| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,728,624 | 0 | G | A | 70.0% | 38.5 / 21.4 | 30 | W84* (TGG→TGA) | nemA | N‑ethylmaleimide reductase, FMN‑linked |
| Reads supporting (aligned to +/- strand): ref base G (1/8); new base A (14/7); total (15/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.42e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAGGCAAAAGGATATGCAGGTGCGCCTGGCATCCATAGTCCGGAGCAAATTGCCGCATG‑GAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATAT > W3110S.gb/1728565‑1728665 | cAGGCAAAAGGATATGCAGGTGCGCCTGGCATCCATAGTCCGGAGCAAATTGCCGCATG‑AAAAAAAATCAc < 1:936106/71‑1 (MQ=255) gATATGCAGGTGCGCCTGGCATCCATAGTCCGGAGCAAATTGCCGCATG‑AAAAAAAATCACCGCTGGCGtt < 1:4026312/71‑1 (MQ=255) tGCAGGTGCGCCTGGCATCCATAGTCCGGAGCAAATTGCCGCATG‑AAAAAAAATCACCGCTGGCGtt < 1:2049597/67‑1 (MQ=255) tGCAGGTGCGCCTGGCATCCATAGTCCGGAGCAAATTGCCGCATG‑AAAAAAAATCACCGCTGGCGtt < 1:3505417/67‑1 (MQ=255) tGCGCCTGGCATCCATAGTCCGGAGCAAATTGCCGCATGAAAAAAAAATCACCGCTGGCGTTCATGCTGaa < 1:1346841/71‑1 (MQ=255) tGCGCCTGGCATCCATAGTCCGGAGCAAATTGCCGCATGAAAAAAAAATCACCGCTGGCGTTCATGCTGaa < 1:899817/71‑1 (MQ=255) tGCGCCTGGCATCCATAGTCCGGAGCAAATTGCCGCATGAAAAAAAAATCACCGCTGGCGTTCATGCTGaa < 1:3668563/71‑1 (MQ=255) gcgcCTGGCATCCATAGTCCGGAGCAAATTGCCGCATG‑GAAAAAAATCACCGCTGGCGTTCATGCTGaaaa > 1:3546366/1‑71 (MQ=255) gCATCCATAGTCCGGAGCAAATTGCCGCATG‑GAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCa < 1:133967/70‑1 (MQ=255) gCATCCATAGTCCGGAGCAAATTGCCGCATG‑GAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCa < 1:1557005/70‑1 (MQ=255) gCATCCATAGTCCGGAGCAAATTGCCGCATG‑GAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCa < 1:2260182/70‑1 (MQ=255) gCATCCATAGTCCGGAGCAAATTGCCGCATG‑GAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCa < 1:2390052/70‑1 (MQ=255) gCATCCATAGTCCGGAGCAAATTGCCGCATG‑GAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCa < 1:3071133/70‑1 (MQ=255) cATAGTCCGGAGCAAATTGCCGCATG‑AAAAAAAATCAc > 1:97735/1‑38 (MQ=255) cATAGTCCGGAGCAAATTGCCGCATG‑AAAAAAAATCAc > 1:3337013/1‑38 (MQ=255) cATAGTCCGGAGCAAATTGCCGCATG‑AAAAAAAATCAc > 1:2671755/1‑38 (MQ=255) cATAGTCCGGAGCAAATTGCCGCATG‑AAAAAAAATCAc > 1:2556414/1‑38 (MQ=255) cATAGTCCGGAGCAAATTGCCGCATG‑AAAAAAAATCAc > 1:2190505/1‑38 (MQ=255) cATAGTCCGGAGCAAATTGCCGCATG‑AAAAAAAATCAc > 1:1585342/1‑38 (MQ=255) aGTCCGGAGCAAATTGCCGCATG‑AAAAAAAATCACCGCTGGCg > 1:933208/1‑43 (MQ=255) aGTCCGGAGCAAATTGCCGCATG‑AAAAAAAATCACCGCTGGCGTTc > 1:3957034/1‑46 (MQ=255) aGTCCGGAGCAAATTGCCGCATG‑AAAAAAAATCACCGCTGGCGTTCa > 1:3820585/1‑47 (MQ=255) aGTCCGGAGCAAATTGCCGCATG‑AAAAAAAATCACCGCTGGCGTTCa > 1:64309/1‑47 (MQ=255) aGTCCGGAGCAAATTGCCGCATG‑AAAAAAAATCACCGCTGGCGTTCa > 1:1724713/1‑47 (MQ=255) gTCCGGAGCAAATTGCCGCATG‑AAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCatat > 1:2563264/1‑64 (MQ=255) gTCCGGAGCAAATTGCCGCATG‑AAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCata > 1:2785810/1‑63 (MQ=255) cGGAGCAAATTGCCGCATG‑AAAAAAAATCACCGCTGGCGTTCa > 1:2019254/1‑43 (MQ=255) tG‑GAAAAAAATCTCCGCTGGCGTTCATGCTGAAAATGGTCa < 1:2055465/41‑1 (MQ=255) tG‑GAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCa < 1:3550100/41‑1 (MQ=255) tG‑GAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCa < 1:2026497/41‑1 (MQ=255) ttgAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCa < 1:1288070/39‑1 (MQ=255) | CAGGCAAAAGGATATGCAGGTGCGCCTGGCATCCATAGTCCGGAGCAAATTGCCGCATG‑GAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATAT > W3110S.gb/1728565‑1728665 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |