| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,756,442 | C→T | 45.1% | L344L (CTA→TTA) | pssA → | phosphatidylserine synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,756,442 | 0 | C | T | 45.1% | 47.2 / 153.0 | 186 | L344L (CTA→TTA) | pssA | phosphatidylserine synthase |
| Reads supporting (aligned to +/- strand): ref base C (38/64); new base T (28/56); total (66/120) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.45e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01 | |||||||||||
Only 100 of 189 total aligned reads displayed.
TTATCTCTATGAGATCAATCTGCGTCGTTTCCTGAGCCGTTTGCAGTATTACGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGATGTGGGTTGATGATA > minE/1756375‑1756508 | ttATCTCTATGAGATCAATCTGCGTCGTTTCCTGAGCCGTTTGCAGTATTACGTCAATACTGACCAGc < 1:446337/68‑1 (MQ=255) gagaTCAATCTGCGTCGTTTCCTGAGTCGTTTACAGTATTACGTCAACACTGACCAGTTAGTAGTTct < 1:939776/68‑2 (MQ=255) gagaTCAATCTGCGTCGTTTCCTGAGTCGTTTACAGTATTACGTCAACACTGACCAGTTAGTAGTTCg < 1:2368325/68‑1 (MQ=255) gagaTCAATCTGCGTCGTTTCCTGAGTCGTTTACAGTATTACGTCAACACTGACCAGTTAGTAGTTCg < 1:2129218/68‑1 (MQ=255) gagaTCAATCTGCGTCGTTTCCTGAGTCGTTTACAGTATTACGTCAACACTGACCAGTTAGTAGTTCg < 1:2619505/68‑1 (MQ=255) gagaTCAATCTGCGTCGTTTCCTGAGTCGTTTACAGTATTACGTCAACACTGACCAGTTAGTAGTTCg < 1:3073973/68‑1 (MQ=255) gagaTCAATCTGCGTCGTTTCCTGAGTCGTTTACAGTATTACGTCAACACTGACCAGTTAGTAGTTCg < 1:2149545/68‑1 (MQ=255) gagaTCAATCTGCGTCGTTTCCTGAGTCGTTTACAGTATTACGTCAACACTGACCAGTTAGTAGTTCg < 1:1507944/68‑1 (MQ=255) gagaTCAATCTGCGTCGTTTCCTGAGTCGTTTACAGTATTACGTCAACACTGACCAGTTAGTAGTTCg < 1:429627/68‑1 (MQ=255) gagaTCAATCTGCGTCGTTTCCTGAGTCGTTTACAGTATTACGTCAACACTGACCAGTTAGTAGTTCg < 1:423666/68‑1 (MQ=255) agaTCAATCTGCGTCGTTTCCTGAGTCGTTTACAGTATTACGTCAACACTGACCAGTTAGTAGTTCg < 1:1558661/67‑1 (MQ=255) cTGCGTCGTTTCCTGAGCCGTTTGCAGTATTACGTCAATACTGACCAGCTAGTGGTTCGGTTATGGaaa < 1:2682802/69‑1 (MQ=255) tGCGTCGTTTCCTGAGTCGTTTACAGTATTACGTCAACACTGACCAGTTAGTAGTTCg < 1:1399586/58‑1 (MQ=255) tGCGTCGTTTCCTGAGCCGTTTGCAGTATTACGTCAATACTGACCAGCTAGTGGTTCGGTTATGGaaa < 1:2357163/68‑1 (MQ=255) gtTTCCTGAGCCGTTTGCAGTATTACGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATgac > 1:918884/1‑68 (MQ=255) tCCTGAGCCGTTTGCAGTATTACGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATgacgac > 1:1179319/1‑68 (MQ=255) gAGCCGTTTGCAGTATTACGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGacaaca < 1:3355795/68‑1 (MQ=255) gAGCCGTTTGCAGTATTACGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGacaaca < 1:3336335/68‑1 (MQ=255) gAGCCGTTTGCAGTATTACGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGacaaca < 1:1249782/68‑1 (MQ=255) gAGCCGTTTGCAGTATTACGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGacaaca < 1:589929/68‑1 (MQ=255) gAGCCGTTTGCAGTATTACGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGacaaca < 1:2202632/68‑1 (MQ=255) gAGCCGTTTGCAGTATTACGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGacaaca < 1:1749154/68‑1 (MQ=255) gAGCCGTTTGCAGTATTACGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGacaaca < 1:1809533/68‑1 (MQ=255) gTTTGCAGTATTACGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATc < 1:2818523/69‑1 (MQ=255) gTTTGCAGTATTACGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATc < 1:1800224/69‑1 (MQ=255) tacAGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGACAACACCt < 1:199133/61‑1 (MQ=255) tacAGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGACAACACCt < 1:1958922/61‑1 (MQ=255) tacAGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGACAACACCt < 1:2736018/61‑1 (MQ=255) tacAGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGACAACACCt < 1:2282048/61‑1 (MQ=255) tacAGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGACAACACCt < 1:2938663/61‑1 (MQ=255) tacAGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGACAACACCt < 1:3010465/61‑1 (MQ=255) tacAGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGACAACACCt < 1:3250307/61‑1 (MQ=255) tacAGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGACAACACCt < 1:64711/61‑1 (MQ=255) tacAGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGACAACACCt < 1:1011682/61‑1 (MQ=255) tGCAGTATTACGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCAc > 1:2248073/1‑68 (MQ=255) cAGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGACAACACCTATCACCt < 1:2504962/68‑1 (MQ=255) cAGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGACAACACCTATCACCt < 1:2328344/68‑1 (MQ=255) cAGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGACAACACCTATCACCt < 1:2747249/68‑1 (MQ=255) cAGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGACAACACCTATCACCt < 1:2276683/68‑1 (MQ=255) cAGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGACAACACCTATCACCt < 1:2951638/68‑1 (MQ=255) cAGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGACAACACCTATCACCt < 1:608397/68‑1 (MQ=255) cAGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGACAACACCTATCACCt < 1:685084/68‑1 (MQ=255) cAGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGACAACACCTATCACCt < 1:1042082/68‑1 (MQ=255) cAGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGACAACACCTATCACCt < 1:1147587/68‑1 (MQ=255) cAGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGACAACACCTATCACCt < 1:144967/68‑1 (MQ=255) aGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATgac > 1:2799500/1‑50 (MQ=255) aGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGacaa > 1:1595799/1‑55 (MQ=255) aGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGacaa > 1:94965/1‑55 (MQ=255) aGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGacaa > 1:1130465/1‑55 (MQ=255) aGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGacaa > 1:1274363/1‑55 (MQ=255) aGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGacaa > 1:2986943/1‑55 (MQ=255) aGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGacaa > 1:2916145/1‑55 (MQ=255) aGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGacaa > 1:1805079/1‑55 (MQ=255) aGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGacaa > 1:2712524/1‑55 (MQ=255) aGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGacaa > 1:1869901/1‑55 (MQ=255) aGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGacaa > 1:26503/1‑55 (MQ=255) aGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGacaa > 1:2262929/1‑55 (MQ=255) aGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGacaa > 1:2079713/1‑55 (MQ=255) aGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGacaa > 1:2386005/1‑55 (MQ=255) aGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGacaa > 1:2350388/1‑55 (MQ=255) aGTATTACGACAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGacaa > 1:2856131/1‑55 (MQ=255) ttACGTCAATACTGACCAGCTATTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGATAgg < 1:41267/69‑1 (MQ=255) ttACGTCAATACTGACCAGCTATTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAgg < 1:1189713/69‑1 (MQ=255) ttACGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAgg < 1:2345761/69‑1 (MQ=255) ttACGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAgg > 1:452965/1‑69 (MQ=255) ttACGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAgg < 1:3235699/69‑1 (MQ=255) ttACGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAgg < 1:2790983/69‑1 (MQ=255) tACGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAgg < 1:3092827/68‑1 (MQ=255) aCGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGa < 1:932620/69‑1 (MQ=255) aCGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGa > 1:1568515/1‑69 (MQ=255) aCGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGa < 1:147300/69‑1 (MQ=255) aCGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGa < 1:1359223/69‑1 (MQ=255) gTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGACAACACCTATCACCt < 1:1657480/59‑1 (MQ=255) cAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGAtgtg < 1:1897055/69‑1 (MQ=255) cAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGAtgtg < 1:767440/69‑1 (MQ=255) cAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGAtgtg < 1:1928534/69‑1 (MQ=255) aaTACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGATGTgg < 1:1735889/69‑1 (MQ=255) aaTACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGATGTgg < 1:1714798/69‑1 (MQ=255) aaTACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGATGTgg < 1:1251077/69‑1 (MQ=255) aaTACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGATGTgg < 1:385948/69‑1 (MQ=255) aaTACTGACCAGCTAGTGGTCCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGATGTgg < 1:203566/69‑1 (MQ=255) aTACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGAtgtg < 1:1967003/67‑1 (MQ=255) aTACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGATGTgg < 1:1827047/68‑1 (MQ=255) aCTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGAt > 1:2054592/1‑62 (MQ=255) aCTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGATGTGGGtt > 1:2597088/1‑69 (MQ=255) aCTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGATGTGGGtt > 1:2213937/1‑69 (MQ=255) aCTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGATGTGGGtt > 1:3186670/1‑69 (MQ=255) aCTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGATGTGGGtt > 1:120816/1‑69 (MQ=255) aCTGACCAGCTAGTGGTTCGGTTATAGAAAGATGACGACAACACCTATCACCTGAAAGGGATGTGGGtt > 1:833125/1‑69 (MQ=255) gACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGATGTgg < 1:549653/63‑1 (MQ=255) aCCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGATGTGGGTtgat > 1:627064/1‑68 (MQ=255) aCCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGATGTGGGTtgat > 1:1085996/1‑68 (MQ=255) aCCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGATGTGGGTtgat > 1:2106364/1‑68 (MQ=255) aCCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGATGTGGGTtgat > 1:2576016/1‑68 (MQ=255) aCCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGATGTGGGTtgat > 1:631186/1‑68 (MQ=255) aCCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGATGTGGGTtgat > 1:2556185/1‑68 (MQ=255) aCCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGATGTGGGTtgat > 1:418497/1‑68 (MQ=255) aCCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGCGATGTGGGTtga > 1:1239901/1‑67 (MQ=255) aGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGATGTGGGTTGATGATa < 1:3153520/69‑1 (MQ=255) cTAGTGGTTCGGTTATGTAAAGATGACGACAACACCTATCACCTGAAAGGGa < 1:1073745/52‑1 (MQ=255) | TTATCTCTATGAGATCAATCTGCGTCGTTTCCTGAGCCGTTTGCAGTATTACGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGATGTGGGTTGATGATA > minE/1756375‑1756508 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |