| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 70,740 | T→G | 90.3% | G118G (GGT→GGG) | araC → | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 70,740 | 0 | T | G | 90.3% | 475.0 / 16.8 | 150 | G118G (GGT→GGG) | araC | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base T (7/8); new base G (81/54); total (88/62) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.09e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 168 total aligned reads displayed.
GGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATAC‑GGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGCC > W3110S.gb/70671‑70806 | ggTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATAC‑GGGtt < 1:1749250/71‑1 (MQ=255)ggTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCCTAACTGGCCGTCAATATTTGCCAATAC‑GGGtt < 1:2402597/71‑1 (MQ=255) ttACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATAC‑GGGgttct > 1:2425313/1‑71 (MQ=255) ttACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATAC‑GGGgttct > 1:376078/1‑71 (MQ=255) tACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATAC‑GGGtttct < 1:3646029/70‑1 (MQ=255) tACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATAC‑GGGgttctt > 1:33685/1‑71 (MQ=255) aCTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATAC‑GGGgttctt < 1:2599715/70‑1 (MQ=255) tttCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATAC‑GGGtt < 1:2605100/64‑1 (MQ=255) tttCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATAC‑GGGgtt < 1:1998992/65‑1 (MQ=255) tttCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATAC‑GGGGTTCTTTCg > 1:862585/1‑71 (MQ=255) tCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATAC‑GGGGTTCTTTCg > 1:1459295/1‑69 (MQ=255) tCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATAC‑GGGGTTCTTTCGcc > 1:3495285/1‑71 (MQ=255) tCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATAC‑GGGGTTCTTTCGcc > 1:1439750/1‑71 (MQ=255) tCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATAC‑GGGGTTCTTTCGcc > 1:1932237/1‑71 (MQ=255) gTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATAC‑GGGTTTCTTTCGCCCg < 1:1048920/71‑1 (MQ=255) tCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATAC‑GGGGTTCTTTCGCCCgg < 1:1856485/71‑1 (MQ=255) gtccgcgcCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATAC‑GGGGTTCTTTCGCCCgg < 1:1890849/67‑1 (MQ=255) gcgcgcCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATAC‑GGGTTTCTTTCGCCCGGATg < 1:31466/71‑1 (MQ=255) gcgcgcCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATAC‑GGGGTTCTTTCGCCCGGATg < 1:1717987/71‑1 (MQ=255) gcgcCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATAC‑GGGGTTCTTTCGCCCGGATGaa < 1:1538851/71‑1 (MQ=255) cTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATAC‑GGGGTTCTTTCGCCCGGATGAAgcgc < 1:1147643/71‑1 (MQ=255) cTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATAC‑GGGGTTCTTTCGCCCGGATGAAgcgc < 1:2548551/71‑1 (MQ=255) tACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATAC‑GGGGTTCTTTCGCCCGGATGAAGCGCa > 1:3371329/1‑71 (MQ=255) cTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATAC‑GGGTTTCTTTCGCCCGGATGAAGCGCAc > 1:1245359/1‑70 (MQ=255) cTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATAC‑GGGGTTCTTTCGCCCGGATGAAGCGCAc > 1:2348935/1‑70 (MQ=255) ggCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATAC‑GGGGTTCTTTCGCCCGGATGAAGCGCACCa > 1:3470740/1‑70 (MQ=255) gCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATAC‑GGGGTTCTTTCGCCCGGATGAAGCGCACCAGc > 1:2276967/1‑71 (MQ=255) cATGAATGGCTTAACTGGCCGTCAATATTTGCCAATAC‑GGGGTTCTTTCGCCCGGATGAAGCGCACCAGc > 1:2225875/1‑70 (MQ=255) tGAATGGCTTAACTGGCCGTCAATATTTGCCAATAC‑GGGGTTCTTTCGCCCg < 1:449264/52‑1 (MQ=255) tGAATGGCTTAACTGGCCGTCAATATTTGCCAATAC‑GGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCg < 1:607657/70‑1 (MQ=255) tGAATGGCTTAACTGGCCGTCAATATTTGCCAATAC‑GGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGc < 1:3315597/71‑1 (MQ=255) gAATGGCTTAACTGGCCGTCAATATTTGCCAATAC‑GGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCa > 1:1117136/1‑71 (MQ=255) aaTGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCa > 1:4018728/1‑71 (MQ=255) aaTGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCa > 1:362734/1‑71 (MQ=255) aaTGGCTTAACTGGCCGTCAATATTTGCCAATAC‑GGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAt > 1:3829787/1‑71 (MQ=255) aaTGGCTTAACTGGCCGTCAATATTTGCCAATAC‑GGGTTTCTTTCCCCCGGATGAAGCGCACCAGCCGCAt > 1:4040513/1‑71 (MQ=255) aaTGGCTTAACTGGCCGTCAATATTTGCCAATAC‑GGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAt < 1:1817011/71‑1 (MQ=255) aaTGGCTTAACTGGCCGTCAATATTTGCCAATAC‑GGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAt > 1:2844895/1‑71 (MQ=255) aaTGGCTTAACTGGCCGTCAATATTTGCCAATAC‑GGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAt > 1:2195685/1‑71 (MQ=255) aaTGGCTTAACTGGCCGTCAATATTTGCCAATAC‑GGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAt > 1:808680/1‑71 (MQ=255) aaTGGCTTAACTGGCCGTCAATATTTGACAATAC‑GGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAt > 1:3069949/1‑71 (MQ=255) aTGGCTTAACTGGCCGTCAATATTTGCCAATAC‑GGGGTTCTTTCGCCCGGATGAAGCGCACCCGCCGCAtt > 1:3169330/1‑71 (MQ=255) tGGCTTAACTGGCCGTCAATATTTGCCAATAC‑GGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCa > 1:2231462/1‑68 (MQ=255) tGGCTTAACTGGCCGTCAATATTTGCCAATAC‑GGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAttt < 1:3731772/71‑1 (MQ=255) gCTTAACTGGCCGTCAATATTTGCCAATAC‑GGGGTTCTTTCGCCCGGATGAAGCGCAcc > 1:2669892/1‑59 (MQ=255) gCTTAACTGGCCGTCAATATTTGCCAATAC‑GGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAttt > 1:2300155/1‑69 (MQ=255) gCTTAACTGGCCGTCAATATTTGCCAATAC‑GGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAttt > 1:2097160/1‑69 (MQ=255) tAACTGGCCGTCAATATTTGCCAATAC‑GGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGc > 1:1186616/1‑70 (MQ=255) aaCTGGCCGTCAATATTTGCCAATAC‑GGGGTTCTTTCGccc > 1:3692798/1‑41 (MQ=255) aaCTGGCCGTCAATATTTGCCAATAC‑GGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGc < 1:796550/69‑1 (MQ=255) aCTGGCCGTCAATATTTGCCAATAC‑GGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGAc > 1:2823710/1‑71 (MQ=255) aCTGGCCGTCAATATTTGCCAATAC‑GGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGAc < 1:1961601/71‑1 (MQ=255) aCTGGCCGTCAATATTTGCCAATAC‑GGGGTTCTTTCGCCCGGATGAAGCGCACAAGCCGCATTTCAGCGAc > 1:3718078/1‑71 (MQ=255) ggCCGTCAATATTTGCCAATAC‑GGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTg > 1:372405/1‑71 (MQ=255) gCCGTCAATATTTGCCAATAC‑GGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCt < 1:3445189/69‑1 (MQ=255) gTCAATATTTGCCAATAC‑GGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTg < 1:3030171/71‑1 (MQ=255) tCAATATTTGCCAATAC‑GGGGTTCTTTCGCCCGGATGAAgcgc < 1:420807/43‑1 (MQ=255) tCAATATTTGCCAATAC‑GGGGTTCTTTCGCCCGGATGAAg < 1:3330301/40‑1 (MQ=255) aaTATTTGCCAATAC‑GGGGTTCTTTCGCCCGGATGaa > 1:3609107/1‑37 (MQ=255) aaTATTTGCCAATAC‑GGGGTTCTTTCGCCCGGATGaa > 1:618910/1‑37 (MQ=255) aaTATTTGCCAATAC‑GGGGTTCTTTCGCCCGGATGAAgcg > 1:2264983/1‑40 (MQ=255) aaTATTTGCCAATAC‑GGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAttt > 1:2061005/1‑54 (MQ=255) aaTATTTGCCAATAC‑GGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTggg > 1:3232849/1‑70 (MQ=255) aaTATTTGCCAATAC‑GGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTggg > 1:1903855/1‑70 (MQ=255) tatTTGCCAATAC‑GGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCaa < 1:1256724/71‑1 (MQ=255) tatTTGCCAATAC‑GGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCa > 1:2618030/1‑70 (MQ=255) tttGCCAATAC‑GGGGTTCTTTCGGCCGGATGAAGCGCACCAGCCGCa > 1:2240072/1‑47 (MQ=39) tttGCCAATAC‑GGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCaaa < 1:555665/70‑1 (MQ=255) tttGCCAATAC‑GGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCaaa > 1:119971/1‑70 (MQ=255) tttGCCAATAC‑GGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAAt > 1:652858/1‑71 (MQ=255) tttGCCAATAC‑GGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAAt < 1:909118/71‑1 (MQ=255) tttGCCAATAC‑GGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAAt > 1:3856786/1‑71 (MQ=255) ttGCCAATAC‑GGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAAt < 1:2919158/70‑1 (MQ=255) tGCCAATAC‑GGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATc < 1:3514068/70‑1 (MQ=255) gCCAATAC‑GGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCa < 1:783978/70‑1 (MQ=255) ccAATAC‑GGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCAtt < 1:1939613/71‑1 (MQ=255) ccAATAC‑GGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCAtt < 1:2763920/71‑1 (MQ=255) ccAATAC‑GGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCAtt < 1:384399/71‑1 (MQ=255) ccAATAC‑GGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCAtt < 1:2150976/71‑1 (MQ=255) cAATAC‑GGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCAtt < 1:2357677/70‑1 (MQ=255) aaTAC‑GGGGTTCTTTCGCCCGGATGAAGAGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCAtt < 1:2276167/69‑1 (MQ=255) aTAC‑GGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCAtt < 1:3070886/68‑1 (MQ=255) aTAC‑GGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCAtt < 1:689816/68‑1 (MQ=255) aTAC‑GGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCAtt < 1:1981224/68‑1 (MQ=255) tAC‑GGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACg > 1:1049335/1‑71 (MQ=255) tAC‑GGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACg > 1:1667562/1‑71 (MQ=255) tAC‑GGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACg > 1:2890336/1‑71 (MQ=255) tAC‑GGGGTTCTTTCGCCCGGATGAAGCGCA‑CAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGc > 1:1615942/1‑71 (MQ=255) tAC‑GGGGTTCA‑TCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGc < 1:138503/71‑1 (MQ=255) aC‑GGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCa > 1:3315110/1‑64 (MQ=255) aC‑GGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTaa > 1:2831390/1‑68 (MQ=255) aC‑GGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGc > 1:4035822/1‑71 (MQ=255) aC‑GGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGc > 1:59146/1‑71 (MQ=255) aC‑GGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGc > 1:703396/1‑71 (MQ=255) aC‑GGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGc > 1:3191525/1‑71 (MQ=255) c‑GGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGcc > 1:2675144/1‑71 (MQ=255) c‑GGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGcc > 1:2066235/1‑71 (MQ=255) c‑GGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGcc > 1:2690555/1‑71 (MQ=255) c‑GGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGcc > 1:3786618/1‑71 (MQ=255) tttctttcGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCAtt < 1:2417864/61‑1 (MQ=255) | GGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATAC‑GGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGCC > W3110S.gb/70671‑70806 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |