Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	993,711	Δ1 bp	coding (756/768 nt)	ssuB ←	alkanesulfonate transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	993,708	0	T	.	100.0%	59.0 / NA	16	coding (759/768 nt)	ssuB	alkanesulfonate transporter subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (12/4);  total (12/4)

TGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGC  >  W3110S.gb/993669‑993771                                        |                                                                tGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTG‑TTTCTCAGGCGAGTTTCAGATTCACCACGTTg                                 <  1:849974/71‑1 (MQ=255)         aTAGCGCCGCCATTCGGGGAATTTACCCCTG‑TTTCTCAGGCGAGTTTCAGATTcacca                                      >  1:4197343/1‑58 (MQ=255)         aTAGCGCCGCCATTCGGGGAATTTACCCCTG‑TTTCTCAGGCGAGTTTCAGATTCACCACGTTGc                                <  1:3198924/64‑1 (MQ=255)           aGCGCCGCCATTCGGGGAATTTACCCCTG‑TTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCa                            >  1:4319977/1‑66 (MQ=255)           aGCGCCGCCATTCGGGGAATTTACCCCTG‑TTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGt                       >  1:4273402/1‑71 (MQ=255)           aGCGCCGCCATTCGGGGAATTTACCCCTG‑TTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGt                       >  1:4494057/1‑71 (MQ=255)            gcgcCGCCATTCGGGGAATTTACCCCTG‑TTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGtt                      >  1:2176491/1‑71 (MQ=255)            gcgcCGCCATTCGGGGAATTTACCCCTG‑TTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGtt                      >  1:3113002/1‑71 (MQ=255)            gcgcCGCCATTCGGGGAATTTACCCCTG‑TTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGtt                      >  1:943030/1‑71 (MQ=255)                       cGGGGAATTTAACCCTG‑TTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTcc           >  1:2004411/1‑71 (MQ=255)                           gAATTTACCCCTG‑TTTCTCAGGCGAGTTTCAGATTcaccac                                     <  1:2901275/41‑1 (MQ=39)                              tttACCCCTG‑TTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCAcc                          <  1:3656305/49‑1 (MQ=255)                                tACCCCTG‑TTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCa                            >  1:4662199/1‑45 (MQ=255)                                tACCCCTG‑TTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCa    >  1:4129119/1‑69 (MQ=255)                                tACCCCTG‑TTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGc  >  1:1469879/1‑71 (MQ=255)                                tACCCATG‑TTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGc  >  1:758226/1‑71 (MQ=255)                                        |                                                                TGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGC  >  W3110S.gb/993669‑993771

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑