Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 1,842,575 Δ2 bp coding (542‑543/621 nt) ynjF ← predicted phosphatidyl transferase, inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,842,5750G.100.0% 337.5 / NA 75coding (543/621 nt)ynjFpredicted phosphatidyl transferase, inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (0/75);  total (0/75)
*W3110S.gb1,842,5760A.100.0% 337.5 / NA 75coding (542/621 nt)ynjFpredicted phosphatidyl transferase, inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (0/75);  total (0/75)

TAAAGGTCGTCATCCAGCACAACGCACCGAAGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAT  >  W3110S.gb/1842547‑1842617
                            ||                                         
tAAAGGTCGTCATCCAGCACATCGCACC‑‑AGATCGACGCAAACCACGCAAACCATGGAGGGAATAAACAt  <  1:633401/69‑1 (MQ=255)
tAAAGGTCGTCATCCAGCACAACGCACC‑‑AGATCCTCGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:826494/69‑1 (MQ=255)
tAAAGGTCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCTCGCAAACCATGCAGGGAATAAACAt  <  1:270780/69‑1 (MQ=255)
tAAAGGTCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCACTCAAACCATGCAGGGAATAAACAt  <  1:930188/69‑1 (MQ=255)
tAAAGGTCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:662557/69‑1 (MQ=255)
tAAAGGTCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:695820/69‑1 (MQ=255)
tAAAGGTCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:659968/69‑1 (MQ=255)
tAAAGGTCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:636550/69‑1 (MQ=255)
tAAAGGTCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:59537/69‑1 (MQ=255)
tAAAGGTCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:531828/69‑1 (MQ=255)
tAAAGGTCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:503507/69‑1 (MQ=255)
tAAAGGTCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:464787/69‑1 (MQ=255)
tAAAGGTCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:445005/69‑1 (MQ=255)
tAAAGGTCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:436099/69‑1 (MQ=255)
tAAAGGTCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:383635/69‑1 (MQ=255)
tAAAGGTCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:37105/69‑1 (MQ=255)
tAAAGGTCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:1013998/69‑1 (MQ=255)
tAAAGGTCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:291558/69‑1 (MQ=255)
tAAAGGTCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:670473/69‑1 (MQ=255)
tAAAGGTCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:327042/69‑1 (MQ=255)
tAAAGGTCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:721603/69‑1 (MQ=255)
tAAAGGTCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:742077/69‑1 (MQ=255)
tAAAGGTCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:770954/69‑1 (MQ=255)
tAAAGGTCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:834720/69‑1 (MQ=255)
tAAAGGTCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:842672/69‑1 (MQ=255)
tAAAGGTCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:862647/69‑1 (MQ=255)
tAAAGGTCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:86562/69‑1 (MQ=255)
tAAAGGTCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:86618/69‑1 (MQ=255)
tAAAGGTCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:874584/69‑1 (MQ=255)
tAAAGGTCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:943959/69‑1 (MQ=255)
tAAAGGTCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:955545/69‑1 (MQ=255)
tAAAGGTCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:965155/69‑1 (MQ=255)
tAAAGGTCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:970367/69‑1 (MQ=255)
tAAAGGTCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:98548/69‑1 (MQ=255)
tAAAGGTCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:1536066/69‑1 (MQ=255)
tAAAGGTCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:1202534/69‑1 (MQ=255)
tAAAGGTCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:12865/69‑1 (MQ=255)
tAAAGGTCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:1295257/69‑1 (MQ=255)
tAAAGGTCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:1302463/69‑1 (MQ=255)
tAAAGGTCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:1202256/69‑1 (MQ=255)
tAAAGGTCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:119933/69‑1 (MQ=255)
tAAAGGTCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:1157348/69‑1 (MQ=255)
tAAAGGTCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:1382234/69‑1 (MQ=255)
tAAAGGTCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:1416344/69‑1 (MQ=255)
tAAAGGTCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:1141415/69‑1 (MQ=255)
tAAAGGTCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:1491406/69‑1 (MQ=255)
tAAAGGTCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:270568/69‑1 (MQ=255)
tAAAGGTCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:1505585/69‑1 (MQ=255)
tAAAGGTCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:1518591/69‑1 (MQ=255)
tAAAGGTCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:1521585/69‑1 (MQ=255)
tAAAGGTCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:106397/69‑1 (MQ=255)
tAAAGGTCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:1051660/69‑1 (MQ=255)
tAAAGGTCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:267617/69‑1 (MQ=255)
tAAAGGTCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:255781/69‑1 (MQ=255)
tAAAGGTCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:250515/69‑1 (MQ=255)
tAAAGGTCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:1055234/69‑1 (MQ=255)
tAAAGGTCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:232091/69‑1 (MQ=255)
tAAAGGTCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:22656/69‑1 (MQ=255)
tAAAGGTCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:1119961/69‑1 (MQ=255)
tAAAGGTCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:209212/69‑1 (MQ=255)
tAAAGGTCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:193890/69‑1 (MQ=255)
tAAAGGTCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:171154/69‑1 (MQ=255)
tAAAGGTCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:1594000/69‑1 (MQ=255)
tAAAGGTCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:1583640/69‑1 (MQ=255)
tAAAGGTCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:1572869/69‑1 (MQ=255)
tAAAGGCCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:842167/69‑1 (MQ=255)
 aaaGGTCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:712630/68‑1 (MQ=255)
 aaaGGTCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:1360685/68‑1 (MQ=255)
 aaaGGTCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:804060/68‑1 (MQ=255)
 aaaGGTCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:724764/68‑1 (MQ=255)
 aaaGGTCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:217734/68‑1 (MQ=255)
 aaaGGTCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:378150/68‑1 (MQ=255)
 aaaGGTCGTCATCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:279556/68‑1 (MQ=255)
           aTCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:771499/58‑1 (MQ=255)
           aTCCAGCACAACGCACC‑‑AGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAt  <  1:442037/58‑1 (MQ=255)
                            ||                                         
TAAAGGTCGTCATCCAGCACAACGCACCGAAGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACAT  >  W3110S.gb/1842547‑1842617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑