| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,788,749 | C→T | 23.1% | P38S (CCG→TCG) | ygaF → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,788,749 | 0 | C | T | 23.1% | 74.5 / 19.4 | 39 | P38S (CCG→TCG) | ygaF | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (15/15); new base T (6/3); total (21/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.64e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01 | |||||||||||
TCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCT > W3110S.gb/2788680‑2788819 | tCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTTGAAAAAGAGTCCGcccc > 1:1491669/1‑71 (MQ=255) cGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCTCg > 1:3943773/1‑71 (MQ=255) ccATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGcccc > 1:4172244/1‑64 (MQ=255) ccATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGt > 1:4090732/1‑71 (MQ=255) ccATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGt > 1:1801427/1‑71 (MQ=255) cATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTc < 1:3561878/71‑1 (MQ=255) cAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCTCGGcc > 1:2948275/1‑63 (MQ=255) cAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCTCGGCCTGTCAc > 1:127075/1‑69 (MQ=255) cAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCa > 1:2421576/1‑68 (MQ=255) cAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCa < 1:3874954/71‑1 (MQ=255) aCTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCt < 1:1037221/62‑1 (MQ=255) aCTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCt < 1:2713503/62‑1 (MQ=255) cTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGAc > 1:4494040/1‑71 (MQ=255) cTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGAc > 1:3134917/1‑71 (MQ=255) gattgatGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGcc > 1:1047995/1‑58 (MQ=255) gattgatGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACgg > 1:1244053/1‑71 (MQ=255) atGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCcac < 1:146652/71‑1 (MQ=255) tGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCcac > 1:2652899/1‑70 (MQ=255) tCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCt > 1:4088520/1‑51 (MQ=255) tCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCacaac < 1:853138/71‑1 (MQ=255) tCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCacaac < 1:1815451/71‑1 (MQ=255) gCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCTCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGAt > 1:3693672/1‑71 (MQ=255) cATTGCGTTGCTGGAAAAAGAGTCCGCCTCGGCCTGTCACCAGACGGGCCACAACAg < 1:119976/57‑1 (MQ=255) cATTGCGTTGCTGGAAAAAGAGTCCGCCTCGGCCTGTCACCAGACGGGCCACAACAg < 1:2861830/57‑1 (MQ=255) cATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCt < 1:1202321/35‑1 (MQ=255) ttGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGa < 1:3098969/42‑1 (MQ=255) ttGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATc < 1:2123850/65‑1 (MQ=255) ttGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGcc < 1:2889440/71‑1 (MQ=255) ttGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGcc > 1:4149148/1‑71 (MQ=255) gCGTTGCTGGAAAAAGAGTCCGCCTCGGCCTGTCACCAGACGGGCcac > 1:445474/1‑48 (MQ=255) gCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCa > 1:4571879/1‑35 (MQ=255) gCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCgg > 1:3293561/1‑71 (MQ=255) ttGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCggg < 1:2756498/69‑1 (MQ=255) cTGGAAAAAGAGTCCGCCTCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCt < 1:2405928/70‑1 (MQ=255) cTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCt < 1:3344603/70‑1 (MQ=255) gAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTAt < 1:4187847/69‑1 (MQ=255) aaaaaGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGAt > 1:651596/1‑52 (MQ=255) aaaaaGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCgggg > 1:3972478/1‑63 (MQ=255) gagTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGcc > 1:324871/1‑71 (MQ=255) cGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCa < 1:3525094/71‑1 (MQ=255) gCCTCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAg > 1:1989703/1‑71 (MQ=255) ccGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCt < 1:2910574/71‑1 (MQ=255) ccGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCt < 1:2097225/71‑1 (MQ=255) | TCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCT > W3110S.gb/2788680‑2788819 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |