| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 3,514,320 | (C)7→6 | intergenic (+19/‑74) | ftsN → / → hslV | essential cell division protein/peptidase component of the HslUV protease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,514,314 | 0 | C | . | 97.8% | 176.7 / ‑2.2 | 46 | intergenic (+13/‑80) | ftsN/hslV | essential cell division protein/peptidase component of the HslUV protease |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base . (15/30); minor base A (0/1); total (15/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
CAATCGGTTGAAGATGGCGGGTCATACAAACTGCATTCGGCTCGCCGCC‑GGGGGTTGAAACCCTCAAAATCCCCCCCATCTATAATTGCATTATGCCCCGTACTTTTGTACGGGGTTTGTACTCTGTA > W3110S.gb/3514244‑3514371 | cAATCGGTTGAAGATGGCGGGTCATACAAACTGCATTCGGCTCGCCGCC‑GGGGGTTGAAACCCTCAAAATc > 1:1734500/1‑71 (MQ=255) gAAGATGGCGGGTCATACAAACTGCATTCGGCTCGCCGCC‑GGGGGTTGAAACCCTCAAAATccccccatc < 1:4456011/70‑4 (MQ=255) gATGGCGGGTCATACAAACTGCATTCGGCTCGCCGCC‑GGGGGTTGAAACCCTCAAAAT‑CCCCCCATCtata < 1:784447/71‑1 (MQ=255) gATGGCGGGTCATACAAACTGCATTCGGCTCGCCGCC‑GGGGGTTGAAACCCTCAAAAT‑CCCCCCATCtata < 1:2179/71‑1 (MQ=255) gCGGGTCATACAAACTGCATTCGGCTCGCCGCC‑GGGGGTTGAAACCCTCAAAAT‑CCCCCCATCTATAATTg < 1:3982880/71‑1 (MQ=255) gggcATACAAACTGCATTCGGCTCGCCGCC‑GGGGGTTGAAACCCTCAAAAT‑CCCCCCATCTATAATTGCAt < 1:3696864/68‑1 (MQ=255) ggTCATACAAACTGCATTCGGCTCGCCGCC‑GGGGGTTGAAACCCTCAAAAT‑CCCCCCATCTATAATTGCAt < 1:1032886/71‑1 (MQ=255) ggTCATACAAACTGCATTCGGCTCGCCGCC‑GGGGGTTGAAACCCTCAAAAT‑CCCCCCATCTATAATTGCAt < 1:3112049/71‑1 (MQ=255) ggTCATACAAACTGCATTCGGCTCGCCGCC‑GGGGGTTGAAACCCTCAAAAT‑CCCCCCATCTATAATTGCAt < 1:278236/71‑1 (MQ=255) ggTCATACAAACTGCATTCGGCTCGCCGCC‑GGGGGTTGAAACCCTCAAAAT‑CCCCCCATCTATAATTGCAt < 1:2754947/71‑1 (MQ=255) ggTCATACAAACTGCATTCGGCTCGCCGCC‑GGGGGTTGAAACCCTCAAAAT‑CCCCCCATCTATAATTGCAt < 1:4588372/71‑1 (MQ=255) ggTCATACAAACTGCATTCGGCTCGCCGCC‑GGGGGTTGAAACCCTCAAAAT‑CCCCCCATCTATAATTGCAt < 1:1641777/71‑1 (MQ=255) gTCATACAAACTGCATTCGGCTCGCCGCC‑GGGGGTTGAAACCCTCAAAAT‑CCCCCCATCTATAATTGCAt < 1:696635/70‑1 (MQ=255) tCATACAAACTGCATTCGGCTCGCCGCC‑GGGGGTTGAAACCCTCAAAAT‑CCCCCCATCTATAATTGCATTa > 1:166611/1‑71 (MQ=255) tCATACAAACTGCATTCGGCTCGCCGCC‑GGGGGTTGAAACCCTCAAAAT‑CCCCCCATCTATAATTGCATTa > 1:2786570/1‑71 (MQ=255) aTACAAACTGCATTCGGCTCGCCGCC‑GGGGGTTGAAACCCTCAAAAT‑CCCCCCATCTATAATTGCATTATg < 1:2845801/71‑1 (MQ=255) tACAAACTGCATTCGGCTCGCCGCC‑GGGGGTTGAAACCCTCACAAT‑CCCCCCATCTATAATTGCAt < 1:741561/66‑1 (MQ=255) tACAAACTGCATTCGGCTCGCCGCC‑GGGGGTTGAAACCCTCAAAAT‑CCCCCCATCTATAATTGCAt < 1:2447453/66‑1 (MQ=255) tACAAACTGCATTCGGCTCGCCGCC‑GGGGGTTGAAACCCTCAAAAT‑CCCCCCATCTATAATTGCAt < 1:2747770/66‑1 (MQ=255) tACAAACTGCATTCGGCTCGCCGCC‑GGGGGTTGAAACCCTCAAAAT‑CCCCCCATCTATAATTGCAt < 1:746853/66‑1 (MQ=255) tACAAACTGCATTCGGCTCGCCGCC‑GGGGGTTGAAACCCTCAAAAT‑CCCCCCATCTATAATTGCAt < 1:3493145/66‑1 (MQ=255) tACAAACTGCATTCGGCTCGCCGCC‑GGGGGTTGAAACCCTCAAAAT‑CCCCCCATCTATAATTGCAt < 1:3388195/66‑1 (MQ=255) tACAAACTGCATTCGGCTCGCCGCC‑GGGGGTTGAAACCCTCAAAA‑ACCCCCAATCTATAATTGCAt < 1:2316514/66‑1 (MQ=255) aCAAACTGCATTCGGCTCGCCGCC‑GGGGGTTGAAACCCTCAAAAT‑CCCCCCATCTATAATTGCATTa > 1:1272374/1‑67 (MQ=255) cAAACTGCATTCGGCTCGCCGCCGGGGGGTTGAAACCCTCAAAAT‑CCCCCCATCTATAATTGCAt > 1:2289944/1‑65 (MQ=255) cAAACTGCATTCGGCTCGCCGCC‑GGGGGTTGAAACCCTCAAAATc > 1:1665052/1‑45 (MQ=255) cAAACTGCATTCGGCTCGCCGCC‑GGGGGTTGAAACCCTCAAAAT‑CCCCCCATCTATAATTGCATTa > 1:1533990/1‑66 (MQ=255) cAAACTGCATTCGGCTCGCCGCC‑GGGGGTTGAAACCCTCAAAAT‑CCCCCCATCTATAATTGCATTa > 1:2067131/1‑66 (MQ=255) cAAACTGCATTCGGCTCGCCGCC‑GGGGGTTGAAACCCTCAAAAT‑CCCCCCATCTATAATTGCATTATGccc > 1:3294296/1‑71 (MQ=255) cAAACTGCATTCGGCTCGCCGCC‑GGGGGTTGAAACCCTCAAAAT‑CCCCCCATCTATAATTGCATTATGccc > 1:870771/1‑71 (MQ=255) cAAACTGCATTCGGCTCGCCGCC‑GGGGGTTGAAACCCTCAAAAT‑CCCCCCATCTATAATTGCATTATGccc > 1:4049857/1‑71 (MQ=255) cAAACTGCATTCGGCTCGCCGCC‑GGGGGTTGAAACCCTCAAAAT‑CCCCCCATCTATAATTGCATTATGccc > 1:4061674/1‑71 (MQ=255) cAAACTGCATTCGGCTCGCCGCC‑GGGGGTTGAAACCCTCAAAAT‑CCCCCCATCTATAATTGCATTATGccc > 1:4287921/1‑71 (MQ=255) cAAACTGCATTCGGCTCGCCGCC‑GGGGGTTGAAACCCTCAAAAT‑CCCCCCATCTATAATTGCATTATGccc > 1:4371852/1‑71 (MQ=255) cGGCTCGCCGCC‑GGGGGTTGAAACCCTCAAAAT‑CCCCCCATCTATAATTGCATTATGCCCCGTACTTTTg > 1:4438020/1‑70 (MQ=255) ggCTCGCCGCC‑GGGGGTTGAAACCCTCAAAAT‑CCCCCCATCtat < 1:4601180/44‑1 (MQ=255) gc‑gGGGGGTGAAACCCTCAAAAT‑CCCCCCATCTATAATTGCAt < 1:3408220/42‑1 (MQ=255) cc‑gGGGGTTGAAACCCTCAAAAT‑CCCCCCATCTATAATTGCATTATGCCCCGTACTTTTGTACGGGGTTTg < 1:3202348/71‑1 (MQ=255) ttGAAACCCTCAAAAT‑CCCCCCATCTATAATTGCAt < 1:422583/36‑1 (MQ=38) ttGAAACCCTCAAAAT‑CCCCCCATCTATAATTGCAt < 1:3853145/36‑1 (MQ=38) ttGAAACCCTCAAAAT‑CCCCCCATCTATAATTGCAt < 1:159851/36‑1 (MQ=38) ttGAAACCCTCAAAAT‑CCCCCCATCTATAATTGCAt < 1:703096/36‑1 (MQ=38) ttGAAACCCTCAAAAT‑CCCCCCATCTATAATTGCAt < 1:3471757/36‑1 (MQ=38) ttGAAACCCTCAAAAT‑CCCCCCATCTATAATTGCATTATGCCCCGTACTTTTGTACGGGGTTTGTACTCTg < 1:25707/71‑1 (MQ=255) ttGAAACCCTCAAAAT‑CCCCCCATCTATAATTGCATTATGCCCCGTACTTTTGTACGGGGTTTGTACTCTg < 1:1580043/71‑1 (MQ=255) ttGAAACCCTCAAAAT‑CCCCCCATCTATAATTGCATTATGCCCCGTACTTTTGTACGGGGTTTGTACTCTg < 1:1291271/71‑1 (MQ=255) ttGAAACCCTCAAAAT‑CCCCCCATCTATAATTGCATTATGCCCCGTACTTTTGTACGGGGTTTGTACTCTg < 1:947989/71‑1 (MQ=255) gAAACCCTCAAAAT‑CCCCCCATCTATAATTGCATTATGCCCCGTACTTTTGTACGGGGTTTGTACTCTGTa > 1:450422/1‑71 (MQ=255) gAAACCCTCAAAAT‑CCCCCCATCTATAATTGCATTATGCCCCGTACTTTTGTACGGGGTTTGTACTCTGTa > 1:4541448/1‑71 (MQ=255) | CAATCGGTTGAAGATGGCGGGTCATACAAACTGCATTCGGCTCGCCGCC‑GGGGGTTGAAACCCTCAAAATCCCCCCCATCTATAATTGCATTATGCCCCGTACTTTTGTACGGGGTTTGTACTCTGTA > W3110S.gb/3514244‑3514371 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |