Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,144,552	G→A	R465C (CGT→TGT)	rne ←	fused ribonucleaseE endoribonuclease and scaffold for formation of degradosome

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,144,552	0	G	A	100.0%	93.7 / NA	31	R465C (CGT→TGT)	rne	fused ribonucleaseE endoribonuclease and scaffold for formation of degradosome
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/11);  total (20/11)

ACGTGGTAGTGCGGGGTTTCCATCTGATCGTTTGGCACAATTACACAGCGCACACCGTCCTGACGAGTTTCAATGGCATTTACCGCAGAACGTTTTTCATTCAGCAGGTAAGAAGCGA  >  W3110S.gb/1144488‑1144605                                                                 |                                                      aCGTGGTAGTGCGGGGTTTCCATCTGATCGTTTGGCACAATTACACAGCGCACACCGTCCTGACAAGTTTc                                                 <  1:2947253/71‑1 (MQ=255) aCGTGGTAGTGCGGGGTTTCCATCTGATCGTTTGGCACAATTACACAGCGCACACCGTCCTGACAAGTTTc                                                 <  1:2682470/71‑1 (MQ=255) aCGTGGTAGTGCGGGGTTTCCATCTGATCGTTTGGCACAATTACACAGCGCACACCGTCCTGACAAGTTTc                                                 <  1:3264110/71‑1 (MQ=255) aCGTGGTAGTGCGGGGTTTCCATCTGATCGTTTGGCACAATTACACAGCGCACACCGTCCTGACAAGTTTc                                                 <  1:2489246/71‑1 (MQ=255) aCGTGGTAGTGCGGGGTTTCCATCTGATCGTTTGGCACAATTACACAGCGCACACCGTCCTGACAAGTTTc                                                 <  1:2481130/71‑1 (MQ=255)         gTGCGGGGTTTCCATCTGATCGTTTGGCACAATTACACAGCGCACACCGTCCTGACAAGTTTCAATGGCAt                                         >  1:4094045/1‑71 (MQ=255)         gTGCGGGGTTTCCATCTGATCGTTTGGCACAATTACACAGCGCACACCGTCCTGACAAGTTTCAATGGCAt                                         >  1:1289224/1‑71 (MQ=255)                 tttCCATCTGATCGTTTGGCACAATTACACAGCGCACACCGTCCTGACAAGtt                                                   <  1:3297382/53‑1 (MQ=255)                 tttCCATCTGATCGTTTGGCACAATTACACAGCGCACACCGTCCTGACAAGtt                                                   <  1:2525012/53‑1 (MQ=255)                 tttCCATCTGATCGTTTGGCACAATTACACAGCGCACACCGTCCTGACAAGtt                                                   <  1:1320709/53‑1 (MQ=255)                     cATCTGATCGTTTGGCACAATTACACAGCGCACACCGTCCTGACAAGTTTc                                                 <  1:556429/51‑1 (MQ=255)                           aTCGTTTGGCACAATTACACAGCGCACACCGTCCTGACAAGTTTCaa                                               >  1:3509125/1‑47 (MQ=255)                           aTCGTTTGGCACAATTACACAGCGCACACCGTCCTGACAAGTTTCaa                                               >  1:1490967/1‑47 (MQ=255)                           aTCGTTTGGCACAATTACACAGCGCACACCGTCCTGACAAGTTTCaa                                               >  1:585597/1‑47 (MQ=255)                           aTCGTTTGGCACAATTACACAGCGCACACCGTCCTGACAAGTTTCaa                                               >  1:3062312/1‑47 (MQ=255)                             cGTTTGGCACAATTACACAGCGCACACCGTCCTGACAAGTTTCAATGGCATTTACCGCAGAACGTTTttca                     >  1:456445/1‑71 (MQ=255)                             cGTTTGGCACAATTACACAGCGCACACCGTCCTGACAAGTTTCAATGGCATTTACCGCAGAACGTTTttca                     >  1:629881/1‑71 (MQ=255)                             cGTTTGGCACAATTACACAGCGCACACCGTCCTGACAAGTTTCAATGGCATTTACCGCAGAACGTTTttca                     >  1:244192/1‑71 (MQ=255)                              gTTTGGCACAATTACACAGCGCACACCGTCCTGACAAGTTTCAATGGCATTTACCg                                   >  1:763939/1‑56 (MQ=255)                              gTTTGGCACAATTACACAGCGCACACCGTCCTGACAAGTTTCAATGGCATTTACCg                                   >  1:1214019/1‑56 (MQ=255)                                ttGGCACAATTACACAGCGCACACCGTCCTGACAAGTTTCAATGGCATTTACCGCAGAACGTTTttcatt                   >  1:776693/1‑70 (MQ=255)                                ttGGCACAATTACACAGCGCACACCGTCCTGACAAGTTTCAATGGCATTTACCGCAGAACGTTTttcatt                   >  1:10899/1‑70 (MQ=255)                                ttGGCACAATTACACAGCGCACACCGTCCTGACAAGTTTCAATGGCATTTACCGCAGAACGTTTttcatt                   >  1:2529090/1‑70 (MQ=255)                                ttGGCACAATTACACAGCGCACACCGTCCTGACAAGTTTCAATGGCATTTACCGCAGAACGTTTttcatt                   >  1:1782838/1‑70 (MQ=255)                                ttGGCACAATTACACAGCGCACACCGTCCTGACAAGTTTCAATGGCATTTACCGCAGAACGTTAttcatt                   >  1:1870420/1‑70 (MQ=255)                                   gCACAATTACACAGCGCACACCGTCCTGACAAGTTTCAATGGCATTTACCGCAGAACGTTTTTCATTcagc               <  1:3670962/71‑1 (MQ=255)                                   gCACAATTACACAGCGCACACCGTCCTGACAAGTTTCAATGGCATTTACCGCAGAACGTTTTTCATTcagc               <  1:3383578/71‑1 (MQ=255)                                                  gcACACCGTCCTGACAAGTTTCAATGGCATTTACCGCAGAACGTTTTTCATTCAGCAGGTAAGAAGc    >  1:3848959/1‑67 (MQ=255)                                                  gcACACCGTCCTGACAAGTTTCAATGGCATTTACCGCAGAACGTTTTTCATTCAGCAGGTAAGAAGc    >  1:3005299/1‑67 (MQ=255)                                                            cTGACAAGTTTCAATGGCATTTACCGCAGAACGTTTTTCATTCAGCAGGTAAGAAGCGa  >  1:483902/1‑59 (MQ=255)                                                            cTGACAAGTTTCAATGGCATTTACCGCAGAACGTTTTTCATTCAGCAGGTAAGAAGCGa  >  1:3001256/1‑59 (MQ=255)                                                                 |                                                      ACGTGGTAGTGCGGGGTTTCCATCTGATCGTTTGGCACAATTACACAGCGCACACCGTCCTGACGAGTTTCAATGGCATTTACCGCAGAACGTTTTTCATTCAGCAGGTAAGAAGCGA  >  W3110S.gb/1144488‑1144605

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑