| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,225,032 | Δ1 bp | 100% | coding (143/1434 nt) | ygjI → | predicted transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,225,032 | 0 | T | . | 76.7% | 63.9 / 25.9 | 30 | coding (143/1434 nt) | ygjI | predicted transporter |
| Reads supporting (aligned to +/- strand): ref base T (7/0); new base . (9/14); total (16/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.31e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch. | |||||||||||
TCAATAATAATATTGAGCTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGATTTTTTATTTTATTCCCTTCTGTCTGATCATCGCAGAATTTGTTTCGTTAAATAAAA > W3110S.gb/3224975‑3225088 | tCAATAATAATATTGAGCTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGA‑TTTTTATTTTATTc < 1:1517994/71‑1 (MQ=255) taataataTTGAGCTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGA‑TTTTTATTTTATTCCCt > 1:3155946/1‑70 (MQ=255) aataataTTGAGCTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGA‑TTTTTATTTTATTCCCTtc > 1:466495/1‑71 (MQ=255) ataataTTGAGCTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGA‑TTTTTATTTTATTCCCTtc > 1:2364588/1‑70 (MQ=255) aataTTGAGCTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGATTttttatttta > 1:1467561/1‑61 (MQ=255) aataTTGAGCTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGATTttttatttta > 1:290221/1‑61 (MQ=255) aataTTGAGCTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGATTttttatttta > 1:2842250/1‑61 (MQ=255) aataTTGAGCTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGATTttttatttta > 1:3238173/1‑61 (MQ=255) aataTTGAGCTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGATTttttatttta > 1:707618/1‑61 (MQ=255) ttGAGCTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGAttt > 1:44722/1‑48 (MQ=255) gAGCTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGA‑TTTTTATTTTATTCCCTTCTGTCTGat > 1:2456490/1‑70 (MQ=255) gACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGA‑TTTTTATTTTATTCCCTTCTGTCTGATCATCGCAg < 1:3009981/71‑1 (MQ=255) gACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGA‑TTTTTATTTTATTCCCTTCTGTCTGATCATCGCAg < 1:3005692/71‑1 (MQ=255) gACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGA‑TTTTTATTTTATTCCCTTCTGTCTGATCATCGCAg < 1:3506964/71‑1 (MQ=255) gACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGA‑TTTTTATTTTATTCCCTTCTGTCTGATCATCGCAg < 1:3597755/71‑1 (MQ=255) cTGGCCTCGGCACCGATGTTTTTCCTCGCGACGA‑TTTTTATTTTATTCCCTTCTGTCTGATCATCGCAGaa > 1:208478/1‑71 (MQ=255) tGGCCTCGGCACCGATGTTTTTCCTCGCGACGA‑Tttttatttta < 1:330470/44‑1 (MQ=255) tGGCCTCGGCACCGATGTTTTTCCTCGCGACGA‑Tttttatttta < 1:1759760/44‑1 (MQ=255) tGGCCTCGGCACCGATGTTTTTCCTCGCGACGA‑Tttttatttta < 1:2850025/44‑1 (MQ=255) gCCTCGGCACCGATGTTTTTCCTCGCGACGA‑Tttttatttta < 1:1762879/42‑1 (MQ=255) tCGGCACCGATGTTTTTCCTCGCGACGA‑Tttttatttta < 1:808891/39‑1 (MQ=39) tCGGCACCGATGTTTTTCCTCGCGACGA‑Tttttatttta < 1:2673240/39‑1 (MQ=39) tCGGCACCGATGTTTTTCCTCGCGACGA‑Tttttatttta < 1:850935/39‑1 (MQ=39) tCGGCACCGATGTATTTCCTCGCGACGA‑Tttttatttta < 1:1474015/39‑1 (MQ=25) ggCACCGATGTTTTTCCTCGCGACGATTttttatt > 1:2959623/1‑35 (MQ=255) ggCACCGATGTTTTTCCTCGCGACGATTTtttattttat > 1:2812889/1‑39 (MQ=255) ggCACCGATGTTTTTCCTCGCGACGA‑TTTTTATTTTATTCCCTtc > 1:2348217/1‑45 (MQ=255) ccGATGTTTTTCCTCGCGACGA‑TTTTTATTTTATTCCCTTCTGTCTGATCATCGCAGAAttt < 1:239687/62‑1 (MQ=255) aTGTTTTTCCTCGCGACGA‑TTTTTATTTTATTCCCTTCTGTCTGATCATCGCAGAATTTGTTTCGTtaaat < 1:991063/71‑1 (MQ=255) tGTTTTTCCTCGCGACGA‑TTTTTATTTTATTCCCTTCTGTCTGATCATCGCAGAATTTGTTTCGTtaaata < 1:1108401/71‑1 (MQ=255) tGTTTTTCCTCGCGACGA‑TTTTTATTTTATTCCCTTCTGTCTGATCATCGCAGAATTTGTTTCGTtaaata < 1:1487203/71‑1 (MQ=255) tttttCCTCGCGACGA‑TTTTTATTTTATTCCCTtct < 1:2507771/36‑1 (MQ=38) tttttCCTCGCGACGA‑TTTTTATTTTATTCCCTtct < 1:2290109/36‑1 (MQ=38) tttttCCTCGCGACGA‑TTTTTATTTTATTCCCTtct < 1:2233139/36‑1 (MQ=38) tttttCCTCGCGACGA‑TTTTTATTTTATTCCCTTCTGTCTGATCATCGCAGAATTTGTTTCGTtaaataaa < 1:2197058/71‑1 (MQ=255) tttttCCTCGCGACGA‑TTTTTATTTTATTCCCTTCTGTCTGATCATCGCAGAATTTGTTTCGTtaaataaa < 1:2161410/71‑1 (MQ=255) tttttCCTCGCGACGA‑TTTTTATTTTATTCCCTTCTGTCTGATCATCGCAGAATTTGTTTCGTtaaataa > 1:3173200/1‑70 (MQ=255) tttttCCTCGCGACGA‑TTTTTATTTTATTCCCTTCTGTCTGATCATCGCAGAATTTGTTTCGTtaaataa > 1:2226798/1‑70 (MQ=255) tttCCTCGCGACGA‑TTTTTATTTTATTCCCTTCTGTCTGATCATCGCAGAATTTGTTTCGTTAAATaaaa > 1:3136126/1‑70 (MQ=255) | TCAATAATAATATTGAGCTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGATTTTTTATTTTATTCCCTTCTGTCTGATCATCGCAGAATTTGTTTCGTTAAATAAAA > W3110S.gb/3224975‑3225088 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |