| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,963,098 | A→G | 31.3% | L140L (TTG→CTG) | yggC ← | conserved hypothetical protein with nucleoside triphosphate hydrolase domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,963,098 | 0 | A | G | 31.3% | 119.3 / 71.1 | 137 | L140L (TTG→CTG) | yggC | conserved hypothetical protein with nucleoside triphosphate hydrolase domain |
| Reads supporting (aligned to +/- strand): ref base A (49/45); new base G (15/28); total (64/73) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.75e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.78e-01 | |||||||||||
Only 100 of 137 total aligned reads displayed.
GCCACTTTTCATCATCCAGTAACAACCAGTTTCCTTCGACGATGACGAGTGGTGCGGTAACGTGCAACGCATCTTCAACAGGATCATGCTTTTGTCGATCGTACTGCGGCCACGTACAATCCCCTTCCACG > minE/1963032‑1963162 | gCCACTTTTCATCATCCAGTAACAACCAGTTTCCTTCGACGATGACGAGTGGTGCGGTAACGTGCAACg < 1:1016849/69‑1 (MQ=255)gCCACTTTTCATCATCCAGTAACAACCAGTTTCCTTCGACGATGACGAGTGGTGCGGTAACGTGCAACg < 1:2249512/69‑1 (MQ=255)gCCACTTTTCATCATCCAGTAACAACCAGTTTCCTTCGACGATGACGAGTGGTGCGGTAACGTGCAACg < 1:1981012/69‑1 (MQ=255)gCCACTTTTCATCATCCAGTAACAACCAGTTTCCTTCGACGATGACGAGTGGTGCGGTAACGTGCAACg < 1:3349182/69‑1 (MQ=255)gCCACTTTTCATCATCCAGTAACAACCAGTTTCCTTCGACGATGACGAGTGGTGCGGTAACGTGCAACg < 1:476044/69‑1 (MQ=255)gCCACTTTTCATCATCCAGTAACAACCAGTTTCCTTCGACGATGACGAGTGGTGCGGTAACGTGCAACg < 1:662543/69‑1 (MQ=255)gCCACTTTTCATCATCCAGTAACAACCAGTTTCCTTCGACGATGACGAGTGGTGCGGTAACCTGCAACg < 1:3069515/69‑1 (MQ=255) ccACTTTTCATCATCCAGTAACAACCAGTTTCCTTCGACGATGACGAGTGGTGCGGTAACGTGCAACg < 1:745411/68‑1 (MQ=255) tttCATCATCCAGTAACAACCAGTTTCCTTCGACGATGACGAGTGGTGCGGTAACGTGCAACGCATCt > 1:1271201/1‑68 (MQ=255) tttCATCATCCAGTAACAACCAGTTTCCTTCGACGATGACGAGTGGTGCGGTAACGTGCAACGCATCt > 1:205977/1‑68 (MQ=255) tttCATCATCCAGTAACAACCAGTTTCCTTCGACGATGACGAGTGGTGCGGTAACGTGCAACGCATCt > 1:2520599/1‑68 (MQ=255) tttCATCATCCAGTAACAACCAGTTTCCTTCGACGATGACGAGTGGTGCGGTAACGTGCAACGCATCt > 1:1448810/1‑68 (MQ=255) tttCATCATCCAGTAACAACCAGTTTCCTTCGACGATGACGAGTGGTGCGGTAACGTGCAACGCATCt > 1:3382968/1‑68 (MQ=255) ttCATCATCCAGTAACAACCAGTTTCCTTCGACGATGACGAGTGGTGCGGTAACGTGCAACGCATCTTc > 1:2160406/1‑69 (MQ=255) ttCATCATCCAGTAACAACCAGTTTCCTTCGACGATGACGAGTGGTGCGGTAACGTGCAACGCATCTTc > 1:1434840/1‑69 (MQ=255) acaacCAGTTTCCTTCGACTATGACGAGTGGTGCGGTAACGTGCAACGCATCTTCAACAGGATCATGCt < 1:3235443/69‑1 (MQ=255) acaacCAGTTTCCTTCGACGATGACGAGTGGTGCTGTAACGTGCAACGCATCTTCAACAGGATCATGCt < 1:2602152/69‑1 (MQ=255) acaacCAGTTTCCTTCGACGATGACGAGTGGTGCGGTAACGTGCAACGCATCTTCAACAGGATCATGc < 1:1382670/68‑1 (MQ=255) acaacCAGTTTCCTTCGACGATGACGAGTGGTGCGGTAACGTGCAACGCATCTTCAACAGGATCATGCt > 1:1990794/1‑69 (MQ=255) acaacCAGTTTCCTTCGACGATGACGAGTGGTGCGGTAACGTGCAACGCATCTTCAACAGGATCATGCt < 1:3157376/69‑1 (MQ=255) acaacCAGTTTCCTTCGACGATGACGAGTGGTGCGGTAACGTGCAACGCATCTTCAACAGGATCATGCt < 1:3038987/69‑1 (MQ=255) acaacCAGTTTCCTTCGACGATGACGAGTGGTGCGGTAACGTGCAACGCATCTTCAACAGGATCATGCt > 1:869538/1‑69 (MQ=255) acaacCAGTTTCCTTCGACGATGACGAGTGGTGCGGTAACGTGCAACGCATCTTCAACAGGATCATGCt > 1:3241197/1‑69 (MQ=255) acaacCAGTTTCCTTCGACGATGACGAGTGGTGCGGTAACGTGCAACGCATCTTCAACAGGATCATGCt > 1:2425464/1‑69 (MQ=255) acaacCAGTTTCCTTCGACGATGACGAGTGGTGCGGTAACGTGCAACGCATCTTCAACAGGATCATGCt < 1:3418146/69‑1 (MQ=255) acaacCAGTTTCCTTCGACGATGACGAGTGGTGCGGTAACGTGCAACGCATCTTCAACAGGATCATGCt < 1:1143543/69‑1 (MQ=255) acaacCAGTTTCCTTCGACGATGACGAGTGGTGCGGTAACGTGCAACGCATCTTCAACAGGATCATGCt < 1:1220757/69‑1 (MQ=255) acaacCAGTTTCCTTCGACGATGACGAGTGGTGCGGTAACGTGCAACGCATCTTCAACAGGATCATGCt > 1:483531/1‑69 (MQ=255) acaacCAGTTTCCTTCGACGATGACGAGTGGTGCGGTAACGTGCAACGCATCTTCAACAGGATCATGCt > 1:1418983/1‑69 (MQ=255) acaacCAGTTTCCTTCGACGATGACGAGTGGTGCGGTAACGTGCAACGCATCTTCAACAGGATCATGCt < 1:42016/69‑1 (MQ=255) acaacCAGTTTCCTTCGACGATGACGAGTGGTGCGGTAACGTGCAACGCATCTTCAACAGGATCATGCt > 1:693536/1‑69 (MQ=255) acaacCAGTTTCCTTCGACGATGACGAGTGGTGCGGTAACGTGCAACGCATCTTCAACAGGATCATGCt < 1:1581334/69‑1 (MQ=255) acaacCAGTTTCCTTCGACGATGACGAGTGGTGCGGTAACGTGCAACGCATCTTCAACAGGATCATGCt < 1:1582671/69‑1 (MQ=255) acaacCAGTTTCCTTCGACGATGACGAGTGGTGCGGTAACGTGCAACGCATCTTCAACAGGATCATGCt > 1:1619668/1‑69 (MQ=255) acaacCAGTTTCCTTCGACGATGACGAGTGGTGCGGTAACGTGCAACGCATCTTCAACAGGATCATGCt < 1:3263231/69‑1 (MQ=255) acaacCAGTTTCCTTCGACGATGACGAGTGGTGCGGTAACGTGCAACGCATCTTCAACAGCATCATGCt < 1:2496483/69‑1 (MQ=255) caacCAGTTTCCTTCGACGATGACGAGTGGTGCGGTAACGTGCAACGCATCTTCAACAGGATCATGCt < 1:2784107/68‑1 (MQ=255) acCAGTTTCCTTCGACGATGACGAGTGGTTCGGTAACGTGCAACGCATCTTCAACAGGATCATGCtttt < 1:1113727/69‑1 (MQ=255) acCAGTTTCCTTCGACGATGACGAGTGGTGCGGTAACGTGCAACGCATCTTCAACAGGATCATGCtttt < 1:2919492/69‑1 (MQ=255) acCAGTTTCCTTCGACGATGACGAGTGGTGCGGTAACGTGCAACGCATCTTCAACAGGATCATGCtttt < 1:2937314/69‑1 (MQ=255) acCAGTTTCCTTCGACGATGACGAGTGGTGCGGTAACGTGCAACGCATCTTCAACAGGATCATGCtttt < 1:3001647/69‑1 (MQ=255) acCAGTTTCCTTCGACGATGACGAGTGGTGCGGTAACGTGCAACGCATCTTCAACAGGATCATGCtttt < 1:2649017/69‑1 (MQ=255) acCAGTTTCCTTCGACGATGACGAGTGGTGCGGTAACGTGCAACGCATCTTCAACAGGATCATGCtttt < 1:2282382/69‑1 (MQ=255) acCAGTTTCCTTCGACGATGACGAGTGGTGCGGTAACGTGCAACGCATCTTCAACAGGATCATGCtttt < 1:1675985/69‑1 (MQ=255) ccAGTTTCCTTCGACGATGACGAGTGGTGCGGTAACGTGCAACGCATCTTCAACAGGATCATGCtttt < 1:3013082/68‑1 (MQ=255) ccAGTTTCCTTCGACGATGACGAGTGGTGCGGTAACGTGCAACGCATCTTCAACAGGATCATGCtttt < 1:49176/68‑1 (MQ=255) ccAGTTTCCTTCGACGATGACGAGTGGTGCGGTAACGTGCAACGCATCTTCAACAGGATCATGCtttt < 1:1974173/68‑1 (MQ=255) ttCCTTCGACGATGACGAGTGGTGCGGTAACGTGCAACGCATCTTCAACAGGATCATGCTTTTGTCGAt > 1:2919457/1‑69 (MQ=255) cGAGTGGTGCGGTAACGTGCAGCGCATCTTCAACAGGATCATGCTTTTGTCGATCGTACTGCg < 1:1289906/63‑1 (MQ=255) cGAGTGGTGCGGTAACGTGCAGCGCATCTTCAACAGGATCATGCTTTTGTCGATCGTACTGCg < 1:918259/63‑1 (MQ=255) cGAGTGGTGCGGTAACGTGCAGCGCATCTTCAACAGGATCATGCTTTTGTCGATCGTACTGCg < 1:1033023/63‑1 (MQ=255) cGAGTGGTGCGGTAACGTGCAGCGCATCTTCAACAGGATCATGCTTTTGTCGATCGTACTGCg < 1:3361155/63‑1 (MQ=255) cGAGTGGTGCGGTAACGTGCAGCGCATCTTCAACAGGATCATGCTTTTGTCGATCGTACTGCg < 1:3192211/63‑1 (MQ=255) cGAGTGGTGCGGTAACGTGCAGCGCATCTTCAACAGGATCATGCTTTTGTCGATCGTACTGCg < 1:3181037/63‑1 (MQ=255) cGAGTGGTGCGGTAACGTGCAGCGCATCTTCAACAGGATCATGCTTTTGTCGATCGTACTGCg < 1:3157654/63‑1 (MQ=255) cGAGTGGTGCGGTAACGTGCAGCGCATCTTCAACAGGATCATGCTTTTGTCGATCGTACTGCg < 1:1746356/63‑1 (MQ=255) cGAGTGGTGCGGTAACGTGCAGCGCATCTTCAACAGGATCATGCTTTTGTCGATCGTACTGCg < 1:156992/63‑1 (MQ=255) cGAGTGGTGCGGTAACGTGCAGCGCATCTTCAACAGGATCATGCTTTTGTCGATCGTACTGCg < 1:1748814/63‑1 (MQ=255) cGAGTGGTGCGGTAACGTGCAGCGCATCTTCAACAGGATCATGCTTTTGTCGATCGTACTGCg < 1:385446/63‑1 (MQ=255) cGAGTGGTGCGGTAACGTGCAGCGCATCTTCAACAGGATCATGCTTTTGTCGATCGTACTGCg < 1:833676/63‑1 (MQ=255) cGAGTGGTGCGGTAACGTGCAGCGCATCTTCAACAGGATCATGCTTTTGTCGATCGTACTGCg < 1:1171395/63‑1 (MQ=255) cGAGTGGTGCGGTAACGTGCAGCGCATCTTCAACAGGATCATGCTTTTGTCGATCGTACTGCg < 1:494980/63‑1 (MQ=255) cGAGTGGTGCGGTAACGTGCAGCGCATCTTCAACAGGATCATGCTTTTGTCGATCGTACTGCg < 1:2910120/63‑1 (MQ=255) cGAGTGGTGCGGTAACGTGCAGCGCATCTTCAACAGGATCATGCTTTTGTCGATCGTACTGCg < 1:514742/63‑1 (MQ=255) cGAGTGGTGCGGTAACGTGCAGCGCATCTTCAACAGGATCATGCTTTTGTCGATCGTACTGCg < 1:54984/63‑1 (MQ=255) cGAGTGGTGCGGTAACGTGCAGCGCATCTTCAACAGGATCATGCTTTTGTCGATCGTACTGCg < 1:717804/63‑1 (MQ=255) cGAGTGGTGCGGTAACGTGCAGCGCATCTTCAACAGGATCATGCTTTTGTCGATCGTACTGCg < 1:2772347/63‑1 (MQ=255) cGAGTGGTGCGGTAACGTGCAGCGCATCTTCAACAGGATCATGCTTTTGTCGATCGTACTGCg < 1:2704492/63‑1 (MQ=255) cGAGTGGTGCGGTAACGTGCAGCGCATCTTCAACAGGATCATGCTTTTGTCGATCGTACTGCg < 1:636137/63‑1 (MQ=255) cGAGTGGTGCGGTAACGTGCAGCGCATCTTCAACAGGATCATGCTTTTGTCGATCGTACTGCg < 1:264576/63‑1 (MQ=255) gCGGTAACGTGCAACTCATCTTCAACAGGATCATGCTTTTGTCGATCGTACTGCGGCCACGTACAATcc < 1:1098435/69‑1 (MQ=255) gCGGTAACGTGCAACGCATCTTCAACAGGATCATGCTTTTGTCGATCGTACTGTGGCCACGTACAATcc > 1:3256189/1‑69 (MQ=255) gCGGTAACGTGCAACGCATCTTCAACAGGATCATGCTTTTGTCGATCGTACTGCGGCCACGTACAATcc < 1:1596994/69‑1 (MQ=255) gCGGTAACGTGCAACGCATCTTCAACAGGATCATGCTTTTGTCGATCGTACTGCGGCCACGTACAATcc < 1:3097033/69‑1 (MQ=255) gCGGTAACGTGCAACGCATCTTCAACAGGATCATGCTTTTGTCGATCGTACTGCGGCCACGTACAATcc < 1:799040/69‑1 (MQ=255) gCGGTAACGTGCAACGCATCTTCAACAGGATCATGCTTTTGTCGATCGTACTGCGGCCACGTACAATcc > 1:1312112/1‑69 (MQ=255) gCGGTAACGTGCAACGCATCTTCAACAGGATCATGCTTTTGTCGATCGTACTGCGGCCACGTACAATcc > 1:1876444/1‑69 (MQ=255) gCGGTAACGTGCAACGCATCTTCAACAGGATCATGCTTTTGTCGATCGTACTGCGGCCACGTACAATcc > 1:1014703/1‑69 (MQ=255) gCGGTAACGTGCAACGCATCTTCAACAGGATCATGCTTTTGTCGATCGTACTGCGGCCACGTACAATcc < 1:562747/69‑1 (MQ=255) cGGTAACGTGCAGCGCATCTTCAACAGGATCATGCTTTTGTCGATCGTACTGCGGCCACGTACAATccc > 1:1726014/1‑69 (MQ=255) cGGTAACGTGCAGCGCATCTTCAACAGGATCATGCTTTTGTCGATCGTACTGCGGCCACGTACAATccc > 1:2494709/1‑69 (MQ=255) cGGTAACGTGCAGCGCATCTTCAACAGGATCATGCTTTTGTCGATCGTACTGCGGCCACGTACAATccc > 1:2039164/1‑69 (MQ=255) cGGTAACGTGCAGCGCATCTTCAACAGGATCATGCTTTTGTCGATCGTACTGCGGCCACGTACAATccc > 1:2570631/1‑69 (MQ=255) cGGTAACGTGCAGCGCATCTTCAACAGGATCATGCTTTTGTCGATCGTACTGCGGCCACGTACAATccc > 1:389427/1‑69 (MQ=255) cGGTAACGTGCAGCGCATCTTCAACAGGATCATGCTTTTGTCGATCGTACTGCGGCCACGTACAATccc > 1:2011438/1‑69 (MQ=255) cGGTAACGTGCAGCGCATCTTCAACAGGATCATGCTTTTGTCGATCGTACTGCGGCCACGTACAATccc > 1:1838456/1‑69 (MQ=255) cGGTAACGTGCAGCGCATCTTCAACAGGATCATGCTTTTGTCGATCGTACTGCGGCCACGTACAATccc > 1:1211943/1‑69 (MQ=255) cGGTAACGTGCAGCGCATCTTAAACAGGATCATGCTTTTGTCGATCGTACTGCGGCCACGTACAATccc > 1:2431748/1‑69 (MQ=255) gTAACGTGCAACGCATCTTCAACAGGATCATGCTTTTGTCGATCGTACTGCGGCCACGTACAATCCCCt > 1:2847921/1‑69 (MQ=255) gTAACGTGCAACGCATCTTCAACAGGATCATGCTTTTGTCGATCGTACTGCGGCCACGTACAATCCCCt > 1:1972888/1‑69 (MQ=255) gTAACGTGCAACGCATCTTCAACAGGATCATGCTTTTGTCGATCGTACTGCGGCCACGTACAATCCCCt > 1:1825609/1‑69 (MQ=255) gTAACGTGCAACGCATCTTCAACAGGATCATGCTTTTGTCGATCGTACTGCGGCCACGTACAATCCCCt > 1:3431562/1‑69 (MQ=255) gTAACGTGCAACGCATCTTCAACAGGATCATGCTTTTGTCGATCGTACTGCGGCCACGTACAATCCCCt > 1:2179791/1‑69 (MQ=255) gTAACGTGCAACGCATCTTCAACAGGATCATGCTTTTGTCGATCGTACTGCGGCCACGTACAATCCCCt > 1:1513474/1‑69 (MQ=255) gTAACGTGCAACGCATCTTCAACAGGATCATGCTTTTGTCGATCGTACTGCGGCCACGTACAATCCCCt > 1:1675763/1‑69 (MQ=255) gTAACGTGCAACGCATCTTCAACAGGATCATGCTTTTGTCGATCGTACTGCGGCCACGTACAATCACCt > 1:1442470/1‑69 (MQ=255) tGCAACGCATCTTCAACAGGATCATGCTTTTGTCGATCGTACTGCGGCCACGTACAATCCCCTTCCACg > 1:540709/1‑69 (MQ=255) tGCAACGCATCTTCAACAGGATCATGCTTTTGTCGATCGTACTGCGGCCACGTACAATCCCCTTCCACg > 1:367868/1‑69 (MQ=255) tGCAACGCATCTTCAACAGGATCATGCTTTTGTCGATCGTACTGCGGCCACGTACAATCCCCTTCCACg > 1:796035/1‑69 (MQ=255) gCAGCGCATCTTCAACAGGATCATGCTTTTGTCGATCGTACTGCg < 1:2909117/45‑1 (MQ=255) | GCCACTTTTCATCATCCAGTAACAACCAGTTTCCTTCGACGATGACGAGTGGTGCGGTAACGTGCAACGCATCTTCAACAGGATCATGCTTTTGTCGATCGTACTGCGGCCACGTACAATCCCCTTCCACG > minE/1963032‑1963162 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |