Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,027,474	(T)7→6	intergenic (+124/‑174)	yodD → / → yedP	hypothetical protein/conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,027,468	0	T	.	100.0%	262.3 / NA	65	intergenic (+118/‑180)	yodD/yedP	hypothetical protein/conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (24/41);  total (24/41)

CAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGA  >  W3110S.gb/2027405‑2027534                                                                |                                                                   cAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAAttttttcg                                                             >  1:1801490/1‑69 (MQ=255) cAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAAttttttcg                                                             >  1:2536648/1‑69 (MQ=255)     aagCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGa                                                        >  1:2781687/1‑71 (MQ=255)       gCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGAc                                                       >  1:1134528/1‑70 (MQ=255)            tAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGaa                                                  >  1:4423233/1‑70 (MQ=255)            tAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGa                                                   >  1:4095398/1‑69 (MQ=255)              aaaaaTTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGaaaa                                                <  1:4663613/70‑1 (MQ=255)               aaaaTTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAata                                              <  1:4302933/71‑1 (MQ=255)                   ttCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGaaaa                                                <  1:3961883/65‑1 (MQ=255)                     cGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGc                                         <  1:4049668/70‑1 (MQ=255)                     cGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGc                                         >  1:3657044/1‑70 (MQ=255)                      gTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCa                                       <  1:4182161/71‑1 (MQ=255)                      gTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCa                                       <  1:4301798/71‑1 (MQ=255)                      gTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCa                                       <  1:3807127/71‑1 (MQ=255)                       ttACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCat                                      <  1:784901/71‑1 (MQ=255)                         acacCAGGAAATCTGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCatat                                    >  1:1675627/1‑71 (MQ=255)                         acacCAGGAAATCTGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCatat                                    >  1:2535173/1‑71 (MQ=255)                         acacCAGGAAATCTGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCatat                                    >  1:3428253/1‑71 (MQ=255)                          cacCAGGAAATCTGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATatg                                   <  1:3549993/71‑1 (MQ=255)                          cacCAGGAAATCTGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATatg                                   <  1:2188064/71‑1 (MQ=255)                          cacCAGGAAATCTGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATatg                                   <  1:708239/71‑1 (MQ=255)                           acCAGGAAATCTGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGaaa                                                 <  1:4342962/56‑1 (MQ=255)                           acCAGGAAATCTGATGTGTTCATCACCTTATCCGCAA‑TTTATTCGCTGACAAGaaa                                                 <  1:4070657/56‑1 (MQ=255)                             cAGGAAATCTGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATatgaat                                <  1:2568086/71‑1 (MQ=255)                                 aaaTCTGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGAtt                            <  1:2205314/71‑1 (MQ=255)                                 aaaTCTGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGAtt                            <  1:4072375/71‑1 (MQ=255)                                 aaaTCTGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGAtt                            <  1:2011141/71‑1 (MQ=255)                                 aaaTCTGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGAtt                            <  1:389613/71‑1 (MQ=255)                                 aaaTCTGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGAtt                            <  1:1741143/71‑1 (MQ=255)                                  aaTCTGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGAtt                            >  1:2126992/1‑70 (MQ=255)                                  aaTCTGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGAtt                            >  1:1252494/1‑70 (MQ=255)                                  aaTCTGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTa                           <  1:1062262/71‑1 (MQ=255)                                   aTCTGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGaaaa                                                <  1:3449914/49‑1 (MQ=39)                                    tCTGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGaaaa                                                <  1:2793689/48‑1 (MQ=39)                                    tCTGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAata                                              <  1:3834779/50‑1 (MQ=39)                                      tGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATAtt                                            >  1:444521/1‑50 (MQ=39)                                      tGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTaa                          >  1:4369695/1‑68 (MQ=255)                                      tGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTaa                          >  1:1768848/1‑68 (MQ=255)                                        aTGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCt                      <  1:3316345/70‑1 (MQ=255)                                           tgtTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGaaaa                                                <  1:4316497/41‑1 (MQ=38)                                           tgtTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACt                   <  1:3473187/70‑1 (MQ=255)                                           tgtTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACt                   <  1:3760310/70‑1 (MQ=255)                                           tgtTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACt                   <  1:1345544/70‑1 (MQ=255)                                           tgtTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGACATATGAATGATTAAGGCTACt                   >  1:2565467/1‑70 (MQ=255)                                             ttCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCa                                       <  1:461237/48‑1 (MQ=39)                                             ttCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATatgaa                                 >  1:3257731/1‑54 (MQ=255)                                             ttCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATatgaa                                 >  1:3190581/1‑54 (MQ=255)                                             ttCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATatg                                   <  1:1838509/52‑1 (MQ=255)                                             ttCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTAt                >  1:659190/1‑71 (MQ=255)                                               catcaCCTTATCCGCAA‑TTTTTTCGCTGACAAGaaaa                                                <  1:2379850/37‑1 (MQ=37)                                                   aCCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCg                                          >  1:4656529/1‑39 (MQ=37)                                                   aCCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGaa             >  1:926730/1‑68 (MQ=255)                                                   aCCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGaa             >  1:2946565/1‑68 (MQ=255)                                                   aCCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGaa             >  1:1863257/1‑68 (MQ=255)                                                   aCCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGaa             >  1:153493/1‑68 (MQ=255)                                                    ccTTATCCTCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGa         <  1:1100596/71‑1 (MQ=255)                                                    ccTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGa         <  1:3853313/71‑1 (MQ=255)                                                    ccTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGa         <  1:1560017/71‑1 (MQ=255)                                                     cTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTaa                          <  1:2270990/53‑1 (MQ=255)                                                        aTCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGaaa       <  1:3326052/69‑1 (MQ=255)                                                         tCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAAtgt    <  1:261354/71‑1 (MQ=255)                                                         tCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAAtgt    <  1:164513/71‑1 (MQ=255)                                                         tCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTACGAATAGAAAtgt    <  1:3009932/71‑1 (MQ=255)                                                           cGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAAtgtg   <  1:4053169/70‑1 (MQ=255)                                                           cGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAAtgtg   <  1:2978479/70‑1 (MQ=255)                                                           cGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGa  >  1:2708140/1‑71 (MQ=255)                                                           cGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGa  >  1:702121/1‑71 (MQ=255)                                                                |                                                                   CAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGA  >  W3110S.gb/2027405‑2027534

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑