGGTATATAACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATA  >  W3110S.gb/3330013‑3330148                                                                       |                                                                  tGCAGTACCGTGCAGGTCTGCATCAGGCGGGCGTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCAg                                                                   >  1:3446352/1‑71 (MQ=255) tGCAGTACCGTGCAGGTCTGCATCAGGCGGGCGTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCAg                                                                   >  1:3440781/1‑71 (MQ=255)    aGTACCGTGCAGGTCTGCATCAGGCGGGCGTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCAgggg                                                                <  1:677167/71‑1 (MQ=255)    aGTACCGTGCAGGTCTGCATCAGGCGGGCGTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCAgggg                                                                <  1:3456750/71‑1 (MQ=255)      tACCGTGCAGGTCTGCATCAGGCGGGCGTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCTggggta                                                              <  1:593940/71‑3 (MQ=255)      tACCGTGCAGGTCTGCATCAGGCGGGCGTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCAggggta                                                              <  1:471403/71‑3 (MQ=255)          gTGCAGGTCTGCATCAGGCGGGCGTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCA‑GGGGtatata                                                         <  1:1710023/71‑1 (MQ=255)             cAGGTCTGCATCAGGCGGGCGTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCA‑GGGGTATATAAcc                                                      >  1:1753458/1‑71 (MQ=255)             cAGGTCTGCATCAGGCGGGCGTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCA‑GGGGTATATAAcc                                                      >  1:4284963/1‑71 (MQ=255)             cAGGTCTGCATCAGGCGGGCGTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCA‑GGGGTATATAAc                                                       <  1:2841650/70‑1 (MQ=255)                  cTGCATCAGGCGGGCGTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCA‑GGGGTATATAACCTGGCg                                                 >  1:810294/1‑71 (MQ=255)                  cTGCATCAGGCGGGCGTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCA‑GGGGTATATAACCTGGCg                                                 >  1:763440/1‑71 (MQ=255)                  cTGCATCAGGCGGGCGTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCA‑GGGGTATATAACCTGGCg                                                 >  1:4183071/1‑71 (MQ=255)                  cTGCATCAGGCGGGCGTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCA‑GGGGTATATAACCTGGCg                                                 >  1:2317923/1‑71 (MQ=255)                   tGCATCAGGCGGGCGTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCA‑GGGGtatata                                                         <  1:1210607/62‑1 (MQ=255)                     cATCAGGCGGGCGTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCAgggg                                                                >  1:3569109/1‑54 (MQ=255)                       tCAGGCGGGCGTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCAg                                                                   <  1:4427536/49‑1 (MQ=255)                       tCAGGCGGGCGTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCAg                                                                   <  1:3709727/49‑1 (MQ=255)                       tCAGGCGGGCGTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCA‑GGGGTATATAACCTGGCGGGAtt                                            <  1:167788/71‑1 (MQ=255)                       tCAGGCGGGCGTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCA‑GGGGTATATAACCTGGCGGGAtt                                            <  1:361829/71‑1 (MQ=255)                       tCAGGCGGGCGTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCA‑GGGGTATATAACCTGGCGGGAtt                                            <  1:3889751/71‑1 (MQ=255)                        cAGGCGGGCGTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCA‑GGGGTATATAACCTGGCGGGAtt                                            >  1:3999332/1‑70 (MQ=255)                         aGGCGGGCGTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCA‑GGGGTATATAACCTGGCGGGATTc                                           <  1:1523793/70‑1 (MQ=255)                         aGGCGGGCGTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCA‑GGGGTATATAACCTGGCGGGATTc                                           <  1:4325535/70‑1 (MQ=255)                         aGGCGGGCGTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCA‑GGGGTATATAACCTGGCGGGATTCa                                          >  1:3944524/1‑71 (MQ=255)                          ggcgggcgTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCA‑GGGGTATATAACCTGGCGGGATTCAt                                         <  1:4597545/71‑1 (MQ=255)                          ggcgggcgTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCA‑GGGGTATATAACCTGGCGGGATTCAt                                         <  1:4295362/71‑1 (MQ=255)                          ggcgggcgTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCA‑GGGGTATATAACCTGGCGGGATTCAt                                         <  1:393799/71‑1 (MQ=255)                              ggcgTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCAgggg                                                                <  1:3101290/45‑1 (MQ=255)                                cgTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCA‑GGGGTATATAACCTGGCGGGATTCATTACca                                    >  1:2242588/1‑70 (MQ=255)                                    gatggaAAAGTCTCAGCGAAAACCGGTTCGTTGCA‑GGGGTATATAACCTGGCGGGAtt                                            >  1:3339142/1‑58 (MQ=255)                                    gatggaAAAGTCTCAGCGAAAACCGGTTCGTTGCA‑GGGGTATATAACCTGGCGGGATTCATTACCACagcg                               <  1:1014817/71‑1 (MQ=255)                                     atggaAAAGTCTCAGCGAAAACCGGTTCGTTGCA‑GGGGTATATAACCTGGCGGGATTCATTACCACagcga                              <  1:1603713/71‑1 (MQ=255)                                       ggaAAAGTCTCAGCGAAAACCGGTTCGTTGCA‑GGGGTATATAACCTGGCGGGATTCATTACCACagcgagc                            >  1:4173600/1‑71 (MQ=255)                                          agaGTCTCAGCGAAAACCGGTTCGTTGCA‑GGGGTATATAACCTGGCGGGATTCATTACca                                    >  1:463377/3‑60 (MQ=255)                                          aaaGTCTCAGCGAAAACCGGTTCGTTGCA‑GGGGTATATAACCTGGCGGGATTCATTACca                                    >  1:1045392/1‑60 (MQ=255)                                          aaaGTCTCAGCGAAAACCGGTTCGTTGCA‑GGGGTATATAACCTGGCGGGATTCATTACca                                    >  1:1464314/1‑60 (MQ=255)                                          aaaGTCTCAGCGAAAACCGGTTCGTTGCA‑GGGGTATATAACCTGGCGGGATTCATTACca                                    >  1:2376626/1‑60 (MQ=255)                                           aaGTCTCAGCGAAAACCGGTTCGTTGCA‑GGGGTATATAACCTGGCGGGATTCATTACCACAGCGAGCGGGc                        <  1:2259506/71‑1 (MQ=255)                                                tcAGCGAAAACCGGTTCGTTGCA‑GGGGTATATAACCTGGCGGGATTc                                           <  1:2538892/47‑1 (MQ=39)                                                  aGCGAAAACCGGTTCGTTGCA‑GGGGTATATAACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACg                    <  1:2258143/68‑1 (MQ=255)                                                  aGCGAAAACCGGTTCGTTGCA‑GGGGTATATAACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACg                    <  1:3838303/68‑1 (MQ=255)                                                  aGCGAAAACCGGTTCGTTGCA‑GGGGTATATAACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACg                    <  1:408901/68‑1 (MQ=255)                                                     gAAAACCGGTTCGTTGCA‑GGGGTATATAACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGc              >  1:4209147/1‑71 (MQ=255)                                                             gTTCGTTGCA‑GGGGTATATAACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGc      <  1:3821060/71‑1 (MQ=255)                                                                 gTTGCA‑GGGGTATATAACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAata  <  1:3459061/71‑1 (MQ=255)                                                                  ttGCA‑GGGGTATATAACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCaa    <  1:1862508/68‑1 (MQ=255)                                                                       |                                                                  TGCAGTACCGTGCAGGTCTGCATCAGGCGGGCGTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCAGGGGGTATATAACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATA  >  W3110S.gb/3330013‑3330148

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑