| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,125,813 | C→T | 35.3% | P152L (CCT→CTT) | rpe → | D‑ribulose‑5‑phosphate 3‑epimerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,125,813 | 0 | C | T | 35.3% | 74.0 / 65.5 | 68 | P152L (CCT→CTT) | rpe | D‑ribulose‑5‑phosphate 3‑epimerase |
| Reads supporting (aligned to +/- strand): ref base C (26/18); new base T (13/11); total (39/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.99e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATGGATAAGCTGGATGTGATCCTGCTGATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGACTAG > W3110S.gb/4125746‑4125881 | aTGGATAAGCTGGATGTGATCCTGCTGATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCAttcttc > 1:1006326/1‑70 (MQ=255)aTGGATAAGCTGGATGTGATCCTGCTGATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCTTCa < 1:150102/71‑1 (MQ=255)aTGGATAAGCTGGATGTGATCCTGCTGATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTc > 1:4040971/1‑70 (MQ=255) gATAAGCTGGATGTGATCCTGCTGATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCTTCaaa < 1:1059307/70‑1 (MQ=255) gATAAGCTGGATGTGATCCTGCTGATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCTTCaaa > 1:1671282/1‑70 (MQ=255) aTAAGCTGGATGTGATCCTGCTGATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAaca < 1:3141587/71‑1 (MQ=255) tAAGCTGGATGTGATCCTGCTGATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCTTCAAaca < 1:3388535/70‑1 (MQ=255) aaGCTGGATGTGATCCTGCTGATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACt > 1:414135/1‑71 (MQ=255) gATGTGATCCTGCTGATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCTTCAAACACTGGATa > 1:2248215/1‑70 (MQ=255) gATGTGATCCTGCTGATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAaca > 1:2910649/1‑63 (MQ=255) gATGTGATCCTGCTGATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAaca > 1:3936247/1‑63 (MQ=255) gATGTGATCCTGCTGATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATaa < 1:3853747/71‑1 (MQ=255) gATGTGATCCTGCTGATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATa < 1:3709870/70‑1 (MQ=255) tgATCCTGCTGATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATaaa > 1:241813/1‑68 (MQ=255) tgATCCTGCTGATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATAAACTg > 1:2347721/1‑71 (MQ=255) ctgctgATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCTTCAAacac < 1:492376/55‑1 (MQ=255) ctgctgATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCTTCAAACACTGGATAAACTGcgcg < 1:2043203/70‑1 (MQ=255) ctgATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAg < 1:3227241/70‑1 (MQ=255) tgATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTa > 1:822912/1‑71 (MQ=255) gTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCTTCAAACACTGGATAAACTGCGCGAAGTACGTc > 1:217383/1‑71 (MQ=255) gTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCaaa > 1:3187313/1‑44 (MQ=255) gTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCaaa > 1:651708/1‑44 (MQ=255) cGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGcc > 1:623965/1‑71 (MQ=255) cGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGcc > 1:2965065/1‑71 (MQ=255) gTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATa < 1:2153402/49‑1 (MQ=255) gTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATa < 1:1877801/49‑1 (MQ=255) gTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATa < 1:1474222/49‑1 (MQ=255) tCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCTTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGt < 1:3093418/71‑1 (MQ=255) tCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCTTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGt < 1:11781/71‑1 (MQ=255) aaCCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTAt > 1:746538/1‑71 (MQ=255) aaCCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTAt > 1:2591666/1‑71 (MQ=255) cccTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATcg < 1:2807194/71‑1 (MQ=255) cTGGTTTCGGCGGTCAGTCTTTCATTCTTCAAaca > 1:497168/1‑35 (MQ=255) cTGGTTTCGGCGGTCAGTCTTTCATTCTTCAAACACt > 1:1593583/1‑37 (MQ=255) cTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATcgac < 1:1093850/71‑1 (MQ=255) cTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATcgac < 1:1559391/71‑1 (MQ=255) cTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATcgac < 1:3987414/71‑1 (MQ=255) tGGTTTCGGCGGTCAGTCTTTCATTCTTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATcgac > 1:3666950/1‑70 (MQ=255) tGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATa < 1:1970680/41‑1 (MQ=255) tGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACAATGGATa < 1:527325/41‑1 (MQ=255) ggTTTCGGCGGTCAGTCTTTCATTCTTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATcgac > 1:3196070/1‑69 (MQ=255) tttCGGCGGTCAGTCTTTCATTCTTCAAACACTGGATAAACTGcgcg < 1:3230591/47‑1 (MQ=255) tttCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATcgacga < 1:3008927/69‑1 (MQ=255) ttCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTc > 1:511992/1‑71 (MQ=255) tCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATa < 1:969552/36‑1 (MQ=255) ggTCAGTCTTTCATTCTTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCtt < 1:3467618/71‑1 (MQ=255) tCAGTCTTTCATTCTTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTg < 1:514640/71‑1 (MQ=255) aGTCTTTCATTCTTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGa > 1:3186658/1‑70 (MQ=255) aGTCTTTCATTCTTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGa < 1:299288/70‑1 (MQ=255) aGTCTTTCATTCTTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGa > 1:1593375/1‑70 (MQ=255) aGTCTTTCATTCTTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGa > 1:692075/1‑70 (MQ=255) gTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAg < 1:1431271/38‑1 (MQ=255) gTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAg < 1:2642473/38‑1 (MQ=255) tCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACAt < 1:1822350/71‑1 (MQ=255) cTTTCATTCCTCAAACACTGGATAAACTGCGCGAAg > 1:2970608/1‑36 (MQ=255) cTTTCATTCCTCAAACACTGGATAAACTGCGCGAAg > 1:1868758/1‑36 (MQ=255) cTTTCATTCCTCAAACACTGGATAAACTGCGCGAAg > 1:1745166/1‑36 (MQ=255) cTTTCATTCCTCAAACACTGGATAAACTGCGCGAAg > 1:1595194/1‑36 (MQ=255) cTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACAtt > 1:1734715/1‑71 (MQ=255) tttCATTCTTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACAtt > 1:262493/1‑70 (MQ=255) tttCATTCTTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTc > 1:524103/1‑71 (MQ=255) tttCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTg > 1:1096742/1‑59 (MQ=255) tttCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTg > 1:1930719/1‑59 (MQ=255) tttCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTg > 1:1432677/1‑59 (MQ=255) tttCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTc > 1:161220/1‑71 (MQ=255) tCATTCTTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACa > 1:3459135/1‑66 (MQ=255) tCATTCCTCAAACACTGGATAAACTGCGCGAAGTa > 1:412353/1‑35 (MQ=255) tCATTCCTCAAACACTGGATAAACTGCGCGAAGTa > 1:975183/1‑35 (MQ=255) tCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGa > 1:1842880/1‑71 (MQ=255) ttcttcAAACACTGGATAAACTGCGCGAAGTACGTc < 1:62488/36‑1 (MQ=255) ttcttcAAACACTGGATAAACTGCGCGAAGTACGTc < 1:813500/36‑1 (MQ=255) ccTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGACTAg < 1:1210677/70‑1 (MQ=255) | ATGGATAAGCTGGATGTGATCCTGCTGATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGACTAG > W3110S.gb/4125746‑4125881 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |