Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,853,352	A→G	S76S (TCT→TCC)	envC ←	protease with a role in cell division

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,853,352	0	A	G	100.0%	181.4 / NA	50	S76S (TCT→TCC)	envC	protease with a role in cell division
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (50/0);  total (50/0)

CAATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCTTCAGAGATCG  >  W3110S.gb/3853291‑3853359                                                              |        caATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCTTCGgagagca  >  1:592222/1‑66 (MQ=255) caATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCTTCGgagagca  >  1:102617/1‑66 (MQ=255) caATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCTTCGgagagca  >  1:205861/1‑66 (MQ=255) caATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCTTCGgagagca  >  1:235891/1‑66 (MQ=255) caATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCTTCGgagagca  >  1:250563/1‑66 (MQ=255) caATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCTTCGgagagca  >  1:343738/1‑66 (MQ=255) caATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCTTCGgagagca  >  1:374253/1‑66 (MQ=255) caATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCTTCGgagagca  >  1:416422/1‑66 (MQ=255) caATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCTTCGgagagca  >  1:482274/1‑66 (MQ=255) caATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCTTCGgagagca  >  1:492168/1‑66 (MQ=255) caATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCTTCGgagagca  >  1:530240/1‑66 (MQ=255) caATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCTTCGgagagca  >  1:584606/1‑66 (MQ=255) caATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCTTCGgagagca  >  1:590371/1‑66 (MQ=255) caATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCTTCGgagagca  >  1:1596004/1‑66 (MQ=255) caATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCTTCGgagagca  >  1:649007/1‑66 (MQ=255) caATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCTTCGgagagca  >  1:680067/1‑66 (MQ=255) caATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCTTCGgagagca  >  1:728596/1‑66 (MQ=255) caATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCTTCGgagagca  >  1:81930/1‑66 (MQ=255) caATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCTTCGgagagca  >  1:869264/1‑66 (MQ=255) caATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCTTCGgagagca  >  1:904/1‑66 (MQ=255) caATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCTTCGgagagca  >  1:934058/1‑66 (MQ=255) caATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCTTCGgagagca  >  1:96040/1‑66 (MQ=255) caATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCTTCGgagagca  >  1:968006/1‑66 (MQ=255) caATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCTTCGgagagca  >  1:983887/1‑66 (MQ=255) caATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCTTCGgagagca  >  1:993274/1‑66 (MQ=255) caATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCTTCGgagagca  >  1:1316971/1‑66 (MQ=255) caATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCTTCGgagagca  >  1:1040575/1‑66 (MQ=255) caATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCTTCGgagagca  >  1:1094612/1‑66 (MQ=255) caATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCTTCGgagagca  >  1:113860/1‑66 (MQ=255) caATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCTTCGgagagca  >  1:1155052/1‑66 (MQ=255) caATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCTTCGgagagca  >  1:1170833/1‑66 (MQ=255) caATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCTTCGgagagca  >  1:1171667/1‑66 (MQ=255) caATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCTTCGgagagca  >  1:1183104/1‑66 (MQ=255) caATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCTTCGgagagca  >  1:1195635/1‑66 (MQ=255) caATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCTTCGgagagca  >  1:1223717/1‑66 (MQ=255) caATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCTTCGgagagca  >  1:1242469/1‑66 (MQ=255) caATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCTTCGgagagca  >  1:1253876/1‑66 (MQ=255) caATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCTTCGgagagca  >  1:1282977/1‑66 (MQ=255) caATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCTTCGgagagca  >  1:1585015/1‑66 (MQ=255) caATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCTTCGgagagca  >  1:1337889/1‑66 (MQ=255) caATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCTTCGgagagca  >  1:1380952/1‑66 (MQ=255) caATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCTTCGgagagca  >  1:138728/1‑66 (MQ=255) caATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCTTCGgagagca  >  1:1412536/1‑66 (MQ=255) caATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCTTCGgagagca  >  1:1423813/1‑66 (MQ=255) caATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCTTCGgagagca  >  1:1428847/1‑66 (MQ=255) caATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCTTCGgagagca  >  1:1430315/1‑66 (MQ=255) caATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCTTCGgagagca  >  1:1478117/1‑66 (MQ=255) caATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCTTCGgagagca  >  1:151223/1‑66 (MQ=255) caATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCTTCGgagagca  >  1:1580877/1‑66 (MQ=255) caATCTGTTTATTGAGTTGACTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCTTCGgagagca  >  1:551193/1‑66 (MQ=255)                                                              |        CAATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCTTCAGAGATCG  >  W3110S.gb/3853291‑3853359

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑