| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 619,623 | C→T | 33.5% | D310D (GAC→GAT) | rpsA → | 30S ribosomal subunit protein S1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 619,623 | 0 | C | T | 33.5% | 84.0 / 55.3 | 104 | D310D (GAC→GAT) | rpsA | 30S ribosomal subunit protein S1 |
| Reads supporting (aligned to +/- strand): ref base C (25/44); new base T (21/14); total (46/58) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.37e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01 | |||||||||||
Only 100 of 104 total aligned reads displayed.
ACGGCTGCTTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAAC‑AAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTAT > minE/619562‑619685 | aCGGCTGCTTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACca < 1:3106191/69‑1 (MQ=255)aCGGCTGCTTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACca < 1:1683428/69‑1 (MQ=255)aCGGCTGCTTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACca < 1:722298/69‑1 (MQ=255)aCGGCTGCTTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACca < 1:1734729/69‑1 (MQ=255)aCGGCTGCTTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACca < 1:1762622/69‑1 (MQ=255)aCGGCTGCTTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACca < 1:1220991/69‑1 (MQ=255)aCGGCTGCTTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACca < 1:3330946/69‑1 (MQ=255) tCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAAC‑AAAAACa < 1:791737/69‑1 (MQ=255) tCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAAC‑AAAAACa < 1:1920388/69‑1 (MQ=255) tCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAAC‑AAAAACa < 1:1355821/69‑1 (MQ=255) tCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAAC‑AAAAACa < 1:1287759/69‑1 (MQ=255) ttGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAAC‑AAAAACATc < 1:2364068/68‑1 (MQ=255) gaagaaGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAAC‑AAAAACATCCACCCGTc > 1:1569119/1‑68 (MQ=255) gaagaaGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAAC‑AAAAACATCCACCCGTc > 1:2913264/1‑68 (MQ=255) gaagaaGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAAC‑AAAAACATCCACCCGTc > 1:2832194/1‑68 (MQ=255) gaagaaGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAAC‑AAAAACATCCACCCGTc > 1:80842/1‑68 (MQ=255) agGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAAC‑AAAAACATCCACCCGTCCAAAgt < 1:1790287/69‑1 (MQ=255) agGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAAC‑AAAAACATCCACCCGTCCAAAgt < 1:2828783/69‑1 (MQ=255) agGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAAC‑AAAAACATCCACCCGTCCAAAgt < 1:2896119/69‑1 (MQ=255) agGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAAC‑AAAAACATCCACCCGTCCAAAgt < 1:1960879/69‑1 (MQ=255) agGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAAC‑AAAAACATCCACCCGTCCAAAgt < 1:2811421/69‑1 (MQ=255) agGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAAC‑AAAAACATCCACCCGTCCAAAgt < 1:2621213/69‑1 (MQ=255) agGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAAC‑AAAAACATCCACCCGTCCAAAgt < 1:1699691/69‑1 (MQ=255) agGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAAC‑AAAAACATCCACCCGTCCAAAgt < 1:1078085/69‑1 (MQ=255) agGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAAC‑AAAAACATCCACCCGTCCAAAgt < 1:3358301/69‑1 (MQ=255) ggCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAAC‑AAAAACATCCACCCGTCCAAAgtt < 1:381374/69‑1 (MQ=255) ggCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAAC‑AAAAACATCCACCCGTCCAAAgtt < 1:2733966/69‑1 (MQ=255) ggCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAAC‑AAAAACATCCACCCGTCCAAAgtt < 1:1047562/69‑1 (MQ=255) ggCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAAC‑AAAAACATCCACCCGTCCAAAgt < 1:2768416/68‑1 (MQ=255) gCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAAC‑AAAAACATCCACCCGTCCAAAgtt < 1:1489398/68‑1 (MQ=255) tGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAAC‑AAAAACATCCACCCGTCCAAAGTTGTTaa < 1:13755/69‑1 (MQ=255) tGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAAC‑AAAAACATCCACCCGTCCAAAGTTGTTaa < 1:201672/69‑1 (MQ=255) tGGTACACGTTTCCGAAATGGACTGGACCAACAAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGc < 1:175185/69‑1 (MQ=255) tACACGTTTCCGAAATGGACTGGACCAAC‑AAAAACATCCACCCGTcc > 1:691798/1‑47 (MQ=255) tACACGTTTCCGAAATGGACTGGACCAAC‑AAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGa > 1:3152822/1‑67 (MQ=255) tACACGTTTCCGAAATGGACTGGACCAAC‑AAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGa > 1:2497134/1‑67 (MQ=255) tACACGTTTCCGAAATGGACTGGACCAAC‑AAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGa > 1:175493/1‑67 (MQ=255) acacGTTTCCGAAATGGACTGGACCAAC‑AAAAACATCCACCCGTCCAAAGTTGTTAAc < 1:2446840/58‑1 (MQ=255) acacGTTTCCGAAATGGACTGGACCAAC‑AAAAACATCCACCCGTCCAAAGTTGTTAAc < 1:311987/58‑1 (MQ=255) acacGTTTCCGAAATGGACTGGACCAAC‑AAAAACATCCACCCGTCCAAAGTTGTTAAc < 1:2755882/58‑1 (MQ=255) acacGTTTCCGAAATGGACTGGACCAAC‑AAAAACATCCACCCGTCCAAAGTTGTTAAc < 1:86358/58‑1 (MQ=255) acacGTTTCCGAAATGGACTGGACCAAC‑AAAAACATCCACCCGTCCAAAGTTGTTAAc < 1:2042197/58‑1 (MQ=255) acacGTTTCCGAAATGGACTGGACCAAC‑AAAAACATCCACCCGTCCAAAGTTGTTAAc < 1:2015725/58‑1 (MQ=255) acacGTTTCCGAAATGGACTGGACCAAC‑AAAAACATCCACCCGTCCAAAGTTGTTAAc < 1:2895823/58‑1 (MQ=255) acacGTTTCCGAAATGGACTGGACCAAC‑AAAAACATCCACCCGTCCAAAGTTGTTAAc < 1:1276147/58‑1 (MQ=255) acacGTTTCCGAAATGGACTGGACCAAC‑AAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGAt < 1:3377888/67‑1 (MQ=255) acacGTTTCCGAAATGGACTGGACCAAC‑AAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGt < 1:3136531/69‑1 (MQ=255) acacGTTTCCGAAATGGACTGGACCAAC‑AAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGt < 1:891071/69‑1 (MQ=255) acacGTTTCCGAAATGGACTGGACCAAC‑AAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGt < 1:1006147/69‑1 (MQ=255) cacGTTTCCGAAATGGACTGGACCAAC‑AAAAACATCCACCCGTCCAAAGTTGTTAAc < 1:108249/57‑1 (MQ=255) acGTTTCCGAAATGGACTGGACCAAC‑AAAAACATCCACCCGTCCAAAGTTGTTAAc < 1:650437/56‑1 (MQ=255) gattCCGAAATGGATTGGACCAAC‑AAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGt > 1:2416216/3‑68 (MQ=255) gTTTCCGAAATGGATTGGACCAAC‑AAAAACATCCCCCCGTCCAAAGTTGTTAACGTTGGCGATGTAGt > 1:688381/1‑68 (MQ=255) gTTTCCGAAATGGATTGGACCAAC‑AAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAg > 1:1090313/1‑67 (MQ=255) gTTTCCGAAATGGATTGGACCAAC‑AAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAg > 1:336151/1‑67 (MQ=255) gTTTCCGAAATGGATTGGACCAAC‑AAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAg > 1:547139/1‑67 (MQ=255) gTTTCCGAAATGGATTGGACCAAC‑AAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGt > 1:605368/1‑68 (MQ=255) gTTTCCGAAATGGATTGGACCAAC‑AAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGt > 1:3311853/1‑68 (MQ=255) gTTTCCGAAATGGATTGGACCAAC‑AAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGt > 1:1425131/1‑68 (MQ=255) gTTTCCGAAATGGATTGGACCAAC‑AAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGt > 1:3410936/1‑68 (MQ=255) gTTTCCGAAATGGATTGGACCAAC‑AAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGt > 1:1494801/1‑68 (MQ=255) gTTTCCGAAATGGATTGGACCAAC‑AAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGt > 1:2983842/1‑68 (MQ=255) gTTTCCGAAATGGATTGGACCAAC‑AAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGt > 1:739392/1‑68 (MQ=255) gTTTCCGAAATGGATTGGACCAAC‑AAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGt > 1:2069752/1‑68 (MQ=255) gTTTCCGAAATGGATTGGACCAAC‑AAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGt > 1:1043807/1‑68 (MQ=255) gTTTCCGAAATGGATTGGACCAAC‑AAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGt > 1:708052/1‑68 (MQ=255) gTTTCCGAAATGGATTGGACCAAC‑AAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGt > 1:1909778/1‑68 (MQ=255) gTTTCCGAAATGGATTGGACCAAC‑AAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGt > 1:1914971/1‑68 (MQ=255) gTTTCCGAAATGGATTGGACCAAC‑AAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGt > 1:1047941/1‑68 (MQ=255) ttCCGAAATGGATTGGACCAAC‑AAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGa < 1:663287/69‑1 (MQ=255) ttCCGAAATGGATTGGACCAAC‑AAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGa < 1:1330020/69‑1 (MQ=255) ttCCGAAATGGATTGGACCAAC‑AAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGa < 1:824069/69‑1 (MQ=255) ttCCGAAATGGATTGGACCAAC‑AAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGa < 1:397431/69‑1 (MQ=255) ttCCGAAATGGATTGGACCAAC‑AAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGa < 1:841211/69‑1 (MQ=255) ttCCGAAATGGATTGGACCAAC‑AAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGa < 1:3248633/69‑1 (MQ=255) ttCCGAAATGGATTGGACCAAC‑AAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGa < 1:2210183/69‑1 (MQ=255) ttCCGAAATGGATTGGACCAAC‑AAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGa < 1:2766791/69‑1 (MQ=255) ttCCGAAATGGATTGGACCAAC‑AAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGa < 1:1995208/69‑1 (MQ=255) ttCCGAAATGGATTGGACCAAC‑AAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGa < 1:2847089/69‑1 (MQ=255) ttCCGAAATGGATTGGACCAAC‑AAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGa < 1:1714858/69‑1 (MQ=255) ttCCGAAATGGATTGGACCAAC‑AAAAACATCCACCCGTCCAAAGTTGTAAACGTTGGCGATGTAGTGGa < 1:2926389/69‑1 (MQ=255) ttCCGAAATGGATTGGACCAAC‑AAAAACATCCACACGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGa < 1:2107174/69‑1 (MQ=255) tCCGAAATGGACTGGACCAAC‑AAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGaa > 1:1000355/1‑69 (MQ=255) tCCGAAATGGACTGGACCAAC‑AAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGaa > 1:2324692/1‑69 (MQ=255) tCCGAAATGGACTGGACCAAC‑AAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGaa > 1:618827/1‑69 (MQ=255) tCCGAAATGGACTGGACCAAC‑AAAAACATCCACCCGTCCAAAGTTGTTAACGGTGGCGATGTAGTGGaa > 1:1756442/1‑69 (MQ=255) aTGGACTGGACCAAC‑AAAAACATCCACCCGTCCAAAGTTGTTAAc > 1:1159990/1‑45 (MQ=255) aTGGACTGGACCAAC‑AAAAACATCCACCCGTCCAAAGTTGTTAAc < 1:1221901/45‑1 (MQ=255) aTGGACTGGACCAAC‑AAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCg > 1:1887466/1‑52 (MQ=255) aTGGACTGGACCAAC‑AAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTAt > 1:1553103/1‑68 (MQ=255) aTGGACTGGACCAAC‑AAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTAt > 1:2402777/1‑68 (MQ=255) aTGGACTGGACCAAC‑AAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTAt > 1:2858082/1‑68 (MQ=255) aTGGACTGGACCAAC‑AAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTAt > 1:475575/1‑68 (MQ=255) aTGGACTGGACCAAC‑AAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTAt > 1:432902/1‑68 (MQ=255) aTGGACTGGACCAAC‑AAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTAt > 1:2041528/1‑68 (MQ=255) aTGGACTGGACCAAC‑AAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTAt > 1:75569/1‑68 (MQ=255) aTGGACTGGACCAAC‑AAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTAt > 1:3106572/1‑68 (MQ=255) aTGGACTGGACCAAC‑AAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTAt > 1:2045451/1‑68 (MQ=255) aTGGACTGGACCAAC‑AAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTAt > 1:1473362/1‑68 (MQ=255) aTGGACTGGACCAAC‑AAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTAt > 1:120995/1‑68 (MQ=255) | ACGGCTGCTTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAAC‑AAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTAT > minE/619562‑619685 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |