| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 3,493,301 | +A | coding (851/1080 nt) | frwC ← | predicted enzyme IIC component of PTS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,493,298 | 1 | . | A | 98.9% | 275.2 / ‑3.4 | 92 | coding (854/1080 nt) | frwC | predicted enzyme IIC component of PTS |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base A (49/42); total (49/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01 | |||||||||||
Only 100 of 103 total aligned reads displayed.
GGCAGCACAATCAGACCACCCCAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCAC‑AAA‑CTGAACCGACCATGATGGAAGGAATGACACGCAGCGGATCGGCAGCGGCGAAAGGAATCGCC > W3110S.gb/3493229‑3493363 | ggCAGCACAATCAGACCACCCCAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGcaccac > 1:2908520/1‑70 (MQ=255) acaATCAGACCACCCCAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCAC‑aaaact > 1:1356472/1‑67 (MQ=255) acaATCAGACCACCCCAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCAC‑aaaact > 1:4494431/1‑67 (MQ=255) acaATCAGACCACCCCAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCAC‑aaaact > 1:1569096/1‑67 (MQ=255) acaATCAGACCACCCCAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCAC‑aaaact > 1:3673124/1‑67 (MQ=255) tCAGACCACCCCAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGAAcc < 1:2186587/71‑1 (MQ=255) tCAGACCACCCCAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGAAcc > 1:2930281/1‑71 (MQ=255) tCAGACCACCCCAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGAAcc < 1:1848577/71‑1 (MQ=255) tCAGACCACCCCAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGAAc > 1:3144808/1‑70 (MQ=255) cAGACAACCCCAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGAAcc > 1:2974530/1‑70 (MQ=255) gACCACCCCAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGAACCGAc < 1:1135207/71‑1 (MQ=255) gACCACCCCAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGAACCGAc < 1:4581590/71‑1 (MQ=255) ccaccCCAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGAACCGACCa > 1:1081914/1‑71 (MQ=255) caccCCAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGAACCTACCat < 1:1367852/71‑1 (MQ=255) caccCCAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGAACCGACTat < 1:413529/71‑1 (MQ=255) caccCCAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGAACCGACCat < 1:3172858/71‑1 (MQ=255) caccCCAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGAACCGACCat < 1:854461/71‑1 (MQ=255) caccCCAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGAACCGACCat < 1:2137931/71‑1 (MQ=255) caccCCAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCAC‑AAACCTGAACCGACCat < 1:3337242/71‑1 (MQ=255) ccAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGAACCGACCatgatg > 1:530754/1‑71 (MQ=255) ccAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGAACCGACCatgatg > 1:1334673/1‑71 (MQ=255) ccAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGAACCGACCatgatg > 1:943172/1‑71 (MQ=255) cTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGAACCTACCATGATggaagg < 1:2187113/71‑1 (MQ=255) cTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGAACCGACCATGATggaagg < 1:3728017/71‑1 (MQ=255) cTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGAACCGACCATGATggaagg < 1:1089566/71‑1 (MQ=255) cTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGAACCGACCATGATggaagg < 1:4431773/71‑1 (MQ=255) cTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGAACCGACCATGATggaagg < 1:2982784/71‑1 (MQ=255) cTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGAACCGACCATGATggaagg < 1:754701/71‑1 (MQ=255) gCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGAACCGACCATGATggaaggaa > 1:2315479/1‑71 (MQ=255) gCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGAACCGACCATGATggaaggaa > 1:2580879/1‑71 (MQ=255) cATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGAACCGACCATGATggaagga < 1:1535159/69‑1 (MQ=255) cATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGAACCGACCATGATggaagg < 1:2846081/68‑1 (MQ=255) cATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGAACCGACCATGATGGAAGGAAt < 1:654904/71‑1 (MQ=255) cATAGCACTGCGCACCGACCAGCGCCGAAGTTACTGCACCACAAAA‑CTGAACCGACCATGATGGAAGGAAt < 1:2672746/71‑1 (MQ=255) tAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGAACCGACCATGATGGAAGGAATGa < 1:3617619/71‑1 (MQ=255) tAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGAACCGACCATGATGGAAGGAATGa < 1:1835821/71‑1 (MQ=255) tAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGAACCGACCATGATGGAAGGAATGa < 1:3265386/71‑1 (MQ=255) tAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGAACCGACCATGATGGAAGGAATGa < 1:2807371/71‑1 (MQ=255) tAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGAACCGACCATGATGGAAGGAATGa < 1:808666/71‑1 (MQ=255) tAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGAACCGACCATGATGGAAGGAATGa > 1:2326174/1‑71 (MQ=255) gCACTGCGCACCGCCCAGCGCCGCAGTTACTGCACCACAAAA‑CTGAACCGACCATGATGGAAGGAATGaca > 1:3776659/1‑71 (MQ=255) gCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGaa > 1:2690187/1‑47 (MQ=38) gCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGaa > 1:4584204/1‑47 (MQ=38) gCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGAACCGa > 1:2276868/1‑51 (MQ=38) gCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGAACCGACCatg > 1:1959357/1‑56 (MQ=39) gCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGAACCGACCATGATggaaggaa > 1:1689045/1‑66 (MQ=255) gCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGAACCGACCATGATGGAAGGAAt > 1:2803396/1‑67 (MQ=255) gCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGAACCGACCATGATGGAAGGAATGaca > 1:2788639/1‑71 (MQ=255) gCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGAACCGACCATGATGGAAGGAATGaca > 1:2434097/1‑71 (MQ=255) gCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGAACCGACCATGATGGAAGGAATGaca > 1:303765/1‑71 (MQ=255) cTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGAACCGACCATGATGGAAGGAATGACACGc < 1:3789032/71‑1 (MQ=255) tGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGAACCGACCATGATggaagg < 1:3898809/60‑1 (MQ=255) tGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGAACCGACCATGATggaagg < 1:1940941/60‑1 (MQ=255) gcgcACCGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGAACCGACCATGATGGAAGGAATGACACGCAg > 1:1275068/1‑71 (MQ=255) gcgcACCGACCAGCGCCGCAGTTACTGCACCAC‑aaaa > 1:4445190/1‑36 (MQ=255) cgcACGGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGAACCGACCATGATGGAAGGAATGACACGCAGc > 1:2493045/1‑71 (MQ=255) cgcACCGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGAACCGACCATGATGGAAGGAATGACACGCAGc > 1:566173/1‑71 (MQ=255) gcACCGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGAACCGACCATGATggaagg < 1:144932/57‑1 (MQ=39) cGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGAACCGACCATGATGGAAGGAATGACACGCAGCGGATc < 1:4206556/71‑1 (MQ=255) cGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGAACCGACCATGATGGAAGGAATGACACGCAGCGGATc < 1:753602/71‑1 (MQ=255) cGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGAACCGACCATGATGGAAGGAATGACACGCAGCGGATc < 1:1181284/71‑1 (MQ=255) gcgcCGCAGTTACTGCACCACAAAA‑CTGAACCGACCATGATGGAAGGAATGACACGCAGCGGATCGGCAgc < 1:1895033/71‑1 (MQ=255) cgccgcAGTTACTGCACCACAAAA‑CTGAACCGACCATGATGGAAGGAATGACACGCAGCGGATCGGCAgcg > 1:3426649/1‑71 (MQ=255) cgccgcAGTTACTGCACCACAAAA‑CTGAACCGACCATGATGGAAGGAATGACACGCAGCGGATCGGCAgcg > 1:2097316/1‑71 (MQ=255) cgccgcAGTTACTGCACCACAAAA‑CTGAACCGACCATGATGGAAGGAATGACACGCAGCGGATCGGCAgcg > 1:3165307/1‑71 (MQ=255) gccgcAGTTACTGCACCACAAAA‑CTGAACCGACCATGATggaaggaa > 1:1454200/1‑47 (MQ=39) gccgcAGTTACTGCACCACAAAA‑CTGAACCGACCATGATGGAAGGAATGACACGCAGCGGATCGGCAgcgg > 1:490150/1‑71 (MQ=255) cAGTTACTGCACCACAAAA‑CTGAACCGACCATGATggaagga < 1:1367407/42‑1 (MQ=255) cAGTTACTGCACCACAAAA‑CTGAACCGACCATGATggaagga < 1:3712414/42‑1 (MQ=255) cAGTTACTGCACCACAAAA‑CTGAACCGACCATGATggaagga < 1:3150647/42‑1 (MQ=255) ttACTGCACCACAAAA‑CTGAACCGACCATGATggaagg < 1:2321156/38‑1 (MQ=38) ttACTGCACCACAAAA‑CTGAACCGACCATGATggaagg < 1:4010313/38‑1 (MQ=38) tACTGCACCACAAAA‑CTGAACCGACCATGATGGAAGGAATGACACGCAGCGGATCGGCAGCGGCGAAAgg > 1:2215352/1‑70 (MQ=255) tACTGCACCACAAAA‑CTGAACCGACCATGATGGAAGGAATGACACGCAGCGGATCGGCAGCGGCGAAAgg > 1:2520118/1‑70 (MQ=255) tACTGCACCACAAAA‑CTGAACCGACCATGATGGAAGGAATGACACGCAGCGGATCGGCAGCGGCGAAAgg > 1:713655/1‑70 (MQ=255) tACTGCACCACAAAA‑CTGAACCGACCATGATGGAAGGAATGACACGCAGCGGATCGGCAGCGGCGAAAgg > 1:4101341/1‑70 (MQ=255) aCTGCACCACAAAA‑CTGAACCGACCATGATGGAAGGAATGACACGCAGCGGATCGGCAGCGGCGaaa > 1:3883251/1‑67 (MQ=255) aCTGCACCACAAAA‑CTGAACCGACCATGATGGAAGGAATGACACGCAGCGGATCGGCAGCGGCGAAAg > 1:3107740/1‑68 (MQ=255) aCTGCACCACAAAA‑CTGAACCGACCATGATGGAAGGAATGACACGCAGCGGATCGGCAGCGGCGAAAGGaa > 1:642337/1‑71 (MQ=255) aCTGCACCACAAAA‑CTGAACCGACCATGATGGAAGGAATGACACGCAGCGGATCGGCAGCGGCGAAAGGaa > 1:3491298/1‑71 (MQ=255) aCTGCACCACAAAA‑CTGAACCGACCATGATGGAAGGAATGACACGCAGCGGATCGGCAGCGGCGAAAGGaa > 1:2603950/1‑71 (MQ=255) aCTGCACCACAAAA‑CTGAACCGACCATGATGGAAGGAATGACACGCAGCGGATCGGCAGCGGCGAAAGGaa > 1:2385138/1‑71 (MQ=255) aCTGCACCACAAAA‑CTGAACCGACCATGATGGAAGGAATGACACGCAGCGGATCGGCAGCGGCGAAAGGaa > 1:3100736/1‑71 (MQ=255) aCTGCACCACAAAA‑CTGAACCGACCATGATGGAAGGAATGACACGCAGCGGATCGGCAGCGGCGAAAGGaa > 1:3425972/1‑71 (MQ=255) aCTGCACCACAAAA‑CTGAACCGACCATGATGGAAGGAATGACACGCAGCGGATCGGCAGCGGCGAAAGGaa > 1:3135558/1‑71 (MQ=255) aCTGCACCACAAAA‑CTGAACCGACCATGATGGAAGGAATGACACGCAGCGGATCGGCAGCGGCGAAAGGaa > 1:3407848/1‑71 (MQ=255) aCTGCACCACAAAA‑CTGAACCGACCATGATGGAAGGAATGACACGCAGCGGATCGGCAGCGGCGAAAGGa > 1:4350867/1‑70 (MQ=255) aCTGCACCACAAAA‑CTGAACCGACCATGATGGAAGGAATGACACGCAGCGGATCGGCAGCGGCGAAAGGa < 1:3551975/70‑1 (MQ=255) aCTGCACCACAAAA‑CTGAACCGACCATGATGGAAGGAATGACACGCAGCGGATCGGCAGCGGCGAAAGGa < 1:883358/70‑1 (MQ=255) aCTGCACCACAAAA‑CTGAACCGACCATGATGGAAGGAATGACACGCAGCGGATCGGCAGCGGCGAAAGGa < 1:1701069/70‑1 (MQ=255) aCTGCACCACAAAA‑CTGAACCGACCATGATGGAAGGAATGACACGCAGCGGATCGGCAGCGGCGAAAGGa < 1:415576/70‑1 (MQ=255) aCTGCACCACAAAA‑CTGAACCGACCATGATGGAAGGAATGACACGCAGCGGATCGGCAGCGGCGAAAGGa > 1:2060833/1‑70 (MQ=255) aCTGCACCACAAAA‑CTGAACCGACCATGATGGAAGGAATGACACGCAGCGGATCGGCAGCGGCGAAAGGa > 1:1969076/1‑70 (MQ=255) aCTGCACCACAAAA‑CTGAACCGACCATGATGGAAGGAATGACACGCAGCGGATCGGCAGCGGCGAAAGGa > 1:4545293/1‑70 (MQ=255) aCTGCACCACAAAA‑CTGAACCGACCATGATGGAAGGAATGACACGCAGCGGATCGGCAGCGGCGAAAGGa < 1:4439089/70‑1 (MQ=255) caccacaAAA‑CTGAACCGACCATGATGGAAGGAATGACACGCAGCGGATCGGCAGCGGCGAAAGGAATCGc < 1:4118535/71‑1 (MQ=255) caccacaAAA‑CTGAACCGACCATGATGGAAGGAATGACACGCAGCGGATCGGCAGCGGCGAAAGGAATCGc < 1:3584047/71‑1 (MQ=255) accacaAAA‑CTGAACCGACCATTATGGAAGGAATGACACGCAGCGGATCGGCAGCGGCGAAAGGAATCGcc < 1:1392130/71‑1 (MQ=255) accacaAAA‑CTGAACCGACCATGATGGAAGGAATGACACGCAGCGGATCGGCAGCGGCGAAAGGAATCGcc < 1:3864295/71‑1 (MQ=255) accacaAAA‑CTGAACCGACCATGATGGAAGGAATGACACGCAGCGGATCGGCAGCGGCGAAAGGAATCGcc < 1:1807212/71‑1 (MQ=255) | GGCAGCACAATCAGACCACCCCAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCAC‑AAA‑CTGAACCGACCATGATGGAAGGAATGACACGCAGCGGATCGGCAGCGGCGAAAGGAATCGCC > W3110S.gb/3493229‑3493363 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |