Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,575,885 A→G 43.8% V520V (GTA→GTG glyS → glycine tRNA synthetase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,575,8850AG43.8% 28.9 / 102.4 127V520V (GTA→GTGglySglycine tRNA synthetase, beta subunit
Reads supporting (aligned to +/- strand):  ref base A (19/52);  new base G (36/19);  total (55/72)
Fisher's exact test for biased strand distribution p-value = 2.46e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

Only 100 of 128 total aligned reads displayed.

CTTGATCTGCAAACGCTGACCGAAGAAGCGGTGCGTCTGTATGGCGATAAGCTGACTAATGCCAACGTAGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCAGGACGAAGGTTATACC  >  minE/2575817‑2575951
                                                                    |                                                                  
cTTGATCTGCAAACGCTGACCGAAGAAGCGGTGCGTCTGTATGGCGATAAGCTGACTAATGCCAACGTa                                                                    >  1:603254/1‑69 (MQ=255)
     tCTGCAAACGCTGACCGAAGAAGCGGTGCGTCTGTATGGCGATAAGCTGACTAATGCCAACGTAGTtg                                                                <  1:538395/68‑1 (MQ=255)
     tCTGCAAACGCTGACCGAAGAAGCGGTGCGTCTGTATGGCGATAAGCTGACTAATGCCAACGTAGTtg                                                                <  1:3392923/68‑1 (MQ=255)
     tCTGCAAACGCTGACCGAAGAAGCGGTGCGTCTGTATGGCGATAAGCTGACTAATGCCAACGTAGTtg                                                                <  1:3192420/68‑1 (MQ=255)
     tCTGCAAACGCTGACCGAAGAAGCGGTGCGTCTGTATGGCGATAAGCTGACTAATGCCAACGTAGTtg                                                                <  1:204046/68‑1 (MQ=255)
     tCTGCAAACGCTGACCGAAGAAGCGGTGCGTCTGTATGGCGATAAGCTGACTAATGCCAACGTAGTtg                                                                <  1:3031469/68‑1 (MQ=255)
     tCTGCAAACGCTGACCGAAGAAGCGGTGCGTCTGTATGGCGATAAGCTGACTAATGCCAACGTAGTtg                                                                <  1:1582012/68‑1 (MQ=255)
         cAAACGCTGACCGAAGAAGCGGTGCGTCTGTATGGCGATAAGCTGACTAATGCCAACGTAGTTgatgat                                                           >  1:522237/1‑69 (MQ=255)
         cAAACGCTGACCGAAGAAGCGGTGCGTCTGTATGGCGATAAGCTGACTAATGCCAACGTAGTTgatgat                                                           >  1:2162804/1‑69 (MQ=255)
         cAAACGCTGACCGAAGAAGCGGTGCGTCTGTATGGCGATAAGCTGACTAATGCCAACGTAGTTgatgat                                                           >  1:2284143/1‑69 (MQ=255)
                tGACCGAAGAAGCAGTGCGTCTGTATGGCGATAAGCTGACTAACGCCAACGTGGTTGATGATGTTATc                                                     >  1:2803865/1‑68 (MQ=255)
                tGACCGAAGAAGCAGTGCGTCTGTATGGCGATAAGCTGACTAACGCCAACGTGGTTGATGATGTTATc                                                     >  1:1852396/1‑68 (MQ=255)
                tGACCGAAGAAGCAGTGCGTCTGTATGGCGATAAGCTGACTAACGCCAACGTGGTTGATGATGTTATc                                                     >  1:1953432/1‑68 (MQ=255)
                tGACCGAAGAAGCAGTGCGTCTGTATGGCGATAAGCTGACTAACGCCAACGTGGTTGATGATGTTATc                                                     >  1:1966772/1‑68 (MQ=255)
                tGACCGAAGAAGCAGTGCGTCTGTATGGCGATAAGCTGACTAACGCCAACGTGGTTGATGATGTTATc                                                     >  1:2331240/1‑68 (MQ=255)
                tGACCGAAGAAGCAGTGCGTCTGTATGGCGATAAGCTGACTAACGCCAACGTGGTTGATGATGTTATc                                                     >  1:2555740/1‑68 (MQ=255)
                tGACCGAAGAAGCAGTGCGTCTGTATGGCGATAAGCTGACTAACGCCAACGTGGTTGATGATGTTATc                                                     >  1:2705100/1‑68 (MQ=255)
                tGACCGAAGAAGCAGTGCGTCTGTATGGCGATAAGCTGACTAACGCCAACGTGGTTGATGATGTTATc                                                     >  1:1091088/1‑68 (MQ=255)
                tGACCGAAGAAGCAGTGCGTCTGTATGGCGATAAGCTGACTAACGCCAACGTGGTTGATGATGTTATc                                                     >  1:3062503/1‑68 (MQ=255)
                tGACCGAAGAAGCAGTGCGTCTGTATGGCGATAAGCTGACTAACGCCAACGTGGTTGATGATGTTATc                                                     >  1:675554/1‑68 (MQ=255)
                tGACCGAAGAAGCAGTGCGTCTGTATGGCGATAAGCTGACTAACGCCAACGTGGTTGATGATGTTATc                                                     >  1:947011/1‑68 (MQ=255)
                tGACCGAAGAAGCAGTGCGTCTGTATGGCGATAAGCTGACTAACGCCAACGTGGTTGATGATGTTATc                                                     >  1:97867/1‑68 (MQ=255)
                   ccGAAGAAGCGGTGCGTCTGTATGGCGATAAGCTGACTAATGCCAACGTAGTtg                                                                <  1:1238307/54‑1 (MQ=255)
                                     tGTATGGCGATAAGCTGACTAATGCCAACGTAGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTcc                               >  1:1628033/1‑69 (MQ=255)
                                           gCGATAAGCTGACTAATGCCAACGTAGTTGATGATGTTATCGACTTTATGCTCGGTCGCt                                  >  1:2349632/1‑60 (MQ=255)
                                           gCGATAAGCTGACTAATGCCAACGTAGTTGATGATGTTATCGACTTTATGCTCGGTCGCt                                  >  1:306422/1‑60 (MQ=255)
                                           gCGATAAGCTGACTAATGCCAACGTAGTTGATGATGTTATCGACTTTATGCTCGGTCGCt                                  >  1:887786/1‑60 (MQ=255)
                                           gCGATAAGCTGACTAATGCCAACGTAGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCt                         >  1:1783331/1‑69 (MQ=255)
                                                   cTGACTAACGCCAACGTGGTTGATGATGTTATCGACTTTATTCTCGGTCGCTTCCGCGCCTGGTATCa                  <  1:2567687/68‑1 (MQ=255)
                                                   cTGACTAACGCCAACGTGGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATct                  <  1:1743845/68‑2 (MQ=255)
                                                   cTGACTAACGCCAACGTGGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCa                  <  1:1492207/68‑1 (MQ=255)
                                                   cTGACTAACGCCAACGTGGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCa                  <  1:2617712/68‑1 (MQ=255)
                                                   cTGACTAACGCCAACGTGGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCa                  <  1:1967913/68‑1 (MQ=255)
                                                   cTGACTAACGCCAACGTGGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCa                  <  1:944810/68‑1 (MQ=255)
                                                   cTGACTAACGCCAACGTGGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCa                  <  1:1696816/68‑1 (MQ=255)
                                                   cTGACTAACGCCAACGTGGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCa                  <  1:3024256/68‑1 (MQ=255)
                                                   cTGACTAACGCCAACGTGGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCa                  <  1:1304114/68‑1 (MQ=255)
                                                   cTGACTAACGCCAACGTGGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCa                  <  1:1373532/68‑1 (MQ=255)
                                                   cTGACTAACGCCAACGTGGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCa                  <  1:2198278/68‑1 (MQ=255)
                                                   cTGACTAACGCCAACGTGGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCa                  <  1:1834727/68‑1 (MQ=255)
                                                    tGACTAACGCCTACGTGGTTGATGATGTTATCGACTTTATGCTCGGTAGCTTCCGCGCCTGGTATCa                  <  1:3147531/67‑1 (MQ=255)
                                                    tGACTAACGCCAACGTGGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCa                  <  1:2339062/67‑1 (MQ=255)
                                                    tGACTAACGCCAACGTGGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCa                  <  1:366804/67‑1 (MQ=255)
                                                         aacgCCAACGTGGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCAGGACGaa           >  1:2775248/4‑69 (MQ=255)
                                                         aacgCCAACGTGGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCAGGACGaa           >  1:2770706/4‑69 (MQ=255)
                                                         aacgCCAACGTGGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCAGGACGaa           >  1:1274057/4‑69 (MQ=255)
                                                         aacgCCAACGTGGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCAGGACGaa           >  1:2623854/4‑69 (MQ=255)
                                                         aacgCCAACGTGGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCAGGACGaa           >  1:307633/4‑69 (MQ=255)
                                                         aacgCCAACGTGGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCAGGACGaa           >  1:3245841/4‑69 (MQ=255)
                                                         aacgCCAACGTGGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCAGGACGaa           >  1:1150618/4‑69 (MQ=255)
                                                         aacgCCAACGTGGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCAGGACGaa           >  1:252361/4‑69 (MQ=255)
                                                         aacgCCAACGTGGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCAGGACGaa           >  1:2615701/4‑69 (MQ=255)
                                                         aacgCCAACGTGGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCAGGACGaa           >  1:1106274/4‑69 (MQ=255)
                                                         aacgCCAACGTGGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCAGGACGaa           >  1:50883/4‑69 (MQ=255)
                                                         aacgCCAACGTGGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCAGGACGaa           >  1:2187884/4‑69 (MQ=255)
                                                         aacgCCAACGTGGTTGATGATGATATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCAg                 >  1:2967025/4‑63 (MQ=255)
                                                            gCCAACGTAGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCAGGACGAAGGt        <  1:651976/69‑1 (MQ=255)
                                                            gCCAACGTAGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCAGGACGAAGGt        <  1:626554/69‑1 (MQ=255)
                                                            gCCAACGTAGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCAGGACGAAGGt        <  1:803623/69‑1 (MQ=255)
                                                            gCCAACGTAGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCAGGACGAAGGt        <  1:603955/69‑1 (MQ=255)
                                                            gCCAACGTAGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCAGGACGAAGGt        <  1:1185174/69‑1 (MQ=255)
                                                            gCCAACGTAGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCAGGACGAAGGt        <  1:789581/69‑1 (MQ=255)
                                                            gCCAACGTAGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCAGGACGAAGGt        <  1:3388790/69‑1 (MQ=255)
                                                            gCCAACGTAGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCAGGACGAAGGt        <  1:582194/69‑1 (MQ=255)
                                                            gCCAACGTAGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCAGGACGAAGGt        <  1:355645/69‑1 (MQ=255)
                                                            gCCAACGTAGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCAGGACGAAGGt        <  1:47945/69‑1 (MQ=255)
                                                            gCCAACGTAGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCAGGACGAAGGt        <  1:413059/69‑1 (MQ=255)
                                                            gCCAACGTAGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCAGGACGAAGGt        <  1:775526/69‑1 (MQ=255)
                                                            gCCAACGTAGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCAGGACGAAGGt        <  1:313865/69‑1 (MQ=255)
                                                            gCCAACGTAGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCAGGACGAAGGt        <  1:3090200/69‑1 (MQ=255)
                                                            gCCAACGTAGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCAGGACGAAGGt        <  1:1766031/69‑1 (MQ=255)
                                                            gCCAACGTAGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCAGGACGAAGGt        <  1:858087/69‑1 (MQ=255)
                                                            gCCAACGTAGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCAGGACGAAGGt        <  1:2840900/69‑1 (MQ=255)
                                                            gCCAACGTAGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCAGGACGAAGGt        <  1:1368866/69‑1 (MQ=255)
                                                            gCCAACGTAGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCAGGACGAAGGt        <  1:2593033/69‑1 (MQ=255)
                                                            gCCAACGTAGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCAGGACGAAGGt        <  1:2492678/69‑1 (MQ=255)
                                                            gCCAACGTAGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCAGGACGAAGGt        <  1:2467510/69‑1 (MQ=255)
                                                            gCCAACGTAGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCAGGACGAAGGt        <  1:3145334/69‑1 (MQ=255)
                                                            gCCAACGTAGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCAGGACGAAGGt        <  1:1027047/69‑1 (MQ=255)
                                                            gCCAACGTAGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCAGGACGAAGGt        <  1:1661576/69‑1 (MQ=255)
                                                            gCCAACGTAGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCAGGACGAAGGt        <  1:171748/69‑1 (MQ=255)
                                                            gCCAACGTAGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCAGGACGAAGGt        <  1:2019436/69‑1 (MQ=255)
                                                            gCCAACGTAGTTGATGATGTTATCCACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCAGGACGAAGGt        <  1:2089741/69‑1 (MQ=255)
                                                             ccAACGTAGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCAGGACGAAGGt        <  1:2416949/68‑1 (MQ=255)
                                                             acAACGTAGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCAGGACGAAGGt        >  1:1705949/2‑68 (MQ=255)
                                                               aaCGTAGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCAGGACGAAGGTtat     <  1:717683/69‑1 (MQ=255)
                                                               aaCGTAGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCAGGACGAAGGTtat     <  1:8769/69‑1 (MQ=255)
                                                               aaCGTAGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCAGGACGAAGGTtat     <  1:1304808/69‑1 (MQ=255)
                                                               aaCGTAGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCAGGACGAAGGTtat     <  1:1231038/69‑1 (MQ=255)
                                                               aaCGTAGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCAGGACGAAGGTtat     <  1:402800/69‑1 (MQ=255)
                                                               aaCGTAGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCAGGACGAAGGTtat     <  1:1308664/69‑1 (MQ=255)
                                                                aCGTAGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCAGGACGAAGGTtat     <  1:3285477/68‑1 (MQ=255)
                                                                  gTAGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCAGGACGAAgg         >  1:329841/1‑62 (MQ=255)
                                                                  gTAGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCAGGACGAAGGTTATAcc  >  1:632521/1‑69 (MQ=255)
                                                                  gTAGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCAGGACGAAGGTTATAcc  <  1:870490/69‑1 (MQ=255)
                                                                  gTAGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCAGGACGAAGGTTATAcc  >  1:154809/1‑69 (MQ=255)
                                                                  gTAGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCAGGACGAAGGTTATAcc  <  1:1975007/69‑1 (MQ=255)
                                                                  gTAGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCAGGACGAAGGTTATAcc  <  1:1858207/69‑1 (MQ=255)
                                                                  gTAGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCAGGACGAAGGTTATAcc  <  1:1849903/69‑1 (MQ=255)
                                                                  gTAGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCAGGACGAAGGTTATAcc  >  1:1777936/1‑69 (MQ=255)
                                                                    |                                                                  
CTTGATCTGCAAACGCTGACCGAAGAAGCGGTGCGTCTGTATGGCGATAAGCTGACTAATGCCAACGTAGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCGCCTGGTATCAGGACGAAGGTTATACC  >  minE/2575817‑2575951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: