| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 547,835 | +G | pseudogene (255/261 nt) pseudogene (255/1259 nt) |
ylbE → ylbE → |
ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment ECK0512:JW0508+JW0507:b4572; hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 547,831 | 1 | . | G | 97.2% | 124.5 / ‑3.6 | 36 | pseudogene (251/261 nt) pseudogene (251/1259 nt) | ylbE ylbE | ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment ECK0512:JW0508+JW0507:b4572; hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (10/25); total (10/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01 | |||||||||||
GCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTAT > W3110S.gb/547771‑547890 | gcgcATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑GGGTGAAGTg < 1:3058/71‑1 (MQ=255) cATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAAc < 1:1388174/71‑1 (MQ=255) cATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAAc < 1:481687/71‑1 (MQ=255) cATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAAc < 1:3449605/71‑1 (MQ=255) cATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAAc < 1:2596054/71‑1 (MQ=255) cATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAAc < 1:674442/71‑1 (MQ=255) tGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCAtt > 1:502765/1‑71 (MQ=255) tGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCAtt > 1:19023/1‑71 (MQ=255) tGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCAtt > 1:2771568/1‑71 (MQ=255) tGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCAt > 1:1705612/1‑70 (MQ=255) ggTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑g > 1:2305208/1‑45 (MQ=255) ttGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCAcc < 1:3865089/71‑1 (MQ=255) ttGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCAcc < 1:713975/71‑1 (MQ=255) ttGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCAcc < 1:3471555/71‑1 (MQ=255) ggCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCAt < 1:679854/70‑1 (MQ=255) ggCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCAt < 1:3713231/70‑1 (MQ=255) ggCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCAt < 1:2850138/70‑1 (MQ=255) ggCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCAt < 1:2998943/70‑1 (MQ=255) ggCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCAt < 1:3155072/70‑1 (MQ=255) gCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCAt < 1:1567788/69‑1 (MQ=255) tGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACt > 1:2169057/1‑44 (MQ=38) tGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACt > 1:3060881/1‑44 (MQ=38) tGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACt > 1:2318940/1‑44 (MQ=38) tGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACt > 1:2072930/1‑44 (MQ=38) gAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGt > 1:625732/1‑71 (MQ=255) gAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGt > 1:250001/1‑71 (MQ=255) cgcAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCggg < 1:3650482/71‑1 (MQ=255) gcAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCggg < 1:730496/70‑1 (MQ=255) aGGCTCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCAcc < 1:1530093/51‑1 (MQ=255) cTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGcc < 1:565391/65‑1 (MQ=255) cTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGcc < 1:2432292/65‑1 (MQ=255) cTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGcc < 1:1990463/65‑1 (MQ=255) cTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTAt < 1:479268/68‑1 (MQ=255) cTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTAt < 1:415663/68‑1 (MQ=255) cTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTAt < 1:54336/68‑1 (MQ=255) cTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTAt < 1:267293/68‑1 (MQ=255) cTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTAt < 1:2474397/68‑1 (MQ=255) | GCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTAT > W3110S.gb/547771‑547890 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |