| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 621,170 | C→T | 41.0% | A51V (GCG→GTG) | ycaI → | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 621,170 | 0 | C | T | 41.0% | 41.3 / 66.3 | 95 | A51V (GCG→GTG) | ycaI | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (41/15); new base T (8/31); total (49/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.02e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.45e-01 | |||||||||||
TGCGTTTCTGACTCTCTTCGCCTGCGTACTGGCATTTATCCCTGTTAAAACCGTCCGTTATATCGCGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTATCAGCAAAGCAAATTTTGTGGGCAGGAGAAA > minE/621105‑621235 | tGCGTTTCTGACTCTCTTCGCCTGCGTACTGGCATTTATCCCTGTTAAAACCGTCCGTTATATCGCGCt > 1:2540422/1‑69 (MQ=255)tGCGTTTCTGACTCTCTTCGCCTGCGTACTGGCATTTATCCCTGTTAAAACCGTCCGTTATATCGCGCt > 1:2389319/1‑69 (MQ=255)tGCGTTTCTGACTCTCTTCGCCTGCGTACTGGCATTTATCCCTGTTAAAACCGTCCGTTATATCGCGCt > 1:1838668/1‑69 (MQ=255) tCTGACTCTCTTCTCCTGCGTACTGGCATTTATCCCTGTTAAAACCGTCCGTTATATCGCGCTGACGtt > 1:3022729/1‑69 (MQ=255) tCTGACTCTCTTCGCCTGCGTACTGGCATTTATCCCTGTTAAAACCGTCCGTTATATCGCGCTGACGtt > 1:3048533/1‑69 (MQ=255) tCTGACTCTCTTCGCCTGCGTACTGGCATTTATCCCTGTTAAAACCGTCCGTTATATCGCGCTGACGtt > 1:2662812/1‑69 (MQ=255) tCTGACTCTCTTCGCCTGCGTACTGGCATTTATCCCTGTTAAAACCGTCCGTTATATCGCGCTGACGtt < 1:2600189/69‑1 (MQ=255) tCTGACTCTCTTCGCCTGCGTACTGGCATTTATCCCTGTTAAAACCGTCCGTTATATCGCGCTGACGtt > 1:782987/1‑69 (MQ=255) tCTGACTCTCTTCGCCTGCGTACTGGCATTTATCCCTGTTAAAACCGTCCGTTATATCGCGCTGACGtt < 1:668677/69‑1 (MQ=255) tCTGACTCTCTTCGCCTGCGTACTGGCATTTATCCCTGTTAAAACCGTCCGTTATATCGCGCTGACGtt < 1:1982816/69‑1 (MQ=255) tCTGACTCTCTTCGCCTGCGTACTGGCATTTATCCCTGTTAAAACCGTCCGTTATATCGCGCTGACGtt > 1:1976398/1‑69 (MQ=255) tCTGACTCTCTTCGCCTGCGTACTGGCATTTATCCCTGTTAAAACCGTCCGTTATATCGCGCTGACGtt < 1:1951368/69‑1 (MQ=255) tCTGACTCTCTTCGCCTGCGTACTGGCATTTATCCCTGTTAAAACCGTCCGTTATATCGCGCTGACGtt > 1:916286/1‑69 (MQ=255) tCTGACTCTCTTCGCCTGCGTACTGGCATTTATCCCTGTTAAAACCGTCCGTTATATCGCGCTGACGtt < 1:544071/69‑1 (MQ=255) tCTGACTCTCTTCGCCTGCGTACTGGCATTTATCCCTGTTAAAACCGTCCGTTATATCGCGCTGACGtt > 1:1774373/1‑69 (MQ=255) tCTGACTCTCTTCGCCTGCGTACTGGCATTTATCCCTGTTAAAACCGTCCGTTATATCGCGCTGACGtt < 1:1099592/69‑1 (MQ=255) tCTGACTCTCTTCGCCTGCGTACTGGCATTTATCCCTGTTAAAACCGTCCGTTATATCGCGCTGACGtt < 1:1715458/69‑1 (MQ=255) tCTGACTCTCTTCGCCTGCGTACTGGCATTTATCCCTGTTAAAACCGTCCGTTATATCGCGCTGACGtt > 1:995989/1‑69 (MQ=255) ggCATTTATCCCTGTTAAAACCGTCCGTTATATCGCGCTGACGTTGCTGTTTTTCGTTTGGGGCAtatt < 1:317643/69‑1 (MQ=255) tATCCCTGTTAAAACCGTCCGTTATATCGCGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTATCag > 1:2496062/1‑68 (MQ=255) tATCCCTGTTAAAACCGTCCGTTATATCGCGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTATCag > 1:3123505/1‑68 (MQ=255) tATCCCTGTTAAAACCGTCCGTTATATCGCGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTATCag > 1:519209/1‑68 (MQ=255) tATCCCTGTTAAAACCGTCCGTTATATCGCGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTATCag > 1:3158563/1‑68 (MQ=255) tATCCCTGTTAAAACCGTCCGTTATATCGCGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTATCag > 1:3067077/1‑68 (MQ=255) tATCCCTGTTAAAACCGTCCGTTATATCGCGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTATCag > 1:927663/1‑68 (MQ=255) tATCCCTGTTAAAACCGTCCGTTATATCGCGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTATCag > 1:2757115/1‑68 (MQ=255) tATCCCTGTTAAAACCGTCCGTTATATCGCGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTATCag > 1:1416615/1‑68 (MQ=255) tATCCCTGTTAAAACCGTCCGTTATATCGCGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTATCa > 1:2845236/1‑67 (MQ=255) tATCCCTGTTAAAACCGTCCGTTATATCGCGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTATAag > 1:2425583/1‑68 (MQ=255) tATCCCTGTTAAAACCGTCCGTTATATCGCGCTGACGTTGCTCTTTTTCGTTTGGGGCATATTATCag > 1:313855/1‑68 (MQ=255) ccTGTTAAAACCGTCCGTTATATCGCGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTATCagcaaa > 1:801494/1‑68 (MQ=255) ccTGTTAAAACCGTCCGTTATATCGCGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTATCagcaaa > 1:2802747/1‑68 (MQ=255) ccTGTTAAAACCGTCCGTTATATCGCGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTATCagcaaa > 1:2908160/1‑68 (MQ=255) ccTGTTAAAACCGTCCGTTATATCGCGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTATCagcaaa > 1:2078721/1‑68 (MQ=255) ccTGTTAAAACCGTCCGTTATATCGCGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTATCagcaaa > 1:2963504/1‑68 (MQ=255) ccTGTTAAAACCGTCCGTTATATCGCGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTATCagcaaa > 1:158448/1‑68 (MQ=255) ccTGTTAAAACCGTCCGTTATATCGCGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTATCagcaaa > 1:1803480/1‑68 (MQ=255) ccTGTTAAAACCGTCCGTTATATCGCGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTATCagcaaa > 1:1666281/1‑68 (MQ=255) ccTGTTAAAACCGTCCGTTATATCGCGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTATCagcaa > 1:2843152/1‑67 (MQ=255) ttAAAACCGTCCGTTATATCGTGCTTACGTTGCTGTTTTTCGTTTGGGGCATATTAgcagca < 1:408283/62‑1 (MQ=255) ttAAAACCGTCCGTTATATCGTGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTAgcagca < 1:3218887/62‑1 (MQ=255) ttAAAACCGTCCGTTATATCGTGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTAgcagca < 1:3180989/62‑1 (MQ=255) ttAAAACCGTCCGTTATATCGTGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTAgcagca < 1:631276/62‑1 (MQ=255) ttAAAACCGTCCGTTATATCGTGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTAgcagca < 1:3254395/62‑1 (MQ=255) ttAAAACCGTCCGTTATATCGTGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTAgcagca < 1:37185/62‑1 (MQ=255) ttAAAACCGTCCGTTATATCGTGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTAgcagca < 1:3324679/62‑1 (MQ=255) ttAAAACCGTCCGTTATATCGTGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTAgcagca < 1:669172/62‑1 (MQ=255) ttAAAACCGTCCGTTATATCGTGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTAgcagca < 1:3417434/62‑1 (MQ=255) ttAAAACCGTCCGTTATATCGTGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTAgcagca < 1:795180/62‑1 (MQ=255) ttAAAACCGTCCGTTATATCGTGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTAgcagca < 1:1012951/62‑1 (MQ=255) ttAAAACCGTCCGTTATATCGTGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTAgcagca < 1:1126306/62‑1 (MQ=255) ttAAAACCGTCCGTTATATCGTGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTAgcagca < 1:997671/62‑1 (MQ=255) ttAAAACCGTCCGTTATATCGTGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTAgcagca < 1:1471838/62‑1 (MQ=255) ttAAAACCGTCCGTTATATCGTGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTAgcagca < 1:1495769/62‑1 (MQ=255) ttAAAACCGTCCGTTATATCGTGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTAgcagca < 1:95382/62‑1 (MQ=255) ttAAAACCGTCCGTTATATCGTGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTAgcagca < 1:1715845/62‑1 (MQ=255) ttAAAACCGTCCGTTATATCGTGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTAgcagca < 1:947785/62‑1 (MQ=255) ttAAAACCGTCCGTTATATCGTGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTAgcagca < 1:931501/62‑1 (MQ=255) ttAAAACCGTCCGTTATATCGTGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTAgcagca < 1:1862643/62‑1 (MQ=255) ttAAAACCGTCCGTTATATCGTGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTAgcagca < 1:1911870/62‑1 (MQ=255) ttAAAACCGTCCGTTATATCGTGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTAgcagca < 1:1946462/62‑1 (MQ=255) ttAAAACCGTCCGTTATATCGTGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTAgcagca < 1:826356/62‑1 (MQ=255) ttAAAACCGTCCGTTATATCGTGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTAgcagca < 1:2051552/62‑1 (MQ=255) ttAAAACCGTCCGTTATATCGTGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTAgcagca < 1:2139479/62‑1 (MQ=255) ttAAAACCGTCCGTTATATCGTGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTAgcagca < 1:2209974/62‑1 (MQ=255) ttAAAACCGTCCGTTATATCGTGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTAgcagca < 1:227752/62‑1 (MQ=255) ttAAAACCGTCCGTTATATCGTGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTAgcagca < 1:2359183/62‑1 (MQ=255) ttAAAACCGTCCGTTATATCGTGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTAgcagca < 1:2448296/62‑1 (MQ=255) tAAAACCGTCCGTTATATCGTGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTAgcagca < 1:1149840/61‑1 (MQ=255) tAAAACCGTCCGTTATATCGTGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTAgcagca < 1:1141688/61‑1 (MQ=255) aaaaCCGTCCGTTATATCGCGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTATCAGCAAAGCAAAtt > 1:899778/1‑69 (MQ=255) aaaaCCGTCCGTTATATCGCGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTATCAGCAAAGCAAAtt > 1:998316/1‑69 (MQ=255) aaaaCCGTCCGTTATATCGCGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTATCAGCAAAGCAAAtt > 1:1066897/1‑69 (MQ=255) aaaaCCGTCCGTTATATCGCGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTATCAGCAAAGCAAAtt > 1:1216772/1‑69 (MQ=255) aaaaCCGTCCGTTATATCGCGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTATCAGCAAAGCAAAtt > 1:337101/1‑69 (MQ=255) aaaaCCGTCCGTTATATCGCGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTATCAGCAAAGCAAAtt > 1:1805683/1‑69 (MQ=255) aaaaCCGTCCGTTATATCGCGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTATCAGCAAAGCAAAtt > 1:1824501/1‑69 (MQ=255) aaaaCCGTCCGTTATATCGCGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTATCAGCAAAGCAAAtt > 1:2489609/1‑69 (MQ=255) aaaaCCGTCCGTTATATCGCGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTATCAGCAAAGCAAAt > 1:394140/1‑68 (MQ=255) aaaaCCGTCCGTTATATCGCGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTATCAGCAAAGCAAAt > 1:2010576/1‑68 (MQ=255) ccgtTATATCGTGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTAGCagcaaagc > 1:1908807/1‑56 (MQ=255) ccgtTATATCGTGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTAGCAGCAAAGCAAATTTTGTGGGc > 1:2714586/1‑69 (MQ=255) ccgtTATATCGTGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTAGCAGCAAAGCAAATTTTGTGGGc > 1:2675930/1‑69 (MQ=255) ccgtTATATCGTGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTAGCAGCAAAGCAAATTTTGTGGGc > 1:2229861/1‑69 (MQ=255) ccgtTATATCGTGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTAGCAGCAAAGCAAATTTTGTGGGc > 1:1932597/1‑69 (MQ=255) ccgtTATATCGTGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTAGCAGCAAAGCAAATTTTGTGGGc > 1:1918785/1‑69 (MQ=255) ccgtTATATCGTGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTAGCAGCAAAGCAAATTTTGTGGGc > 1:335184/1‑69 (MQ=255) ccgtTATATCGTGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTAGCAGCAAAGCAAATTTTGTGGGc > 1:1285982/1‑69 (MQ=255) atCGCGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTATCAGCAAAGCAAATTTTGTGGGCAGgaga < 1:2720329/68‑1 (MQ=255) atCGCGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTATCAGCAAAGCAAATTTTGTGGGCAGgaga < 1:2426488/68‑1 (MQ=255) atCGCGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTATCAGCAAAGCAAATTTTGTGGGCAGgaga < 1:796984/68‑1 (MQ=255) atCGCGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTATCAGCAAAGCAAATTTTGTGGGCAGgaga < 1:553332/68‑1 (MQ=255) atCGCGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTATCAGCAAAGCAAATTTTGTGGGCAGgaga < 1:3104213/68‑1 (MQ=255) atCGCGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTATCAGCAAAGCAAATTTTGTGGGCAGgaga < 1:1225350/68‑1 (MQ=255) atCGCGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTATCAGCAAAGCAAATTTTGTGGGCAGgaga < 1:345790/68‑1 (MQ=255) cgcgCTGACGTTGCTGTTTTTCGTTTGGGGCATATTATCAGCAAAGCAAATTTTGTGGGCAGGAGaaa < 1:1513147/68‑1 (MQ=255) | TGCGTTTCTGACTCTCTTCGCCTGCGTACTGGCATTTATCCCTGTTAAAACCGTCCGTTATATCGCGCTGACGTTGCTGTTTTTCGTTTGGGGCATATTATCAGCAAAGCAAATTTTGTGGGCAGGAGAAA > minE/621105‑621235 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |