| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,777,419:1 | +A | 100% | coding (3964/4581 nt) | ypjA ← | adhesin‑like autotransporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,777,419 | 1 | . | A | 88.1% | 107.3 / 11.9 | 42 | coding (3964/4581 nt) | ypjA | adhesin‑like autotransporter |
| Reads supporting (aligned to +/- strand): ref base . (2/3); new base A (14/23); total (16/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.02e-01 | |||||||||||
| Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch. | |||||||||||
CACTTTCATGTTCCCAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATC‑‑AAAACCGATATGTGAATGGGAATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATAT > W3110S.gb/2777350‑2777484 | cACTTTCATGTTCCCAACTGGCATAGCCGCCCAGAGAATGACTGCCCACACTGCCATGTCCTCCGCGATc > 1:2494885/1‑70 (MQ=255)cACTTTCATGTTCCCAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATc > 1:1467499/1‑70 (MQ=255) tCATGTTCCCAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGACATGTCCTCCGCGATC‑‑aaaaac > 1:1894528/1‑69 (MQ=255) aTGTTCCCAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCCATC‑‑aaaaacc < 1:322469/70‑4 (MQ=255) cAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCA‑AAAACCGATAtgtg < 1:2597504/71‑1 (MQ=255) cAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCA‑AAAACCGATAtgtg < 1:1624919/71‑1 (MQ=255) gCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCA‑AAAACCGATATGTGAATggg < 1:166099/71‑1 (MQ=255) gCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCA‑AAAACCGATATGTGAATggg < 1:1638561/71‑1 (MQ=255) gCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCA‑AAAACCGATATGTGAATgg < 1:2823866/70‑1 (MQ=255) gCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCA‑AAAACCGATATGTGAATgg < 1:1706529/70‑1 (MQ=255) gCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCA‑AAAACCGATATGTGAATgg < 1:1213691/70‑1 (MQ=255) gCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCA‑AAAACCGATATGTGAATgg < 1:83914/70‑1 (MQ=255) gccgccCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCA‑AAAACCGATATGTGAATGGGAATAg > 1:1577670/1‑71 (MQ=255) gccgccCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCA‑AAAACCGATATGTGAATGGGAATAg > 1:1653245/1‑71 (MQ=255) gccgccCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCA‑AAAACCGATATGTGAATGGGAATAg > 1:585641/1‑71 (MQ=255) gccgccCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATC‑‑AAAACCGATATGTGAATGGGAATAg > 1:2338051/1‑70 (MQ=255) ccgccCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATC‑‑AAAACCGATATGTGAATGGGAATAGcc < 1:363223/71‑1 (MQ=255) agagAATAACTGCCCACACTGCCATGTCCTCCGCGATCA‑AAAACCGATATGTGAATggg < 1:2482132/59‑1 (MQ=255) agagAATAACTGCCCACACTGCCATGTCCTCCGCGATCA‑AAAACCGATATGTGAATggg < 1:2430397/59‑1 (MQ=255) agagAATAACTGCCCACACTGCCATGTCCTCCGCGATCA‑AAAACCGATATGTGAATggg < 1:3432996/59‑1 (MQ=255) agagAATAACTGCCCACACTGCCATGTCCTCCGCGATCA‑AAAACCGATATGTGAATggg < 1:3666899/59‑1 (MQ=255) agagAATAACTGCCCACACTGCCATGTCCTCCGCGATCA‑AAAACCGATATGTGAATggg < 1:521574/59‑1 (MQ=255) gagaATAACTGCCCACACTGCCATGTCCTCCGCGATC‑‑aaaaa > 1:275616/1‑41 (MQ=255) gagaATAACTGCCCACACTGCCATGTCATCCGCGATCA‑AAAACCGATATGTGAATGGGAATAGCCCATaa > 1:2971531/1‑70 (MQ=255) agaATAACTGCCCACACTGCCATGTCCTCCGCGATCA‑AAAACCGATAtgtg < 1:2718460/51‑1 (MQ=255) gaATAACTGCCCACACTGCCATGTCCTCCGCGATCA‑AAAACCGATAtgtg < 1:2060866/50‑1 (MQ=255) aCTGCCCACACTGCCATGTCCTCCGCGATCA‑AAAACCGATATGTGAATGGGAATAGCCCATAAAAg > 1:3320128/1‑66 (MQ=255) aCTGCCCACACTGCCATGTCCTCCGCGATCA‑AAAACCGATATGTGAATGGGAATAGCCCATAAAAg > 1:1017638/1‑66 (MQ=255) aCTGCCCACACTGCCATGTCCTCCGCGATCA‑AAAACCGATATGTGAATGGGAATAGCCCATAAAAg > 1:1920622/1‑66 (MQ=255) aCTGCCCACACTGCCATGTCCTCCGCGATCA‑AAAACCGATATGTGAATGGGAATAGCCCATAAAAGCGcc > 1:2940566/1‑70 (MQ=255) acacTTCCATGTCCTCCGCGATC‑‑AAAACCGATATGTGAATGGGAATAGCCCATAAAAGCGCCTAGCGTGGt < 1:612617/71‑1 (MQ=255) acacTGCCATGTCCTCCGCGATC‑‑AAAACCGATATGTGAATGGGAATAGCCCATAAAAGCGCCTAGCGTGGt < 1:531338/71‑1 (MQ=255) cTGCCATGTCCTCCGCGATCA‑AAAACCGATATGTGAATGGGAATa > 1:1144199/1‑45 (MQ=255) cTGCCATGTCCTCCGCGATCA‑AAAACCGATATGTGAATGGGAATAGCCCATAAAAGCGCCTAGCGTGGTaa > 1:208177/1‑71 (MQ=255) cTGCCATGTCCTCCGCGATCA‑AAAACCGATATGTGAATGGGAATAGCCCATAAAAGCGCCTAGCGTGGTaa > 1:736545/1‑71 (MQ=255) ccATGTCCTCCGCGATCAAAAAACCGATATGTGAATGGGAATAGCCCATAAAAGCGCCTAGCGTGGTAAt < 1:2031746/70‑1 (MQ=255) ccATGTCCTCCGCGATCAAAAAACCGATATGTGAATGGGAATAGCCCATAAAAGCGCCTAGCGTGGTAAt < 1:446483/70‑1 (MQ=255) ccATGTCCTCCGCGATCA‑AAAACCGATATGTGAATggg < 1:183442/38‑1 (MQ=38) cATGTCCTCCGCGATC‑‑AAAACCGATATGTGAATGGGAATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCt > 1:3441373/1‑71 (MQ=255) tGTCCTCCGCGATCA‑AAAACCGATATGTGAATGGGAATAGCCCATAAAAGCGCCTAg < 1:842599/57‑1 (MQ=255) tGTCCTCCGCGATCA‑AAAACCGATATGTGAATGGGAATAGCCCATAAAAGCGCCTAg < 1:1238095/57‑1 (MQ=255) tcctccGCGATCA‑AAAACCGATATGTGAATGGGAATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCtt > 1:1002051/1‑69 (MQ=255) tcctccGCGATCA‑AAAACCGATATGTGAATGGGAATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCtt > 1:1148462/1‑69 (MQ=255) cgcgATCA‑AAAACCGATATGTGAATGGGAATAGCCCATAAAAg > 1:2268929/1‑43 (MQ=255) cgATCA‑AAAACCGATATGTGAATGGGAATAGCCCATAAAAGCGCCTAGCGTGGt < 1:3656260/54‑1 (MQ=39) cgATCA‑AAAACCGATATGTGAATGGGAATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAatat < 1:1129696/71‑1 (MQ=255) cgATCA‑AAAACCGATATGTGAATGGGAATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAatat < 1:2729006/71‑1 (MQ=255) | CACTTTCATGTTCCCAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATC‑‑AAAACCGATATGTGAATGGGAATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATAT > W3110S.gb/2777350‑2777484 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |