| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,641,490:1 | +G | 23.7% | coding (113/1425 nt) | creC → | sensory histidine kinase in two‑component regulatory system with CreB or PhoB, regulator of the CreBC regulon |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,641,490 | 1 | . | G | 23.7% | 57.3 / 43.2 | 38 | coding (113/1425 nt) | creC | sensory histidine kinase in two‑component regulatory system with CreB or PhoB, regulator of the CreBC regulon |
| Reads supporting (aligned to +/- strand): ref base . (13/16); new base G (3/6); total (16/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.06e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAAC‑GGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGG > W3110S.gb/4641423‑4641557 | gcggtggcAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAAC‑g > 1:3014212/1‑69 (MQ=255) gcAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAAC‑gg > 1:2476141/1‑64 (MQ=255) ccTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGGAGGGGACGtt < 1:2764283/71‑1 (MQ=255) cTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGGAGGGGACGTTg < 1:702707/71‑1 (MQ=255) tCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAAC‑GGAGGGGACGTTGATCGa > 1:1654089/1‑70 (MQ=255) cGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAAC‑GGAGGGGACGTTGATCGac < 1:3495013/70‑1 (MQ=255) gTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAAC‑gg < 1:96093/52‑1 (MQ=255) cTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAAC‑ggga > 1:3113445/1‑49 (MQ=255) ggCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAAC‑GGAGGGGACGTTGATCGaca > 1:4236610/1‑65 (MQ=255) aTTTTTGTCAAAGAAGTTAACCCGGGCGTGCGAAGAGCAACGGGAGGGGACGTTGATc < 1:1166116/58‑1 (MQ=255) aTTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAAC‑GGAGGGGACGTTGATCGACACCGCAACGtt < 1:119610/71‑1 (MQ=255) tttttGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAAC‑GGAGGGGACg < 1:4442759/50‑1 (MQ=255) ttttGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAAC‑GGAGGGGACGTTGATc < 1:2703494/55‑1 (MQ=255) ttttGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAAC‑GGAGGGGACGTTGATCGACACCGCAACGTTGc > 1:3373216/1‑71 (MQ=255) tttGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAAc > 1:262731/1‑38 (MQ=255) tttGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGGAGGGGACGTTGAt > 1:3995661/1‑54 (MQ=255) tttGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAAC‑GGa > 1:4109331/1‑41 (MQ=255) tttGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAAC‑GGAGGGGACGTTGATCGACACCGCAAc > 1:2855445/1‑65 (MQ=255) tttGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAAC‑GGAGGGGACGTTGATCGACACCGCAACGTTGCt > 1:2405645/1‑71 (MQ=255) tttGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAAC‑GGAGGGGACGTTGATCGACACCGCAACGTTGCt > 1:2847228/1‑71 (MQ=255) tttGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAAC‑GGAGGGGACGTTGATCGACACCGCAACGTTGCt > 1:4344218/1‑71 (MQ=255) ttGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGGAGGGGACGTTGAt > 1:1441167/1‑53 (MQ=255) aaaGAAGTTAAACCGGGCGTGCGAAGAGCAAC‑GGAGGGGACGTTGATCGa > 1:4317005/1‑50 (MQ=255) aagaagTTAAACCGGGCGTGCGAAGAGCAAC‑GGAGGGGACGTTGATCGACACCGCAACGTTGCTgg < 1:1535266/66‑1 (MQ=255) gaagTTAAACCGGGCGTGCGAAGAGCAACGGGAGGGGACGTTGATCGa < 1:3529456/48‑1 (MQ=255) aagTTAAACCGGGCGTGCGAAGAGCAAC‑GGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTg < 1:526347/71‑1 (MQ=255) gTTAAACCGGGCGTGCGAAGAGCAACGGGAGGGGACGTTGATCGACACCGCAACGTTGCTgg < 1:1582320/62‑1 (MQ=255) gTTAAACCGGGCGTGCGAAGAGCAAC‑GGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGgc < 1:3209038/71‑1 (MQ=255) ttAAACCGGGCGTGCGAAGAGCAAC‑GGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCgg < 1:389701/63‑1 (MQ=255) tAAACCGGGCGTGCGAAGAGCAACGGGAGGGGACGTTGATCGacac > 1:3327565/1‑46 (MQ=255) aaaCCGGGCGTGCGAAGAGCAAC‑GGAGGGGACGTTGATCGACACCGCAACGt > 1:973285/1‑52 (MQ=255) aaaCCGGGCGTGCGAAGAGCAAC‑GGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGcgcg > 1:4533478/1‑71 (MQ=255) aaCCGGGCGTGCGAAGAGCAAC‑GGAGGGGACGTTGATCGAAACCGCAACGTTGCTGGCGGAGCTGGcgcg < 1:1991658/70‑1 (MQ=255) cGGGCGTGCGAAGAGCAAC‑GGAGGGGACGTTGATCGACCCCGCAACGTTGCTGGCGGAGCTGGCGCGt > 1:1336774/1‑68 (MQ=255) ggCGTGCGAAGAGCAAC‑GGAGTGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGa < 1:2126260/71‑1 (MQ=255) gTGCGAAGAGCAACGGGAGGGGACGTTGATCGACACCGCAACGTTGCTgg < 1:3814844/50‑1 (MQ=255) tGCGAAGAGCAAC‑GGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTg < 1:3415274/71‑1 (MQ=255) cGAAGAGCAAC‑GGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGct < 1:2191440/71‑1 (MQ=255) gagCAAC‑GGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGctctct < 1:777470/71‑1 (MQ=255) agCAAC‑GGAGGGGACGTTGATCGACACCGCAACGTTGCt < 1:254093/39‑1 (MQ=255) gCAAC‑GGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTg < 1:2661454/70‑1 (MQ=255) cAAC‑GGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTggg < 1:947520/71‑1 (MQ=255) cAAC‑GGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTggg > 1:1279666/1‑71 (MQ=255) | GCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAAC‑GGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGG > W3110S.gb/4641423‑4641557 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |