| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 1,846,641 | (A)8→7 | intergenic (‑56/+72) | ynjI ← / ← topB | predicted inner membrane protein/DNA topoisomerase III |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,846,634 | 0 | A | . | 91.8% | 225.6 / 16.0 | 61 | intergenic (‑49/+79) | ynjI/topB | predicted inner membrane protein/DNA topoisomerase III |
| Reads supporting (aligned to +/- strand): ref base A (5/0); new base . (20/36); total (25/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.93e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.29e-01 | |||||||||||
ACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGCAAAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGAATATGACTCGAATAG > W3110S.gb/1846587‑1846694 | acaTTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTc < 1:3590215/70‑1 (MQ=255)acaTTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTc < 1:1035379/70‑1 (MQ=255)acaTTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTc < 1:3211373/70‑1 (MQ=255)acaTTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTc < 1:17725/70‑1 (MQ=255)acaTTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTc < 1:1880914/70‑1 (MQ=255)acaTTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTc < 1:1386592/70‑1 (MQ=255)acaTTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTc < 1:4056874/70‑1 (MQ=255) caTTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAAtt < 1:306341/66‑1 (MQ=255) caTTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAAtt < 1:1876109/66‑1 (MQ=255) caTTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAAtt < 1:273943/66‑1 (MQ=255) caTTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAAtt < 1:3226897/66‑1 (MQ=255) ttCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAAtttt > 1:45249/1‑66 (MQ=255) ttCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAAtttt > 1:1649387/1‑66 (MQ=255) cccTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATc > 1:1444205/1‑71 (MQ=255) cccTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATc > 1:3414035/1‑71 (MQ=255) cccTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATc > 1:2498872/1‑71 (MQ=255) cccTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATc > 1:690506/1‑71 (MQ=255) cccTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATc > 1:3662852/1‑71 (MQ=255) cccTGTTATTACATACTTTAGAGGCGATAATTTTATCAATGC‑AAAAAAATATCCAACGAATTTTCTTGATc > 1:1058048/1‑71 (MQ=255) attaCATACTTTAGAGGCGATAATTTTATCCATGCAAAAAAAATATCCAACGAATTTTCTTGATCCGCTg > 1:1815997/1‑70 (MQ=255) attaCATACTTTAGAGGCGATAATTTTATCCATGCAAAAAAAATATCCAACGAATTTTCTTGATCCGCTg > 1:3853145/1‑70 (MQ=255) attaCATACTTTAGAGGCGATAATTTTATCCATGCAAAAAAAATATCCAACGAATTTTCTTGATCCGCTg > 1:1910043/1‑70 (MQ=255) attaCATACTTTAGAGGCGATAATTTTATCCATGCAAAAAAAATATCCAACGAATTTTCTTGATCCGCTg > 1:308919/1‑70 (MQ=255) attaCATACTTTAGAGGCGATAATTTTATCCATGCAAAAAAAATATCCAACGAATTTTCTTGATCCGCTg > 1:781747/1‑70 (MQ=255) aCATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGatat > 1:3047274/1‑71 (MQ=255) tAGAGGCGATAATTTTCTCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGaa < 1:55857/70‑1 (MQ=255) tAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGaa < 1:377783/70‑1 (MQ=255) tAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGaa < 1:3897391/70‑1 (MQ=255) tAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGaa < 1:2281505/70‑1 (MQ=255) tAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGaa < 1:3457740/70‑1 (MQ=255) tAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGaa < 1:3535844/70‑1 (MQ=255) gagGCTATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGc < 1:2856854/54‑1 (MQ=255) gagGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGc < 1:3921245/54‑1 (MQ=255) gagGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGc < 1:500849/54‑1 (MQ=255) gagGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGc < 1:2221321/54‑1 (MQ=255) gagGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGaa < 1:992796/68‑1 (MQ=255) gagGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGaa < 1:3089790/68‑1 (MQ=255) gagGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGaa < 1:140205/68‑1 (MQ=255) gagGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGaa < 1:1828827/68‑1 (MQ=255) gagGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGaa < 1:1417644/68‑1 (MQ=255) gagGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGaa < 1:1310178/68‑1 (MQ=255) cGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGaa < 1:1237583/64‑1 (MQ=255) aTAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGAATATGACTCg < 1:2726719/71‑1 (MQ=255) aTAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGAATATGACTCg < 1:4119888/71‑1 (MQ=255) aTAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGAATATGACTCg < 1:759893/71‑1 (MQ=255) aTAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGAATATGACTCg < 1:1102902/71‑1 (MQ=255) aTAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGAATATGACTCg < 1:3925603/71‑1 (MQ=255) tAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGAATATGACTCg < 1:2756054/70‑1 (MQ=255) tAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGAATATGACTCg < 1:19840/70‑1 (MQ=255) tAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGAATATGACTCg < 1:4050674/70‑1 (MQ=255) aaTTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATAtt > 1:3783812/1‑54 (MQ=255) aaTTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATAtt > 1:1716630/1‑54 (MQ=255) aaTTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGAATATGACTCGaa > 1:1650414/1‑71 (MQ=255) aaTTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGAATATGACTCGaa > 1:1090391/1‑71 (MQ=255) aaTTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGAATATGACTCGaa > 1:1085698/1‑71 (MQ=255) tttATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGAATATGACTCGAATAg > 1:3069518/1‑71 (MQ=255) tttATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGAATATGACTCGAATAg > 1:1681554/1‑71 (MQ=255) tttATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGAATATGACTCGAATAg > 1:1775592/1‑71 (MQ=255) tttATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGAATATGACTCGAATAg > 1:1844518/1‑71 (MQ=255) tttATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGAATATGACTCGAATAg > 1:2086411/1‑71 (MQ=255) tttATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGAATATGACTCGAATAg > 1:2914518/1‑71 (MQ=255) | ACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGCAAAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGAATATGACTCGAATAG > W3110S.gb/1846587‑1846694 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |