| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,816,575:1 | +G | 28.6% | coding (995/2262 nt) | katE → | hydroperoxidase HPII(III) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,816,575 | 1 | . | G | 28.6% | 12.7 / 14.1 | 14 | coding (995/2262 nt) | katE | hydroperoxidase HPII(III) |
| Reads supporting (aligned to +/- strand): ref base . (7/3); new base G (2/2); total (9/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.80e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AACTCACCGGACGTGACCCGGACTTCCACCGCCGCGAGTTGTGGGAAGCCATTGAAGCAGGCGATTTTCC‑GGAATACGAACTGGGCTTCCAGTTGATTCCTGAAGAAGATGAATTCAAGTTCGACTTCGATCT > W3110S.gb/1816506‑1816638 | aaCTCACCGTACGTGACCCGGACTTCCACCGCCGCGAGTTGTGGGAAGCCATTGAAGCAGGCGATTTTCC‑g < 1:3122074/71‑1 (MQ=255)aaCTCACCGGACGTGACCCGGCCTTCCACCGCCGCGAGTTGTGGGAAGCCATTGAAGCAGGCGATTTTCC‑g > 1:335938/1‑71 (MQ=255)aaCTCACCGGACGTGACCCGGACTTCCACCGCCGCGAGTTGTGGGAAGCCATTGAAGCAGGCGATTTTCC‑g < 1:1889995/71‑1 (MQ=255) aCTCACCGGACGTGACCCGGACTTCCACCGCCGCGAGTTGTGGGAAGCCATTGAAGCAGGCGATTTTCC‑gg < 1:2159789/71‑1 (MQ=255) aCTCACCGGACGTGACCCGGACTTCCACCGCAGCGAGTTGTGGGAAGCCATTGAAGCAGGCGATTTTCC‑g < 1:2564468/70‑1 (MQ=255) aGTTGTGGGAAGCCATTGAAGCAGGCGATTTTCC‑GGAATACGAACTGGGCTTCCAGTTGATTCCTgaagaa < 1:2042957/71‑1 (MQ=255) ttGTGGGAAGCCATTGAAGCAGGCGATTTTCC‑ggg < 1:2487647/35‑2 (MQ=255) ttGTGGGAAGCCATTGAAGCAGGCGATTTTCC‑ggg < 1:670925/35‑2 (MQ=255) ttGTGGGAAGCCATTGAAGCAGGCGATTTTCC‑ggg < 1:371075/35‑2 (MQ=255) ttGTGGGAAGCCATTGAAGCAGGCGATTTTCC‑ggg < 1:3579953/35‑2 (MQ=255) ttGTGGGAAGCCATTGAAGCAGGCGATTTTCC‑ggg < 1:1798565/35‑2 (MQ=255) gtgGGAAGCCATTGAAGCAGGCGATTTTCC‑GGAATACGAACTGGGCTTCCAGTTGATTCCTGAAGAAGAt > 1:1940798/1‑70 (MQ=255) aggcATTGAAGCAGGCGATTTTCC‑GGAATACGAACTGGGCTTCCAGTTGATTCCTGAAGAAGATGAATTc > 1:1743900/4‑70 (MQ=255) aGCCATTGAAGCAGGCGATTTTGC‑GGAATACGAACTGGGCTTCCAGTTGATTCCTGAAGAAGATGAATTc > 1:2894108/1‑70 (MQ=255) ctttGAAGCAGGCGATTTTCC‑GGAATACGAACTGGGCTTCCAGTTGATTCCTGAACAAGATGAATTc < 1:2234960/65‑1 (MQ=255) ttGAAGCAGGCGATTTTCCGGGAATACGAACTGGGCTTCCAGTTGATTCCTGAAGAAGATGa < 1:1085389/62‑1 (MQ=255) gAAGCAGGCGATTTTCCGGGAATACGAACTGGGCTTCCAGTTGATTCCTGAAGAAGATGAAt > 1:3470908/1‑62 (MQ=255) gAAGCAGGCGATTTTCCGGGAATACGAACTGGGCTTCCAGTTGATTCCTGAAGAAGATGAAt > 1:1097708/1‑62 (MQ=255) gAAGCAGGCGATTTTCC‑GGAATACGAACTGGGCTTCCAGTTGATTCCTGAAGAAGATGAATTCAAGTTCg < 1:1103787/70‑1 (MQ=255) gAAGCAGGCGATTTTCC‑GGAATACGAACTGGGCTTCCAGTTGATTCCTGAAGAAGATGAATTCAAGTTCGa > 1:3181244/1‑71 (MQ=255) gAAGCAGGCGATTTTCC‑GGAATACGAACTGGGCTTCCAGTTGATTCCTGAAGAAGATGAATTCAAGTTCGa > 1:1172048/1‑71 (MQ=255) gAAGCAGGCGATTTTCC‑GGAATACGAACTGGGCTTCCAGTTGATTCCTGAAGAAGATGAATTCAAGTTCGa > 1:3808610/1‑71 (MQ=255) gATTTTCC‑GGAATACGAACTGGGCTTCCAGTTGATTCCTGAAGAAGATGAATTCAAGTTCGACTTCGAtct > 1:1221464/1‑71 (MQ=255) aTTTTCCGGGAATACGAACTGGGCTTCCAGTTGATTCCTGAAGAAGATGAATTCAAGTTCGACTTCGAtct < 1:3345475/71‑1 (MQ=255) | AACTCACCGGACGTGACCCGGACTTCCACCGCCGCGAGTTGTGGGAAGCCATTGAAGCAGGCGATTTTCC‑GGAATACGAACTGGGCTTCCAGTTGATTCCTGAAGAAGATGAATTCAAGTTCGACTTCGATCT > W3110S.gb/1816506‑1816638 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |