| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,788,749 | C→T | 31.8% | P38S (CCG→TCG) | ygaF → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,788,749 | 0 | C | T | 31.8% | 24.7 / 15.3 | 22 | P38S (CCG→TCG) | ygaF | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (6/9); new base T (4/3); total (10/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.52e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01 | |||||||||||
CCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTAC > W3110S.gb/2788684‑2788805 | ccGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCTCGGcc > 1:2890674/1‑71 (MQ=255)ccGCCATGCAACAGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCTCGGcc > 1:2182678/1‑71 (MQ=255) cGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCTCGGCCt > 1:2096021/1‑71 (MQ=255) ccATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGt < 1:3362340/71‑1 (MQ=255) ccATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGt < 1:2471956/71‑1 (MQ=255) cAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGcccc > 1:3392847/1‑59 (MQ=255) cAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCAc > 1:1127333/1‑69 (MQ=255) cAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCAc > 1:3648358/1‑69 (MQ=255) gattgatGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACgg > 1:1953647/1‑71 (MQ=255) ttgatGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGccc > 1:2070931/1‑51 (MQ=255) ttgatGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGccc > 1:2644439/1‑51 (MQ=255) ttgatGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGccc > 1:3129137/1‑51 (MQ=255) ttgatGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCTCGGCCt < 1:1931668/57‑1 (MQ=255) tCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAg > 1:2695249/1‑69 (MQ=255) cccGCATTGCGTTGCTGGAAAAAGAGTCCGCCTCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATc < 1:3839579/71‑1 (MQ=255) cGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCa > 1:2445320/1‑71 (MQ=255) gCATTGCGTTGCTGGAAAAAGAGTCCGCCTCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCAt > 1:2329673/1‑71 (MQ=255) gCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGa > 1:29189/1‑45 (MQ=255) cATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCAc < 1:893668/40‑1 (MQ=255) gCTGGAAAAAGAGTCCGCCTCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTc < 1:1257698/70‑1 (MQ=255) cTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCt < 1:1067423/70‑1 (MQ=255) gAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCcaca < 1:1900089/40‑1 (MQ=255) gAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTAt < 1:266142/69‑1 (MQ=255) gAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTAt < 1:1579536/69‑1 (MQ=255) gAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTa < 1:3695366/71‑1 (MQ=255) aaaaaGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTac < 1:25458/71‑1 (MQ=255) | CCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTAC > W3110S.gb/2788684‑2788805 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |