| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 676,582 | G→A | 43.0% | G57G (GGC→GGT) | ompA ← | outer membrane protein A (3a;II*;G;d) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 676,582 | 0 | G | A | 43.0% | 27.5 / 63.4 | 86 | G57G (GGC→GGT) | ompA | outer membrane protein A (3a;II*;G;d) |
| Reads supporting (aligned to +/- strand): ref base G (14/35); new base A (15/22); total (29/57) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.60e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.80e-01 | |||||||||||
TAACCAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGG‑TTTTCATGGGTCGGGCCATTGTTGTTGATGAAACCAGTGTCATGGTACTGGGACCAG > minE/676516‑676648 | tAACCAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAgcg > 1:3340944/1‑67 (MQ=255) cAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCTGCACCCAg > 1:2034601/1‑68 (MQ=255) cAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCTGCACCCAGt > 1:749057/1‑69 (MQ=255) cAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCTGCACCCAGt > 1:1769811/1‑69 (MQ=255) cAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCTGCACCCAGt > 1:220052/1‑69 (MQ=255) cAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCTGCACCCAGt > 1:1790136/1‑69 (MQ=255) cAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCTGCACCCAGt > 1:809132/1‑69 (MQ=255) cAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCTGCACCCAGt > 1:3231461/1‑69 (MQ=255) cAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCTGCACCCAGt > 1:1403015/1‑69 (MQ=255) cAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCTGCACCCAGt > 1:1310969/1‑69 (MQ=255) cAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCTGCACCCAGt > 1:1302960/1‑69 (MQ=255) cAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCTGCACCCAGt > 1:2932948/1‑69 (MQ=255) cAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCTGCACCCAGt > 1:2841507/1‑69 (MQ=255) cAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCTGCACCCAGt > 1:2907620/1‑69 (MQ=255) cAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCTGCACCCAGt > 1:1100410/1‑69 (MQ=255) cAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCTGCACCCAGt > 1:2896776/1‑69 (MQ=255) cGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGG‑t > 1:2035877/1‑69 (MQ=255) cGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGG‑t > 1:846972/1‑69 (MQ=255) aaCCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGG‑tttt < 1:1921744/69‑1 (MQ=255) aaCCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGG‑tttt < 1:1851496/69‑1 (MQ=255) aaCCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGG‑tttt < 1:1679099/69‑1 (MQ=255) aaCCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGG‑tttt < 1:79619/69‑1 (MQ=255) aCCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGG‑tttt < 1:2094238/68‑1 (MQ=255) aCCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGG‑tttt < 1:1908065/68‑1 (MQ=255) aCCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGG‑TTTTc > 1:3236695/1‑69 (MQ=255) ccATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCTGCACCCAGTTGG‑TTTTcgt < 1:3013130/69‑3 (MQ=255) ccATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCTGCACCCAGTTGG‑TTTTcgt < 1:2117824/69‑3 (MQ=255) ccATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCTGCACCCAGTTGG‑TTTTcgt < 1:3005944/69‑3 (MQ=255) ccATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCTGCACCCAGTTGG‑TTTTcgt < 1:3048548/69‑3 (MQ=255) ccATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCTGCACCCAGTTGG‑TTTTcgt < 1:765378/69‑3 (MQ=255) ccATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCTGCACCCAGTTGG‑TTTTcgt < 1:1888692/69‑3 (MQ=255) ccATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCTGCACCCAGTTGG‑TTTTcgt < 1:1899187/69‑3 (MQ=255) ccATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCTGCACCCAGTTGG‑TTTTcgt < 1:1154126/69‑3 (MQ=255) ccATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCTGCACCCAGTTGG‑TTTTcgt < 1:698466/69‑3 (MQ=255) ccATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCTGCACCCAGTTGG‑TTTTcgt < 1:1827175/69‑3 (MQ=255) ccATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCTGCACCCAGTTGG‑TTTTcgt < 1:69934/69‑3 (MQ=255) ccATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCTGCACCCAGTTGG‑TTTTcgt < 1:1719681/69‑3 (MQ=255) ccATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCTGCACCCAGTTGG‑TTTTcgt < 1:1387688/69‑3 (MQ=255) ccATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCTGCACCCAGTTGG‑TTTTcgt < 1:1395701/69‑3 (MQ=255) cATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCTGCACCCAGTTGG‑TTTTcgt < 1:1690918/68‑3 (MQ=255) cATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCTGCACCCAGTTGG‑TTTTcgt < 1:1165146/68‑3 (MQ=255) cATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCTGCACCCAGTTGG‑TTTTcgt < 1:3360547/68‑3 (MQ=255) cATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCTGCACCCAGTTGG‑TTTTcgt < 1:3061051/68‑3 (MQ=255) cATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCTGCACCCAGTTGG‑TTTTcgt < 1:1866287/68‑3 (MQ=255) cATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCTGCACCCAGTTGG‑TTTTcgt < 1:3065040/68‑3 (MQ=255) tCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGG‑TTTTCATGGGTc > 1:1487613/1‑69 (MQ=255) tCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGG‑TTTTCATGGGTc > 1:2313360/1‑69 (MQ=255) tCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGG‑TTTTCATGGGTc > 1:303273/1‑69 (MQ=255) tCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGG‑TTTTCATGGGTc > 1:383865/1‑69 (MQ=255) tCAAAGACAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGG‑TTTTCATGGGTc > 1:4395/1‑69 (MQ=255) cGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGG‑TTTTCATGGGTCGGGCCAttgttgtt < 1:1032542/69‑1 (MQ=255) cGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGG‑TTTTCATGGGTCGGGCCAttgttgtt > 1:515383/1‑69 (MQ=255) cGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGG‑TTTTCATGGGTCGGGCCAttgttgtt > 1:952881/1‑69 (MQ=255) cGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGG‑TTTTCATGGGTCGGGCCAttgttgtt > 1:1397097/1‑69 (MQ=255) cGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGG‑TTTTCATGGGTCGGGCCAttgttgtt < 1:2996575/69‑1 (MQ=255) cGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGG‑TTTTCATGGGTCGGGCCAttgttgtt < 1:3192789/69‑1 (MQ=255) cGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGG‑TTTTCATGGGTCGGGCCAttgttgtt > 1:3116482/1‑69 (MQ=255) cGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGG‑TTTTCATGGGTCGGGCCAttgttgtt < 1:1898340/69‑1 (MQ=255) cGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGG‑TTTTCATAGGTCGGGCCAttgttgtt < 1:2507194/69‑1 (MQ=255) tAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGG‑TTTTCATGGGTCGGGCCATTGTTGTtgatga < 1:2862663/69‑1 (MQ=255) tAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGG‑TTTTCATGGGTCGGGCCATTGTTGTtgatga < 1:1793834/69‑1 (MQ=255) tAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGG‑TTTTCATGGGTCGGGCCATTGTTGTtgatga < 1:2003673/69‑1 (MQ=255) aCCTGGTAACCACCAAAAGCACCTGCACCCAGTTGG‑TTTTcgt < 1:2916479/43‑3 (MQ=255) ccTGGTAACCACCAAAAGCACCTGCACCCAGTTGG‑TTTTcgt < 1:2563118/42‑3 (MQ=255) cTGGTAACCACCAAAAGCACCAGCGCCCAGTTGG‑TTTTCATGGGTCGGGCCATTGTTGTTGATGAAAcc < 1:3087038/69‑1 (MQ=255) cTGGTAACCACCAAAAGCACCAGCGCCCAGTTGG‑TTTTCATGGGTCGGGCCATTGTTGTTGATGAAAcc < 1:2866599/69‑1 (MQ=255) cTGGTAACCACCAAAAGCACCAGCGCCCAGTTGG‑TTTTCATGGGTCGGGCCATTGTTGTTGATGAAAcc < 1:103237/69‑1 (MQ=255) cTGGTAACCACCAAAAGCACCAGCGCCCAGTTGG‑TTTTCATGGGTCGGGCCATTGTTGTTGATGAAAcc < 1:116053/69‑1 (MQ=255) cTGGTAACCACCAAAAGCACCAGCGCCCAGTTGG‑TTTTCATGGGGCGGGCCATTGTTGTTGATGAAAcc < 1:1207649/69‑1 (MQ=255) tGGTAACCACCAAAAGCACCAGCGCCCAGTTGGTTTTTCATGGGTCGGGCCATTGTTGTTGATGAAAcc > 1:2213659/1‑69 (MQ=255) tGGTAACCACCAAAAGCA‑CAGCGCCCAGTTGG‑TTTTCATGGGTCGGGCCATTGTTGTTGATGAAACCAg < 1:1904658/69‑1 (MQ=255) ggTAACCACCAAAAGCACCAGCGCCCAGTTGG‑TTTTCATGGGTCGGGCCATTGTTGTTGATGAAACCAg < 1:3268724/69‑1 (MQ=255) ggTAACCACCAAAAGCACCAGCGCCCAGTTGG‑TTTTCATGGGTCGGGCCATTGTTGTTGATGAAACCAg < 1:3433079/69‑1 (MQ=255) ggTAACCACCAAAAGCACCAGCGCCCAGTTGG‑TTTTCATGGGTCGGGCCATTGTTGTTGATGAAACCAg < 1:1407387/69‑1 (MQ=255) ggTAACCACCAAAAGCACCAGCGCCCAGTTGG‑TTTTCATGGGTCGGGCCATTGTTGTTGATGAAACCAg < 1:3222272/69‑1 (MQ=255) ggTAACCACCAAAAGCACCAGCGCCCAGTTGG‑TTTTCATGGGTCGGGCCATTGTTGTTGATGAAACCAg < 1:2145526/69‑1 (MQ=255) ggTAACCACCAAAAGCACCAGCGCCCAGTTGG‑TTTTCATGGGTCGGGACATTGTTGTTGATGAAACCAg < 1:372351/69‑1 (MQ=255) ggTAACCACCAAAAGCACCAGCGCCCAGTTGG‑TTTTCATGGGGCGGGCCATTGTTGTTGATGAAACCAg < 1:2513864/69‑1 (MQ=255) ggTAACCACCAAAAGCACCAGCGCCCAGTTGG‑TTTTCATGGGGCGGGCCATTGTTGTTGATGAAACCAg < 1:1929973/69‑1 (MQ=255) aaCCACCAAAAGCACCAGCGCCCAGTTGG‑TTTTCATGGGTCGGGCCATTGTTGTTGATGAAACTAgtgt < 1:2483159/69‑1 (MQ=255) aaCCACCAAAAGCACCAGCGCCCAGTTGG‑TTTTCATGGGTCGGGCCATTGTTGTTGATGAAACCAgtgt < 1:2102136/69‑1 (MQ=255) caccaAAAGCACCAGCGCCCAGTTGG‑TTTTCATGGGTCGGGCCATTGTTGTTGATGAAACCAGTGTCa > 1:2936598/1‑68 (MQ=255) caccaAAAGCACCAGCGCCCAGTTGG‑TTTTCATGGGTCGGGCCATTGTTGTTGATGAAACCAGTGTCAt < 1:117931/69‑1 (MQ=255) caccaAAAGCACCAGCGCCCAGTTGG‑TTTTCATGGGTCGGGCCATTGTTGTTGATGAAACCAGTGTCAt < 1:975944/69‑1 (MQ=255) ccaAAAGCACCAGCGCCCAGTTGG‑TTTTCATGGGTCGGGCCATTGTTGTTGATGAAACCAGTGTCATgg < 1:3431460/69‑1 (MQ=255) ccaAAAGCACCAGCGCCCAGTTGG‑TTTTCATGGGTCGGGCCATTGTTGTTGATGAAACCAGTGTCATgg < 1:3389133/69‑1 (MQ=255) ccaAAAGCACCAGCGCCCAGTTGG‑TTTTCATGGGTCGGGCCATTGTTGTTGATGAAACCAGTGTCATgg < 1:3161152/69‑1 (MQ=255) gcCCAGTTGG‑TTTTCATGGGTCGGGCCATTGTTGTTGATGAAACCAGTGTCATGGTACTGGGACCAg > 1:2702033/1‑67 (MQ=255) | TAACCAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGG‑TTTTCATGGGTCGGGCCATTGTTGTTGATGAAACCAGTGTCATGGTACTGGGACCAG > minE/676516‑676648 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |