| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,296,406 | A→G | 31.9% | G555G (GGT→GGC) | katG ← | catalase/hydroperoxidase HPI(I) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,296,406 | 0 | A | G | 31.9% | 59.3 / 37.7 | 75 | G555G (GGT→GGC) | katG | catalase/hydroperoxidase HPI(I) |
| Reads supporting (aligned to +/- strand): ref base A (24/27); new base G (10/14); total (34/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.04e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.48e-01 | |||||||||||
CCGTCAGCAATTGGCTCCAGCAGCTCAAACATCTCAATGTCAGTCTGATCCTGACGCGCATCAACGCGACCCGGCGCAAACGGTACATGAATGCTCAAACCTGCGGCGCTTGCGGCTTTCTCAACACCAACCACA > minE/2296338‑2296472 | ccGTCAGCAATTGGCTCCAGCAGCTCAAACATCTCAATGTCAGTCTGATCCTGACGCGCATCAACGCGa < 1:433191/69‑1 (MQ=255)ccGTCAGCAATTGGCTCCAGCAGCTCAAACATCTCAATGTCAGTCTGATCCTGACGCGCATCAACGCGa < 1:2447050/69‑1 (MQ=255)ccGTCAGCAATTGGCTCCAGCAGCTCAAACATCTCAATGTCAGTCTGATCCTGACGCGCATCAACGCGa < 1:149779/69‑1 (MQ=255)ccGTCAGCAATTGGCTCCAGCAGCTCAAACATCTCAATGTCAGTCTGATCCTGACGCGCATCAACGCGa < 1:2728784/69‑1 (MQ=255) cAATTGGCTCCAGCAGCTCAAACATCTCAATGTCAGTCTGATCCTGACGCGCATCAACGCGACCCGgc > 1:2158393/1‑68 (MQ=255) cAATTGGCTCCAGCAGCTCAAACATCTCAATGTCAGTCTGATCCTGACGCGCATCAACGCGACCCGgc > 1:2369012/1‑68 (MQ=255) cAATTGGCTCCAGCAGCTCAAACATCTCAATGTCAGTCTGATCCTGACGCGCATCAACGCGACCCGgc > 1:1872412/1‑68 (MQ=255) cAATTGGCTCCAGCAGCTCAAACATCTCAATGTCAGTCTGATCCTGACGCGCATCAACGCGACCCGgc > 1:1822170/1‑68 (MQ=255) cAATTGGCTCCAGCAGCTCAAACATCTCAATGTCAGTCTGATCCTGACGCGCATCAACGCGACCCGgc > 1:686972/1‑68 (MQ=255) cAATTGGCTCCAGCAGCTCAAACATCTCAATGTCAGTCTGATCCTGACGCGCATCAACGCGACCCGgc > 1:137669/1‑68 (MQ=255) aTTGGCTCCAGCAGCTCAAACATCTCAATGTCAGTCTGATCCTGACGCGCATCAACGCGACCCGGCGCa < 1:273754/69‑1 (MQ=255) aTTGGCTCCAGCAGCTCAAACATCTCAATGTCAGTCTGATCCTGACGCGCATCAACGCGACCCGGCGCa < 1:762956/69‑1 (MQ=255) aTTGGCTCCAGCAGCTCAAACATCTCAATGTCAGTCTGATCCTGACGCGCATCAACGCGACCCGGCGCa < 1:803238/69‑1 (MQ=255) aTTGGCTCCAGCAGCTCAAACATCTCAATGTCAGCCTGATCCTGACGCGCATCAACGCGACCCGGCGCa < 1:2314606/69‑1 (MQ=255) ttGGCTCCAGCAGCTCAAACATCTCAATGTCAGTCTGATCCTGACGCGCATCAACGCGACCTGGCGCa < 1:441465/68‑1 (MQ=255) ttGGCTCCAGCAGCTCAAACATCTCAATGTCAGTCTGATCCTGACGCGCATCAACGCGACCCGGCGCa < 1:1193638/68‑1 (MQ=255) gcTCAAACATCTCAATGTCAGTCTGATCCTGACGCGCATCAACGCGACCCGGCGCAAACGGTACATGaa < 1:1489996/69‑1 (MQ=255) tcAATGTCTGTCTGATCCTGACGCGCATCAACGCGACCCGGCGCAAACGGTACATg < 1:680742/56‑1 (MQ=255) aaTGTCAGTCTGATCCTGACGCGCATCAACGCGACCCGGCGCa > 1:2830020/1‑43 (MQ=255) aaTGTCAGTCTGATCCTGACGCGCATCAACGCGACCCGGCGCa > 1:1074864/1‑43 (MQ=255) aaTGTCAGTCTGATCCTGACGCGCATCAACGCGACCCGGCGCa > 1:385370/1‑43 (MQ=255) aaTGTCAGTCTGATCCTGACGCGCATCAACGCGACCCGGCGCa > 1:1978040/1‑43 (MQ=255) aaTGTCAGTCTGATCCTGACGCGCATCAACGCGACCCGGCGCa > 1:2300201/1‑43 (MQ=255) tCAGTCTGATCCTGACGCGCATCAACGCGACCCGGCGCAAACGGTACATGAATGCTCAAACCTgcggcg > 1:894973/1‑69 (MQ=255) tCAGTCTGATCCTGACGCGCATCAACGCGACCCGGCGCAAACGGTACATGAATGCTCAAACCTgcggcg > 1:2287279/1‑69 (MQ=255) tcaTCCTGACGCGCATCAACGCGACCCGGCGCAAACGGTACATGAATGCTCAAACCTGCGGCGCTTGc > 1:1117798/3‑68 (MQ=255) tGATCCTGACGCGCATCAACGCGACCCGGCGCAAACGGTACATGAATGCTCAAACCTGCGGCGCTTGCg > 1:3033686/1‑69 (MQ=255) tGATCCTGACGCGCATCAACGCGACCCGGCGCAAACGGTACATGAATGCTCAAACCTGCGGCGCTTGCg > 1:1741586/1‑69 (MQ=255) tCCTGACGCGCATCAACGCGGCCCGGCGCAAACGGTACATGAATGc > 1:614155/1‑46 (MQ=255) tCCTGACGCGCATCAACGCGGCCCGGCGCAAACGGTACATGAATGc > 1:3134530/1‑46 (MQ=255) tCCTGACGCGCATCAACGCGGCCCGGCGCAAACGGTACATGAATGc > 1:3414982/1‑46 (MQ=255) tCCTGACGCGCATCAACGCGGCCCGGCGCAAACGGTACATGAATGc > 1:690814/1‑46 (MQ=255) tCCTGACGCGCATCAACGCGGCCCGGCGCAAACGGTACATGAATGc > 1:2613194/1‑46 (MQ=255) tCCTGACGCGCATCAACGCGGCCCGGCGCAAACGGTACATGAATGc > 1:310717/1‑46 (MQ=255) tCCTGACGCGCATCAACGCGGCCCGGCGCAAACGGTACATGAATGc > 1:870127/1‑46 (MQ=255) tCCTGACGCGCATCAACGCGGCCCGGCGCAAACGGTACATGAATGc > 1:293760/1‑46 (MQ=255) tCCTGACGCGCATCAACGCGGCCCGGCGCAAACGGTACATGAATGc > 1:906072/1‑46 (MQ=255) tCCTGACGCGCATCAACGCGGCCCGGCGCAAACGGTACATGAATGc > 1:1103380/1‑46 (MQ=255) cgtGACGCGCATCAACGCGGCCCGGCGCAAACGGTACATGAATGCTCAAACCTGCGGCGCTTGCGGCtt < 1:2985457/67‑1 (MQ=255) ccTGACGCGCATCAACGCGGCCCGGCGCAAACGGTACATGAATGCTCAAACCTGCGGCGCTTGCGGCtt < 1:2961765/69‑1 (MQ=255) ccTGACGCGCATCAACGCGGCCCGGCGCAAACGGTACATGAATGCTCAAACCTGCGGCGCTTGCGGCtt < 1:3342105/69‑1 (MQ=255) ccTGACGCGCATCAACGCGGCCCGGCGCAAACGGTACATGAATGCTCAAACCTGCGGCGCTTGCGGCtt < 1:3008953/69‑1 (MQ=255) ccTGACGCGCATCAACGCGGCCCGGCGCAAACGGTACATGAATGCTCAAACCTGCGGCGCTTGCGGCtt < 1:516740/69‑1 (MQ=255) ccTGACGCGCATCAACGCGGCCCGGCGCAAACGGTACATGAATGCTCAAACCTGCGGCGCTTGCGGCtt < 1:285820/69‑1 (MQ=255) ccTGACGCGCATCAACGCGGCCCGGCGCAAACGGTACATGAATGCTCAAACCTGCGGCGCTTGCGGCtt < 1:1020425/69‑1 (MQ=255) ccTGACGCGCATCAACGCGGCCCGGCGCAAACGGTACATGAATGCTCAAACCTGCGGCGCTTGCGGCtt < 1:2400823/69‑1 (MQ=255) ccTGACGCGCATCAACGCGGCCCGGCGCAAACGGTACATGAATGCTCAAACCTGCGGCGCTTGCGGCtt < 1:224266/69‑1 (MQ=255) ccTGACGCGCATCAACGCGGCCCGGCGCAAACGGTACATGAATGCTCAAACCTGCGGCGCTTGCGGCtt < 1:2100166/69‑1 (MQ=255) ccTGACGCGCATCAACGCGGCCCGGCGCAAACGGTACATGAATGCTCAAACCTGCGGCGCTTGCGGCtt < 1:2045484/69‑1 (MQ=255) ccTGACGCGCATCAACGCGGCCCGGCGCAAACGGTACATGAATGCTCAAACCTGCGGCGCTTGCGGCtt < 1:1927734/69‑1 (MQ=255) ccTGACGCGCATCAACGCGGCCCGGCGCAAACGGTACATGAATGCTCAAACCTGCGGCGCTTGCGGCtt < 1:829789/69‑1 (MQ=255) ccTGACGCGCATCAACGCGACCCGGCGCAAACGGTACATGAATGCTCAAACCTGCGGCGCTTGCGGCt < 1:2808613/68‑1 (MQ=255) ccTGACGCGCATCAACGCGACCCGGCGCAAACGGTACATGAATGCTCAAACCTGCGGCGCTTGCGGCt < 1:994929/68‑1 (MQ=255) ccTGACGCGCATCAACGCGACCCGGCGCAAACGGTACATGAATGCTCAAACCTGCGGCGCTTGCGGCt < 1:1647578/68‑1 (MQ=255) ccTGACGCGCATCAACGCGACCCGGCGCAAACGGTACATGAATGCTCAAACCTGCGGCGCTTGCGGCt < 1:1696277/68‑1 (MQ=255) cTGACGCGCATCAACGCGGCCCGGCGCAAACGGTACATGAATGCTCAAACCTGCGGCGCTTGCGGCtt < 1:579049/68‑1 (MQ=255) cTGACGCGCATCAACGCGACCCGGCGCAAACGGTACATGAATGCTCAAACCTGCGGCGCTTGCGGCt < 1:3241502/67‑1 (MQ=255) cTGACGCGCATCAACGCGACCCGGCGCAAACGGTACATGAATGCTCAAACCTGCGGCGCTTGCGGCt < 1:187665/67‑1 (MQ=255) gcgcATCAACGCGACCCGGCGCAAACGGTACATGAATGCTCAAACCTGCGGCGCTTGCGGCTTtctc > 1:495223/1‑67 (MQ=255) gcgcATCAACGCGACCCGGCGCAAACGGTACATGAATGCTCAAACCTGCGGCGCTTGCGGCTTTCTCaa > 1:3013705/1‑69 (MQ=255) gcgcATCAACGCGACCCGGCGCAAACGGTACATGAATGCTCAAACCTGCGGCGCTTGCGGCTTTCTCaa > 1:665671/1‑69 (MQ=255) gcgcATCAACGCGACCCGGCGCAAACGGTACATGAATGCTCAAACCTGCGGCGCTTGCGGCTTTCTCaa > 1:2837961/1‑69 (MQ=255) gcgcATCAACGCGACCCGGCGCAAACGGTACATGAATGCTCAAACCTGCGGCGCTTGCGGCTTTCTCaa < 1:3022405/69‑1 (MQ=255) gcgcATCAACGCGACCCGGCGCAAACGGTACATGAATGCTCAAACCTGCGGCGCTTGCGGCTTTCTCaa > 1:2961763/1‑69 (MQ=255) aaCGCGACCCGGCGCAAACGGTACATGAATGCTCAAACCTGCGGCGCTTGCGGCttt > 1:760459/1‑57 (MQ=255) aaCGCGACCCGGCGCAAACGGTACATGAATGCTCAAACCTGCGGCGCTTGCGGCTTtctc < 1:754371/60‑1 (MQ=255) aaCGCGACCCGGCGCAAACGGTACATGAATGCTCAAACCTGCGGCGCTTGCGGCTTtctc < 1:1073523/60‑1 (MQ=255) aaCGCGACCCGGCGCAAACGGTACATGAATGCTCAAACCTGCGGCGCTTGCGGCTTtctc < 1:1220481/60‑1 (MQ=255) aaCGCGACCCGGCGCAAACGGTACATGAATGCTCAAACCTGCGGCGCTTGCGGCTTtctc < 1:1359476/60‑1 (MQ=255) aaCGCGACCCGGCGCAAACGGTACATGAATGCTCAAACCTGCGGCGCTTGCGGCTTtctc < 1:2613097/60‑1 (MQ=255) aaCGCGACCCGGCGCAAACGGTACATGAATGCTCAAACCTGCGGCGCTTGCGGCTTtctc < 1:1664401/60‑1 (MQ=255) aaCGCGACCCGGCGCAAACGGTACATGAATGCTCAAACCTGCGGCGCTTGCGGCTTtctc < 1:2937849/60‑1 (MQ=255) aaCGCGACCCGGCGCAAACGGTACATGAATGCTCAAACCTGCGGCGCTTGCGGCTTtctc < 1:2145877/60‑1 (MQ=255) aaCGCGACCCGGCGCAAACGGTACATGAATGCTCAAACCTGCGGCGCTTGCGGCTTTCTCAACaccaac > 1:2111436/1‑69 (MQ=255) aaCGCGACCCGGCGCAAACGGTACATGAATGCTCAAACCTGCGGCGCTTGCGGCTTTCTCAACaccaac > 1:1237167/1‑69 (MQ=255) cgACCCGGCGCAAACGGTACATGAATGCTCAAACCTGCGGCGCTTGCGGCTTTCTCAACACCAACcaca < 1:3278934/69‑1 (MQ=255) cgACCCGGCGCAAACGGTACATGAATGCTCAAACCTGCGGCGCTTGCGGCTTTCTCAACACCAACcaca < 1:1137828/69‑1 (MQ=255) cgACCCGGCGCAAACGGTACATGAATGCTCAAACCTGCGGCGCTTGCGGCTTTCTCAACACCAACcaca < 1:112991/69‑1 (MQ=255) cgACCCGGCGCAAACGGTACATGAATGCTCAAACCTGCGGCGCTTGCGGCTTTCTCAACACCAACcaca < 1:2830964/69‑1 (MQ=255) | CCGTCAGCAATTGGCTCCAGCAGCTCAAACATCTCAATGTCAGTCTGATCCTGACGCGCATCAACGCGACCCGGCGCAAACGGTACATGAATGCTCAAACCTGCGGCGCTTGCGGCTTTCTCAACACCAACCACA > minE/2296338‑2296472 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |