| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,347,113 | A→G | 26.8% | A202A (GCA→GCG) | yegH → | fused predicted membrane proteins |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,347,113 | 0 | A | G | 26.8% | 70.8 / 25.3 | 67 | A202A (GCA→GCG) | yegH | fused predicted membrane proteins |
| Reads supporting (aligned to +/- strand): ref base A (38/11); new base G (4/14); total (42/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.52e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GATCGTTATTCTCTGCTTAAGCTTCCTGTTGATGATTGGCTTTAGCCTGGTGGCAGAAGGTTTCGGCTTCGTCATTCCGAAAGGCTACCTGTACGCTGCCATTGGTTTCTCGGTGATG > minE/1347059‑1347176 | gaTCGTTATTCTCTGCTTAAGCTTCCTGTTGATGATTGGCTTTAGCCTGGTGGCAGAAGGTTTCGGCtt > 1:2008452/1‑69 (MQ=255) aTCGTTATTCTCTTCTTAAGCTTCCTGTTGATGATTGGCTTTAGCCTGGTGGCGGAAGGTTTCGGCTTc < 1:3393353/69‑1 (MQ=255) aTCGTTATTCTCTGCTTAAGCTTCCTGTTGATGATTGGCTTTAGCCTGGTGGCGGAAGGTTTCGGCTTc < 1:2935949/69‑1 (MQ=255) aTCGTTATTCTCTGCTTAAGCTTCCTGTTGATGATTGGCTTTAGCCTGGTGGCGGAAGGTTTCGGCTTc < 1:1502533/69‑1 (MQ=255) aTCGTTATTCTCTGCTTAAGCTTCCTGTTGATGATTGGCTTTAGCCTGGTGGCGGAAGGTTTCGGCTTc < 1:1548664/69‑1 (MQ=255) aTCGTTATTCTCTGCTTAAGCTTCCTGTTGATGATTGGCTTTAGCCTGGTGGCGGAAGGTTTCGGCTTc < 1:3311159/69‑1 (MQ=255) aTCGTTATTCTCTGCTTAAGCTTCCTGTTGATGATTGGCTTTAGCCTGGTGGCGGAAGGTTTCGGCTTc < 1:3361496/69‑1 (MQ=255) aTCGTTATTCTCTGCTTAAGCTTCCTGTTGATGATTGGCTTTAGCCTGGTGGCGGAAGGTTTCGGCTTc < 1:2450596/69‑1 (MQ=255) aTCGTTATTCTCTGCTTAAGCTTCCTGTTGATGATTGGCTTTAGCCTGGTGGCGGAAGGTTTCGGCTTc < 1:2443149/69‑1 (MQ=255) aTCGTTATTCTCTGCTTAAGCTTCCTGTTGATGATTGGCTTTAGCCTGGTGGCGGAAGGTTTCGGCTTc < 1:1963192/69‑1 (MQ=255) aTCGTTATTCTCTGCTTAAGCTTCCTGTTGATGATTGGCTTTAGCCTGGTGGCGGAAGGTTTCGGCTTc < 1:1241989/69‑1 (MQ=255) aTCGTTATTCTCTGCTTAAGCTTCCTGTTGATGATTGGCTTTAGCCTGGTGGCGGAAGGTTTCGGCTTc < 1:753634/69‑1 (MQ=255) cGTTATTCTCTGCTTAAGCTTCCTGTTGATGATTGGCTTTAGCCTGGTGGCAGAAGGt > 1:1974820/1‑58 (MQ=255) cGTTATTCTCTGCTTAAGCTTCCTGTTGATGATTGGCTTTAGCCTGGTGGCAGAAGGt > 1:2809764/1‑58 (MQ=255) cGTTATTCTCTGCTTAAGCTTCCTGTTGATGATTGGCTTTAGCCTGGTGGCAGAAGGt > 1:2091903/1‑58 (MQ=255) cGTTATTCTCTGCTTAAGCTTCCTGTTGATGATTGGCTTTAGCCTGGTGGCAGAAGGt > 1:2315473/1‑58 (MQ=255) cGTTATTCTCTGCTTAAGCTTCCTGTTGATGATTGGCTTTAGCCTGGTGGCAGAAGGt > 1:3131553/1‑58 (MQ=255) aTTCTCTGCTTAAGCTTCCTGTTGATGATTGGCTTTAGCCTGGTGGCAGAAGGt > 1:3384616/1‑54 (MQ=255) aTTCTCTGCTTAAGCTTCCTGTTGATGATTGGCTTTAGCCTGGTGGCAGAAGGt > 1:651517/1‑54 (MQ=255) aTTCTCTGCTTAAGCTTCCTGTTGATGATTGGCTTTAGCCTGGTGGCAGAAGGt > 1:1193088/1‑54 (MQ=255) aTTCTCTGCTTAAGCTTCCTGTTGATGATTGGCTTTAGCCTGGTGGCAGAAGGt > 1:1998525/1‑54 (MQ=255) aTTCTCTGCTTAAGCTTCCTGTTGATGATTGGCTTTAGCCTGGTGGCAGAAGGTTTCGGCTTCGt < 1:3216625/65‑1 (MQ=255) aTTCTCTGCTTAAGCTTCCTGTTGATGATTGGCTTTAGCCTGGTGGCAGAAGGTTTCGGCTTCGt < 1:1970479/65‑1 (MQ=255) tGCTTAAGCTTCCTGTTGATGATTGGCTTTAGCCTGGTGGCGGAAGGTTTCGGCTTc < 1:1385201/57‑1 (MQ=255) tAAGCTTCCTGTTGATGATTGGCTTTAGCCTGGTGGCAGAAgg > 1:697213/1‑43 (MQ=255) tAAGCTTCCTGTTGATGATTGGCTTTAGCCTGGTGGCAGAAgg > 1:1103642/1‑43 (MQ=255) tAAGCTTCCTGTTGATGATTGGCTTTAGCCTGGTGGCAGAAgg > 1:1137503/1‑43 (MQ=255) tAAGCTTCCTGTTGATGATTGGCTTTAGCCTGGTGGCAGAAGGTTTCGGCTTCGTCATTCCGAAAGGCt < 1:91591/69‑1 (MQ=255) tAAGCTTCCTGTTGATGATTGGCTTTAGCCTGGTGGCAGAAGGTTTCGGCTTCGTCATTCCGAAAGGCt < 1:2484366/69‑1 (MQ=255) cTTCCTGTTGATGATTGGCTTTAGCCTGGTGGCGGATGGTTTCGGCTTc < 1:862081/49‑1 (MQ=38) gatgatTGGCTTTAGCCTGGTGGCAGAAGGTTTCGGc > 1:2216821/1‑37 (MQ=255) gatgatTGGCTTTAGCCTGGTGGCAGAAGGTTTCGGc > 1:1184326/1‑37 (MQ=255) gatgatTGGCTTTAGCCTGGTGGCAGAAGGTTTCGGc > 1:1185000/1‑37 (MQ=255) gatgatTGGCTTTAGCCTGGTGGCAGAAGGTTTCGGc > 1:700481/1‑37 (MQ=255) gatgatTGGCTTTAGCCTGGTGGCAGAAGGTTTCGGc > 1:1365317/1‑37 (MQ=255) gatgatTGGCTTTAGCCTGGTGGCAGAAGGTTTCGGc > 1:143648/1‑37 (MQ=255) gatgatTGGCTTTAGCCTGGTGGCAGAAGGTTTCGGc > 1:2919405/1‑37 (MQ=255) gatgatTGGCTTTAGCCTGGTGGCAGAAGGTTTCGGc > 1:2900572/1‑37 (MQ=255) gatgatTGGCTTTAGCCTGGTGGCAGAAGGTTTCGGc > 1:1776763/1‑37 (MQ=255) gatgatTGGCTTTAGCCTGGTGGCAGAAGGTTTCGGc > 1:2333464/1‑37 (MQ=255) gatgatTGGCTTTAGCCTGGTGGCAGAAGGTTTCGGc > 1:2613604/1‑37 (MQ=255) gatgatTGGCTTTAGCCTGGTGGCAGAAGGTTTCGGc > 1:2373319/1‑37 (MQ=255) gatgatTGGCTTTAGCCTGGTGGCAGAAGGTTTCGGc > 1:188615/1‑37 (MQ=255) gatgatTGGCTTTAGCCTGGTGGCAGAAGGTTTCAGc > 1:1252202/1‑37 (MQ=255) atgatTGGCTTTAGCCTGGTGGCGGAAGGTTTCGGCTTc < 1:3015723/39‑1 (MQ=255) tAGCCTGGTGGCGGAAGGTTTCGGCTTCGTCATTCCGAAAGGCTACCTGTACGCTGCCATTGGTTtctc > 1:888658/1‑69 (MQ=255) tAGCCTGGTGGCGGAAGGTTTCGGCTTCGTCATTCCGAAAGGCTACCTGTACGCTGCCATTGGTTtctc > 1:633052/1‑69 (MQ=255) tAGCCTGGTGGCGGAAGGTTTCGGCTTCGTCATTCCGAAAGGCTACCTGTACGCTGCCATTGGTTtctc > 1:1487984/1‑69 (MQ=255) tAGCCTGGTGGCGGAAGGTTTCGGCTTCGTCATTCCGAAAGGCTACCTGTACGCTGCCATTGGTTtctc > 1:335759/1‑69 (MQ=255) gCCTGGTGGCAGAAGGTTTCGGCTTCGTCATTCCGAAAGGCTACCTGTACGCTGCCATTGGTTTCTCg > 1:2663809/1‑68 (MQ=255) gCCTGGTGGCAGAAGGTTTCGGCTTCGTCATTCCGAAAGGCTACCTGTACGCTGCCATTGGTTTCTCg > 1:404474/1‑68 (MQ=255) ccTGGTGGCAGAAGGTTTCGGCTTCGTCATTCCGCAAGGCTACCTGTACGCTGCCATTGGTTTCTCg > 1:316678/1‑67 (MQ=255) ccTGGTGGCAGAAGGTTTCGGCTTCGTCATTCCGAAAGGc > 1:2099097/1‑40 (MQ=255) ccTGGTGGCAGAAGGTTTCGGCTTCGTCATTCCGAAAGGCTACCTGTACGCTGCCATTGGTTTCTCg > 1:1272550/1‑67 (MQ=255) ccTGGTGGCAGAAGGTTTCGGCTTCGTCATTCCGAAAGGCTACCTGTACGCTGCCATTGGTTTCTCg > 1:3165279/1‑67 (MQ=255) ccTGGTGGCAGAAGGTTTCGGCTTCGTCATTCCGAAAGGCTACCTGTACGCTGCCATTGGTTTCTCg > 1:460891/1‑67 (MQ=255) ccTGGTGGCAGAAGGTTTCGGCTTCGTCATTCCGAAAGGCTACCTGTACGCTGCCATTGGTTTCTCg > 1:1511120/1‑67 (MQ=255) ccTGGTGGCAGAAGGTTTCGGCTTCGTCATTCCGAAAGGCTACCTGTACGCTGCCATTGGTTTCTCg > 1:2752307/1‑67 (MQ=255) ccTGGTGGCAGAAGGTTTCGGCTTCGTCATTCCGAAAGGCTACCTGTACGCTGCCATTGGTTTCTCg > 1:2615893/1‑67 (MQ=255) catggtggCAGAAGGTTTCGGCTTCGTCATTCCGAAAGGCTACCTGt > 1:1188763/3‑47 (MQ=255) ggCAGAAGGTTTCGGCTTCGTCATTCCGAAAGGCTACCTGTACGCTGCCATTGGTTTCTCGGtgatg < 1:3285552/67‑1 (MQ=255) ggCAGAAGGTTTCGGCTTCGTCATTCCGAAAGGCTACCTGTACGCTGCCATTGGTTTCTCGGtgatg < 1:1452437/67‑1 (MQ=255) ggCAGAAGGTTTCGGCTTCGTCATTCCGAAAGGCTACCTGTACGCTGCCATTGGTTTCTCGGtgatg < 1:1481876/67‑1 (MQ=255) ggCAGAAGGTTTCGGCTTCGTCATTCCGAAAGGCTACCTGTACGCTGCCATTGGTTTCTCGGtgatg < 1:1877373/67‑1 (MQ=255) ggCAGAAGGTTTCGGCTTCGTCATTCCGAAAGGCTACCTGTACGCTGCCATTGGTTTCTCGGtgatg < 1:2516721/67‑1 (MQ=255) ggCAGAAGGTTTCGGCTTCGTCATTCCGAAAGGCTACCTGTACGCTGCCATTGGTTTCTCGGtgatg < 1:2096721/67‑1 (MQ=255) gCAGAAGGTTTCGGCTTCGTCATTCCGAAAGGCTACCTGTACGCTGCCATTGGTTTCTCGGtgatg < 1:1913711/66‑1 (MQ=255) | GATCGTTATTCTCTGCTTAAGCTTCCTGTTGATGATTGGCTTTAGCCTGGTGGCAGAAGGTTTCGGCTTCGTCATTCCGAAAGGCTACCTGTACGCTGCCATTGGTTTCTCGGTGATG > minE/1347059‑1347176 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |