Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,369,156	(T)6→7	coding (178/465 nt)	yhcH ←	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,369,150	1	.	T	100.0%	229.0 / NA	76	A62T (GCG→ACG)	yhcH	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (36/40);  total (36/40)

TGCCAAACAGAATCCGTTCCTCACCGTTTAATAACAGCTGGATATCAATGTATTGCTCGTGCAATTCCGC‑‑TTTTTTCTCGACGGGCGATTGAGTGTTAAACGTCATGACATTCATAAAGATATTGTCGCCCTGT  >  W3110S.gb/3369081‑3369214                                                                       |                                                                  tgccaAACAGAATCCGTTCCTCACCGTTTAATAACAGCTGGATATCAATGTATTGCTCGTGCAATTCCGC‑‑t                                                                 <  1:1021098/71‑1 (MQ=255)   ccaAACAGAATCCGTTCCTCACCGTTTAATAACAGCTGGATATCAATGTATTGCTCGTGCAATTCCGC‑‑ttt                                                               >  1:194094/1‑71 (MQ=255)   ccaAACAGAATCCGTTCCTCACCGTTTAATAACAGCTGGATATCAATGTATTGCTCGTGCAATTCCGC‑‑ttt                                                               >  1:2021843/1‑71 (MQ=255)   ccaAACAGAATCCGTTCCTCACCGTTTAATAACAGCTGGATATCAATGTATTGCTCGTGCAATTCCGC‑‑ttt                                                               >  1:2192511/1‑71 (MQ=255)   ccaAACAGAATCCGTTCCTCACCGTTTAATAACAGCTGGATATCAATGTATTGCTCGTGCAATTCCGC‑‑ttt                                                               >  1:2812530/1‑71 (MQ=255)   ccaAACAGAATCCGTTCCTCACCGTTTAATAACAGCTGGATATCAATGTATTGCTCGTGCAATTCCGC‑‑ttt                                                               >  1:3462362/1‑71 (MQ=255)   ccaAACAGAATCCGTTCCTCACCGTTTAATAACAGCTGGATATCAATGTATTGCTCGTGCAATTCCGC‑‑ttt                                                               >  1:1298752/1‑71 (MQ=255)   ccaAACAGAATCCGTTCCTCACCGTTTAATAACAGCTGGATATCAATGTATTGCTCGTGCAATTCCGC‑‑ttt                                                               >  1:1290152/1‑71 (MQ=255)   ccaAACAGAATCCGTTCCTCACCGTTTAATAACAGCTGGATATCAATGTATTGCTCGTGCAATTCCGC‑‑ttt                                                               >  1:4614059/1‑71 (MQ=255)   ccaAACAGAATCCGTTCCTCACCGTTTAATAACAGCTGGATATCAATGTATTGCTCGTGCAATTCCGC‑‑ttt                                                               >  1:436565/1‑71 (MQ=255)   ccaAACAGAATCCGTTCCTCACCGTTTAATAACAGCTGGATATCAATGTATTGCTCGTGCAATTCCGC‑‑ttt                                                               >  1:3771401/1‑71 (MQ=255)   acaAACAGAATCCGTTCCTCACCGTTTAATAACAGCTGGATATCAATGTATTGCTCGTGCAATTCCGC‑‑ttt                                                               >  1:2358398/2‑71 (MQ=255)            aTCCGTTCCTCACCGTTTAATAACAGCTGGATATCAATGTATTGCTCGTGCAATTCCGCT‑TTTTTTCTCGa                                                       >  1:2567935/1‑71 (MQ=255)             tCCGTTCCTCACCGTTTAATAACAGCTGGATATCAATGTATTGCTCGTGCAATTCCGCT‑TTTTTTCTCGAc                                                      >  1:4083237/1‑71 (MQ=255)               cGTTCCTCACCGTTTAATAACAGCTGGATATCAATGTATTGCTCGTGCAATTCCGCT‑TTTTTTCTCGACgg                                                    >  1:4493376/1‑71 (MQ=255)               cGTTCCTCACCGTTTAATAACAGCTGGATATCAATGTATTGCTCGTGCAATTCCGCT‑TTTTTTCTCGACgg                                                    >  1:3853691/1‑71 (MQ=255)                 ttCCTCAGCGTTTAATAACAGCTGGATATCAATGTATTGCGCGTGCAATTCCGCT‑TTTTTTCTCGACGGGc                                                  <  1:2873251/71‑1 (MQ=255)                 ttCCTCACCGTTTAATAACAGCTGGATATCAATGTATTGCTCGTGCAATTCCGCT‑TTTTTTCTCGACGGGc                                                  <  1:3589587/71‑1 (MQ=255)                 ttCCTCACCGTTTAATAACAGCTGGATATCAATGTATTGCTCGTGCAATTCCGCT‑TTTTTTCTCGACGGGc                                                  >  1:1546597/1‑71 (MQ=255)                  tCCTCACCGTTTAATAACAGCTGGATATCAATGTATTGCTCGTGCAATTCCGCT‑TTTTTTCTCGACGGGc                                                  >  1:3941704/1‑70 (MQ=255)                   ccTCACCGTTTAATAACAGCTGGATATCAATGTATTGCTCGTGCAATTCCGCT‑TTTTTTCTCGACGGGCg                                                 <  1:862079/70‑1 (MQ=255)                   ccTCACCGTTTAATAACAGCTGGATATCAATGTATTGCTCGTGCAATTCCGCT‑TTTTTTCTCGACGGGCGa                                                >  1:1737138/1‑71 (MQ=255)                   ccTCACCGTTTAATAACAGCTGGATATCAATGTATTGCTCGTGCAATTCCGCT‑TTTTTTCTCGACGGGCGa                                                >  1:3746706/1‑71 (MQ=255)                   ccTCACCGTTTAATAACAGCTGGATATCAATGTATTGCTCGTGCAATTCCGCT‑TTTTTTCTCGACGGGCGa                                                >  1:1129836/1‑71 (MQ=255)                   ccTCACCGTTTAATAACAGCTGGATATCAATGTATTGCTCGTGCAATTCCGCT‑TTTTTTCTCGACGGGCGa                                                >  1:3997985/1‑71 (MQ=255)                   ccTCACCGTTTAATAACAGCTGGATATCAATGTATTGCTCGTGCAATTCCGCT‑TTTTTTCTCGACGGGCGa                                                >  1:4032707/1‑71 (MQ=255)                   ccTCACCGTTTAATAACAGCTGGATATCAATGTATTGCTCGTGCAATTCCGCT‑TTTTTTCTCGACGGGCGa                                                >  1:1691781/1‑71 (MQ=255)                    cTCACCGTTTAATAACAGCTGGATATCAATGTATTGCTCGTGCAATTCCGCT‑TTTTTTCTCGACGGGCGa                                                <  1:2590962/70‑1 (MQ=255)                       aCCGTTTAATAACAGCTGGATATCAATGTATTGCTCGTGCAATTCCGCT‑TTTTTTCTCGACGGGCGATTg                                             <  1:3149470/70‑1 (MQ=255)                       aCCGTTTAATAACAGCTGGATATCAATGCATTGCTCGTGCAATTCCGCT‑TTTTTTCTCGACGGGCGATTg                                             <  1:3182090/70‑1 (MQ=255)                        ccGTTTAATAACAGCTGGATATCAATGTATTGCTCGTGCAATTCCGCT‑TTTTTTCTCGACGGGCGATTGAg                                           <  1:1436631/71‑1 (MQ=255)                        ccGTTTAATAACAGCTGGATATCAATGTATTGCTCGTGCAATTCCGCT‑TTTTTTCTCGACGGGCGATTGAg                                           <  1:749920/71‑1 (MQ=255)                        ccGTTTAATAACAGCTGGATATCAATGTATTGCTCGTGCAATTCCGCT‑TTTTTTCTCGACGGGCGATTGAg                                           <  1:825729/71‑1 (MQ=255)                         cGTTTAATAACAGCTGGATATCAATGTATTGCTCGTGCAATTCCGCT‑TTTTTTCTCGACGGGCGATTGAgt                                          >  1:4083070/1‑71 (MQ=255)                             taataaCAGCTGGATATCAATGTATTGCTCGTGCAATTCCGCT‑TTTTTTCTCGACGGGCGATTGAGTGtt                                       >  1:816434/1‑70 (MQ=255)                              aataaCAGCTGGATATCAATGTATTGCTCGTTCAATTCCGCT‑TTTTTTCTCGACGGGCGATTGAGTGTTa                                      <  1:4034814/70‑1 (MQ=255)                              aataaCAGCTGGATATCAATGTATTGCTCGTGCAATTCCGCT‑TTTTTTCTCGACGGGCGATTGAGTGTTa                                      <  1:3464552/70‑1 (MQ=255)                                taaCAGCTGGATATCAATGTATTGCTCGTGCAATTCCGCT‑TTTTTTCTCGACGGGCGATTGAg                                           <  1:2337423/63‑1 (MQ=255)                                taaCAGCTGGATATCAATGTATTGCTCGTGCAATTCCGCT‑TTTTTTCTCGACGGGCGATTGAGTGTTa                                      <  1:1186999/68‑1 (MQ=255)                                  aCAGCTGGATATCAATGTATTGCTCGTGCAATTCCGCT‑TTTTTTCTCGACg                                                     >  1:52810/1‑51 (MQ=255)                                  aCAGCTGGATATCAATGTATTGCTCGTGCAATTCCGCT‑TTTTTTCTCGACGGGCGATTGAgtg                                         >  1:4266607/1‑63 (MQ=255)                                  aCAGCTGGATATCAATGTATTGCTCGTGCAATTCCGCT‑TTTTTTCTCGACGGGCGATTGAg                                           >  1:109477/1‑61 (MQ=255)                                  aCAGCTGGATATCAATGTATTGCTCGTGCAATTCCGCT‑TTTTTTCTCGACGGGCGATTGAGTGTTaaa                                    >  1:4297920/1‑68 (MQ=255)                                  aCAGCTGGATATCAATGTATTGCTCGTGCAATTCCGCT‑TTTTTTCTCGACGGGCGATTGAGTGTTAAACGt                                 <  1:3014550/71‑1 (MQ=255)                                  aCAGCTGGATATCAATGTATTGCTCGTGCAATTCCGCT‑TTTTTTCTCGACGGGCGATTGAGTGTTAAACGt                                 <  1:4523140/71‑1 (MQ=255)                                  aCAGCTGGATATCAATGTATTGCTCGTGCAATTCCGCT‑TTTTTTCTCGACGGGCGATTGAGTGTTAAACGt                                 <  1:4351833/71‑1 (MQ=255)                                   cAGCTGGATATCAATGTATTGCTCGTGCAATTCCGCT‑TTTTTTCTCGACGGGCGATTGAGTGTTAAACGTc                                <  1:1233219/71‑1 (MQ=255)                                         gATATCAATGTATTGCTCGTGCAATTCCGCT‑TTTTTTCTCGACGGGCGATTGAGTGTTAAACGTCATGa                            <  1:404267/69‑1 (MQ=255)                                         gATATCAATGTATTGCTCGTGCAATTCCGCT‑TTTTTTCTCGACGGGCGATTGAGTGTTAAACGTCATGACa                          <  1:4624933/71‑1 (MQ=255)                                            atCAATGTATTGCTCGTGCAATTCCGCT‑TTTTTTCTCGACGGGCGATTGAGTGTTAAACGTCATGACAtt                        >  1:3324642/1‑70 (MQ=255)                                            atCAATGTATTGCTCGTGCAATTCCGCT‑TTTTTTCTCGACGGGCGATTGAGTGTTAAACGTCATGACAtt                        >  1:538035/1‑70 (MQ=255)                                             tCAATGTATTGCTCGTGCAATTCCGCT‑TTTTTTCTCGACGGGCGATTGAGTGTTAAACGTCATGACATTCa                      >  1:138544/1‑71 (MQ=255)                                              cAATGTATTGCTCGTGCAATTCCGCT‑TTTTTTCTCGAgggg                                                   >  1:203548/1‑41 (MQ=37)                                               aaTGTATTGCTCGTGCAATTCCGCT‑TTTTTTCTCGACGGGCGATTGAGTGTTAAACGTCATGACATTCAt                     >  1:1071028/1‑70 (MQ=255)                                                   tATTGCTCGTGCAATTCCGCT‑TTTTTTCTCGACGGGCGATTGAGTGTTAAACGTCATGACATTCATa                    >  1:1224716/1‑67 (MQ=255)                                                   tATTGCTCGTGCAATTCCGCT‑TTTTTTCTCGACGGGCGATTGAGTGTTAAACGTCATGACATTCATa                    >  1:3555194/1‑67 (MQ=255)                                                   tATTGCTCGTGCAATTCCGCT‑TTTTTTCTCGACGGGCGATTGAGTGTTAAACGTCATGACATTCATa                    >  1:211603/1‑67 (MQ=255)                                                   tATTGCTCGTGCAATTCCGCT‑TTTTTTCTCGACGGGCGATTGAGTGTTAAACGTCATGACATTCATa                    >  1:1743008/1‑67 (MQ=255)                                                   tATTGCTCGTGCAATTCCGCT‑TTTTTTCTCGACGGGCGATTGAGTGTTAAACGTCATGACATTCATAAAGa                <  1:4640560/71‑1 (MQ=255)                                                   tATTGCTCGTGCAATTCCGCT‑TTTTTTCTCGACGGGCGATTGAGTGTTAAACGTCATGACATTCATAAAGa                <  1:380065/71‑1 (MQ=255)                                                   tATTGCTCGTGCAATTCCGCT‑TTTTTTCTCGACGGGCGATTGAGTGTTAAACGTCATGACATTCATAAAGa                <  1:2454523/71‑1 (MQ=255)                                                   tATTGCTCGTGCAATTCCGCT‑TTTTTTCTCGACGGGCGATTGAGTGTTAAACGTCATGACATTCATAAAGa                <  1:1525522/71‑1 (MQ=255)                                                   tATTGCTCGTGCAATTCCGCT‑TTTTTTCTCGACGGGCGATTGAGTGTTAAACGTCATGACATTCATAAAGa                <  1:3392909/71‑1 (MQ=255)                                                     ttGCTCGTGCAATTCCGCTTTTTTTTCTCGACGGGCGATTGAGTGTTAAACGTCATGACATTCATAAAGat               <  1:3567751/71‑1 (MQ=255)                                                     ttGCTCGTGCAATTCCGCT‑TTTTTTCTCGACGGGCGATTGAGTGTTAAACGTCATGACATTCATAAAGata              >  1:633303/1‑71 (MQ=255)                                                     ttGCTCGTGCAATTCCGCT‑TTTTTTCTCGACGGGCGATTGAGTGTTAAACGTCATGACATTCATAAAGata              >  1:171498/1‑71 (MQ=255)                                                     ttGCTCGTGCAATTCCGCT‑TTTTTTCTCGACGGGCGATTGAGTGTTAAACGTCATGACATTCATAAAGat               <  1:983562/70‑1 (MQ=255)                                                     ttGCTCGTGCAATTCCGCT‑TTTTTTCTCGACGGGCGATTGAGTGTTAAACGTCATGACATTCATAAAGat               <  1:4686766/70‑1 (MQ=255)                                                     ttGCTCGTGCAATTCCGCT‑TTTTTTCTCGACGGGCGATTGAGTGTTAAACGTCATGACATTCATAAAGat               <  1:3939325/70‑1 (MQ=255)                                                     ttGCTCGTGCAATTCCGCT‑TTTTTTCTCGACGGGCGATTGAGTGTTAAACGTCATGACATTCATAAAGat               <  1:3167885/70‑1 (MQ=255)                                                     ttGCTCGTGCAATTCCGCT‑TTTTTTCTCGACGGGCGATTGAGTGTTAAACGTCATGACATTCATAAAGat               <  1:3022004/70‑1 (MQ=255)                                                     ttGCTCGTGCAATTCCGCT‑TTTTTTCTCGACGGGCGATTGAGTGTTAAACGTCATGACATTCATAAAGat               <  1:2933947/70‑1 (MQ=255)                                                     ttGCTCGTGCAATTCCGCT‑TTTTTTCTCGACGGGCGATTGAGTGTTAAACGTCATGACATTCATAAAGat               <  1:2919940/70‑1 (MQ=255)                                                      tGCTCGTGCAATTCCGCT‑TTTTTTCTCGACGGGCGATTGAGTGTTAAACGTCATGACa                          >  1:3526957/1‑58 (MQ=255)                                                      tGCTCGTGCAATTCCGCT‑TTTTTTCTCGACGGGCGATTGAGTGTTAAACGTCATGACa                          >  1:2573100/1‑58 (MQ=255)                                                      tGCTCGTGCAATTCCGCT‑TTTTTTCTCGACGGGCGATTGAGTGTTAAACGTCATGACATTCATAAAGat               <  1:3253675/69‑1 (MQ=255)                                                          cGTGCAATTCCGCT‑TTTTTTCTCGACGGGCGATTGAGTGTTAAACGTCATGACATTCATAAAGATATTGTc         >  1:2416279/1‑71 (MQ=255)                                                            tGCAATTCCGCT‑TTTTTTCTCGACGGGCGATTGAGTGTTAAACGTCATGACATTCATAAAGATAtt            <  1:1395371/66‑1 (MQ=255)                                                            tGCAATTCCGCT‑TTTTTTCTCGACGGGCGATTGAGTGTTAAACGTCATGACATTCATAAAGATATTGTCGc       <  1:1380458/71‑1 (MQ=255)                                                              cAATTCCGCT‑TTTTTTCTCGACGGGCGATTGAGTGTTAAACGTCATGACATTCATAAAGa                >  1:838725/1‑60 (MQ=255)                                                              cAATTCCGCT‑TTTTTTCTCGACGGGCGATTGAGTGTTAAACGTCATGACATTCATAAAGATATTGTCGccc     >  1:4016232/1‑71 (MQ=255)                                                              cAATTCCGCT‑TTTTTTCTCGACGGGCGATTGAGTGTTAAACGTCATGACATTCATAAAGATATTGTCGccc     <  1:1881966/71‑1 (MQ=255)                                                                 ttCCGCT‑TTTTTTCTCGACGGGCGATTGAGTGTTAAACGTCATGACATTCATAAAGat               <  1:4216305/58‑1 (MQ=255)                                                                 ttCCGCT‑TTTTTTCTCGACGGGCGATTGAGTGTTAAACGTCATGACATTCATAAAGATATTGTCGCCCTGt  <  1:608835/71‑1 (MQ=255)                                                                 ttCCGCT‑TTTTTTCTCGACGGGCGATTGAGTGTTAAACGTCATGACATTCATAAAGATATTGTCGCCCTGt  <  1:3698717/71‑1 (MQ=255)                                                                 ttCCGCT‑TTTTTTCTCGACGGGCGATTGAGTGTTAAACGTCATGACATTCATAAAGATATTGTCGCCCTGt  <  1:1029134/71‑1 (MQ=255)                                                                  tCCGCT‑TTTTTTCTCGACGGGCGATTGAGTGTTAAACGTCATGACATTCATAAAGATATTGTCGCCCTGt  >  1:3541317/1‑70 (MQ=255)                                                                  tCCGCT‑TTTTTTCTCGACGGGCGATTGAGTGTTAAACGTCATGACATTCATAAAGATATTGTCGCCCTGt  >  1:3766019/1‑70 (MQ=255)                                                                       |                                                                  TGCCAAACAGAATCCGTTCCTCACCGTTTAATAACAGCTGGATATCAATGTATTGCTCGTGCAATTCCGC‑‑TTTTTTCTCGACGGGCGATTGAGTGTTAAACGTCATGACATTCATAAAGATATTGTCGCCCTGT  >  W3110S.gb/3369081‑3369214

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑